DOT Drug Testing - Jacksonville, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 37 testing centers in the Jacksonville area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

37 DOT Drug-Testing Centers in Jacksonville

Minutes Away, Test Today

47 W Adams St

0.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
47 W Adams St
Jacksonville, FL 32202

300 W Adams St Ste 240

0.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
300 W Adams St Ste 240
Jacksonville, FL 32202

2545 Riverside Ave Ste 3

1.24 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2545 Riverside Ave Ste 3
Jacksonville, FL 32205

1325 San Marco Blvd Ste 301

1.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1325 San Marco Blvd Ste 301
Jacksonville, FL 32207

2570 Atlantic Blvd

2.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2570 Atlantic Blvd
Jacksonville, FL 32207

2063 Oak St

2.10 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-4:00 PM • Sat: 10:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
2063 Oak St
Jacksonville, FL 32204

5212 N Pearl St

2.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5212 N Pearl St
Jacksonville, FL 32208

5685 Norwood Ave

2.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5685 Norwood Ave
Jacksonville, FL 32208

1021 Cesery Blvd

3.51 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
1021 Cesery Blvd
Jacksonville, FL 32211

3109 Spring Glen Rd Ste 302

3.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3109 Spring Glen Rd Ste 302
Jacksonville, FL 32207

4498 Hendricks Ave

3.99 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
4498 Hendricks Ave
Jacksonville, FL 32207

5960 Beach Blvd Ste 3

4.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5960 Beach Blvd Ste 3
Jacksonville, FL 32207

5960 Beach Blvd

4.21 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM (Lunch: 12:00 PM - 12:30 PM)✓ Walk-in with Electronic Donor Pass
5960 Beach Blvd
Jacksonville, FL 32207

837 Edgewood Ave N

4.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
837 Edgewood Ave N
Jacksonville, FL 32254

3901 University Blvd S Ste 203

4.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3901 University Blvd S Ste 203
Jacksonville, FL 32216

1718 Edgewood Ave N

4.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1718 Edgewood Ave N
Jacksonville, FL 32254

1610 Blanding Blvd

5.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1610 Blanding Blvd
Jacksonville, FL 32210

4933-1 University Blvd W

5.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4933-1 University Blvd W
Jacksonville, FL 32216

1912 Hamilton St Ste 103

5.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1912 Hamilton St Ste 103
Jacksonville, FL 32210

4616 San Juan Ave

5.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4616 San Juan Ave
Jacksonville, FL 32210

5964 Normandy Blvd

5.85 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
5964 Normandy Blvd
Jacksonville, FL 32205

6639 Southpoint Pkwy Ste 106

6.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6639 Southpoint Pkwy Ste 106
Jacksonville, FL 32216

5501 Roosevelt Blvd

6.35 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5501 Roosevelt Blvd
Jacksonville, FL 32244

4063 Salisbury Rd Ste 205

6.41 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4063 Salisbury Rd Ste 205
Jacksonville, FL 32216

7033 Commonwealth Ave Ste 8

6.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7033 Commonwealth Ave Ste 8
Jacksonville, FL 32220

4237 Salisbury Rd Ste 304

6.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4237 Salisbury Rd Ste 304
Jacksonville, FL 32216

6671 Hyde Grove Ave

6.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6671 Hyde Grove Ave
Jacksonville, FL 32210

1403 Dunn Ave Ste 26

6.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1403 Dunn Ave Ste 26
Jacksonville, FL 32218

1680 Dunn Ave Ste 39

6.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1680 Dunn Ave Ste 39
Jacksonville, FL 32218

1205 Monument Rd Ste 203

7.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1205 Monument Rd Ste 203
Jacksonville, FL 32225

4372 Southside Blvd, Ste 304

7.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4372 Southside Blvd, Ste 304
Jacksonville, FL 32216

231 Airline Way

7.41 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
231 Airline Way
Jacksonville, FL 32217

2032 Dunn Ave

7.48 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
2032 Dunn Ave
Jacksonville, FL 32218

1584 Normandy Village Parkway Ste 32

7.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1584 Normandy Village Parkway Ste 32
Jacksonville, FL 32221

2262 Dunn Ave Ste 2

7.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2262 Dunn Ave Ste 2
Jacksonville, FL 32218

2255 Dunn Ave

7.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2255 Dunn Ave
Jacksonville, FL 32218

7933 Baymeadows Way Ste 3

7.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7933 Baymeadows Way Ste 3
Jacksonville, FL 32256

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 37 DOT drug testing locations to serve you in and around Jacksonville, FL.

In Jacksonville, FL, Accredited Drug Testing's local sites cater to DOT Drug & Alcohol Testing, DOT Physicals, Random pool/Consortium enrollment, MIS/MVR reports, and driver qualifications, supporting employers and individual operators across all DOT modes, including FMCSA, FTA, FAA, USCG, FRA, and PHMSA, as mandated by 49 CFR Part 40. Our strategically located testing centers offer same-day services for convenience, ensuring easy access from both home and office locations.

Select your DOT service or drug/alcohol test and book at a nearby Jacksonville facility. Whether for personal, employee, or third-party testing, our efficient system simplifies arranging DOT drug testing near Jacksonville with minimal effort.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Jacksonville, Florida DOT Services

DOT Drug Testing & Services Available in Jacksonville, FL:

At Accredited Drug Testing in Jacksonville, FL, we provide robust DOT testing services, supporting local businesses and individuals with comprehensive drug and alcohol screening to meet regulatory requirements effectively and efficiently.

DOT Services in Jacksonville, FL

Understanding DOT regulations is crucial for maintaining safe and compliant workplaces in Jacksonville, Florida. These rules necessitate comprehensive drug and alcohol screening for employees, ensuring adherence to standards.

DOT screening requirements focus on the safety and security of workers and the public, with Accredited Drug Testing ensuring full regulatory compliance in Jacksonville, FL to cultivate a safe working atmosphere.

Employers in Jacksonville, Florida must implement strict adherence to DOT's drug and alcohol testing protocols, as enforced by Accredited Drug Testing, to support employee safety and legal compliance.

Jacksonville, FL regulations include random drug and alcohol tests governed by DOT, demanding vigilant employer oversight to maintain workplace safety standards and prevent infractions.

FMCSA Information

FMCSA's rules in Jacksonville, FL apply stringent DOT drug and alcohol testing to ensure roadway safety for commercial drivers and the public, enforced by Accredited Drug Testing's reliable services.

In Jacksonville, Florida, FMCSA testing regulations are vital for commercial driver's health and public safety, with compliant screening like ours facilitating adherence to strict DOT standards.

USCG Information

USCG safety standards in Jacksonville, FL command rigorous drug and alcohol tests for maritime workers, with Accredited Drug Testing ensuring thorough compliance with federal regulations.

For Jacksonville, Florida's maritime industry, USCG's testing regulations are crucial to uphold safety, with drug and alcohol testing helping maintain this essential federal compliance.

FAA Information

The FAA enforces stringent, mandatory drug and alcohol testing in Jacksonville, FL, safeguarding aviation safety. Accredited Drug Testing ensures compliance through precise testing services.

In Jacksonville, Florida, DOT FAA rules are pivotal for aviation employee safety, demanding comprehensive testing to meet the rigorous standards necessary for the industry's regulatory compliance.

FRA Information

Accredited Drug Testing complies with FRA's stringent drug and alcohol regulations in Jacksonville, ensuring rail industry safety. Our testing meets federal compliance standards for worker and public safety.

The FRA requires comprehensive safety compliance in Jacksonville, Florida, making drug and alcohol testing essential for rail workers' safety, supported by Accredited Drug Testing's expertise.

FTA Information

In Jacksonville, FL, FTA mandates enforce systematic drug and alcohol testing to promote transit safety, with Accredited Drug Testing providing compliant services to ensure operational safety.

Jacksonville, Florida upholds FTA guidelines for public transit safety, necessitating rigorous drug and alcohol testing as a key compliance measure supported by Accredited Drug Testing.

PHMSA Information

Jacksonville, FL's DOT PHMSA regulations mandate thorough drug and alcohol testing for pipeline safety workers, ensuring reliable service through Accredited Drug Testing's well-established processes.

Effective DOT PHMSA regulations in Jacksonville, Florida, require rigorous testing protocols for pipeline operations to maintain high safety standards, crucial for preventing hazardous incidents.

Scheduling in Jacksonville, FL

To arrange a DOT drug or alcohol test in Jacksonville, FL, reach our scheduling department at (800) 221-4291 or use our online express scheduling system. Register your test selection and complete the Donor Information/Registration Section before visiting the testing center.

Enter your zip code to find the nearest Jacksonville, FL testing site offering your chosen DOT drug or alcohol test. We'll email you a donor pass with the test center's address, hours, and instructions, to be presented either in printed form or on your phone. Generally, no appointment is required but ensure registration and payment beforehand.

Our laboratories hold SAMHSA certification, ensuring test results are validated by our in-house licensed physicians functioning as Medical Review Officers (MRO).

Accredited Drug Testing not only conducts DOT drug and alcohol tests in Jacksonville but also has a broad network of testing centers across the metro area and other nearby cities.

For traveling individuals or those on vacation, Accredited Drug Testing offers flexibility with facilities in cities nationwide, providing testing at your convenience, wherever, whenever required!

Florida Dept. of Transportation Updates

Jacksonville, FL DUI Statistics

In Jacksonville, Florida, DUI incidents have been a critical concern over the years due to their severe impact on road safety. According to the Florida Department of Highway Safety and Motor Vehicles, DUI-related accidents in Duval County, where Jacksonville is situated, have shown fluctuating trends over recent years. In 2020 alone, there were multiple incidents involving impaired driving, leading to numerous injuries and fatalities. Statistics indicate that alcohol-impaired driving accounts for a significant portion of road accidents in Jacksonville, FL, highlighting the need for ongoing awareness and enforcement efforts. Florida law enforcement continues to prioritize DUI checks, aiming to reduce these incidents through educational campaigns and stricter penalties for offenders.

Drug-Involved Accidents in Jacksonville, FL

Jacksonville, FL, along with the broader Duval County, experiences a notable number of traffic accidents where drugs play a significant role. Across the state of Florida, there has been a concerted effort to address the issue of drug-impaired driving due to its increasing contribution to traffic accidents. In recent years, data has reflected a concerning trend with more drivers being impaired by substances other than alcohol. This includes prescription medications and illegal drugs. Initiatives within Jacksonville and the Florida transportation sector have focused on educating the public on the dangers of driving under the influence of drugs, enhancing the efforts of law enforcement to detect and address impaired driving on Florida roads.

Marijuana-Related Accidents in Jacksonville, FL

The growing legalization of marijuana across various states within the US, including developments in Florida, has raised concerns about its potential effects on road safety in Jacksonville, FL. Although marijuana usage has been partially decriminalized in Florida for medical purposes, its impact on driving ability remains substantial. In Jacksonville, FL, marijuana-related traffic accidents are being closely monitored by local authorities. The Florida Department of Transportation has been active in studying these incidents to understand the correlation between marijuana usage and increased traffic risks. Campaigns in Jacksonville, as well as state-wide programs, strive to educate drivers on the potential impairments caused by marijuana to mitigate the rise in such incidents, aiming to promote safer road conditions throughout Florida.

Jacksonville Drug Testing

Quickly find trusted local drug testing centers in Jacksonville, FL — fast, convenient, and reliable every time!

Jacksonville DOT Drug Testing

Quickly find a local DOT drug testing center in Jacksonville, FL — fast, reliable, convenient nationwide service!

Jacksonville DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Jacksonville, FL.

Jacksonville Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Jacksonville, FL.v

Jacksonville Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Jacksonville, FL locations—results you can trust, every time!

Jacksonville Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Jacksonville, FL.

Jacksonville Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Jacksonville, FL.

Jacksonville 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Jacksonville, FL employers and individuals nationwide.

Jacksonville 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Jacksonville, FL workplace and personal testing needs.

Jacksonville On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Jacksonville, FL workplace compliant.

Jacksonville DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Jacksonville, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291