DOT Drug Testing - Lancaster, PA

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 36 testing centers in the Lancaster area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

36 DOT Drug-Testing Centers in Lancaster

Minutes Away, Test Today

555 N Duke St

0.64 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
555 N Duke St
Lancaster, PA 17602

1100 E Orange St

1.34 mi
🕑 Testing Hours: Mon–Sat: 8:00 AM-8:00 PM • Sun: 10:00 AM-6:00 PM✓ Walk-in with Electronic Donor Pass
1100 E Orange St
Lancaster, PA 17602

241 Rohrerstown Rd 2nd Fl Ste 200

1.40 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:30 PM • Sat: 8:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
241 Rohrerstown Rd 2nd Fl Ste 200
Lancaster, PA 17603

113 Butler Ave

2.00 mi
🕑 Testing Hours: M,T,Th,F 8:00 AM-5:00 PM • W 7:30 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
113 Butler Ave
Lancaster, PA 17601

150 Farmington Ln Ste 201

2.51 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-5:30 PM✓ Walk-in with Electronic Donor Pass
150 Farmington Ln Ste 201
Lancaster, PA 17601

2148 Embassy Dr

2.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2148 Embassy Dr
Lancaster, PA 17603

215 Granite Run Dr,

3.07 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-3:20 PM • Sat: 6:00 AM-11:40 AM✓ Walk-in with Electronic Donor Pass
215 Granite Run Dr,
Lancaster, PA 17601

4 Rohrerstown Rd

3.16 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
4 Rohrerstown Rd
Lancaster, PA 17603

2110 Harrisburg Pike Ste 21

3.47 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-7:00 PM • Sat: 12:00 PM-7:00 PM✓ Walk-in with Electronic Donor Pass
2110 Harrisburg Pike Ste 21
Lancaster, PA 17601

235 Bloomfield Dr

5.16 mi
🕑 Testing Hours: Mon–Fri: 12:00 PM-2:00 PM✓ Walk-in with Electronic Donor Pass
235 Bloomfield Dr
Lititz, PA 17543

1500 Highlands Dr

6.75 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-6:00 PM✓ Walk-in with Electronic Donor Pass
1500 Highlands Dr
Lititz, PA 17543

4237 Oregon Pike

7.39 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
4237 Oregon Pike
Ephrata, PA 17522

112 N Reading Rd,

12.14 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-2:00 PM • Sat: 7:00 AM-10:30 AM✓ Walk-in with Electronic Donor Pass
112 N Reading Rd,
Ephrata, PA 17522

446 N Reading Rd

12.91 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
446 N Reading Rd
Ephrata, PA 17522

435 S Kinzer Ave

12.92 mi
🕑 Testing Hours: M-Th 8:30 AM-4:30 PM • F 8:30 AM-12:30 PM✓ Walk-in with Electronic Donor Pass
435 S Kinzer Ave
New Holland, PA 17557

836 Houston Run Dr Ste 201

14.59 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
836 Houston Run Dr Ste 201
Gap, PA 17527

5275 Lincoln Hwy

15.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5275 Lincoln Hwy
Gap, PA 17527

418 Cloverleaf Rd

15.13 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
418 Cloverleaf Rd
Elizabethtown, PA 17022

645 N Mount Joy St

18.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
645 N Mount Joy St
Elizabethtown, PA 17022

199 S 4th St At 399 Walnut St

18.83 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
199 S 4th St At 399 Walnut St
Lebanon, PA 17042

1 E Beacon Light Ln

20.25 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
1 E Beacon Light Ln
Chester, PA 19013

950 Octorara Trail

20.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
950 Octorara Trail
Parkesburg, PA 19365

1010 W Crestview Dr

20.38 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1010 W Crestview Dr
Lebanon, PA 17042

2250 E Market St

20.52 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-6:00 PM • Sat:,Sun: 9:00 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
2250 E Market St
York, PA 17402

1748 6th Ave,

21.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1748 6th Ave,
York, PA 17403

325 S Belmont St

21.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
325 S Belmont St
York, PA 17403

239 Pauline Dr (s York Plaza)

21.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
239 Pauline Dr (s York Plaza)
York, PA 17402

615 Cumberland St

21.74 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:30 PM✓ Walk-in with Electronic Donor Pass
615 Cumberland St
Lebanon, PA 17042

Rt 422 West,

22.04 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-11:30 AM & 12:00 PM-2:00 PM✓ Walk-in with Electronic Donor Pass
Rt 422 West,
Lebanon, PA 17042

102 Friends Meeting House Rd

22.62 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-6:00 PM✓ Walk-in with Electronic Donor Pass
102 Friends Meeting House Rd
Cochranville, PA 19330

11 E Market St Ste 104

22.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11 E Market St Ste 104
York, PA 17401

400 Loucks Rd

23.51 mi
🕑 Testing Hours: M-Sun: 8:00 AM-6:00 PM✓ Walk-in with Electronic Donor Pass
400 Loucks Rd
York, PA 17404

2168 Cloverfield Dr

23.88 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-11:00 AM & 1:00 PM-3:00 PM✓ Walk-in with Electronic Donor Pass
2168 Cloverfield Dr
Lebanon, PA 17046

725 E Lincoln Ave,

24.01 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-1:30 PM • Sat: 7:00 AM-10:30 AM✓ Walk-in with Electronic Donor Pass
725 E Lincoln Ave,
Myerstown, PA 17067

970 Loucks Rd Unit D

24.06 mi
🕑 Testing Hours: M-Th 9:00 AM-5:00 PM • F 9:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
970 Loucks Rd Unit D
York, PA 17404

520 Greenbriar Rd

24.40 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-6:00 PM✓ Walk-in with Electronic Donor Pass
520 Greenbriar Rd
York, PA 17404

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 36 DOT drug testing locations to serve you in and around Lancaster, PA.

Our Lancaster, PA DOT Testing locations and nearby areas offer a wide array of DOT services including Drug and Alcohol Testing, Physicals, and more. We serve employers, single operators, and all DOT modalities in compliance with 49 CFR Part 40 requirements across FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Conveniently situated testing centers ensure swift access, often providing same-day service.

Choose your desired service or test and pick a location close to you—whether for personal, employee, or another's needs. Our efficient and straightforward booking system lets you arrange DOT drug testing around Lancaster with ease.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Lancaster, Pennsylvania DOT Services

DOT Drug Testing & Services Available in Lancaster, PA:

Accredited Drug Testing offers comprehensive DOT testing services in Lancaster, PA, covering all required drug and alcohol tests. We facilitate easy access to services for employers and employees, ensuring compliance with federal regulations and promoting workplace safety in Lancaster, Pennsylvania.

DOT Services in Lancaster, PA

DOT regulations ensure a drug-free and safe working environment through strict screening policies. Employers in Lancaster, PA must adhere to federal guidelines by frequently conducting random drug and alcohol tests on safety-sensitive employees to prevent accidents and ensure public safety.

Regulations require comprehensive education for employees and supervisors about the effects of drug and alcohol misuse. Lancaster, PA institutions are mandated to offer training programs that fulfill DOT criteria, enhancing workplace awareness and safety protocols.

In Lancaster, Pennsylvania, DOT regulations mandate the development of a written policy regarding drug and alcohol testing that is communicated to all employees. This policy must detail the testing process, employee rights, and consequences of positive results, ensuring transparency and adherence.

Employers in Lancaster, PA must maintain a secure infrastructure for testing and reporting processes according to DOT standards. Accurate records of testing results and related documentation are to be meticulously kept to verify compliance with federal regulations and uphold industry safety standards.

FMCSA Information

In Lancaster, PA, DOT FMCSA guidelines demand regular drug testing for commercial drivers to enhance road safety. Compliance ensures a safer travel environment throughout Pennsylvania's roads.

Employers must maintain an FMCSA-compliant drug testing program in Lancaster, Pennsylvania, ensuring thorough monitoring of commercial drivers for substance abuse for community safety.

USCG Information

USCG regulations require regular drug and alcohol testing for maritime personnel in Lancaster, PA. Ensuring clean records for seafarers protects both employees and waters they navigate.

In Lancaster, Pennsylvania, USCG standards dictate stringent testing for maritime workers to maintain secure, efficient maritime operations and uphold maritime safety statutes.

FAA Information

FAA regulations in Lancaster, PA insist on rigorous drug and alcohol testing for aviation personnel, fostering a reliable and secure flying environment. Compliance is critical to air travel safety.

Within Lancaster, Pennsylvania, FAA standards mandate regular screening of pilots and aviation staff, ensuring controlled, substance-free operations and enhancing overall flight safety.

FRA Information

FRA's regulations in Lancaster, PA demand regular drug and alcohol testing for rail workers, safeguarding train operations. Compliance guarantees the safety of rail networks across the state.

In Lancaster, Pennsylvania, the FRA enforces strict testing protocols on railway personnel to ensure safe operation, maintaining the integrity of the state's rail transport system.

FTA Information

FTA mandates drug and alcohol screening in Lancaster, PA's public transit workforce to protect passengers. Adhering to FTA rules sustains transit system safety and reliability throughout the area.

In Lancaster, Pennsylvania, FTA regulations ensure each transit employee undergoes testing, thereby ensuring smooth, reliable, and safe public transportation operations.

PHMSA Information

In Lancaster, PA, PHMSA upholds stringent drug and alcohol testing rules to safeguard the pipeline transport sector. Employers must ensure compliance to protect public health and infrastructure.

PHMSA rules in Lancaster, Pennsylvania, require routine screening of pipeline workers, preventing substance misuse and ensuring consistent safety in energy transport.

Scheduling in Lancaster, PA

To arrange a DOT drug or alcohol test in Lancaster, PA, contact our local team at (800) 221-4291 or use our online scheduling tool to register for the test. Pre-registration is mandatory before visiting any testing center.

When you enter your zip code, we'll identify the nearest testing site in Lancaster, Pennsylvania, offering your required test. We will email a donor pass/registration form, including directions and operational details of the testing center. Present the form, either printed or on your smartphone, at your visit. Usually, no appointments are necessary, though registration and payment are prerequisites.

Our testing laboratories boast SAMHSA certification, ensuring all results are authenticated by our in-house Medical Review Officers (MRO), who are licensed physicians.

Besides our Lancaster locations, Accredited Drug Testing operates additional centers throughout the greater metropolitan area and nearby cities.

Whether you're traveling or on a break, Accredited Drug Testing has you covered nationwide with numerous testing centers ready to assist wherever and whenever you need testing.

Pennsylvania Dept. of Transportation Updates

DUI Statistics in Lancaster, PA

The city of Lancaster, situated in Lancaster County, Pennsylvania, has unfortunately seen a notable number of DUI-related incidents over the years. Due to its location as a central hub in the state, Lancaster, PA experiences a higher volume of vehicular traffic which in turn has affected DUI statistics. Law enforcement agencies in Pennsylvania report that Lancaster County has experienced a steady increase in DUI arrests, partly attributed to the growing population and burgeoning nightlife. Initiatives have been rolled out to educate residents and reduce these figures but challenges remain. The Pennsylvania Department of Transportation (PennDOT) continues to work closely with local agencies to address and mitigate this ongoing issue by deploying targeted campaigns aimed at heightening awareness and encouraging safe driving practices.

Drug-Involved Accidents in Lancaster County, PA

Lancaster County, Pennsylvania, has been grappling with the ramifications of drug-involved traffic accidents, a concern that parallels a nationwide trend. In Lancaster, PA, and the broader county region, there has been an uptick in accidents where drug impairment was a contributing factor. This troubling trend has been attributed largely to the accessibility of illicit substances and the public's growing dependence on prescription drugs. In response, law enforcement agencies in Pennsylvania have intensified efforts to identify drugged driving through enhanced training and better-equipped roadside tests. Lancaster, PA's authorities, working in collaboration with PennDOT, are pushing forward with community outreach, urging citizens to remain vigilant and report any suspicious driving behavior. Despite these efforts, Pennsylvania continues to monitor and adapt strategies to effectively curtail drug-related vehicular incidents.

Marijuana-Related Accidents in Lancaster County, PA

The impact of marijuana legalization in several states, including Pennsylvania, has led to heightened scrutiny of its effects on traffic safety in Lancaster County. In Lancaster, PA, while there is no direct legalization of recreational marijuana, the decriminalization measures and medicinal cannabis usage have had a notable impact. Law enforcement officials in Pennsylvania have reported concerns about an increase in marijuana-related accidents. Lancaster, PA's police departments, along with PennDOT, have been proactive, implementing specialized training programs to help officers recognize signs of marijuana impairment. Despite the regulatory framework set to manage marijuana usage in Pennsylvania, dialogues are ongoing to better educate the public about the risks associated with driving under the influence of cannabis. This multifaceted approach aims to minimize accident statistics linked to marijuana impairment in Lancaster County, PA.

Lancaster Drug Testing

Quickly find trusted local drug testing centers in Lancaster, PA — fast, convenient, and reliable every time!

Lancaster DOT Drug Testing

Quickly find a local DOT drug testing center in Lancaster, PA — fast, reliable, convenient nationwide service!

Lancaster DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Lancaster, PA.

Lancaster Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Lancaster, PA.v

Lancaster Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Lancaster, PA locations—results you can trust, every time!

Lancaster Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Lancaster, PA.

Lancaster Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Lancaster, PA.

Lancaster 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Lancaster, PA employers and individuals nationwide.

Lancaster 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Lancaster, PA workplace and personal testing needs.

Lancaster On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Lancaster, PA workplace compliant.

Lancaster DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Lancaster, PA drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291