DOT Drug Testing - Jacksonville, IL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 25 testing centers in the Jacksonville area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

25 DOT Drug-Testing Centers in Jacksonville

Minutes Away, Test Today

219 Dunlap Ct

0.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
219 Dunlap Ct
Jacksonville, IL 62650

901 W Morton Ave 16a

0.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
901 W Morton Ave 16a
Jacksonville, IL 62650

1300 Lincoln Ave

1.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1300 Lincoln Ave
Jacksonville, IL 62650

273 N Westgate Ave,

1.79 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
273 N Westgate Ave,
Jacksonville, IL 62650

100 W 15th St

21.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
100 W 15th St
Beardstown, IL 62618

507 Washington St

22.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
507 Washington St
Beardstown, IL 62618

3119 Robbins Rd,

27.54 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-12:00 PM & 1:00 PM-3:00 PM✓ Walk-in with Electronic Donor Pass
3119 Robbins Rd,
Springfield, IL 62704

993 Clock Tower Dr Ste C

29.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
993 Clock Tower Dr Ste C
Springfield, IL 62704

1305 Wabash Ave Ste F

29.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1305 Wabash Ave Ste F
Springfield, IL 62704

1836 S Macarthur Blvd

29.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1836 S Macarthur Blvd
Springfield, IL 62704

775 Engineering Dr

31.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
775 Engineering Dr
Springfield, IL 62703

800 School St

31.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
800 School St
Carrollton, IL 62016

238 S Congress St

31.96 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
238 S Congress St
Rushville, IL 62681

640 W Washington St

32.55 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
640 W Washington St
Pittsfield, IL 62363

521 E Main St

33.79 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
521 E Main St
Mt Sterling, IL 62353

20733 N Broad St

35.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
20733 N Broad St
Carlinville, IL 62626

X X

36.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
X X
Carlinville, IL 62626

615 N Promenade St

40.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
615 N Promenade St
Havana, IL 62644

400 Maple Summit Rd

43.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
400 Maple Summit Rd
Jerseyville, IL 62052

2967 N 2600th Ave

46.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2967 N 2600th Ave
La Prairie, IL 62346

211 S 3rd St

47.79 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
211 S 3rd St
Louisiana, MO 63353

608 W Kirkham St

48.96 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
608 W Kirkham St
Litchfield, IL 62056

312 N State St

49.20 mi
🕑 Testing Hours: M,F 8:00 AM-12:00 PM & 1:00 PM-5:30 PM • T,Sat: 8:00 AM-11:30 AM • W 8:00 AM-12:00 PM & 3:00 PM-6:30 PM✓ Walk-in with Electronic Donor Pass
312 N State St
Litchfield, IL 62056

1285 Franciscan Dr

49.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1285 Franciscan Dr
Litchfield, IL 62056

1215 Franciscan Dr

49.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1215 Franciscan Dr
Litchfield, IL 62056

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 25 DOT drug testing locations to serve you in and around Jacksonville, IL.

Our DOT Drug Testing sites in Jacksonville, IL and nearby cities offer comprehensive DOT services—such as Drug Testing, Alcohol Testing, Physicals, Consortium enrollment, MIS reports, MVR, and driver qualification reports—for employers, owner-operators, and other entities that must follow 49 CFR Part 40 rules, covering FMCSA, FTA, FAA, USCG, FRA, and PHMSA in Jacksonville, IL. Services can often be arranged for the same day.

Choose your required DOT service or drug/alcohol test and select a convenient location. Whether for yourself, an employee, or someone else, our straightforward process simplifies arranging DOT drug testing near Jacksonville.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Jacksonville, Illinois DOT Services

DOT Drug Testing & Services Available in Jacksonville, IL:

At Accredited Drug Testing in Jacksonville, IL, our DOT services cover comprehensive drug and alcohol testing, ensuring compliance with federal regulations. We facilitate seamless scheduling, reinforcing workplace safety and compliance.

DOT Services in Jacksonville, IL

DOT regulations stress safety with stringent drug and alcohol screening requirements. In Jacksonville, IL, these rules help maintain safe operations for commercial drivers through regular testing.

Employees in safety-sensitive roles in Jacksonville, Illinois must comply with DOT drug and alcohol testing, ensuring a secure transport environment for all.

Stringent DOT testing regulations in Jacksonville, IL focus on preventing substance misuse among transportation employees, vital for public safety and compliance.

Jacksonville, Illinois adheres to DOT guidelines requiring consistent drug and alcohol testing, safeguarding employees and the public with a focus on secure transport operations.

Regular screenings under DOT rules in Jacksonville, IL are pivotal in reinforcing a culture of safety and reliability for transport-sector employees and employers alike.

FMCSA Information

In Jacksonville, IL, FMCSA mandates drug and alcohol testing for all commercial drivers to ensure safety and regulatory adherence across the roadways.

FMCSA guidelines in Jacksonville, Illinois stipulate regular testing, crucial for maintaining compliance and safe driving practices for commercial operators.

USCG Information

USCG mandates drug and alcohol testing for maritime employees in Jacksonville, IL to uphold safety and prevention of substance misuse on the waterways.

Jacksonville, Illinois complies with USCG rules, ensuring maritime safety through scheduled and random drug and alcohol testing protocols.

FAA Information

FAA regulations in Jacksonville, IL mandate drug and alcohol testing for aviation employees, ensuring safety and adherence to federal compliance standards.

Air transportation safety in Jacksonville, Illinois relies on FAA-mandated testing to prevent substance misuse among aviation personnel.

FRA Information

FRA regulations in Jacksonville, IL enforce strict drug and alcohol testing for rail employees, crucial for ensuring rail safety and operational compliance.

Railroad safety in Jacksonville, Illinois is upheld by FRA testing rules, prioritizing safe practices through rigorous screening procedures.

FTA Information

FTA rules in Jacksonville, IL mandate drug and alcohol testing for transit employees to maintain public safety and comply with federal transport standards.

Jacksonville, Illinois transit systems implement FTA testing to ensure reliable and secure public transport through effective drug and alcohol testing enforcement.

PHMSA Information

Jacksonville, IL follows PHMSA rules requiring drug and alcohol testing for pipeline workers, focusing on safety and preventing substance misuse.

PHMSA regulations in Jacksonville, Illinois ensure safe pipeline operations through mandatory employee testing and strict compliance checks.

Scheduling in Jacksonville, IL

To arrange a DOT drug or alcohol test in Jacksonville, IL, contact our local scheduling at (800) 221-4291 or use our online express scheduling by selecting the test and filling out the Donor Information/Registration Section. Registration is required before visiting a testing site.

The zip code you enter identifies the closest testing center near Jacksonville, IL that offers the tests you need. A registration form with local center details, including address and hours, will be emailed to you. Carry this form or display it on your phone at the center. Appointments are mostly unnecessary, but donor registration and test payment are needed beforehand.

Our laboratories hold SAMHSA certification; all results undergo verification by licensed in-house Medical Review Officers (MRO).

Besides providing DOT testing in Jacksonville, IL, Accredited Drug Testing operates other testing sites across the metro and neighboring areas.

Wherever you are in the U.S., Accredited Drug Testing has centers to meet your testing needs—any location, any time!

Illinois Dept. of Transportation Updates

DUI Statistics in Jacksonville, IL

Jacksonville, Illinois, located within Morgan County, is not immune to the issues of driving under the influence (DUI). Illinois state law is stringent when it comes to DUI enforcement, aiming to reduce incidents and enhance road safety. In recent years, the Jacksonville Police Department has stepped up its efforts, conducting extensive DUI checkpoints and awareness campaigns. The state of Illinois, with its zero-tolerance policy for under-21 drivers, also plays a crucial role. In Morgan County, DUI arrests have shown a varied pattern, reflecting broader Illinois statewide trends. Collaboration with organizations such as Mothers Against Drunk Driving (MADD) in Illinois further strengthens community efforts in Jacksonville to tackle DUI challenges head-on.

Drug-Involved Accidents in Jacksonville, IL

In Jacksonville, Illinois, drug-involved accidents represent a significant concern for road safety advocates and law enforcement officials alike. Morgan County has seen various initiatives aimed at tackling drug-related road incidents. The Illinois Department of Transportation (IDOT) collaborates with local Jacksonville authorities to support these measures, which include educational programs about the dangers of drug-impaired driving. Awareness campaigns highlight the risks associated with driving under the influence of drugs, emphasizing penalties under Illinois laws. Recent statistics in the Jacksonville region suggest a need for continued vigilance, as state and county authorities work to reduce the frequency and impact of drug-involved road accidents. The role of community agencies in Jacksonville in educating the public is pivotal in these efforts.

Marijuana-Related Accidents in Jacksonville, IL

Since the legalization of recreational marijuana in Illinois, Jacksonville, situated in Morgan County, has, like many other areas, been monitoring its impact on driving safety. The Jacksonville Police Department and state-level agencies such as the Illinois State Police are diligently analyzing marijuana-related traffic incidents. The Illinois state government supports research on impairment thresholds and driver education to mitigate risks. In Jacksonville and the broader Illinois community, there's a focus on public awareness about the responsibilities of marijuana use and driving, supplemented by legal penalties for offenders. This dual strategy aims to prevent a rise in marijuana-related accidents, safeguarding both residents and visitors on Illinois roads.

Jacksonville Drug Testing

Quickly find trusted local drug testing centers in Jacksonville, IL — fast, convenient, and reliable every time!

Jacksonville DOT Drug Testing

Quickly find a local DOT drug testing center in Jacksonville, IL — fast, reliable, convenient nationwide service!

Jacksonville DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Jacksonville, IL.

Jacksonville Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Jacksonville, IL.v

Jacksonville Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Jacksonville, IL locations—results you can trust, every time!

Jacksonville Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Jacksonville, IL.

Jacksonville Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Jacksonville, IL.

Jacksonville 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Jacksonville, IL employers and individuals nationwide.

Jacksonville 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Jacksonville, IL workplace and personal testing needs.

Jacksonville On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Jacksonville, IL workplace compliant.

Jacksonville DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Jacksonville, IL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291