DOT Drug Testing - Saint James City, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 37 testing centers in the Saint James City area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

37 DOT Drug-Testing Centers in Saint James City

Minutes Away, Test Today

1708 Cape Coral Pkwy W Ste 2 # 2

6.13 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1708 Cape Coral Pkwy W Ste 2 # 2
Cape Coral, FL 33914

2612 Santa Barbara Blvd #11

9.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2612 Santa Barbara Blvd #11
Cape Coral, FL 33914

On-site Only

9.90 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
On-site Only
Cape Coral, FL 33904

16261 Bass Rd. Ste 101

10.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
16261 Bass Rd. Ste 101
Fort Myers, FL 33908

3412 Sw 3rd Ter

10.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3412 Sw 3rd Ter
Cape Coral, FL 33991

8931 Conference Dr Ste 3

11.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8931 Conference Dr Ste 3
Fort Myers, FL 33919

1431 Se 10th St A-1

12.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1431 Se 10th St A-1
Cape Coral, FL 33990

6202 Presidential Ct Ste A

12.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6202 Presidential Ct Ste A
Fort Myers, FL 33919

13401 Summerlin Rd Ste 9

13.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
13401 Summerlin Rd Ste 9
Fort Myers, FL 33919

12995 S Cleveland Ave Ste 184

13.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12995 S Cleveland Ave Ste 184
Fort Myers, FL 33907

5172 Mason Corbin Ct Ste 2

13.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5172 Mason Corbin Ct Ste 2
Fort Myers, FL 33907

1635 Medical Ln,

14.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1635 Medical Ln,
Fort Myers, FL 33907

428 Del Prado Blvd N Ste 103

14.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
428 Del Prado Blvd N Ste 103
Cape Coral, FL 33909

14131 Metropolis Ave Ste 102

14.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14131 Metropolis Ave Ste 102
Fort Myers, FL 33912

4771 S Cleveland Ave

14.34 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-7:00 PM✓ Walk-in with Electronic Donor Pass
4771 S Cleveland Ave
Fort Myers, FL 33907

13681 Doctors Way

14.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
13681 Doctors Way
Fort Myers, FL 33912

1682 Ne Pine Island Rd

14.76 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-7:00 PM✓ Walk-in with Electronic Donor Pass
1682 Ne Pine Island Rd
Cape Coral, FL 33909

4350 Fowler St Ste 2

15.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4350 Fowler St Ste 2
Fort Myers, FL 33901

2665 Cleveland Ave

15.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2665 Cleveland Ave
Fort Myers, FL 33901

10201 Arcos Ave Ste 105

18.61 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10201 Arcos Ave Ste 105
Estero, FL 33928

10020 Coconut Rd Ste 124

19.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10020 Coconut Rd Ste 124
Bonita Springs, FL 34135

9530 Bonita Bch Rd Se Ste 103

20.96 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9530 Bonita Bch Rd Se Ste 103
Bonita Springs, FL 34135

9550 Bonita Beach Rd, Unit #107

20.98 mi
🕑 Testing Hours: Mon–Fri: 11:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
9550 Bonita Beach Rd, Unit #107
Bonita Springs, FL 34135

2700 Immokalee Rd Ste 23

24.84 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2700 Immokalee Rd Ste 23
Naples, FL 34110

3400 Lee Blvd, Ste 107

25.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3400 Lee Blvd, Ste 107
Lehigh Acres, FL 33971

3020 Lee Blvd Ste 8

26.13 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3020 Lee Blvd Ste 8
Lehigh Acres, FL 33971

2718 Lee Blvd Ste B

26.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2718 Lee Blvd Ste B
Lehigh Acres, FL 33971

3785 Airport Pulling Rd N Ste B1

28.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3785 Airport Pulling Rd N Ste B1
Naples, FL 34105

720 Goodlette Rd N Ste 500

29.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
720 Goodlette Rd N Ste 500
Naples, FL 34102

661 Goodlette Rd N Ste 108

29.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
661 Goodlette Rd N Ste 108
Naples, FL 34102

311 Tamiami Trl N Ste 101

29.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
311 Tamiami Trl N Ste 101
Naples, FL 34102

311 Tamiami Trl N Ste 110

29.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
311 Tamiami Trl N Ste 110
Naples, FL 34102

311 Tamiami Trl N Ste 310

29.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
311 Tamiami Trl N Ste 310
Naples, FL 34102

809 E Marion Ave

30.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
809 E Marion Ave
Punta Gorda, FL 33950

4161 Tamiami Trl,

32.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4161 Tamiami Trl,
Charlotte Harbor, FL 33952

1715 Heritage Trl Ste 201&202

32.77 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1715 Heritage Trl Ste 201&202
Naples, FL 34112

6400 Davis Blvd Ste 103

32.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6400 Davis Blvd Ste 103
Naples, FL 34104

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 37 DOT drug testing locations to serve you in and around Saint James City, FL.

Our Saint James City, FL DOT testing centers offer comprehensive services including DOT drug tests, alcohol tests, physicals, MIS reports, and driver qualification reports. We're equipped for all DOT modes such as FMCSA, FAA, USCG, and more, ensuring compliance with 49 CFR Part 40. Access services near home or work, often with same-day availability.

Select the necessary DOT service or test, and connect with a nearby location. Our efficient system makes it simple to organize your DOT drug testing in Saint James City, whether for yourself, an employee, or another individual.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Saint James City, Florida DOT Services

DOT Drug Testing & Services Available in Saint James City, FL:

Our DOT services in Saint James City, FL are designed for maximum efficiency, ensuring all relevant testing requirements are handled smoothly and effectively, enhancing safety compliance.

DOT Services in Saint James City, FL

DOT regulations mandate regular drug and alcohol testing to safeguard the workforce in Saint James City, Florida, ensuring operational safety and compliance across transport sectors.

Employers in Saint James City, FL must adhere to stringent testing protocols, fostering a safe environment for both employees and the community at large.

Testing helps mitigate risks, maintaining integrity in operations while ensuring readiness for any safety audits or regulatory checks in Saint James City, Florida.

Comprehensive screening promotes workplace safety, benefiting employee well-being and enhancing public trust in Saint James City, FL's transportation systems.

FMCSA Information

In Saint James City, FL, FMCSA regulations ensure all commercial drivers receive regular drug screenings to promote highway safety and reduce accident risks.

Conducting FMCSA drug and alcohol tests complies with federal mandates and secures a safe working environment in Saint James City, Florida.

USCG Information

USCG testing guidelines in Saint James City, FL ensure maritime employees are fit for duty, safeguarding nautical operations and preventing potential incidents.

Testing under USCG norms in Saint James City, Florida, protects both marine workers and the environment.

FAA Information

FAA regulations require routine drug and alcohol screenings for aviation personnel in Saint James City, FL, critical for maintaining air travel safety standards.

Testing procedures in Saint James City, FL ensure compliance with FAA safety protocols, essential for aviation professionals.

FRA Information

The FRA drug testing rules in Saint James City, FL ensure railroad industry workers are substance-free, protecting key infrastructure components.

Regular screenings in Saint James City, FL adhere to FRA standards, critical for railway operational safety.

FTA Information

FTA regulations in Saint James City, FL demand thorough drug and alcohol testing for public transit workforce, enhancing commuter safety.

Saint James City, FL implements FTA rules to oversee transit safety compliance via systematic testing.

PHMSA Information

PHMSA's drug testing in Saint James City, FL targets pipeline workers to maintain safety in transporting hazardous materials, ensuring operational safety and regulatory adherence.

Consistent testing in Saint James City, Florida assists in hazard prevention, aligning with PHMSA's safety goals.

Scheduling in Saint James City, FL

For scheduling any DOT drug or alcohol screening in Saint James City, FL, contact our scheduling at (800) 221-4291. Opt to book online, using our express system, by choosing your test and finishing the Registration Section before visiting the testing center.

Upon entering your zip code, you'll receive details about the closest Saint James City, FL facility capable of your selected test. Registration provides essential information via email to present during your visit. Typically, no appointment is needed, but ensure to complete registration and payment.

Our labs hold SAMHSA certification, with all results verified by certified Medical Review Officers (MRO) for reliability.

Beyond Saint James City, FL, Accredited Drug Testing accommodates the wider metro area and nearby cities with additional facilities.

Whether traveling or on vacation, Accredited Drug Testing manages scheduling across numerous nationwide centers, offering flexibility and convenience.

Florida Dept. of Transportation Updates

Lee County, FL DUI Statistics

Driving Under the Influence (DUI) remains a critical concern in Lee County, FL, where Saint James City is located. Lee County, as part of the larger Florida state efforts, has seen significant progress in curbing DUI incidents through stringent enforcement and public awareness campaigns. In recent years, the Department of Transportation (DOT) of Florida reported a decrease in DUI arrests and accidents due to enhanced sobriety checkpoints and community education initiatives. Nevertheless, DUI-related incidents continue to pose challenges, particularly during holidays and weekends, when alcohol consumption is at its peak. Lee County's law enforcement agencies persistently coordinate with the Florida DOT to deploy targeted measures aiming to deter impaired driving and ensure road safety.

Drug-Involved Accidents in Lee County, FL

In Lee County, FL, which encompasses Saint James City, drug-involved accidents are a growing concern addressed rigorously by local authorities and the Florida Department of Transportation (FLDOT). The county aligns with Florida’s statewide strategies to reduce such incidents through aggressive public safety campaigns and inter-agency collaborations. Recent data indicate that prescription medications and illicit substances increasingly contribute to traffic incidents within the county. Efforts focus on education, promoting the dangers of drug-impaired driving, and implementing road policing tactics, such as drug recognition expert programs, which are critical in abating the trend. Continuous support from the community and innovative enforcement methodologies remain pivotal in tackling this issue within Lee County.

Marijuana-related Accidents in Lee County, FL

Lee County, FL faces challenges with marijuana-related accidents, reflecting broader trends observed across Florida. Since changes in marijuana legislation, the Florida Department of Transportation (FDOT) and local Lee County authorities have ramped up efforts to manage related road safety concerns. Data reveal a nuanced increase in marijuana presence in accident reports, warranting focused initiatives like enhanced driver education and officer training programs. These measures aim to equip law enforcement with skills to identify marijuana impairment reliably. The county continuously engages in comprehensive campaigns to inform the public about the risks of driving under the influence of marijuana, highlighting its commitment to maintaining road safety despite evolving legislative contexts surrounding cannabis use in Florida.

Saint James City Drug Testing

Quickly find trusted local drug testing centers in Saint James City, FL — fast, convenient, and reliable every time!

Saint James City DOT Drug Testing

Quickly find a local DOT drug testing center in Saint James City, FL — fast, reliable, convenient nationwide service!

Saint James City DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Saint James City, FL.

Saint James City Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Saint James City, FL.v

Saint James City Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Saint James City, FL locations—results you can trust, every time!

Saint James City Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Saint James City, FL.

Saint James City Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Saint James City, FL.

Saint James City 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Saint James City, FL employers and individuals nationwide.

Saint James City 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Saint James City, FL workplace and personal testing needs.

Saint James City On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Saint James City, FL workplace compliant.

Saint James City DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Saint James City, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291