DOT Drug Testing - Highland, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 39 testing centers in the Highland area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

39 DOT Drug-Testing Centers in Highland

Minutes Away, Test Today

345 W Madison St

12.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
345 W Madison St
Starke, FL 32091

12 W Macclenny Ave

12.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12 W Macclenny Ave
Macclenny, FL 32063

159 N 3rd St

12.76 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
159 N 3rd St
Macclenny, FL 32063

1708 Blanding Blvd

12.99 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
1708 Blanding Blvd
Middleburg, FL 32068

1437 S 6th St

13.39 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-6:00 PM✓ Walk-in with Electronic Donor Pass
1437 S 6th St
Macclenny, FL 32063

1550 S Water St

13.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1550 S Water St
Starke, FL 32091

2140 Kingsley Ave

18.48 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
2140 Kingsley Ave
Orange Park, FL 32073

2020 Kingsley Ave Ste A

18.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2020 Kingsley Ave Ste A
Orange Park, FL 32073

1895 Kingsley Ave Ste 803

18.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1895 Kingsley Ave Ste 803
Orange Park, FL 32073

1563 Kingsley Ave Ste 102

19.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1563 Kingsley Ave Ste 102
Orange Park, FL 32073

1465 Kingsley Ave Ste 1401,

19.45 mi
🕑 Testing Hours: Mon–Fri: 12:00 PM-2:00 PM✓ Walk-in with Electronic Donor Pass
1465 Kingsley Ave Ste 1401,
Orange Park, FL 32073

Onsite Only

20.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Onsite Only
Fleming Island, FL 32003

7963 Normandy Blvd

20.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7963 Normandy Blvd
Jacksonville, FL 32221

1680 Eagle Harbor Parkway, Suite B

20.39 mi
🕑 Testing Hours: Mon–Fri: 12:00 PM-2:00 PM • Sat: 8:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
1680 Eagle Harbor Parkway, Suite B
Orange Park, FL 32003

1584 Normandy Village Parkway Ste 32

20.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1584 Normandy Village Parkway Ste 32
Jacksonville, FL 32221

6671 Hyde Grove Ave

21.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6671 Hyde Grove Ave
Jacksonville, FL 32210

850 E Main St Attn Lab

21.54 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
850 E Main St Attn Lab
Lake Butler, FL 32054

5964 Normandy Blvd

22.22 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
5964 Normandy Blvd
Jacksonville, FL 32205

3185 Highway 17

22.47 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3185 Highway 17
Green Cove Springs, FL 32043

4616 San Juan Ave

22.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4616 San Juan Ave
Jacksonville, FL 32210

1912 Hamilton St Ste 103

22.65 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1912 Hamilton St Ste 103
Jacksonville, FL 32210

1610 Blanding Blvd

22.77 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1610 Blanding Blvd
Jacksonville, FL 32210

5501 Roosevelt Blvd

22.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5501 Roosevelt Blvd
Jacksonville, FL 32244

7033 Commonwealth Ave Ste 8

22.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7033 Commonwealth Ave Ste 8
Jacksonville, FL 32220

705 Ferris Street

23.31 mi
🕑 Testing Hours: M-Th 8:00 AM-5:00 PM • F 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
705 Ferris Street
Green Cove Springs, FL 32043

128 Nw 137th Dr

23.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
128 Nw 137th Dr
Newberry, FL 32669

837 Edgewood Ave N

24.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
837 Edgewood Ave N
Jacksonville, FL 32254

1718 Edgewood Ave N

24.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1718 Edgewood Ave N
Jacksonville, FL 32254

12303 San Jose Blvd

24.81 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
12303 San Jose Blvd
Jacksonville, FL 32223

2063 Oak St

25.72 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-4:00 PM • Sat: 10:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
2063 Oak St
Jacksonville, FL 32204

4498 Hendricks Ave

26.13 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
4498 Hendricks Ave
Jacksonville, FL 32207

9826 San Jose Blvd

26.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9826 San Jose Blvd
Jacksonville, FL 32257

9857 Old Saint Augustine Rd Ste 4

26.45 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9857 Old Saint Augustine Rd Ste 4
Jacksonville, FL 32257

2545 Riverside Ave Ste 3

26.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2545 Riverside Ave Ste 3
Jacksonville, FL 32205

231 Airline Way

26.61 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
231 Airline Way
Jacksonville, FL 32217

1325 San Marco Blvd Ste 301

26.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1325 San Marco Blvd Ste 301
Jacksonville, FL 32207

4221 Baymeadows Rd Ste 6

27.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4221 Baymeadows Rd Ste 6
Jacksonville, FL 32217

300 W Adams St Ste 240

27.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
300 W Adams St Ste 240
Jacksonville, FL 32202

47 W Adams St

27.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
47 W Adams St
Jacksonville, FL 32202

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 39 DOT drug testing locations to serve you in and around Highland, FL.

Our Highland, FL-based DOT drug testing facilities cater to the needs of employers, single operators, and all DOT modes under 49 CFR Part 40 including FMCSA, FTA, FAA, USCG, FRA, and PHMSA. We offer services such as DOT Drug and Alcohol Testing, DOT Physicals, Random pool enrollment, MIS and MVR reports, and driver qualifications. Conveniently located minutes from home or office, most services are available same day.

Choose the necessary DOT service or drug/alcohol testing by selecting a nearby location for you, your employee, or another. Our process is designed for simplicity, enabling seamless scheduling of DOT drug tests in Highland.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Highland, Florida DOT Services

DOT Drug Testing & Services Available in Highland, FL:

In Highland, FL, our DOT services encompass a wide range of essential testing—including drug and alcohol checks—ensuring compliance and enhancing safety for both employees and the community.

DOT Services in Highland, FL

DOT regulations mandate stringent drug and alcohol screening for safety. Compliance ensures safer operations in Highland, FL. Testing helps identify issues affecting workplace safety standards and employee efficiency.

Highland, Florida employers must adhere to DOT standards for employee screenings. Regular testing mitigates accidents and enhances public safety. DOT policies enforce a zero-tolerance towards drug/alcohol use.

Diligent testing under DOT guidelines is crucial for organizations to maintain high safety margins. By identifying substance misuse, companies promote a safer community in Highland, FL.

The DOT regulations encompass various testing scenarios, ensuring comprehensive safety assessments. In Highland, Florida, abiding by these mandates is paramount to maintaining operational integrity.

FMCSA Information

In Highland, FL, the FMCSA enforces rigorous drug and alcohol testing to ensure highway safety. It's crucial for maintaining a drug-free environment in transportation.

FMCSA's regulations support a safe and compliant workplace, prioritizing the safety of drivers and passengers in Highland, Florida.

USCG Information

With USCG rules, marine employers in Highland, FL conduct testing for seafarers, ensuring a safe and secure maritime operation.

USCG requirements contribute to maintaining a vigilant and secure maritime environment in Highland, Florida.

FAA Information

Highland, FL, adheres to FAA regulations requiring aviation sector employees to undergo frequent drug and alcohol testing, vital for flight safety and security.

FAA's mandates are integral for sustaining an accident-free aviation environment in Highland, Florida.

FRA Information

The FRA ensures rail systems in Highland, FL adhere to safety-critical drug screening, enhancing operational reliability and preventing accidents.

FRA regulations bring robust public and worker safety, maintaining the infrastructure integrity of Highland, Florida.

FTA Information

FTA regulations help transit employers in Highland, FL remain compliant with drug and alcohol policies, crucial for safe public transportation.

Ensuring public transit safety in Highland, Florida, the FTA mandates regular and stringent drug testing protocols.

PHMSA Information

The PHMSA stipulated tests in Highland, FL, aim to secure pipeline and hazardous materials safety. These regulations ensure incidents of mishandling are reduced or eliminated.

Strict PHMSA checks maintain the integrity of hazardous materials transport in Highland, Florida, upholding public safety standards.

Scheduling in Highland, FL

To arrange DOT drug or alcohol testing in Highland, FL, contact us at (800) 221-4291 or use our online express scheduling. Complete the Donor Information for a seamless experience. Registration is compulsory before visiting a testing center.

Use your zip code to locate the nearest testing center in Highland. Post registration, a donor pass with the testing center details will be emailed to you. Usually, no prior appointment is needed; ensure to pay during registration.

Our SAMHSA-certified labs ensure test results are verified by our trained Medical Review Officers (MROs).

Beyond Highland, Accredited Drug Testing offers DOT services across the surrounding metropolitan areas.

Whether on the road or holidaying, our national network of testing centers means we can facilitate your test at any location, any time!

Florida Dept. of Transportation Updates

DUI Statistics in Highland County, FL

Highland County, located in the heart of Florida, has witnessed fluctuating DUI statistics in recent years. The Department of Transportation (DOT) recognizes the impact of driving under the influence (DUI) on public safety. In 2022, Highland County, FL, recorded a significant number of DUI arrests, aligning with statewide trends in Florida (FL). Efforts by local law enforcement and community initiatives seek to curb these occurrences by implementing stricter patrol measures and awareness campaigns. Highland, FL remains vigilant in addressing DUI incidents, advocating for educational programs, and promoting sober driving to reduce accidents and fatalities associated with alcohol-impaired driving in the area.

Drug-Involved Accidents in Highland County, FL

Highland County, FL, like other regions across Florida, faces challenges with drug-involved accidents. The DOT highlights drug-impaired driving as a growing concern, as it poses significant risks on roads in Highland, FL. In recent reports, Highland County has documented an increase in accidents where drug impairment was a factor. Authorities attribute this trend to the prevalence of various controlled substances. Efforts to address this include heightened enforcement, public education campaigns promoting the dangers of driving under the influence of drugs, and collaboration with healthcare providers to reduce prescription drug misuse. These initiatives are crucial for enhancing road safety and reducing drug-related accidents in the region.

Marijuana-Related Accidents in Highland County, FL

The rise of marijuana use following changes in legislation in Florida (FL) has correlated with a notable impact on traffic incidents in Highland County. The DOT in Florida recognizes the implications of marijuana-impaired driving, which has become a focal concern in Highland, FL. Recent statistics indicate that marijuana-related traffic accidents have increased, mirroring trends seen statewide. Local authorities in Highland County, FL, engage in educational outreach and legal measures to mitigate these incidents. Through awareness campaigns on the effects of driving under the influence of marijuana and collaboration with legal institutions, the community strives to enhance road safety and minimize marijuana-related accidents, reinforcing the commitment to protecting lives on Florida's roads.

Highland Drug Testing

Quickly find trusted local drug testing centers in Highland, FL — fast, convenient, and reliable every time!

Highland DOT Drug Testing

Quickly find a local DOT drug testing center in Highland, FL — fast, reliable, convenient nationwide service!

Highland DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Highland, FL.

Highland Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Highland, FL.v

Highland Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Highland, FL locations—results you can trust, every time!

Highland Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Highland, FL.

Highland Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Highland, FL.

Highland 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Highland, FL employers and individuals nationwide.

Highland 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Highland, FL workplace and personal testing needs.

Highland On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Highland, FL workplace compliant.

Highland DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Highland, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291