DOT Drug Testing - Kissimmee, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 35 testing centers in the Kissimmee area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

35 DOT Drug-Testing Centers in Kissimmee

Minutes Away, Test Today

820 N John Young Pkwy

0.36 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM⚡ Drug Screens: Friday: 06:30 am - 02:30 pm
820 N John Young Pkwy
Kissimmee, FL 34741

700 W Oak St

0.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
700 W Oak St
Kissimmee, FL 34741

1910 W Oak St

0.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1910 W Oak St
Kissimmee, FL 34741

413 W Oak St,

0.55 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
413 W Oak St,
Kissimmee, FL 34741

4320 W Vine St

2.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4320 W Vine St
Kissimmee, FL 34746

1325 E Vine St

2.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1325 E Vine St
Kissimmee, FL 34744

1192 Cypress Glen Circle

2.90 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1192 Cypress Glen Circle
Kissimmee, FL 34741

1414 E Osceola Pkwy

3.04 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
1414 E Osceola Pkwy
Kissimmee, FL 34744

3313 S Orange Blossom Trl, Ste B

3.17 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-2:30 PM✓ Walk-in with Electronic Donor Pass
3313 S Orange Blossom Trl, Ste B
Kissimmee, FL 34746

3010 Hunters Creek Blvd

3.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3010 Hunters Creek Blvd
Orlando, FL 32837

14050 Town Loop Blvd, Suite 105

3.90 mi
🕑 Testing Hours: Mon–Sat: 6:00 AM-2:30 PM✓ Walk-in with Electronic Donor Pass
14050 Town Loop Blvd, Suite 105
Orlando, FL 32837

2238 E Irlo Bronson Hwy,

4.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2238 E Irlo Bronson Hwy,
Kissimmee, FL 34744

317-319 Commerce Ctr Dr

6.96 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
317-319 Commerce Ctr Dr
Saint Cloud, FL 34769

12500 S Apopka Vineland Rd

7.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12500 S Apopka Vineland Rd
Orlando, FL 32836

9500 Satellite Blvd Ste 100

8.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9500 Satellite Blvd Ste 100
Orlando, FL 32837

9815 S Orange Blossom Trl

8.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9815 S Orange Blossom Trl
Orlando, FL 32837

1530 Celebration Blvd., Suite 102

9.00 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-2:30 PM • Sat: 6:00 AM-2:30 PM✓ Walk-in with Electronic Donor Pass
1530 Celebration Blvd., Suite 102
Celebration, FL 34747

9400 Turkey Lake Rd

9.45 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9400 Turkey Lake Rd
Orlando, FL 32819

7300 Sandlake Commons Blvd 205

9.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7300 Sandlake Commons Blvd 205
Orlando, FL 32819

8818 Commodity Cir Ste 41

9.58 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8818 Commodity Cir Ste 41
Orlando, FL 32819

8723 International Dr Ste 115

9.88 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8723 International Dr Ste 115
Orlando, FL 32819

8150 Chancellor Dr Ste 130

10.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8150 Chancellor Dr Ste 130
Orlando, FL 32809

2301 Sand Lake Rd

10.09 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
2301 Sand Lake Rd
Orlando, FL 32809

7751 Kingspointe Pkwy Ste 114

10.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7751 Kingspointe Pkwy Ste 114
Orlando, FL 32819

4551 Pleasant Hill Rd

10.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4551 Pleasant Hill Rd
Kissimmee, FL 34759

1941 Lazy Oaks Loop

10.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1941 Lazy Oaks Loop
Saint Cloud, FL 34771

7121 Grand National Dr

10.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7121 Grand National Dr
Orlando, FL 32819

1718 Sand Lake Rd C-106

11.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1718 Sand Lake Rd C-106
Orlando, FL 32809

6220 S Orange Blossom Trl Ste 518

11.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6220 S Orange Blossom Trl Ste 518
Orlando, FL 32809

1462 W Oak Ridge Rd

11.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1462 W Oak Ridge Rd
Orlando, FL 32809

5728 Major Blvd Ste 207

12.24 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5728 Major Blvd Ste 207
Orlando, FL 32819

1485 Legends Blvd

12.61 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1485 Legends Blvd
Champions Gate, FL 33896

6001 Vineland Rd Ste 108

12.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6001 Vineland Rd Ste 108
Orlando, FL 32819

8201 W Irlo Bronson Memorial Hwy

12.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8201 W Irlo Bronson Memorial Hwy
Kissimmee, FL 34747

4554 S Kirkman Rd

13.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4554 S Kirkman Rd
Orlando, FL 32811

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 35 DOT drug testing locations to serve you in and around Kissimmee, FL.

In Kissimmee, FL, our DOT testing centers offer comprehensive services including drug and alcohol testing, physical exams, random pool and consortium enrollment, MIS and driver reports, and more for diverse DOT modes compliant with 49 CFR Part 40 like FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Quick access and same-day services facilitate convenient testing near your location.

Choose your requisite DOT service or specific test at a preferred center. Our seamless scheduling streamlines arranging DOT tests nearby effortlessly for you or your employees.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Kissimmee, Florida DOT Services

DOT Drug Testing & Services Available in Kissimmee, FL:

In Kissimmee, FL, we provide comprehensive DOT services, ensuring quick and effective testing for compliance and safety. Our facilities accommodate a broad spectrum of testing needs with precision.

DOT Services in Kissimmee, FL

DOT regulations mandate systematic drug and alcohol screening processes to ensure safety in workplaces like Kissimmee, FL. These regulations safeguard public welfare by employing strict compliance checks.

In Kissimmee, Florida, rigorous DOT protocols are followed to maintain a secure work environment through regular assessments and screenings of employees in safety-sensitive roles.

DOT screenings executed in Kissimmee, FL, are part of a broader regulatory framework securing both employee safety and public confidence by limiting risk from impairments.

The stringent guidelines enforced under DOT regulations in Kissimmee, Florida, enhance workplace safety by reducing chances of accidents due to substance use.

FMCSA Information

FMCSA regulations in Kissimmee, FL demand rigorous drug and alcohol testing to ensure transportation safety and compliance within commercial motor vehicle operations.

In Kissimmee, Florida, FMCSA guidelines assure that drivers undergo regular screenings to maintain safety and operational standards in transportation services.

USCG Information

USCG rules ensure marine safety in Kissimmee, FL by holding maritime employees to stringent drug and alcohol testing standards.

In Kissimmee, Florida, USCG regulations enforce comprehensive testing for the safe and compliant operation of maritime activities.

FAA Information

Kissimmee, FL upholds FAA mandates requiring aviation employees to undergo drug testing, reinforcing safety and regulatory compliance in air transport.

FAA's strict drug testing protocols in Kissimmee, Florida ensure aviation safety and reliability through comprehensive employee screenings.

FRA Information

FRA imposes strict drug and alcohol testing in railways across Kissimmee, FL, ensuring adherence to safety standards through constant screenings.

In Kissimmee, Florida railroad employees are subject to rigorous FRA testing, maintaining safety and operational integrity in rail services.

FTA Information

Kissimmee, FL follows FTA mandates closely, ensuring regular testing of public transportation employees for optimal safety and compliance.

FTA rules in Kissimmee, Florida require rigorous drug and alcohol tests, promoting public transport safety and reliability.

PHMSA Information

PHMSA regulations govern pipeline testing in Kissimmee, FL, demanding strict adherence to drug and alcohol testing for safety and operational integrity.

Kissimmee, Florida follows PHMSA standards for drug screenings, ensuring no compromises on safety within hazardous material transport.

Scheduling in Kissimmee, FL

To schedule a DOT test in Kissimmee, FL, contact our team at (800) 221-4291 or conveniently book online via our express registration. Ensure registration completion before visiting the center.

Enter your zip code to identify the nearest Kissimmee DOT testing site. A donor pass, detailing address and instructions, will be emailed. Carry it or display it on a smartphone at the test site; appointments are generally unnecessary but complete registration and payment are required upfront.

Our SAMHSA-certified labs ensure reliable results confirmed by our licensed Medical Review Officers.

Beyond Kissimmee, Accredited Drug Testing offers centers across the metro area and adjacent cities, granting easy access for comprehensive DOT services.

Traveling? No matter the location, Accredited Drug Testing accommodates your testing needs nationwide at any time, securing convenience across all venues.

Florida Dept. of Transportation Updates

DUI Statistics in Kissimmee, FL

In Kissimmee, located in Osceola County, Florida, DUI incidents have been a major concern for local law enforcement and the DOT. The state of Florida has stringent laws regarding driving under the influence, with serious penalties for those caught. In recent years, there has been a mix of education and enforcement efforts aimed at reducing these numbers. High-visibility enforcement campaigns, along with public awareness programs, have been actively implemented to address this issue. Florida’s DOT has worked closely with law enforcement agencies in Kissimmee and broader Osceola County to ensure roads remain safe. Throughout these initiatives, a noticeable decline in the number of DUI-related arrests and accidents has been observed, though continuous efforts are necessary to maintain and further this trend.

Drug-Involved Accidents in Kissimmee, FL

Kissimmee, FL, and its encompassing area of Osceola County, have faced challenges related to drug-involved traffic accidents. The Florida Department of Transportation has been vigilant in tracking and addressing incidents where drivers were under the influence of substances other than alcohol. Programs focusing on the risks of driving while impaired by drugs have been rolled out across the state, highlighting statistics and real-life consequences of such actions. Collaborative efforts among local authorities in Kissimmee have aimed to educate the public about the risks and penalties associated with drug-impaired driving. Despite these measures, drug-related incidents continue to be a concern, demanding ongoing preventive measures and robust enforcement to tackle this issue effectively.

Marijuana-Related Accidents in Kissimmee, FL

With the evolving legal landscape surrounding marijuana in Florida, Kissimmee, situated in Osceola County, experiences its share of challenges with marijuana-related traffic accidents. The state's DOT has been closely monitoring the eventual impact of marijuana legalization on road safety. Public safety campaigns have been instrumental in educating Kissimmee residents about the effects of driving under the influence of marijuana, alongside traditional law enforcement efforts. The DOT, alongside local agencies, has worked tirelessly to ensure that drivers understand the dangers associated with drug-impaired driving. Statistics gathered from Osceola County reflect an ongoing concern for marijuana's impact on traffic safety, necessitating the relentless efforts of education and enforcements to manage this emerging issue effectively.

Kissimmee Drug Testing

Quickly find trusted local drug testing centers in Kissimmee, FL — fast, convenient, and reliable every time!

Kissimmee DOT Drug Testing

Quickly find a local DOT drug testing center in Kissimmee, FL — fast, reliable, convenient nationwide service!

Kissimmee DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Kissimmee, FL.

Kissimmee Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Kissimmee, FL.v

Kissimmee Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Kissimmee, FL locations—results you can trust, every time!

Kissimmee Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Kissimmee, FL.

Kissimmee Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Kissimmee, FL.

Kissimmee 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Kissimmee, FL employers and individuals nationwide.

Kissimmee 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Kissimmee, FL workplace and personal testing needs.

Kissimmee On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Kissimmee, FL workplace compliant.

Kissimmee DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Kissimmee, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291