DOT Drug Testing - Orlando, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 29 testing centers in the Orlando area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

29 DOT Drug-Testing Centers in Orlando

Minutes Away, Test Today

601 E Colonial Dr

1.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
601 E Colonial Dr
Orlando, FL 32803

2323 S Orange Ave Ste A

1.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2323 S Orange Ave Ste A
Orlando, FL 32806

701 E Michigan St

1.88 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
701 E Michigan St
Orlando, FL 32806

709 E Michigan St

1.88 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
709 E Michigan St
Orlando, FL 32806

813 E Michigan St

1.89 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM • Sat: 8:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
813 E Michigan St
Orlando, FL 32806

630 N Bumby Ave

1.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
630 N Bumby Ave
Orlando, FL 32803

3615 S Orange Ave

2.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3615 S Orange Ave
Orlando, FL 32806

Bldg 49635 - Rm 1219

2.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Bldg 49635 - Rm 1219
Orlando, FL 32899

2314 N Orange Blossom Trl

2.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2314 N Orange Blossom Trl
Orlando, FL 32804

4401 S Orange Ave Ste 110

2.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4401 S Orange Ave Ste 110
Orlando, FL 32806

324 E Par St.., Ste 100,

3.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
324 E Par St.., Ste 100,
Orlando, FL 32804

509 S Semoran Blvd

4.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
509 S Semoran Blvd
Orlando, FL 32807

36 S Semoran Blvd Ste B

4.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
36 S Semoran Blvd Ste B
Orlando, FL 32807

483 N Semoran Blvd Suite 103

4.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
483 N Semoran Blvd Suite 103
Winter Park, FL 32792

1338 Pleasant Oak Ln

4.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1338 Pleasant Oak Ln
Orlando, FL 32804

611 N Wymore Rd Ste 220

4.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
611 N Wymore Rd Ste 220
Winter Park, FL 32789

501 N Orlando Ave Ste 227

4.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
501 N Orlando Ave Ste 227
Winter Park, FL 32789

2540 Lee Rd

4.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2540 Lee Rd
Winter Park, FL 32789

1850 Lee Rd Ste 216

4.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1850 Lee Rd Ste 216
Winter Park, FL 32789

1850 Lee Rd Ste 300

4.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1850 Lee Rd Ste 300
Winter Park, FL 32789

1462 W Oak Ridge Rd

4.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1462 W Oak Ridge Rd
Orlando, FL 32809

5021 Eggleston Ave Ste A

4.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5021 Eggleston Ave Ste A
Orlando, FL 32804

4780 N Orange Blossom Trl

4.87 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
4780 N Orange Blossom Trl
Orlando, FL 32810

6220 S Orange Blossom Trl Ste 518

5.05 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6220 S Orange Blossom Trl Ste 518
Orlando, FL 32809

2555 S Kirkman Rd

5.11 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
2555 S Kirkman Rd
Orlando, FL 32811

1718 Sand Lake Rd C-106

5.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1718 Sand Lake Rd C-106
Orlando, FL 32809

1603 S Hiawassee Rd, Suite 120

5.64 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-2:30 PM • Sat: 6:30 AM-12:30 PM✓ Walk-in with Electronic Donor Pass
1603 S Hiawassee Rd, Suite 120
Orlando, FL 32835

5425 S Semoran Blvd

5.68 mi
🕑 Facility: Mon–Fri: 06:30 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 06:30 AM - 03:00 PM
5425 S Semoran Blvd
Orlando, FL 32822

4554 S Kirkman Rd

5.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4554 S Kirkman Rd
Orlando, FL 32811

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 29 DOT drug testing locations to serve you in and around Orlando, FL.

Our DOT Testing Centers in Orlando, FL, and nearby areas provide essential services such as DOT Drug and Alcohol Testing, DOT Physicals, Random Pool and Consortium Enrollment, among others. These services cater to Employers, Independent Operators, and various DOT modes like FMCSA, FTA, FAA, USCG, FRA, and PHMSA, ensuring compliance with 49 CFR Part 40. Our locations are conveniently situated for easy access from both home and office, with most cases offering same-day service.

Choose the required DOT test or service and find a nearby facility for easy testing—ideal for individuals, employees, or others. Our hassle-free procedure allows you to arrange DOT drug testing in Orlando seamlessly.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Orlando, Florida DOT Services

DOT Drug Testing & Services Available in Orlando, FL:

In Orlando, FL, Accredited Drug Testing offers a comprehensive array of DOT services. From drug and alcohol testing to DOT physicals, we are equipped to meet all your regulatory compliance needs efficiently and reliably.

DOT Services in Orlando, FL

DOT regulations enforce stringent drug and alcohol screening to uphold employee safety in Orlando, FL. These guidelines are crucial for maintaining safety across all transportation sectors.

In Orlando, Florida, the regulations ensure that transportation workers are routinely screened, reducing risks and hazards in public transport systems, enhancing passenger protection.

Employers in Orlando, FL must abide by these standards, implementing mandatory tests to comply with federal law, thus fostering a safer work environment.

These rules are designed to detect substance abuse early, preventing potential accidents and maintaining public trust in transportation services throughout Orlando, Florida.

FMCSA Information

The FMCSA mandates regular drug and alcohol tests for commercial drivers in Orlando, FL, ensuring road safety and compliance with national standards.

Drivers in Orlando, Florida, must pass stringent testing to retain their commercial licenses, reinforcing public trust in the transport system.

FMCSA regulations in Orlando enforce zero tolerance for substance abuse, upholding a secure commercial driving environment.

USCG Information

For maritime operations in Orlando, FL, USCG drug and alcohol tests are critical in preserving safety on waterways.

USCG regulations in Orlando ensure that vessel operators are substance-free, safeguarding both crew and passengers on marine trips.

By enforcing these rules in Orlando, the USCG ensures maritime activities are performed safely and efficiently, boosting public assurance.

FAA Information

In Orlando, FL, FAA drug and alcohol testing rules apply to aviation personnel, guaranteeing the highest safety standards in air transport.

These FAA regulations in Orlando ensure that pilots and crew are sober, promoting safe air travel and public confidence.

Testing under FAA guidelines is crucial for Orlando's aviation industry, maintaining disciplined and safe airline operations.

FRA Information

FRA guidelines necessitate routine drug and alcohol testing for rail workers in Orlando, FL, enhancing safety in the rail transport sector.

In Orlando, FRA testing ensures that rail personnel comply with safety standards, reducing the likelihood of rail-related incidents.

Adhering to FRA rules in Orlando guarantees that railways operate without the influence of prohibited substances, securing public transport.

FTA Information

FTA drug and alcohol rules apply to public transit employees in Orlando, FL, maintaining high safety levels in mass transit systems.

Testing under FTA standards ensures Orlando's public transit staff is sober, ensuring passenger safety and reliability in services.

Orlando, Florida's commitment to FTA guidelines minimizes risks associated with substance impairment in public transportation.

PHMSA Information

PHMSA regulations require periodic screening for transport workers in hazardous material sectors in Orlando, FL, ensuring safety and compliance.

Orlando, Florida-based workers involved in material handling must adhere to PHMSA rules to prevent dangers associated with hazardous substances.

By enforcing strict testing, PHMSA in Orlando helps protect both employees and the public from potential mishaps involving hazardous materials.

Scheduling in Orlando, FL

For scheduling any DOT drug or alcohol test in Orlando, FL, contact our local desk at (800) 221-4291 or utilize our online express system by selecting your test and completing the necessary registration details. Register before visiting the testing site.

Your input zip code identifies the nearest Orlando testing center providing your chosen tests. Post-registration, you'll receive an email with your donor pass, center details, and instructions—carry it physically or digitally. No appointments are required for most scenarios, but donor details and test payment must be completed during sign-up.

Our labs boast SAMHSA certification, with results vetted by in-house MRO physicians.

Accredited Drug Testing extends its DOT drug and alcohol testing services beyond Orlando, FL, with numerous centers in nearby cities.

Whether traveling or on break, enjoy peace of mind knowing Accredited Drug Testing operates testing locations across the nation, ready to schedule your test anytime, anywhere.

Florida Dept. of Transportation Updates

Orlando, FL DUI Statistics

Orlando, located in Orange County, Florida, has been addressing the challenges posed by DUI incidents. The Florida Department of Transportation (FDOT) reported that DUI-related crashes in Orlando and surrounding areas have consistently raised concerns for public safety. In recent years, Orlando has seen a concerted effort by local law enforcement to reduce DUI incidents through increased checkpoints and awareness campaigns. Orange County ranks among the top counties in Florida for DUI arrests, largely due to its populous nature and the presence of a vibrant nightlife. Efforts to curb these incidents include educational initiatives targeting both residents and tourists about the dangers of driving under the influence in FL.

Orlando, FL Drug Involved Accidents

Drug-involved accidents in Orlando, FL, have been a critical focus for the city's Department of Transportation. Recent data from the FDOT indicates that drug-related crashes have shown a noticeable presence in Orange County. These accidents often involve a variety of substances, with law enforcement agencies working diligently to address the complexities involved. Orlando's strategic location as a travel hub contributes to its exposure to drug trafficking routes, which can influence the rates of drug-impaired driving. Local campaigns strive to educate the public on the repercussions of drugged driving, aiming to reduce the incidence rate through focused enforcement and education initiatives statewide in Florida.

Orlando, FL Marijuana Related Accidents

With the increasing legalization and social acceptance of marijuana, Orlando, FL, has observed a trend in marijuana-related traffic incidents. The FDOT has noticed that marijuana-impaired drivers present unique challenges for traffic safety in Orange County. Although these accidents represent a smaller fraction of overall traffic incidents, the Department of Transportation in Florida emphasizes the need for ongoing education and research into the effects of THC on driving abilities. Orlando's law enforcement continues to receive training to better detect and handle marijuana-impaired driving cases, contributing to a comprehensive strategy aimed at ensuring safer roads throughout Florida as marijuana use becomes more widespread.

Orlando Drug Testing

Quickly find trusted local drug testing centers in Orlando, FL — fast, convenient, and reliable every time!

Orlando DOT Drug Testing

Quickly find a local DOT drug testing center in Orlando, FL — fast, reliable, convenient nationwide service!

Orlando DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Orlando, FL.

Orlando Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Orlando, FL.v

Orlando Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Orlando, FL locations—results you can trust, every time!

Orlando Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Orlando, FL.

Orlando Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Orlando, FL.

Orlando 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Orlando, FL employers and individuals nationwide.

Orlando 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Orlando, FL workplace and personal testing needs.

Orlando On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Orlando, FL workplace compliant.

Orlando DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Orlando, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291