DOT Drug Testing - Alton, IL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 35 testing centers in the Alton area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

35 DOT Drug-Testing Centers in Alton

Minutes Away, Test Today

237b E Center Dr,

2.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
237b E Center Dr,
Alton, IL 62002

108b Northport Dr Alton Med, Ctr. Lower Level

4.58 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
108b Northport Dr Alton Med, Ctr. Lower Level
Alton, IL 62002

325 Madison Ave

5.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
325 Madison Ave
Wood River, IL 62095

900 S Central Ave

6.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
900 S Central Ave
Roxanna, IL 62084

10716 New Halls Ferry Rd,

8.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10716 New Halls Ferry Rd,
Florissant, MO 63033

2880 Netherton, Ste 101

9.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2880 Netherton, Ste 101
Saint Louis, MO 63136

3316 W Chain Of Rocks Rd Ste 1

9.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3316 W Chain Of Rocks Rd Ste 1
Granite City, IL 62040

4700 Nameoki Rd

10.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4700 Nameoki Rd
Granite City, IL 62040

3701 Nameoki Rd Ste E

11.69 mi
🕑 Testing Hours: M-Th 9:00 AM-5:00 PM • F 9:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
3701 Nameoki Rd Ste E
Granite City, IL 62040

119 Church St Ste 223

11.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
119 Church St Ste 223
Ferguson, MO 63135

State Highway 76 & State Highway Lower Level Claybough Plaza Ma

11.96 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
State Highway 76 & State Highway Lower Level Claybough Plaza Ma
Branson West, MO 65737

463 Lynn Haven Ln

11.99 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
463 Lynn Haven Ln
Hazelwood, MO 63042

637 Dunn Rd Ste 155

12.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
637 Dunn Rd Ste 155
Hazelwood, MO 63042

8340 N Broadway

12.50 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
8340 N Broadway
Saint Louis, MO 63147

637 Dunn Rd Ste 135

12.53 mi
🕑 Facility: Mon–Fri: 07:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 04:00 PM
637 Dunn Rd Ste 135
Hazelwood, MO 63042

637 Dunn Rd Ste 100

12.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
637 Dunn Rd Ste 100
Hazelwood, MO 63042

7220 N Lindbergh Blvd #ste 250-60 Cargo Bay Bldg

13.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7220 N Lindbergh Blvd #ste 250-60 Cargo Bay Bldg
Hazelwood, MO 63042

1181 S State Route 157

13.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1181 S State Route 157
Edwardsville, IL 62025

102 Rottingham, Ste 2

13.24 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
102 Rottingham, Ste 2
Edwardsville, IL 62025

2100 Madison Ave

13.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2100 Madison Ave
Granite City, IL 62040

2044 Madison Ave Ste G-4

13.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2044 Madison Ave Ste G-4
Granite City, IL 62040

2044 Madison Ave Ste G1

13.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2044 Madison Ave Ste G1
Granite City, IL 62040

3440 Depaul Lane Ste. 208,

16.38 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3440 Depaul Lane Ste. 208,
Bridgeton, MO 63044

2023 Vadalebene Dr, Suite 150

16.47 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2023 Vadalebene Dr, Suite 150
Maryville, IL 62062

3446 Mckelvey Rd

16.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3446 Mckelvey Rd
Bridgeton, MO 63044

2136 Vadalabene Dr Ste A,

16.62 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-2:30 PM • Sat: 9:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
2136 Vadalabene Dr Ste A,
Maryville, IL 62062

400 Maple Summit Rd

17.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
400 Maple Summit Rd
Jerseyville, IL 62052

50 Monroe St

17.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
50 Monroe St
Saint Charles, MO 63301

40 N Kingshighway,

17.72 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-2:30 PM • Sat: 9:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
40 N Kingshighway,
Saint Louis, MO 63108

1720 Olive St

17.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1720 Olive St
Saint Louis, MO 63103

400 N Tucker Blvd

17.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
400 N Tucker Blvd
Saint Louis, MO 63101

3100 Market St

18.08 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
3100 Market St
Saint Louis, MO 63103

916 Olive Street, Unit 2

18.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
916 Olive Street, Unit 2
Saint Louis, MO 63101

400 Fountain Lakes Blvd

18.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
400 Fountain Lakes Blvd
St Charles, MO 63301

225 S Meramec Ave Ste 305

18.80 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
225 S Meramec Ave Ste 305
Clayton, MO 63105

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 35 DOT drug testing locations to serve you in and around Alton, IL.

Accredited Drug Testing offers a range of DOT services including drug tests, alcohol tests, physicals, random pool enrollment, and compliance assistance for all DOT modes in Alton, IL. Our facilities serve employers, single operators, and entities needing to align with 49 CFR Part 40, covering FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Nearby locations provide quick access, often offering same-day service to fit your schedule, whether at home or the office.

Choose your required DOT service or test and find a nearby testing site easily. Services are available for both individuals and employees. Our efficient process allows seamless scheduling for all your DOT drug and alcohol testing needs in Alton.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Alton, Illinois DOT Services

DOT Drug Testing & Services Available in Alton, IL:

Accredited Drug Testing provides extensive DOT testing services in Alton, IL, focusing on compliance, reliability, and ease. Whether for personal needs or organizational compliance, our solutions are tailored to meet specific requirements efficiently, supporting all DOT modes in the region.

DOT Services in Alton, IL

DOT regulations in Alton, Illinois are established to ensure safety and competence in personnel handling transport operations. All operators, employers, and employees must undergo rigorous drug and alcohol screenings that align with federal mandates to uphold public safety and meet compliance under DOT standards.

In Alton, IL, companies must adhere to specific procedures for drug testing established by the DOT to monitor and enhance employee safety. The organization enforces strict rules and updates its methodologies to address emerging challenges effectively.

Accredited Drug Testing not only follows current guidelines but also contributes to higher safety measures by offering educational resources and support for employers and employees in Alton, Illinois to comply with DOT regulations sustainably.

Employers in Alton, IL often utilize our company’s comprehensive suite of services, from random tests to post-accident screenings, as part of their commitment to promoting a safe working environment.

FMCSA Information

In Alton, IL, DOT FMCSA rules are designed to maintain the safety of commercial motor vehicle operations through regular drug and alcohol testing ensuring driver compliance and public safety.

Accredited Drug Testing in Alton, Illinois facilitates compliance with FMCSA mandates, ensuring drivers undergo necessary screenings as part of federal safety requisites.

USCG Information

In Alton, IL, USCG regulations mandate drug and alcohol screenings to uphold maritime safety standards, ensuring crew readiness and operational safety.

Accredited Drug Testing supports compliance with these rules, facilitating necessary screenings for maritime workers in Alton, Illinois.

FAA Information

FAA guidelines require drug and alcohol testing for aviation personnel in Alton, IL to ensure air transport safety and compliance with federal aviation standards.

Accredited Drug Testing’s Alton, Illinois centers provide tailored FAA testing services for aviation workers to maintain safety standards.

FRA Information

FRA mandates drug and alcohol testing in Alton, IL for rail industry employees to ensure compliance with safety standards, preventing accidents and promoting worker health.

Alton, Illinois residents relying on rail safety can benefit from Accredited Drug Testing’s services ensuring FRA compliance through thorough screenings.

FTA Information

Accredited Drug Testing in Alton, IL adheres to FTA rules requiring regular drug and alcohol tests for transit employees, promoting public and passenger safety.

Testing ensures Alton, Illinois’ transit operations remain safe and compliant, contributing to the community's overall safety and reliability.

PHMSA Information

DOT PHMSA rules in Alton, IL involve drug and alcohol testing to ensure that individuals involved in pipeline transportation operations maintain adherence to safety protocols.

Accredited Drug Testing helps entities in Alton, Illinois align with PHMSA requirements, sustaining operational reliability and safety adherence.

Scheduling in Alton, IL

To arrange a DOT drug or alcohol test in Alton, IL, contact us at (800) 221-4291 or use our online system for express scheduling. Select your test and complete the registration to proceed. Registration is required before visiting the testing site.

Input the ZIP code to locate the nearest testing center in Alton, Illinois. A donor pass with test details will be emailed to you, which you should carry or have accessible on your smartphone for the testing location. Most tests don't require an appointment, but registration and payment are required upfront.

Our laboratories hold SAMHSA certification and our licensed physicians verify results, serving as Medical Review Officers (MRO).

Alongside Alton, IL services, our facilities are spread across the metro area and beyond.

Accredited Drug Testing ensures accessibility with nationwide centers, supporting your testing needs anytime, anywhere.

Illinois Dept. of Transportation Updates

DUI Statistics in Alton, IL

The city of Alton, located in Madison County, Illinois, is not immune to the issues surrounding driving under the influence (DUI). According to recent data from the Illinois Department of Transportation (IDOT), DUI incidents have shown a fluctuating pattern over the last few years. For instance, in the year 2022, Alton recorded a notable number of DUI arrests, highlighting the ongoing challenges of addressing impaired driving. The state of Illinois has implemented strict laws and community awareness programs to tackle this issue, and Alton’s local authorities have been actively engaging in campaigns to reduce DUI occurrences and enhance road safety for all its residents.

Drug Involved Accidents in Alton, IL

Madison County, Illinois, where Alton is a major city, faces significant challenges regarding drug-involved vehicular accidents. Data from the Illinois Department of Transportation showcases that accidents involving drug impairment have been a major concern in recent years. In 2021 alone, a considerable percentage of traffic accidents in and around Alton were linked to drug use, prompting local and state authorities to intensify their efforts in curbing such incidents. Enhanced training for law enforcement and increased public awareness campaigns in Illinois are pivotal strategies being employed to mitigate the risks associated with drug-impaired driving.

Marijuana Related Accidents in Alton, IL

Since the legalization of recreational marijuana in Illinois, cities like Alton have been closely monitoring its impact on road safety. In the past year, Madison County has reported an increase in marijuana-related accidents, reflecting a broader statewide trend. The Illinois Department of Transportation is working with local agencies in Alton to gather comprehensive data on these incidents to develop effective countermeasures. Educational programs focusing on the dangers of driving under the influence of marijuana are being implemented to address this growing concern, ensuring that Alton’s roads remain safe for all residents.

Alton Drug Testing

Quickly find trusted local drug testing centers in Alton, IL — fast, convenient, and reliable every time!

Alton DOT Drug Testing

Quickly find a local DOT drug testing center in Alton, IL — fast, reliable, convenient nationwide service!

Alton DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Alton, IL.

Alton Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Alton, IL.v

Alton Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Alton, IL locations—results you can trust, every time!

Alton Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Alton, IL.

Alton Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Alton, IL.

Alton 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Alton, IL employers and individuals nationwide.

Alton 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Alton, IL workplace and personal testing needs.

Alton On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Alton, IL workplace compliant.

Alton DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Alton, IL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291