DOT Drug Testing - Frankfort, IL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 37 testing centers in the Frankfort area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

37 DOT Drug-Testing Centers in Frankfort

Minutes Away, Test Today

21035 S La Grange Rd

0.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
21035 S La Grange Rd
Frankfort, IL 60423

19070 Everett Blvd, Unit 111

3.36 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
19070 Everett Blvd, Unit 111
Mokena, IL 60448

Po Box 63

3.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Po Box 63
Mokena, IL 60448

18210 La Grange Rd Ste 110

5.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
18210 La Grange Rd Ste 110
Tinley Park, IL 60487

621 E Lincoln Hwy

5.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
621 E Lincoln Hwy
New Lenox, IL 60451

250 E Maple St

5.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
250 E Maple St
New Lenox, IL 60451

6701 159th St Occ Health

6.10 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6701 159th St Occ Health
Tinley Park, IL 60477

17148 Harlem Ave

6.45 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
17148 Harlem Ave
Tinley Park, IL 60477

10737 W. 165th Street

6.75 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 02:00 PM - 04:00 PM
10737 W. 165th Street
Orland Park, IL 60467

25711 S Egyptian Trail

7.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
25711 S Egyptian Trail
Monee, IL 60449

9570 W 159th St Ste A

7.31 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9570 W 159th St Ste A
Orland Park, IL 60467

16325 Harlem Ave Ste 110

7.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
16325 Harlem Ave Ste 110
Tinley Park, IL 60477

19550 Governors Hwy

8.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
19550 Governors Hwy
Flossmoor, IL 60422

19150 South Kedzie Avenue, Suite 101

8.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
19150 South Kedzie Avenue, Suite 101
Flossmoor, IL 60422

Onsite For Poh Only

8.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Onsite For Poh Only
Orland Park, IL 60462

14475 John Humphrey Dr Ste 100,

9.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14475 John Humphrey Dr Ste 100,
Orland Park, IL 60462

14330 S Will Cook Rd

9.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14330 S Will Cook Rd
Homer Glen, IL 60491

3301 N Ashland

10.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3301 N Ashland
Chicago, IL 60657

18665 Dixie Hwy

10.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
18665 Dixie Hwy
Homewood, IL 60430

16501 Kedzie Ave

10.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
16501 Kedzie Ave
Markham, IL 60428

1423 Chicago Rd

10.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1423 Chicago Rd
Chicago Heights, IL 60411

30 E 15th St Ste 100

10.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
30 E 15th St Ste 100
Chicago Heights, IL 60411

16221 W 159th St

11.12 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM • Sat:,Sun: 9:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
16221 W 159th St
Lockport, IL 60441

2025 S Chicago St

12.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2025 S Chicago St
Joliet, IL 60436

611 Division St

12.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
611 Division St
Peotone, IL 60468

12255 S 80th Ave

12.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12255 S 80th Ave
Palos Heights, IL 60463

4742 Cal Sag Rd

12.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4742 Cal Sag Rd
Crestwood, IL 60445

15600 Honore Ave

12.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
15600 Honore Ave
Harvey, IL 60426

1000 S Hamilton St

12.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1000 S Hamilton St
Lockport, IL 60441

7800 W College Dr, Room 1-w

12.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7800 W College Dr, Room 1-w
Palos Heights, IL 60463

4800 W 129th St

12.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4800 W 129th St
Alsip, IL 60803

2310 York St Ste 4a

14.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2310 York St Ste 4a
Blue Island, IL 60406

5533 W 109th St Ste 103

14.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5533 W 109th St Ste 103
Oak Lawn, IL 60453

800 N Larkin Ave

14.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
800 N Larkin Ave
Joliet, IL 60435

2000 Glenwood Ave

14.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2000 Glenwood Ave
Joliet, IL 60435

16101 Weber Rd

14.79 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
16101 Weber Rd
Crest Hill, IL 60403

310 N. Hammes Ave Ste 102,

14.80 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
310 N. Hammes Ave Ste 102,
Joliet, IL 60435

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 37 DOT drug testing locations to serve you in and around Frankfort, IL.

Our DOT Drug Testing locations in Frankfort, IL, and nearby areas offer DOT Drug Testing, Alcohol Testing, and Physicals. These services cater to Employers, Independent operators, and all DOT modes needing to meet 49 CFR Part 40 requirements in Frankfort, IL, including FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Testing centers are conveniently located near you with the potential for same-day service.

Choose your specific DOT service or drug/alcohol test at a nearby center effortlessly for yourself, your team, or others. Our process is streamlined and user-friendly, ensuring that arranging your DOT drug test near Frankfort is straightforward.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Frankfort, Illinois DOT Services

DOT Drug Testing & Services Available in Frankfort, IL:

In Frankfort, IL, our Accredited Drug Testing services provide comprehensive DOT solutions, including drug and alcohol testing, physicals, and consortium enrollment, ensuring compliance and safety for all workforce participants.

DOT Services in Frankfort, IL

In Frankfort, Illinois, DOT regulations ensure that employees in safety-sensitive positions undergo rigorous drug and alcohol screening to maintain the highest safety standards. Employers must adhere to these rules to ensure compliance.

DOT regulations mandate random testing for alcohol and drugs in Frankfort, IL, ensuring impartiality and unpredictability, which strengthens the deterrence of substance abuse among employees.

Frankfort, IL, employers are required by DOT to conduct pre-employment drug tests to screen candidates in safety-sensitive roles, ensuring a drug-free workplace culture from day one.

DOT guidelines in Frankfort, IL, demand post-accident testing to determine if drugs or alcohol played a role in an incident. This is vital for safety evaluations and preventive measures.

Return-to-duty testing overseen by DOT management in Frankfort, IL, ensures employees transitioning back meet strict safety requirements, maintaining a focus on workplace safety.

FMCSA Information

Frankfort, IL follows FMCSA guidelines requiring drug and alcohol tests for commercial vehicle operators, enhancing the safety of interstate transportation.

Employers in Frankfort, IL, must follow FMCSA guidelines involving multi-step testing processes to ensure the safety of road transport operations.

USCG Information

USCG drug and alcohol testing in Frankfort, IL is critical for maritime safety, ensuring crews operate at peak efficiency without impairment.

In Frankfort, IL, USCG regulations enforce drug and alcohol testing to safeguard maritime activities and uphold safety standards across waters.

FAA Information

FAA regulations in Frankfort, IL necessitate regular drug and alcohol screening for aviation employees, ensuring safety in flight operations and passenger security.

Compliance with FAA testing rules in Frankfort, IL is crucial for maintaining the integrity and safety of the aviation industry, with rigorous screening processes in place.

FRA Information

FRA drug and alcohol testing in Frankfort, IL is essential for railway safety, ensuring that all railway personnel adhere to strict substance regulations.

In Frankfort, IL, FRA mandates rigorous testing of railroad employees to prevent substance impairment in critical operational roles.

FTA Information

The FTA guidelines in Frankfort, IL impose drug and alcohol testing to ensure the safety of public transit systems and the well-being of all passengers.

Frankfort, IL adheres to FTA rules to maintain drug-free public transit systems, placing a high priority on passenger and operator safety.

PHMSA Information

Under PHMSA regulations, Frankfort, IL enforces strict drug and alcohol testing for hazardous material handlers, ensuring public and environmental safety.

PHMSA testing in Frankfort, IL mandates strict adherence to drug and alcohol guidelines for all personnel involved with hazardous materials.

Scheduling in Frankfort, IL

For scheduling a DOT drug or alcohol test in Frankfort, IL, call (800) 221-4291. Alternatively, use our online express scheduling system by selecting your test and completing the necessary registration information. Ensure to register before heading to the test center.

Enter your zip code to find the nearest DOT testing location in Frankfort, IL for your specific test. A registration form with location details will be emailed to you. Have it printed or accessible on your mobile for the testing center. Generally, no appointment is needed but you must register and prepay for the test.

Our laboratories hold SAMHSA certification, and test results are verified by our licensed Medical Review Officers (MRO) in-house.

Besides offering DOT drug and alcohol testing in Frankfort, IL, Accredited Drug Testing provides services throughout the metropolitan region and surrounding areas.

Whether traveling or on a break, rely on Accredited Drug Testing with centers nationwide to schedule your test anytime, anywhere!

Illinois Dept. of Transportation Updates

Frankfort, IL DUI Statistics

Frankfort, located in Illinois (IL), is subject to data collection by the Department of Transportation (DOT) regarding DUI incidents. In Will County, where Frankfort is situated, DUI statistics have been a point of focus. Law enforcement agencies in Illinois consistently monitor DUIs to mitigate traffic-related injuries and fatalities. The results from recent years demonstrate a fluctuation in DUI occurrences, with education and strict enforcement playing roles in the downward trend. Nevertheless, alcohol-impaired driving continues to pose significant challenges. Illinois (IL) remains vigilant in its efforts to reduce these incidents through public awareness campaigns, sobriety checkpoints, and enhanced legal measures aimed at deterring impaired driving.

Frankfort, IL Drug-Involved Accidents

Frankfort, IL, notably within the jurisdiction of Will County, has seen varied trends in drug-involved vehicular accidents. The Illinois (IL) Department of Transportation (DOT) identifies drug-related accidents as a key area of concern. These incidents encompass a range of substances, including prescription drugs, which impair driving abilities. Over the past few years, law enforcement and public safety initiatives have been intensified in Frankfort to address this issue. IL's DOT collaborates with local police departments to educate the public and implement interventions. Despite these measures, drug-involved accidents remain a significant risk factor on Frankfort roads, prompting ongoing efforts to curb their occurrence.

Frankfort, IL Marijuana-Related Accidents

In recent years, Frankfort, IL, and surrounding areas within Will County, have experienced increasing attention on marijuana-related traffic accidents. Since Illinois legalized recreational marijuana, the state has faced new challenges in managing its impact on road safety. The IL Department of Transportation (DOT) tracks such incidents to assess changes in traffic safety dynamics. Frankfort's local law enforcement works closely with statewide initiatives to address driving under the influence of marijuana, employing education campaigns and advanced training for officers to recognize marijuana impairment. While progress is being made, marijuana-related accidents represent an evolving threat within the wider context of vehicular safety in Illinois (IL).

Frankfort Drug Testing

Quickly find trusted local drug testing centers in Frankfort, IL — fast, convenient, and reliable every time!

Frankfort DOT Drug Testing

Quickly find a local DOT drug testing center in Frankfort, IL — fast, reliable, convenient nationwide service!

Frankfort DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Frankfort, IL.

Frankfort Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Frankfort, IL.v

Frankfort Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Frankfort, IL locations—results you can trust, every time!

Frankfort Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Frankfort, IL.

Frankfort Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Frankfort, IL.

Frankfort 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Frankfort, IL employers and individuals nationwide.

Frankfort 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Frankfort, IL workplace and personal testing needs.

Frankfort On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Frankfort, IL workplace compliant.

Frankfort DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Frankfort, IL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291