DOT Drug Testing - Appleton, WI

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 32 testing centers in the Appleton area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

32 DOT Drug-Testing Centers in Appleton

Minutes Away, Test Today

1501 S Madison St

1.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1501 S Madison St
Appleton, WI 54915

2000 S Memorial Dr

1.24 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-12:00 PM & 12:30 PM-5:00 PM✓ Walk-in with Electronic Donor Pass
2000 S Memorial Dr
Appleton, WI 54915

1650 Tri Park Way Ste A Ste A

2.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1650 Tri Park Way Ste A Ste A
Appleton, WI 54914

2809 N Park Drive Ln

2.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2809 N Park Drive Ln
Appleton, WI 54911

1221 E Northland Ave

2.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1221 E Northland Ave
Appleton, WI 54911

4000 W Spencer St

2.52 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
4000 W Spencer St
Appleton, WI 54914

2535 Northern Rd Ste 3b

2.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2535 Northern Rd Ste 3b
Appleton, WI 54914

1186 Appleton Rd

2.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1186 Appleton Rd
Menasha, WI 54952

3315 N Ballard Rd Ste C

2.96 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3315 N Ballard Rd Ste C
Appleton, WI 54911

702 Eisenhower Dr

3.37 mi
🕑 Testing Hours: M 8:00 AM-3:00 PM • T,W,F 8:00 AM-12:00 PM • Th 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
702 Eisenhower Dr
Kimberly, WI 54136

190 Tayco St

4.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
190 Tayco St
Menasha, WI 54952

984 Winchester Rd

5.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
984 Winchester Rd
Neenah, WI 54956

130 2nd St

5.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
130 2nd St
Neenah, WI 54956

101 Main St

5.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
101 Main St
Neenah, WI 54956

2700 Crooks Ave

7.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2700 Crooks Ave
Kaukauna, WI 54130

1136 Westowne Dr

7.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1136 Westowne Dr
Neenah, WI 54956

600 Thilmany Rd

7.77 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
600 Thilmany Rd
Kaukauna, WI 54130

555 Quality Ct

12.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
555 Quality Ct
Wrightstown, WI 54180

405 Commercial St

16.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
405 Commercial St
Seymour, WI 54165

964 W Ryan St Ste B

17.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
964 W Ryan St Ste B
Brillion, WI 54110

708 W Ryan St

17.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
708 W Ryan St
Brillion, WI 54110

1405 Mill St

17.76 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1405 Mill St
New London, WI 54961

126 S Main St

17.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
126 S Main St
Seymour, WI 54165

1855 S Koeller St

19.41 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1855 S Koeller St
Oshkosh, WI 54902

855 N Westhaven Dr

19.45 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
855 N Westhaven Dr
Oshkosh, WI 54904

600 N Westhaven Dr

19.45 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
600 N Westhaven Dr
Oshkosh, WI 54904

500 S Oakwood Rd

19.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
500 S Oakwood Rd
Oshkosh, WI 54904

614 Memorial Dr

20.86 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
614 Memorial Dr
Chilton, WI 53014

1630 Commanche Ave

22.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1630 Commanche Ave
Green Bay, WI 54313

1881 Chicago St

22.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1881 Chicago St
De Pere, WI 54115

760 Pilgrim Way

23.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
760 Pilgrim Way
Green Bay, WI 54304

2502 S Ashland Ave

23.10 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2502 S Ashland Ave
Green Bay, WI 54304

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 32 DOT drug testing locations to serve you in and around Appleton, WI.

At our Appleton, WI facilities, DOT services such as drug and alcohol tests, physicals, consortium enrollment, MIS and MVR reports, and driver qualification are offered. Our testing centers cater to various DOT modes including FMCSA, FTA, FAA, USCG, FRA, and PHMSA, meeting 49 CFR Part 40 compliance. Conveniently located centers ensure rapid, same-day service opportunities in most scenarios.

Choose the required DOT service or drug/alcohol test for easy access. Testing is streamlined, catering to individual needs. Visit a nearby center for your own, employee, or another's testing requirements, making DOT drug testing near Appleton seamless.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Appleton, Wisconsin DOT Services

DOT Drug Testing & Services Available in Appleton, WI:

Accredited Drug Testing in Appleton, WI offers a robust range of DOT services, including drug and alcohol testing tailored to meet federal compliance standards for individuals and employers alike.

DOT Services in Appleton, WI

DOT regulations mandate rigorous drug and alcohol tests to ensure the safety of all transportation employees in Appleton, WI. Maintaining safety is paramount, and compliance with the regulations ensures the wellbeing of everyone involved.

Employee education is crucial, and in Appleton, Wisconsin, training programs are available to reinforce understanding of these important safety measures under DOT standards.

Regular testing and readiness checks in Appleton, WI, ensure compliance and safety maintain integrity under ever-present DOT regulations.

Appleton's transportation sector benefits from stringent adherence to DOT standards, ensuring a reliable and secure travel experience for the community.

FMCSA Information

FMCSA rules in Appleton, WI require stringent drug and alcohol testing for commercial drivers to enhance safety and reliability on the roads.

Ensuring public safety, Appleton's FMCSA regulations support a drug-free transportation workforce through strict compliance checks.

USCG Information

USCG regulations in Appleton, WI require marine operator testing to prevent substance-related incidents on waterways.

In Appleton, WI, USCG maintains stringent drug policies promoting marine safety and environmental protection.

FAA Information

FAA testing regulations in Appleton, WI ensure pilots and crew remain drug-free, promoting aviation safety and operational reliability.

Through stringent FAA guidelines, Appleton, WI upholds a zero-tolerance stance on drug use in aviation sectors.

FRA Information

With FRA regulations in Appleton, WI, rail industry workers undergo routine drug and alcohol testing to ensure public safety.

Appleton, WI's railroads adhere to FRA standards, with regular testing ensuring a drug-free workforce for secure travel.

FTA Information

FTA regulations in Appleton, WI necessitate drug and alcohol testing for public transit employees, maintaining commuter safety.

In Appleton, WI, FTA's dedication to reducing substance misuse impacts positively on public transportation safety.

PHMSA Information

PHMSA regulations in Appleton, WI safeguard transportation with mandated drug and alcohol testing to prevent substance abuse.

In Appleton, WI, PHMSA's drug policies enforce transportation safety by screening employees involved in hazardous materials handling.

Scheduling in Appleton, WI

To arrange a DOT drug or alcohol test in Appleton, WI, contact us at (800) 221-4291 or use our online system. Select and register for your test, ensuring registration completion before visiting the center.

Your zip code helps us find the nearest Appleton testing site. Receive a donor pass with center details via email and present it upon arrival. Appointments aren't usually needed, but registration and payment are required upfront.

Our SAMHSA-accredited labs ensure accurate results, verified by licensed physician Medical Review Officers (MROs) within our team.

Beyond Appleton, WI, Accredited Drug Testing extends its services to various metropolitan and surrounding locations, enhancing accessibility.

Whether traveling or at home, rest easy knowing Accredited Drug Testing's nationwide network can schedule any test, anytime, including beyond Appleton, WI.

Wisconsin Dept. of Transportation Updates

DUI Statistics in Appleton, WI

In Appleton, WI, and the surrounding County of Outagamie, DUI (Driving Under the Influence) incidents are a significant concern for the Department of Transportation (DOT) and law enforcement agencies. The state of Wisconsin reports that DUI cases contribute to a substantial number of traffic incidents each year. In Appleton, the local authorities work in conjunction with the Wisconsin DOT to implement rigorous DUI checkpoints and public awareness campaigns to address and reduce the number of alcohol-impaired drivers on the roads. Through collaborations with local communities, these initiatives have been somewhat successful, yet tackling drunk driving remains a persistent challenge. The DOT continues to prioritize projects and programs aimed at reducing DUI-related accidents.

Drug-Involved Accidents in Appleton, WI

Appleton, WI, along with Outagamie County, experiences a variety of drug-involved traffic accidents, a trend mirrored in several parts of Wisconsin. The rising concern for the Wisconsin DOT is the increase in accidents caused by drivers operating vehicles under the influence of illicit substances other than alcohol. In Outagamie County, there is an observed increase in the misuse of prescription medications contributing to these statistics. These incidents often require collaborative efforts between the county's law enforcement and the Wisconsin DOT to monitor trends, promote safe driving habits, and educate the public. Campaigns focused on preventing drug-impaired driving are a critical part of the strategy to keep the roads in and around Appleton safe.

Marijuana-Related Accidents in Appleton, WI

In Appleton, WI, and the wider Outagamie County, the issue of marijuana-related traffic accidents is an ongoing issue for both the local authorities and the Wisconsin DOT. While marijuana use has seen legislative changes across the United States, including bordering states, its impact on road safety is closely watched. In Wisconsin, and particularly in cities like Appleton, there are efforts to comprehend the extent to which marijuana contributes to impaired driving incidents. The DOT collaborates with educational institutions and law enforcement to curb these incidents through increased awareness and enforcement of the state's impaired driving laws. Initiatives like educational campaigns and enhanced roadside testing aim to address the impact of marijuana on driving skills and safety.

Appleton Drug Testing

Quickly find trusted local drug testing centers in Appleton, WI — fast, convenient, and reliable every time!

Appleton DOT Drug Testing

Quickly find a local DOT drug testing center in Appleton, WI — fast, reliable, convenient nationwide service!

Appleton DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Appleton, WI.

Appleton Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Appleton, WI.v

Appleton Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Appleton, WI locations—results you can trust, every time!

Appleton Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Appleton, WI.

Appleton Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Appleton, WI.

Appleton 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Appleton, WI employers and individuals nationwide.

Appleton 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Appleton, WI workplace and personal testing needs.

Appleton On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Appleton, WI workplace compliant.

Appleton DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Appleton, WI drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

Super Helpful

Detricia was super helpful in making sure that the drug test is sent my worker for was the right one I needed for my company to be compliant with my customers drug test requirements.

Kevin Howell - 12/19/2024

Very professional and very quick

Very professional and very quick service to schedule appointments! I am pleased with the services and would definitely recommend using them for any drug testing needs!

Carrie Feerick - 4/19/2025

Quick, knowledgeable and courteous

Quick, knowledgeable and courteous Janelle worked diligently to support me. I am recommending their services to anyone looking.

Greensboro Joseph - 11/19/2024


(800) 221-4291