DOT Drug Testing - Center Hill, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 36 testing centers in the Center Hill area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

36 DOT Drug-Testing Centers in Center Hill

Minutes Away, Test Today

117 W Belt Ave Ste A

7.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
117 W Belt Ave Ste A
Bushnell, FL 33513

1389 Se Us Hwy 301

9.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1389 Se Us Hwy 301
Sumterville, FL 33585

101 S 11th St Ste 2,

12.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
101 S 11th St Ste 2,
Leesburg, FL 34748

601 E Dixie Ave Ste 804

13.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
601 E Dixie Ave Ste 804
Leesburg, FL 34748

801 E Dixie Ave Suite 105a,

13.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
801 E Dixie Ave Suite 105a,
Leesburg, FL 34748

210 S Lake St Ste 4

13.41 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
210 S Lake St Ste 4
Leesburg, FL 34748

2500 Citrus Blvd

14.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2500 Citrus Blvd
Leesburg, FL 34748

34498 Cortez Blvd

14.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
34498 Cortez Blvd
Ridge Manor, FL 33523

1501 N Us Highway 441

15.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1501 N Us Highway 441
The Villages, FL 32159

245 Citrus Tower Blvd, Suite 203

16.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
245 Citrus Tower Blvd, Suite 203
Clermont, FL 34711

31075 Cortez Blvd

16.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
31075 Cortez Blvd
Brooksville, FL 34602

910 Old Camp Rd Bldg 110 Ste 112

17.72 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
910 Old Camp Rd Bldg 110 Ste 112
The Villages, FL 32162

1099 Citrus Tower Blvd

17.72 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1099 Citrus Tower Blvd
Clermont, FL 34711

1920 Don Wickham Dr Ste 205

17.87 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1920 Don Wickham Dr Ste 205
Clermont, FL 34711

11962 County Road 101 Ste 402 The Villa

18.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11962 County Road 101 Ste 402 The Villa
Lady Lake, FL 32162

8600a County Road 466. Ste A

18.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8600a County Road 466. Ste A
The Villages, FL 32162

3120 Waterman Way

18.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3120 Waterman Way
Tavares, FL 32778

2105 Hartwood Marsh Road, Suite 6a

19.71 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-2:00 PM • Sat: 6:30 AM-11:30 AM✓ Walk-in with Electronic Donor Pass
2105 Hartwood Marsh Road, Suite 6a
Clermont, FL 34711

805 E County Rd 466

20.38 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
805 E County Rd 466
Lady Lake, FL 32159

1300 E Burleigh Blvd

20.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1300 E Burleigh Blvd
Tavares, FL 32778

15701 State Road 50 Ste 101

21.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
15701 State Road 50 Ste 101
Clermont, FL 34711

628 Cagan View Rd Ste 3 Ste 3

21.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
628 Cagan View Rd Ste 3 Ste 3
Clermont, FL 34714

17820 Se 109 Avenue, Suite 106a

21.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
17820 Se 109 Avenue, Suite 106a
Summerfield, FL 34491

2130 Prevatt St, Suite B

22.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2130 Prevatt St, Suite B
Eustis, FL 32726

13933 17th St Ste 101

23.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
13933 17th St Ste 101
Dade City, FL 33525

210 S. Apopka Ave,

24.02 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-2:00 PM • Sat: 6:00 AM-10:00 AM✓ Walk-in with Electronic Donor Pass
210 S. Apopka Ave,
Inverness, FL 34452

312 S. Line Ave., Ste B

24.36 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
312 S. Line Ave., Ste B
Inverness, FL 34453

20205 Cortez Blvd

25.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
20205 Cortez Blvd
Brooksville, FL 34601

3005 Daniels Rd

25.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3005 Daniels Rd
Winter Garden, FL 34787

31450 Church St

28.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
31450 Church St
Sorrento, FL 32776

17240 Cortez Blvd

28.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
17240 Cortez Blvd
Brooksville, FL 34601

2700 Old Winter Garden Rd

28.79 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2700 Old Winter Garden Rd
Ocoee, FL 34761

1151 Blackwood Ave Ste 160

28.84 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1151 Blackwood Ave Ste 160
Ocoee, FL 34761

10131 W Colonial Dr, Bldg. C, Ste 8

29.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10131 W Colonial Dr, Bldg. C, Ste 8
Ocoee, FL 34761

10762 Se Us Highway 441

29.13 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10762 Se Us Highway 441
Belleview, FL 34420

37802 Medical Arts Ct

29.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
37802 Medical Arts Ct
Zephyrhills, FL 33541

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 36 DOT drug testing locations to serve you in and around Center Hill, FL.

In and around Center Hill, FL, our accredited facilities offer comprehensive DOT drug and alcohol testing services, including physicals and random testing program enrollment. We support employers, solo operators, and all DOT modes under the 49 CFR Part 40 requirements, which includes FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Our testing centers are conveniently located nearby with available same-day service.

Whether for personal, employee, or another individual’s testing, our process is straightforward. Select the required DOT service and a convenient location. Scheduling a DOT drug and alcohol test near Center Hill is intuitive and hassle-free.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Center Hill, Florida DOT Services

DOT Drug Testing & Services Available in Center Hill, FL:

Our DOT services in Center Hill, FL, are comprehensive, covering drug and alcohol testing requirements for all regulated employees. Accredited Drug Testing's mission in Center Hill, Florida, is to ensure a safer, compliant work environment for all transportation industry stakeholders.

DOT Services in Center Hill, FL

DOT regulations play a crucial role in promoting safety for employees in Center Hill, FL, through stringent drug and alcohol screening protocols. These regulations are designed to maintain a drug-free workplace, ensuring both individual and public safety.

Employers in Center Hill, Florida, are required to implement a comprehensive testing program that adheres to DOT standards, aimed at identifying substance abuse issues amongst employees, thereby mitigating risks in the workplace.

Regular screenings mandated by DOT rules help deter substance use, providing employees with a safer, more reliable work environment in Center Hill, FL.

Training and education are also part of DOT's approach, equipping employers and employees in Center Hill, Florida, with knowledge to identify and respond to drug and alcohol-related issues effectively.

FMCSA Information

In Center Hill, FL, FMCSA rules mandate regular drug tests for CDL holders, maintaining road safety.

FMCSA guidelines require random screenings, reinforcing safety standards in Center Hill, Florida.

USCG Information

In Center Hill, FL, USCG drug tests are crucial for marine safety for crew members.

USCG alcohol regulations in Center Hill, Florida, help uphold maritime safety standards.

FAA Information

FAA mandates in Center Hill, FL, require drug tests for aviation employees, enhancing flight safety.

Compliance with FAA alcohol testing rules is crucial for aviation safety in Center Hill, Florida.

FRA Information

In Center Hill, FL, FRA regulations enforce drug screening for rail workers for safe rail operations.

FRA alcohol testing standards in Center Hill, Florida, help maintain railway safety and compliance.

FTA Information

FTA rules in Center Hill, FL, ensure transit employee safety through required drug screenings.

Alcohol testing under FTA enhances transit system safety and reliability in Center Hill, Florida.

PHMSA Information

PHMSA regulations in Center Hill, FL, ensure safety via mandatory drug tests for pipeline workers.

Random alcohol tests are vital under PHMSA rules in Center Hill, Florida, for public safety assurance.

Scheduling in Center Hill, FL

To arrange a DOT drug or alcohol test in Center Hill, call (800) 221-4291, or use our online scheduling system. Make sure to complete the registration form before your visit.

Enter your zip code, and we'll locate the nearest Center Hill testing center for your chosen test. You'll receive a donor pass with vital instructions by email. Bring this form or display it on your smartphone at the center. Usually, no appointments are needed, but ensure you complete the donor details and payment during registration.

Our labs have SAMHSA certification, and licensed physicians, serving as Medical Review Officers, verify all results.

We offer DOT drug and alcohol testing in Center Hill and nearby areas, ensuring accessible service no matter where you are.

Whether traveling or at home, Accredited Drug Testing provides nationwide scheduling, anytime and anywhere!

Florida Dept. of Transportation Updates

DUI Statistics in Center Hill, FL

Center Hill, situated in Sumter County, Florida, is part of a state that grapples with significant DUI-related challenges. Florida, abbreviated as FL, reports thousands of DUI arrests annually, with a notable portion occurring in smaller communities like those found in Sumter County. Law enforcement in FL, including in Center Hill, focuses heavily on DUI prevention to reduce accidents and fatalities. DUI checkpoints and awareness campaigns are common methods used to combat this issue. While detailed statistics specific to Center Hill are less frequently published, the broader trends in Sumter County align with the state's efforts to mitigate the impact of DUI incidents.

Drug-Involved Accidents in Sumter County, FL

In Sumter County, where Center Hill is located, drug-involved accidents are a significant concern. Florida (FL) has been active in tracking and addressing these incidents, which often include substances beyond alcohol. The Florida Department of Transportation (FDOT) collaborates with local agencies to implement strategies aimed at reducing drug-related traffic incidents. These initiatives are vital in communities like Center Hill, FL, where the impact of such accidents can be profoundly felt. FL state programs focus on education and prevention, aiming to make roadways safer and to decrease the number of crashes linked to drug impairment.

Marijuana-Related Accidents in Center Hill, FL

With the evolving legal landscape surrounding marijuana in Florida (FL), marijuana-related traffic incidents have gained heightened attention. Center Hill, part of Sumter County, FL, is not exempt from these challenges. As FL legalized medical marijuana, there has been concern about its effect on driving safety. The Department of Transportation in Florida is analyzing accident data to understand the correlation between marijuana use and road safety. Law enforcement in places like Center Hill, FL, is trained to recognize impairment signs, and public safety campaigns are launched to educate drivers on the risks of driving under the influence of marijuana.

Center Hill Drug Testing

Quickly find trusted local drug testing centers in Center Hill, FL — fast, convenient, and reliable every time!

Center Hill DOT Drug Testing

Quickly find a local DOT drug testing center in Center Hill, FL — fast, reliable, convenient nationwide service!

Center Hill DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Center Hill, FL.

Center Hill Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Center Hill, FL.v

Center Hill Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Center Hill, FL locations—results you can trust, every time!

Center Hill Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Center Hill, FL.

Center Hill Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Center Hill, FL.

Center Hill 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Center Hill, FL employers and individuals nationwide.

Center Hill 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Center Hill, FL workplace and personal testing needs.

Center Hill On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Center Hill, FL workplace compliant.

Center Hill DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Center Hill, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291