Drug Testing Locations - Nashville, TN

Employment, DOT, Court Ordered, Personal
Urine| Hair Follicle | Oral Fluid | Blood | ETG

34 Drug-Testing Centers in Nashville

Minutes Away, Test Today

3441 Dickerson Pike Outpatient Lab3.28 miles

3441 Dickerson Pike Outpatient Lab
Nashville, TN 37207

3638 Dickerson Pike Ste 2044.55 miles

3638 Dickerson Pike Ste 204
Nashville, TN 37207

1209 S Graycroft Ave5.66 miles

1209 S Graycroft Ave
Madison, TN 37115

360 Deaderick St5.76 miles

360 Deaderick St
Nashville, TN 37201

315 14th Ave5.84 miles

315 14th Ave
Nashville, TN 37203

1719 Gallatin Ave5.89 miles

1719 Gallatin Ave
Nashville, TN 37206

1916 Patterson St Ste 103,5.90 miles

1916 Patterson St Ste 103,
Nashville, TN 37203

300 20th Ave N5.99 miles

300 20th Ave N
Nashville, TN 37203

2010 Church St 1st Fl6.06 miles

2010 Church St 1st Fl
Nashville, TN 37236

602 Gallatin Ave6.08 miles

602 Gallatin Ave
Nashville, TN 37206

339 White Bridge Pike6.30 miles

339 White Bridge Pike
Nashville, TN 37209

114a 29th Ave N6.46 miles

114a 29th Ave N
Nashville, TN 37203

3404 W End Ave Ste 1016.81 miles

3404 W End Ave Ste 101
Nashville, TN 37203

6606 Charlotte Pike Ste 1047.23 miles

6606 Charlotte Pike Ste 104
Nashville, TN 37209

2800 Opryland Drive7.89 miles

2800 Opryland Drive
Nashville, TN 37214

1616 Gallatin Pike N8.35 miles

1616 Gallatin Pike N
Madison, TN 37115

1719 Gallatin Pike N8.50 miles

1719 Gallatin Pike N
Madison, TN 37115

1425 Elm Hill Pike8.82 miles

1425 Elm Hill Pike
Nashville, TN 37210

2001 Glen Echo Rd8.99 miles

2001 Glen Echo Rd
Nashville, TN 37215

1000 Northchase Dr Ste 2159.09 miles

1000 Northchase Dr Ste 215
Goodlettsville, TN 37072

2372 Lebanon Pike9.66 miles

2372 Lebanon Pike
Nashville, TN 37214

2531 Elm Hill Pike10.43 miles

2531 Elm Hill Pike
Nashville, TN 37214

Po Box 14029410.49 miles

Po Box 140294
Nashville, TN 37214

4300 Sidco Dr10.73 miles

4300 Sidco Dr
Nashville, TN 37204

3656 Trousdale Dr Ste 10910.80 miles

3656 Trousdale Dr Ste 109
Nashville, TN 37204

2740 Old Elm Hill Pike Ste 103a10.96 miles

2740 Old Elm Hill Pike Ste 103a
Nashville, TN 37214

519 Donelson Pike Ste 10711.25 miles

519 Donelson Pike Ste 107
Nashville, TN 37214

3055 Lebanon Pike Ste 100011.34 miles

3055 Lebanon Pike Ste 1000
Nashville, TN 37214

2267 Jackson Downs Blvd11.62 miles

2267 Jackson Downs Blvd
Nashville, TN 37214

7643 Highway 70 S11.77 miles

7643 Highway 70 S
Nashville, TN 37221

501 Metroplex Dr Ste 30612.40 miles

501 Metroplex Dr Ste 306
Nashville, TN 37211

391 Wallace Rd12.61 miles

391 Wallace Rd
Nashville, TN 37211

5225 Old Hickory Blvd Ste 20512.82 miles

5225 Old Hickory Blvd Ste 205
Hermitage, TN 37076

1645 Murfreesboro Pike Ste H12.98 miles

1645 Murfreesboro Pike Ste H
Nashville, TN 37217

Employment, DOT, Court Ordered, Probation, Family

Accredited Drug Testing provides a wide range of drug and alcohol testing services at our 34 testing locations around Nashville, Tennessee. Our offerings include both DOT and non-DOT urine tests, breathalyzer tests, EtG alcohol testing, and hair drug analysis, catering to individuals, companies, and legal requirements. In Nashville, TN, rapid result testing and SAMSA certified laboratory analyses are available with same-day service in most cases. Our Nashville centers are conveniently located close to your home or office. We also offer Occupational Health Testing, Clinical Testing, and conduct Background Checks.

Contact us at (800) 221-4291 or register online. Choose a test and location that suits you—whether it's for personal reasons, for your employees, or someone else. Scheduling is quick and easy; connect with our scheduling team or arrange your test online anytime. Our efficient process makes organizing drug testing near Nashville smooth and straightforward.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Nashville, TN Drug Testing

Drug Testing Services Available at Our Nashville, TN Labs:

At our Nashville drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Drug Testing in Nashville, TN

Nashville, Tennessee Statistics

In 2019, Davidson County, where Nashville is located, reported over 600 drug overdoses.

In 2022, Nashville saw a 20% increase in opioid-related deaths compared to 2021.

Davidson County experienced over 300 drug-related arrests in 2021.

Nashville ranks among the top Tennessee cities for methamphetamine abuse.

In 2020, Davidson County recorded over 1,500 emergency room visits due to drug overdoses.

Fentanyl was involved in 70% of the opioid-related overdose deaths in Nashville in 2021.

How does the body eliminate Drugs

Drug elimination is the sum of the processes of removing an administered drug from the body. In the pharmacokinetic ADME scheme (absorption, distribution, metabolism, and excretion), it is frequently considered to encompass both metabolism and excretion. Hydrophobic drugs, to be excreted, must undergo metabolic modification making them more polar. Hydrophilic drugs, on the other hand, can undergo excretion directly, without the need for metabolic changes to their molecular structures.

Although many sites of metabolism and excretion exist, the chief organ of metabolism is the liver, while the organ primarily tasked with excretion is the kidney. Any significant dysfunction in either organ can result in the accumulation of the drug or its metabolites in toxic concentrations.

A variety of other factors impact elimination — intrinsic drug properties, such as polarity, size, or pKa. Also other factors include genetic variation among individuals, disease states affecting other organs, and pathways involved in the way the drug distributes through the body, such as first-pass metabolism.

Issues of Concern

Drug elimination is the removal of an administered drug from the body. It is accomplished in two ways, either by excretion of an unmetabolized drug in its intact form or by metabolic biotransformation followed by excretion. While excretion is primarily carried out by the kidneys, other organ systems are involved as well. Similarly, the liver is the primary site of biotransformation, yet extrahepatic metabolism takes place in a variety of organ systems affecting multiple drugs.

Given the multiple organ systems and the variety of metabolic transformations present, drug elimination can entail a significant degree of complexity. Hydrophilic drugs are typically directly excreted by the kidneys, while hydrophobic drugs undergo biotransformation before excretion. The purpose here is twofold – biotransformation serves both detoxify the exogenous substances as well as to increase their hydrophilicity, ensuring their elimination via the kidneys.

Two broad metabolic pathways of hepatic drug transformation exist. Phase I is the direct modification of the target molecule, whereas phase II entails conjugation of the target to a polar molecule of low molecular weight. Phase I prepare the drug to enter phase II, but single-phase metabolism also exists.

Phase I involves oxidation, reduction, and hydrolysis of the exogenous molecule. These reactions are accomplished by hepatic microsomal enzymes, which reside in the smooth endoplasmic reticulum of the hepatocytes. Best known among them is the cytochrome P450 system, whose enzymes are predominantly involved in oxidative metabolism. Within the cytochrome P450 family (CYP), the enzyme responsible for the metabolism of more than 50% of existing drugs is the CYP3A4. Its activity encompasses various classes of medications, including opioids, immunosuppressants, antihistamines, and benzodiazepines. The enzymes can also be induced or inhibited by a variety of substances they interact with, including pharmaceuticals. The increase in metabolic activity with CYP induction results in a diminished activity of drugs targeted by that particular isoform. Conversely, CYP inhibition will result in increased drug plasma concentration, potentially leading toxicity. The CYP3A4 is induced by phenytoin, phenobarbital, and St. John's wort, while diltiazem, erythromycin, and grapefruit inhibit it. Caution is, therefore, necessary when administering CYP3A4-metabolized drugs in the presence of any of the inhibitors or inducers.

Phase II consists of covalent bonding of polar groups to nonpolar molecules to render them water-soluble and allow renal or biliary excretion. Target molecules enter phase II directly or via initial processing through phase I. A variety of polar adjuncts is transferred, including amino acids, glucuronic acid, glutathione, acetate, and sulfate. Glucuronidation is one of the major pathways of phase II biotransformation. The UDP-glucuronosyltransferase (UGT) enzyme family performs this activity. Typically, glucuronide derivatives possess less or no activity of the original drug, but in some cases, pharmacologically active compounds result. Morphine-6-glucuronide is a phase II metabolite of morphine with significant analgesic activity. As with the CYP enzymes, inducers, and inhibitors of phase II, enzymes exist and may influence the efficacy of drugs that rely on conjugation before excretion.

The first-pass effect is a feature of hepatic metabolism that also plays a role in the elimination of multiple drugs. Here, the enteric consumed drugs are exposed directly to the liver via the portal vein, where they undergo biotransformation before entering the systemic circulation. This activity reduces the bioavailability and needs to be factored into the dose administered to the patient. Intravenously administered drugs are not subject to the first-pass effect.

Extrahepatic drug metabolism takes place in the GI tract, kidneys, lungs, plasma, and skin.

Renal excretion completes the process of elimination that begins in the liver. Polar drugs or their metabolites get filtered in the kidneys and typically do not undergo reabsorption. They subsequently get excreted in the urine. Urinary pH has a significant impact on excretion, as drug ionization changes depending on the alkaline or acidic environment. Increased excretion occurs with weakly acidic drugs in basic urine and weakly basic drugs in acidic urine.

Excretion in the bile is another significant form of drug elimination. The liver can actively secrete ionized drugs with a molecular weight greater than 300 g/mol into bile, from where they reach the digestive tract and are either eliminated in feces or reabsorbed as part of the enterohepatic cycle.

Other pathways of excretion include the lungs, breast milk, sweat, saliva, and tears

Employers in Nashville, TN & Drug Testing Policies

In Nashville, employers are increasingly adopting strict drug testing policies given the high rates of substance abuse in Davidson County. Various companies conduct pre-employment, random, and post-incident drug testing to maintain a safe workplace.

The Society for Human Resource Management advises employers in Nashville to comply with state regulations for drug testing. Random testing is often employed in safety-sensitive positions such as transportation and healthcare.

Employers are also urged to offer Employee Assistance Programs (EAPs), providing support to those struggling with addiction. Partnering with local health agencies, many businesses offer resources for recovery and support as part of their inclusive workplace policies.

Government Efforts with Drug Problems in Nashville, TN

The city of Nashville in Davidson County has implemented several initiatives to combat drug abuse. The local government collaborates with state agencies to enhance treatment and recovery services. Tennessee Department of Mental Health and Substance Abuse Services is a key partner in these efforts.

Federal funding also supports Nashville's fight against drug problems, channeling resources into education and prevention programs. The Substance Abuse and Mental Health Services Administration frequently provides grants to support local programs aimed at reducing drug dependency.

Local Drug Busts & News in Nashville, TN

In recent years, Nashville has witnessed significant drug busts aimed at curbing the distribution of illegal substances. Law enforcement agencies in Davidson County actively pursue operations targeting narcotics trafficking.

One notable event was the multi-agency raid in 2021, which resulted in the seizure of large quantities of methamphetamine and fentanyl. This operation underscored the city's commitment to disrupting drug networks.

Community events such as the annual 'Drug-Free Nashville' rally aim to raise awareness and foster community engagement against drug abuse. These events often feature panel discussions, resource booths, and support groups to promote a drug-free society.

Occupational Health Services

Accredited Drug Testing offers fast, reliable employment screening services in Nashville, TN. Trusted by employers nationwide for accurate results and exceptional service.

Tennessee DOT/Non DOT Physicals

Tennessee Vision Tests

Tennessee Audiograms

Tennessee Respirator Fit Tests

Tennessee Lift Tests

Tennessee Background Checks

Resources & Citations

Tennessee Department of Mental Health and Substance Abuse Services

Tennessee Association of Drug Court Professionals

Tennessee Bureau of Investigation - Drug Diversion

Nashville Metro Public Health Department

Prevention Alliance of Tennessee

Tennessee Association of Alcohol, Drug & other Addiction Services

Drug Free Tennessee

Cumberland Heights

Renewal House

Nashville Drug Testing

Quickly find trusted local drug testing centers in Nashville, TN — fast, convenient, and reliable every time!

Nashville DOT Drug Testing

Quickly find a local DOT drug testing center in Nashville, TN — fast, reliable, convenient nationwide service!

Nashville DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Nashville, TN.

Nashville Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Nashville, TN.v

Nashville Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Nashville, TN locations—results you can trust, every time!

Nashville Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Nashville, TN.

Nashville Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Nashville, TN.

Nashville 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Nashville, TN employers and individuals nationwide.

Nashville 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Nashville, TN workplace and personal testing needs.

Nashville On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Nashville, TN workplace compliant.

Nashville DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Nashville, TN drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

Yes. Accredited Drug Testing maintains access to numerous collection sites nationwide, so in most cases a testing location is available close to a donor’s home, workplace, or job site. Same-day scheduling is often possible for both drug and alcohol testing needs.
Employers, DOT-regulated companies, attorneys, courts, probation departments, and private individuals all use Accredited Drug Testing locations. You do not need to be part of a large company to request testing — individuals can order testing directly.
Locations support urine drug tests, hair follicle drug tests, saliva/oral fluid drug tests, instant/rapid screening (where permitted), and evidential breath alcohol testing. Post-accident and reasonable suspicion testing are also available.
Yes. Accredited Drug Testing supports DOT-compliant urine drug testing and evidential breath alcohol testing following 49 CFR Part 40 requirements. This includes pre-employment, random, post-accident, reasonable suspicion, return-to-duty, and follow-up testing for safety-sensitive employees.
In many cases, yes. Same-day or next-day appointments can typically be arranged. This is especially important for urgent needs such as post-accident, reasonable suspicion, court deadlines, or probation compliance.
Yes. Results are released only to the authorized recipient — for example, an employer’s designated representative, the court or probation contact, or the individual who ordered the test. Results are handled securely and are suitable for policy enforcement and legal documentation.
Yes. Accredited Drug Testing offers on-site and mobile collections for employers. Mobile testing is useful for post-accident response, reasonable suspicion situations, high-volume hiring events, and remote job sites where sending employees offsite would cause downtime.
No. You do not need a physician's prescription to request most drug or alcohol tests. Individuals, employers, attorneys, and probation officers can schedule testing directly and send the donor to the assigned collection site.

Customer Reviews

Quick, knowledgeable and courteous

Quick, knowledgeable and courteous Janelle worked diligently to support me. I am recommending their services to anyone looking.

Greensboro Joseph - 11/19/2024

Sweet and helpful

Trish was amazing and got me through the sytem very fast and swift. I had a hard time hearing her a couple of times, but she was super sweet and helpful throughout the process. Highly recommend her!

Sophia Schutze - 6/19/2024

Reception is kind and helpful

Reception is kind and helpful , everything quick and simple , best urgent care

Yusuf Narmuradov - 11/19/2024


(800) 221-4291