DOT Drug Testing - Dayton, OH

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 32 testing centers in the Dayton area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

32 DOT Drug-Testing Centers in Dayton

Minutes Away, Test Today

228 Troy St

0.31 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
228 Troy St
Dayton, OH 45404

1 Elizabeth Pl Ste 250

0.88 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1 Elizabeth Pl Ste 250
Dayton, OH 45408

1435 Cincinnati St Ste 100

1.84 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:30 PM✓ Walk-in with Electronic Donor Pass
1435 Cincinnati St Ste 100
Dayton, OH 45417

425 N Findlay St Ste 315

1.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
425 N Findlay St Ste 315
Dayton, OH 45404

1221 Wilmington Ave Ste 211

2.46 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1221 Wilmington Ave Ste 211
Dayton, OH 45420

2555 S Dixie Dr Ste 101b

2.53 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
2555 S Dixie Dr Ste 101b
Dayton, OH 45409

2308 Sandridge Dr

2.97 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM • Sat: 8:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
2308 Sandridge Dr
Moraine, OH 45439

247 Forrer Blvd

3.18 mi
🕑 Testing Hours: M-Sun: 8:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
247 Forrer Blvd
Dayton, OH 45419

2600 Far Hills Ave Ste 100

3.74 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM • Sat:,Sun: 9:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
2600 Far Hills Ave Ste 100
Dayton, OH 45419

1010 Woodman Dr

3.84 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
1010 Woodman Dr
Dayton, OH 45432

2023 Springboro W

3.91 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
2023 Springboro W
Moraine, OH 45439

3019 Production Ct

4.46 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-7:00 PM • Sat: 10:00 AM-1:30 PM • Sun: 10:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
3019 Production Ct
Dayton, OH 45414

540 Lincoln Park Blvd Ste 170

4.57 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
540 Lincoln Park Blvd Ste 170
Kettering, OH 45429

5915 N Main St

4.93 mi
🕑 Testing Hours: M-Sun: 8:00 AM-7:00 PM✓ Walk-in with Electronic Donor Pass
5915 N Main St
Dayton, OH 45415

3604 Wilmington Pike

5.23 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
3604 Wilmington Pike
Kettering, OH 45429

1525 E. Stroop Road Ste 101

5.62 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1525 E. Stroop Road Ste 101
Kettering, OH 45429

3464 Pentagon Park Blvd, Ste E

5.65 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3464 Pentagon Park Blvd, Ste E
Beavercreek, OH 45431

6210 Brandt Pike

7.03 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
6210 Brandt Pike
Huber Heights, OH 45424

2601 Commons Blvd

7.04 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
2601 Commons Blvd
Beavercreek, OH 45431

3371 Kemp Rd

7.15 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-12:00 PM & 1:00 PM-3:30 PM✓ Walk-in with Electronic Donor Pass
3371 Kemp Rd
Dayton, OH 45431

1244 Meadow Bridge Dr

7.77 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-2:30 PM✓ Walk-in with Electronic Donor Pass
1244 Meadow Bridge Dr
Beavercreek, OH 45434

7381 Brandt Pike

8.25 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:30 PM (Lunch: 01:00 PM - 01:30 PM)⚡ Drug Screens: Mon–Fri: 08:00 AM - 01:00 PM, 02:00 PM - 04:30 PM
7381 Brandt Pike
Dayton, OH 45424

6625 Centerville Business Park

8.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6625 Centerville Business Park
Centerville, OH 45459

2350 Miami Valley Dr

8.47 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-3:30 PM✓ Walk-in with Electronic Donor Pass
2350 Miami Valley Dr
Centerville, OH 45459

5 W Wenger Rd

8.71 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 07:30 AM - 03:00 PM
5 W Wenger Rd
Englewood, OH 45322

7901 Schatz Pointe Dr

8.77 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
7901 Schatz Pointe Dr
Centerville, OH 45459

8701 Troy Pike

8.81 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
8701 Troy Pike
Huber Heights, OH 45424

415 Byers Rd Ste 200

8.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
415 Byers Rd Ste 200
Miamisburg, OH 45342

8210 Springboro Pike

9.07 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 12:00 PM, 01:30 PM - 04:00 PM
8210 Springboro Pike
Miamisburg, OH 45342

10871 Engle Rd

10.16 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM✓ Walk-in with Electronic Donor Pass
10871 Engle Rd
Vandalia, OH 45377

2131 Gateway Dr

10.21 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM (Lunch: 12:00 PM - 12:30 PM)⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 01:30 PM - 03:00 PM
2131 Gateway Dr
Fairborn, OH 45324

1836 Commerce Center Blvd

10.46 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-12:00 PM & 1:30 PM-3:30 PM✓ Walk-in with Electronic Donor Pass
1836 Commerce Center Blvd
Fairborn, OH 45324

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 32 DOT drug testing locations to serve you in and around Dayton, OH.

Our Dayton, OH drug screening facilities offer DOT Drug Testing, DOT Alcohol Testing, DOT Physicals, random pool and consortium enrollment, MIS and MVR reports, and other required DOT services for employers and individual operators. We ensure compliance with 49 CFR Part 40 for FMCSA, FTA, FAA, USCG, FRA, and PHMSA across all DOT modes in Dayton, OH. Locating a testing center is convenient, with same-day operational services available.

Choose the desired DOT service or test, and select the nearest location for either personal, employee, or third-party testing. Our efficient system simplifies the process of arranging DOT drug testing near Dayton.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Dayton, Ohio DOT Services

DOT Drug Testing & Services Available in Dayton, OH:

In Dayton, OH, Accredited Drug Testing offers comprehensive DOT services including drug and alcohol screenings. Our dedicated centers ensure efficient, compliant testing for local individuals and businesses.

DOT Services in Dayton, OH

The DOT mandates stringent drug and alcohol screenings to promote employee safety and compliance in Dayton, OH and beyond. Employers must ensure their workforce meets all safety regulations.

Regular random testing helps identify substance misuse, safeguarding not only individual health but also public safety in Dayton, Ohio workplaces.

Complying with DOT regulations in Dayton, OH, organizations can prevent incidents arising from drug or alcohol impairment, maintaining operational integrity.

It's vital for businesses in Dayton, Ohio, to integrate these screenings as part of their safety protocols, assuring adherence to DOT-established guidelines.

FMCSA Information

FMCSA requires stringent drug testing compliance to ensure safety on Dayton, OH roads. All CDL drivers must undergo regular screenings to meet federal safety standards.

In Dayton, Ohio, our FMCSA testing ensures drivers remain unimpaired, promoting safe transport within the national highway system.

USCG Information

USCG protocols require routine testing of marine personnel in Dayton, OH to ensure safety and operational efficacy of maritime operations.

By adhering to USCG standards, Dayton, Ohio waters remain safe, reducing the risk of accidents from crew impairment.

FAA Information

To maintain aviation safety in Dayton, OH, FAA demands regular drug and alcohol screenings for all aviation staff, ensuring passenger and staff security at all times.

Accredited Drug Testing supports Dayton, Ohio aviation sectors, keeping professionals compliant and skies safe through effective testing protocols.

FRA Information

By FRA standards, rail workers in Dayton, OH must submit to rigorous testing to guarantee rail safety and operational integrity along key routes.

Ensuring safety on the tracks in Dayton, Ohio involves adherence to FRA's strict drug and alcohol policy enforcement.

FTA Information

FTA guidelines aim to maintain public transit safety in Dayton, OH through comprehensive random drug testing for all transit employees.

Ensuring a safe public transport system in Dayton, Ohio relies on consistent adherence to FTA regulations by all transit agencies.

PHMSA Information

PHMSA regulations mandate rigorous testing protocols for pipeline workers in Dayton, OH. This aids in preventing accidents and maintaining safety standards.

Ensuring compliance in Dayton, Ohio is crucial for safety in the transportation of hazardous materials, reducing potential risks.

Scheduling in Dayton, OH

To arrange a DOT drug or alcohol test in Dayton, OH, contact us at (800) 221-4291, or use our express system online by selecting your test and completing the registration section. Registration is required before visiting the center.

The zip code provided will identify the nearest testing center performing your chosen test in Dayton, Ohio. You will receive a donor pass via email with details and center address—no prior appointment needed, but registration and payment are necessary.

With SAMHSA-certified labs, our Medical Review Officers, licensed physicians, verify every test result securely and professionally.

Accredited Drug Testing's extensive network in and around Dayton, OH ensures easy access to DOT drug and alcohol testing services across all regions.

No matter where you are, our reach extends nationwide, offering testing locations in countless cities, facilitating scheduling anytime, anywhere!

Ohio Dept. of Transportation Updates

Dayton, OH DUI Statistics

In Dayton, OH, the issue of driving under the influence (DUI) continues to be a concern for the Department of Transportation (DOT) and local law enforcement agencies. Montgomery County, where Dayton is located, has implemented several initiatives to curb this problem, including public awareness campaigns and increased patrols during peak times. According to recent data, while there has been a slight decline in DUI incidents over the past few years, the number remains significant, with alcohol-impaired driving deaths accounting for approximately 30% of all traffic fatalities in Ohio (OH). Efforts are ongoing to further decrease these numbers through stricter enforcement and community engagement programs.

Drug-Involved Accidents in Dayton, OH

Drug involvement in traffic accidents is a growing concern in Dayton, OH, and the surrounding Montgomery County. The Ohio Department of Transportation (ODOT) has reported an increase in accidents where drivers tested positive for substances other than alcohol. This trend is alarming and has prompted local authorities to enhance drug recognition training for officers and collaborate with healthcare professionals to address substance abuse. Dayton has seen a rise in polydrug use, where individuals consume multiple substances, complicating enforcement and prevention efforts. Statewide, Ohio recognizes this issue, implementing policies aimed at reducing drug-impaired driving through education and stricter penalties.

Marijuana-Related Accidents in Dayton, OH

With the increasing legalization and decriminalization of marijuana across various states, including Ohio, there has been a notable impact on road safety in Dayton, OH. The Ohio Department of Transportation (ODOT) and local Dayton authorities have observed a rise in marijuana-involved traffic incidents. Although marijuana does not account for the majority of impaired driving cases, its role is significant enough to warrant concern. Law enforcement agencies in Montgomery County are conducting studies to better understand the relationship between marijuana use and accident rates. These efforts are part of a broader strategy that includes impairment assessments and public education campaigns on the risks associated with driving under the influence of marijuana.

Dayton Drug Testing

Quickly find trusted local drug testing centers in Dayton, OH — fast, convenient, and reliable every time!

Dayton DOT Drug Testing

Quickly find a local DOT drug testing center in Dayton, OH — fast, reliable, convenient nationwide service!

Dayton DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Dayton, OH.

Dayton Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Dayton, OH.v

Dayton Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Dayton, OH locations—results you can trust, every time!

Dayton Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Dayton, OH.

Dayton Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Dayton, OH.

Dayton 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Dayton, OH employers and individuals nationwide.

Dayton 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Dayton, OH workplace and personal testing needs.

Dayton On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Dayton, OH workplace compliant.

Dayton DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Dayton, OH drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291