DOT Drug Testing - Pittsburgh, PA

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 40 testing centers in the Pittsburgh area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

40 DOT Drug-Testing Centers in Pittsburgh

Minutes Away, Test Today

339 6th Ave 5th Fl

0.17 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
339 6th Ave 5th Fl
Pittsburgh, PA 15222

526 Penn Avenue,

0.38 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
526 Penn Avenue,
Pittsburgh, PA 15222

1515 Locust St

0.62 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-11:30 AM & 12:00 PM-4:00 PM✓ Walk-in with Electronic Donor Pass
1515 Locust St
Pittsburgh, PA 15219

490 E North Ave Ste 106

1.14 mi
🕑 Testing Hours: Mon–Fri: 10:00 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
490 E North Ave Ste 106
Pittsburgh, PA 15212

320 E North Ave

1.15 mi
🕑 Testing Hours: Mon–Fri: 10:00 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
320 E North Ave
Pittsburgh, PA 15212

1600 W Carson St

1.85 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1600 W Carson St
Pittsburgh, PA 15219

3500 5th Avenue, Suite G1

1.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3500 5th Avenue, Suite G1
Pittsburgh, PA 15213

120 Lytton Avenue, Suite 100 C

2.19 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
120 Lytton Avenue, Suite 100 C
Pittsburgh, PA 15213

120 Lytton Ave Ste 275

2.19 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
120 Lytton Ave Ste 275
Pittsburgh, PA 15213

4815 Centre Avenue, Suite 106

2.73 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-2:00 PM • Sat: 8:30 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
4815 Centre Avenue, Suite 106
Pittsburgh, PA 15213

4765 Liberty Avenue,

2.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4765 Liberty Avenue,
Pittsburgh, PA 15224

300 Fleet St, 2nd Floor

3.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
300 Fleet St, 2nd Floor
Pittsburgh, PA 15220

5201 Baum Blvd

3.26 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
5201 Baum Blvd
Pittsburgh, PA 15224

4662 Broadway Road, Unit A-13

3.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4662 Broadway Road, Unit A-13
Allentown, PA 18104

2500 Baldwick Rd Ste 260

3.36 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:30 PM✓ Walk-in with Electronic Donor Pass
2500 Baldwick Rd Ste 260
Pittsburgh, PA 15205

2727 Murray Avenue,

3.85 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
2727 Murray Avenue,
Pittsburgh, PA 15217

969 Greentree Road, Second Floor

4.15 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-4:00 PM • Sat: 10:00 AM-11:30 AM✓ Walk-in with Electronic Donor Pass
969 Greentree Road, Second Floor
Pittsburgh, PA 15220

4225 Brownsville Rd, Shoppes At Brentwood

4.67 mi
🕑 Testing Hours: Mon–Fri: 9:30 AM-3:00 PM • Sat: 9:30 AM-11:30 AM✓ Walk-in with Electronic Donor Pass
4225 Brownsville Rd, Shoppes At Brentwood
Pittsburgh, PA 15227

642 Washington Road,

4.81 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
642 Washington Road,
Mt. Lebanon, PA 15228

3516 Saw Mill Run Blvd

4.89 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-6:00 PM • Sat: 9:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
3516 Saw Mill Run Blvd
Pittsburgh, PA 15227

1984 Greentree Rd

4.90 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1984 Greentree Rd
Pittsburgh, PA 15220

207 W 7th Ave

4.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
207 W 7th Ave
Homestead, PA 15120

1474 Route 51

5.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1474 Route 51
Jefferson Hills, PA 15025

633 Mount Royal Blvd

5.35 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-12:30 PM & 1:30 PM-4:00 PM✓ Walk-in with Electronic Donor Pass
633 Mount Royal Blvd
Pittsburgh, PA 15223

112 Third Ave

5.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
112 Third Ave
Carnegie, PA 15106

874 Butler St Ste 2

5.50 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
874 Butler St Ste 2
Pittsburgh, PA 15223

1100 Washington Avenue, Suite 114

5.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1100 Washington Avenue, Suite 114
Carnegie, PA 15106

4771 Mcknight Road,

5.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4771 Mcknight Road,
Pittsburgh, PA 15237

25 Heckel Rd

5.72 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
25 Heckel Rd
Mc Kees Rocks, PA 15136

15 Freeport Rd Ste 100

5.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
15 Freeport Rd Ste 100
Pittsburgh, PA 15215

50 Freeport Rd Ste 500

5.78 mi
🕑 Testing Hours: M,T,Th 8:00 AM-5:00 PM • W,F 8:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
50 Freeport Rd Ste 500
Aspinwall, PA 15215

50 Freeport Road, Unit 400

5.80 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
50 Freeport Road, Unit 400
Aspinwall, PA 15215

1769 Pine Hollow Rd

6.06 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM (Lunch: 12:30 PM - 01:00 PM)✓ Walk-in with Electronic Donor Pass
1769 Pine Hollow Rd
Mc Kees Rocks, PA 15136

4390 Campbells Run Rd

6.24 mi
🕑 Testing Hours: M-Th 8:00 AM-11:30 AM & 1:30 PM-3:30 PM • F 8:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
4390 Campbells Run Rd
Pittsburgh, PA 15205

1535 Washington Rd

6.47 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1535 Washington Rd
Pittsburgh, PA 15228

1691 Washington Rd

6.61 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-6:00 PM✓ Walk-in with Electronic Donor Pass
1691 Washington Rd
Mount Lebanon, PA 15228

1907 Lebanon Church Road, Suite 104

6.80 mi
🕑 Testing Hours: Mon–Fri: 9:30 AM-3:30 PM✓ Walk-in with Electronic Donor Pass
1907 Lebanon Church Road, Suite 104
West Mifflin, PA 15122

393 Vanadium Rd

6.82 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 11:30 AM, 01:30 PM - 03:30 PM
393 Vanadium Rd
Pittsburgh, PA 15243

2000 Oxford Dr Ste 110

7.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2000 Oxford Dr Ste 110
Bethel Park, PA 15102

1742 William Flynn Highway, Shaler Village

7.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1742 William Flynn Highway, Shaler Village
Glenshaw, PA 15116

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 40 DOT drug testing locations to serve you in and around Pittsburgh, PA.

At our Pittsburgh, PA DOT testing centers and in nearby areas, we provide a comprehensive suite of services including DOT Drug and Alcohol Testing, DOT Physicals, and Random pool and Consortium enrollment. Employers, independent operators, and all DOT sectors complying with 49 CFR Part 40—such as FMCSA, FTA, FAA, USCG, FRA, and PHMSA—can access our services quickly, often the same day, with convenient locations near homes and offices.

Select your required DOT service or drug/alcohol test and find a nearby facility. We cater to individuals, employees, or others through a smooth, efficient system that simplifies arranging DOT drug tests in Pittsburgh. Just choose and go!

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Pittsburgh, Pennsylvania DOT Services

DOT Drug Testing & Services Available in Pittsburgh, PA:

Our DOT services in Pittsburgh, PA encompass a broad spectrum of testing for employees and operators. Accredited Drug Testing ensures compliance through reliable drug and alcohol testing services, contributing to a safe and efficient work environment.

DOT Services in Pittsburgh, PA

DOT regulations in Pittsburgh, PA prioritize safety through mandatory drug and alcohol screening for employees, ensuring a drug-free workplace. These regulations help maintain the well-being of employees and the public.

Safety-sensitive positions in Pittsburgh, Pennsylvania undergo strict random testing under DOT regulations to keep incidents at bay. Each employee's adherence ensures collective safety.

Training sessions in Pittsburgh help employees grasp DOT compliance standards concerning drug and alcohol regulations, bolstering safety protocols across all affected industries.

Employee Assistance Programs (EAP) in Pittsburgh, PA support workers in meeting DOT regulations, promoting awareness, and accessing resources to maintain a drug-free workplace.

FMCSA Information

Pittsburgh, PA enforces FMCSA drug and alcohol regulations for commercial drivers, a crucial measure for maintaining public safety on the roads.

FMCSA rules in Pittsburgh include random testing to deter potential substance abuse, enhancing driver reliability and security.

USCG Information

Pittsburgh's USCG regulations ensure marine industry workers undergo drug and alcohol screenings, crucial for maritime safety and security.

To uphold safety, Pittsburgh USCG policies regularly test maritime employees, safeguarding both marine operations and personnel.

FAA Information

FAA mandates in Pittsburgh, PA require aviation employees to undergo regular drug and alcohol tests to secure air travel safety.

Pittsburgh's FAA rules enforce strict compliance for safety-sensitive aviation roles, minimizing risks related to substance abuse.

FRA Information

FRA testing protocols in Pittsburgh uphold train operation safety by ensuring that all rail employees adhere to drug-free mandates.

Railway safety in Pittsburgh is bolstered by FRA alcohol testing, preventing impairments that may compromise rail operations.

FTA Information

FTA testing policies in Pittsburgh, PA focus on ensuring the safety of public transportation by mandating routine drug and alcohol screening.

Pittsburgh public transit employees follow FTA rules, preserving public safety through compliance with comprehensive testing procedures.

PHMSA Information

PHMSA regulations in Pittsburgh safeguard hazardous material handlers through stringent drug and alcohol standards, critical for overall safety.

Random testing under PHMSA in Pittsburgh prevents substance misuse in safety-sensitive roles, ensuring operational integrity.

Scheduling in Pittsburgh, PA

Looking to arrange a DOT drug or alcohol test in Pittsburgh, PA? Call (800) 221-4291 or opt for online scheduling via our quick registration system. Select your test and complete the necessary registration swiftly to proceed smoothly to a nearby center.

By providing your zip code, we connect you with the nearest Pittsburgh testing centers offering your chosen test type. Easy email instructions will guide you—visit without appointments in most cases, but registration and payment need completing first.

Accredited Drug Testing assures accuracy through our SAMHSA-certified labs, where results are verified by licensed physicians acting as Medical Review Officers (MRO).

Apart from Pittsburgh, PA, our vast network extends to additional centers across the metropolitan region and neighboring cities, ensuring ample testing accessibility.

Be it travel or leisure, our services remain accessible with over centers nationwide. We schedule your testing everywhere, anytime, ensuring seamless and hassle-free service.

Pennsylvania Dept. of Transportation Updates

DUI Statistics in Pittsburgh, PA

Driving under the influence (DUI) remains a significant concern for Pittsburgh, PA, located within Allegheny County. In recent years, Pennsylvania has implemented stringent measures to curb DUI offenses, yet challenges persist. Pittsburgh sees a proportion of DUI incidents relative to the overall state numbers, largely mirroring trends seen in other metropolitan areas of Pennsylvania. Efforts in Pittsburgh, a city known for its vibrant cultural scene and extensive transportation network, focus on education, sobriety checkpoints, and increased law enforcement. The Pennsylvania DOT, working with local police, has enhanced campaigns to deter impaired driving. Fatalities linked to DUI in Pittsburgh underscore the importance of continued intervention and community involvement in DUI prevention strategies across Pennsylvania.

Drug-Involved Accidents in Pittsburgh, PA

Drug-involved accidents in Pittsburgh, PA, reflect a growing concern for Allegheny County's transportation infrastructure. Pennsylvania's Department of Transportation (PennDOT) has noted a trend of increasing accidents where substances other than alcohol are involved. Pittsburgh, with its large urban populace and varied road conditions, sees a share of these incidents as part of the broader state statistics. Drug involvement in traffic accidents has prompted discussions on public health and safety, highlighting the need for comprehensive strategies addressing both prevention and response. Various initiatives, including awareness programs and better training for law enforcement, have been launched in Pennsylvania to mitigate these types of accidents. Furthermore, collaboration with healthcare providers and community organizations across Pennsylvania and specifically in Pittsburgh aims to reduce such incidents.

Marijuana-Related Accidents in Pittsburgh, PA

Pittsburgh, PA, faces challenges with marijuana-related traffic incidents, reflecting a trend seen across Pennsylvania. Following legislative changes concerning marijuana use, discussions around road safety have intensified. In Pittsburgh, studies and statistics from PennDOT reveal the complexity of marijuana's impact on driving abilities, contributing to accidents within the city and Allegheny County. The city's law enforcement agencies, in alignment with Pennsylvania's broader drug-related driving policies, strive to educate the public on the risks associated with driving under the influence of marijuana. Efforts include increased training for officers to better detect impairment and outreach programs targeting both young drivers and the general public. These measures are vital to enhance road safety in Pittsburgh, a key urban center in Pennsylvania with dense traffic patterns.

Pittsburgh Drug Testing

Quickly find trusted local drug testing centers in Pittsburgh, PA — fast, convenient, and reliable every time!

Pittsburgh DOT Drug Testing

Quickly find a local DOT drug testing center in Pittsburgh, PA — fast, reliable, convenient nationwide service!

Pittsburgh DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Pittsburgh, PA.

Pittsburgh Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Pittsburgh, PA.v

Pittsburgh Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Pittsburgh, PA locations—results you can trust, every time!

Pittsburgh Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Pittsburgh, PA.

Pittsburgh Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Pittsburgh, PA.

Pittsburgh 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Pittsburgh, PA employers and individuals nationwide.

Pittsburgh 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Pittsburgh, PA workplace and personal testing needs.

Pittsburgh On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Pittsburgh, PA workplace compliant.

Pittsburgh DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Pittsburgh, PA drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291