DOT Drug Testing - Charlottesville, VA

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 38 testing centers in the Charlottesville area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

38 DOT Drug-Testing Centers in Charlottesville

Minutes Away, Test Today

1910 Arlington Blvd

1.45 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1910 Arlington Blvd
Charlottesville, VA 22903

125 Riverbend Dr Ste 3

1.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
125 Riverbend Dr Ste 3
Charlottesville, VA 22911

500 Ray C Hunt Dr

1.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
500 Ray C Hunt Dr
Charlottesville, VA 22903

320 Winding River Lane, Suite 104

2.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
320 Winding River Lane, Suite 104
Charlottesville, VA 22911

1149 Seminole Trl

2.56 mi
🕑 Testing Hours: Mon–Sat: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
1149 Seminole Trl
Charlottesville, VA 22901

500 Martha Jefferson Dr

2.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
500 Martha Jefferson Dr
Charlottesville, VA 22911

600 Peter Jefferson Pkwy 110

2.76 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
600 Peter Jefferson Pkwy 110
Charlottesville, VA 22911

260 Pantops Ctr

3.01 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
260 Pantops Ctr
Charlottesville, VA 22911

Po Box 220 Highway 15 South

16.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Po Box 220 Highway 15 South
New Canton, VA 23123

201 Lew Dewitt Blvd Outpatient Center

21.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
201 Lew Dewitt Blvd Outpatient Center
Waynesboro, VA 22980

428 S Magnolia Ave

22.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
428 S Magnolia Ave
Waynesboro, VA 22980

57 N Medical Park Dr Ste 101

27.54 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
57 N Medical Park Dr Ste 101
Fishersville, VA 22939

1371 Lee Highway

30.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1371 Lee Highway
Fort Defiance, VA 24437

350 Lee Hwy

30.65 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
350 Lee Hwy
Verona, VA 24482

1209 Richmond Ave

30.93 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
1209 Richmond Ave
Staunton, VA 24401

1555 Commerce Rd Ste C

31.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1555 Commerce Rd Ste C
Verona, VA 24482

422 Commerce Rd

32.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
422 Commerce Rd
Staunton, VA 24401

851 Statler Blvd

32.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
851 Statler Blvd
Staunton, VA 24401

42 Lambert St

32.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
42 Lambert St
Staunton, VA 24401

4038 Thomas Nelson Hwy

32.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4038 Thomas Nelson Hwy
Arrington, VA 22922

755a Mlk Jr Way

33.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
755a Mlk Jr Way
Harrisonburg, VA 22801

2505 Evelyn Byrd Ave Ste B

33.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2505 Evelyn Byrd Ave Ste B
Harrisonburg, VA 22801

1840 E Market St

33.74 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
1840 E Market St
Harrisonburg, VA 22801

420 Neff Avenue

33.74 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 03:00 PM
420 Neff Avenue
Harrisonburg, VA 22801

119 University Blvd Ste B

33.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
119 University Blvd Ste B
Harrisonburg, VA 22801

1790 E Market St Ste 64b

33.79 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1790 E Market St Ste 64b
Harrisonburg, VA 22801

1012 Reservoir St Ste A

34.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1012 Reservoir St Ste A
Harrisonburg, VA 22801

590c E Market St

35.47 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
590c E Market St
Harrisonburg, VA 22801

1765 S High St

35.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1765 S High St
Harrisonburg, VA 22801

100 Spottswood Dr

38.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
100 Spottswood Dr
Lexington, VA 24450

767 Madison Rd Ste 101

39.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
767 Madison Rd Ste 101
Culpeper, VA 22701

501 Sunset Ln

39.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
501 Sunset Ln
Culpeper, VA 22701

1200 Sunset Ln Ste 2210

39.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1200 Sunset Ln Ste 2210
Culpeper, VA 22701

1758 Anderson Hwy

39.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1758 Anderson Hwy
Cumberland, VA 23040

60 Commerce Park Dr

39.61 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
60 Commerce Park Dr
Raphine, VA 24472

1420 S Main St

40.18 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM • Sat:,Sun: 8:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
1420 S Main St
Culpeper, VA 22701

302 E Davis St

40.76 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
302 E Davis St
Culpeper, VA 22701

200 Memorial Dr

43.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
200 Memorial Dr
Luray, VA 22835

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 38 DOT drug testing locations to serve you in and around Charlottesville, VA.

Located in Charlottesville, VA and nearby areas, our network offers a range of DOT testing services including drug and alcohol tests, physicals, random pools, MIS, MVR, and driver qualification reports. We serve employers, individual operators, and all DOT sectors under 49 CFR Part 40 like FMCSA and more. Our centers are conveniently close to your location with same-day services typically available.

Select the desired DOT service or test and find a nearby center—testing caters to you, employees, or other individuals. Our efficient process facilitates effortless DOT drug testing in Charlottesville.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Charlottesville, Virginia DOT Services

DOT Drug Testing & Services Available in Charlottesville, VA:

In Charlottesville, VA, we offer comprehensive DOT services including drug and alcohol testing to ensure your team meets 49 CFR Part 40 standards efficiently via Accredited Drug Testing’s extensive network.

DOT Services in Charlottesville, VA

DOT regulations regarding drug and alcohol screening are critical for ensuring public safety, especially in Charlottesville, VA. Compliance with these regulations helps maintain a drug-free workplace and enhances safety for all employees involved.

Employers in Charlottesville, Virginia are required to conduct regular screenings under 49 CFR Part 40 to prevent drug and alcohol misuse in safety-sensitive positions. This ensures safe operability in various transport modes.

Random screenings form a considerable part of the DOT’s policy in Charlottesville, VA. These are unannounced and ensure that employees adhere to safety standards at all times, mitigating potential risks.

Annual training for supervisors in Charlottesville, Virginia includes recognizing signs of drug or alcohol influence, further bolstering the safety culture within transport operations.

Accredited Drug Testing aids employers in Charlottesville, VA by providing comprehensive testing services and ensuring their complete alignment with DOT regulations for employee safety.

FMCSA Information

For FMCSA compliance in Charlottesville, VA, regular drug and alcohol tests ensure safety-sensitive employees maintain a drug-free work environment as mandated.

Our Charlottesville, Virginia services include random and pre-employment testing as per FMCSA rules, ensuring public and workplace safety for transport sectors.

USCG Information

USCG regulations in Charlottesville, VA safeguard marine operations by implementing stringent drug and alcohol policies.

Adherence to USCG rules in Charlottesville, Virginia ensures maritime safety through comprehensive screenings and rigorous compliance checks.

FAA Information

FAA regulations in Charlottesville, VA demand regular screening of airline personnel. Accredited Drug Testing facilitates compliance with these requirements to enhance airline safety.

Drug and alcohol testing for FAA sectors in Charlottesville, Virginia involves periodic assessments and adherence to FAA protocols ensuring secure air travel.

FRA Information

FRA testing rules in Charlottesville, VA are crucial for maintaining the integrity of rail transport by preventing impairment on the job.

Charlottesville, Virginia follows FRA's structured testing programs to foster a safe and efficient rail system, preventing substance abuse-related incidents.

FTA Information

FTA's regulations in Charlottesville, VA focus on public transportation safety by enforcing drug and alcohol screening for all transit employees.

FTA guidelines in Charlottesville, Virginia are designed to maintain a drug-free environment within public transportation, safeguarding passengers and staff alike.

PHMSA Information

PHMSA drug testing in Charlottesville, VA focuses on preventing substance abuse in pipeline and hazardous material industries to protect the environment and public.

Compliance with PHMSA regulations in Charlottesville, Virginia includes random tests and thorough background checks to uphold safety standards.

Scheduling in Charlottesville, VA

To book a DOT drug or alcohol test in Charlottesville, VA, call our scheduling line at (800) 221-4291 or use our online system to register by selecting your test and filling in the necessary information. Registration is mandatory before visiting the center.

The zip code entered will locate the nearest DOT testing center in Charlottesville, VA. You will receive a donor pass with center details via email. Carry this form physically or digitally. Appointments are often unnecessary, though registration information and payment are required upfront.

All test results are verified by our SAMHSA-certified laboratories and reviewed by our in-house licensed Medical Review Officers (MRO).

Alongside DOT drug and alcohol testing in Charlottesville, VA, Accredited Drug Testing offers numerous testing centers across the metropolitan area and nearby cities.

Whether traveling or on vacation, Accredited Drug Testing boasts testing locations nationwide, ensuring testing can be scheduled anywhere, anytime.

Virginia Dept. of Transportation Updates

Charlottesville, VA DUI Statistics

The issue of driving under the influence (DUI) continues to be a significant concern for Charlottesville, VA, and the surrounding Albemarle County. In recent years, various initiatives have been put in place to combat DUI incidents. The Virginia Department of Transportation (VDOT) works closely with local law enforcement and community organizations to implement safety campaigns and educational programs aimed at reducing DUI occurrences. Annual reports indicate a decline in DUI-related accidents, but the figures still highlight the need for continuous efforts. The percentage of DUI arrests compared to total traffic incidents showcases a varying trend, demanding adaptive strategies to address underlying causes. Additionally, repeat offenders remain a focal point of attention. The community continues to promote awareness on the legal and personal consequences of impaired driving. Continuous community engagement and policy updates are crucial to support VDOT's mission to ensure safer roads in both Charlottesville, VA, and Albemarle County.

Charlottesville, VA Drug-Involved Accidents

Drug-involved accidents in Charlottesville, VA, pose a persistent challenge to transportation safety within the city and Albemarle County. According to recent data gathered by the Virginia Department of Transportation (VDOT) in collaboration with local agencies, a significant number of vehicle accidents are attributed to drug impairment. These incidents often involve both prescription medications and illegal substances, calling for a multifaceted approach to curb their impact. Strategies include increasing roadside drug testing capabilities and developing specialized training programs for first responders to manage drug-related traffic incidents effectively. Education campaigns aimed at informing residents about the dangers of driving under drug influence, particularly targeting young adults, have been initiated. Despite these efforts, the complexity stemming from various drugs emphasizes the need for continued research and policy adjustments. Addressing these accidents remains a pivotal part of VDOT's broader strategy to enhance public safety on Virginia’s roads.

Marijuana-Related Accidents in Charlottesville, VA

As marijuana legalization discussions continue across the United States, Charlottesville, VA, and Albemarle County deal with the repercussions on road safety. The Virginia Department of Transportation (VDOT) closely monitors marijuana-related accidents to understand and mitigate their effects. Although marijuana use is recognized for medical benefits, its impact on driving performance raises safety concerns. Recent studies indicate an increase in accidents where marijuana was a contributing factor, prompting VDOT to collaborate with local health departments and law enforcement. Efforts include awareness campaigns about responsible use and law enforcement training to recognize marijuana impairment in drivers. The intersection of marijuana use and driving is complex, affected by factors like the potency of marijuana, user tolerance, and concurrent use of other substances. By understanding these dynamics, VDOT aims to refine policies and enhance public education to address this evolving road safety issue in Charlottesville, VA, and its vicinity.

Charlottesville Drug Testing

Quickly find trusted local drug testing centers in Charlottesville, VA — fast, convenient, and reliable every time!

Charlottesville DOT Drug Testing

Quickly find a local DOT drug testing center in Charlottesville, VA — fast, reliable, convenient nationwide service!

Charlottesville DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Charlottesville, VA.

Charlottesville Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Charlottesville, VA.v

Charlottesville Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Charlottesville, VA locations—results you can trust, every time!

Charlottesville Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Charlottesville, VA.

Charlottesville Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Charlottesville, VA.

Charlottesville 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Charlottesville, VA employers and individuals nationwide.

Charlottesville 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Charlottesville, VA workplace and personal testing needs.

Charlottesville On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Charlottesville, VA workplace compliant.

Charlottesville DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Charlottesville, VA drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291