✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Chester, PA

With 38 testing centers in the Chester area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

38 Drug-Testing Centers in Chester

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

510 E Gay Street

0.58 mi
🕑 Testing Hours: T,Th 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
510 E Gay Street
West Chester, PA 19382

600 E Marshall St,

0.75 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-2:00 PM • Sat: 7:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
600 E Marshall St,
West Chester, PA 19380

606 E Marshall St Ste 105

0.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
606 E Marshall St Ste 105
West Chester, PA 19380

915 Old Fern Hill Rd Ste 3

1.57 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 8:00 AM-6:00 PM✓ Walk-in with Electronic Donor Pass
915 Old Fern Hill Rd Ste 3
West Chester, PA 19380

1502 W Chester Pike,

4.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1502 W Chester Pike,
West Chester, PA 19382

183 W Lincoln Highway - Unit 8, Whiteland Towne Shopping Cente

5.05 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
183 W Lincoln Highway - Unit 8, Whiteland Towne Shopping Cente
Exton, PA 19341

308 E Lancaster Ave,

5.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
308 E Lancaster Ave,
Downingtown, PA 19335

150 E Pennsylvania Ave Ste 140

5.99 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-4:30 PM✓ Walk-in with Electronic Donor Pass
150 E Pennsylvania Ave Ste 140
Downingtown, PA 19335

99 Manor Ave Ste 2

6.10 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
99 Manor Ave Ste 2
Downingtown, PA 19335

102 Schubert Dr

6.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
102 Schubert Dr
Downingtown, PA 19335

257 W Uwchlan Ave Ste 220

6.19 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-1:00 PM & 1:30 PM-4:00 PM✓ Walk-in with Electronic Donor Pass
257 W Uwchlan Ave Ste 220
Downingtown, PA 19335

309 Lancaster Avenue,

6.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
309 Lancaster Avenue,
Frazer, PA 19355

625 N Pottstown Pike

6.39 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
625 N Pottstown Pike
Exton, PA 19341

736 Baltimore Pike, Suite 9

6.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
736 Baltimore Pike, Suite 9
Concordville, PA 19331

80 W Welsh Pool Rd Ste 102,

6.97 mi
🕑 Facility: Mon–Fri: 07:00 AM - 02:30 PM⚡ Drug Screens: Mon–Fri: 10:00 AM - 02:00 PM
80 W Welsh Pool Rd Ste 102,
Exton, PA 19341

80 W Welsh Pool Rd, Ste 205 N

6.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
80 W Welsh Pool Rd, Ste 205 N
Exton, PA 19341

80 W Welsh Pool Rd Ste 2005

7.04 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
80 W Welsh Pool Rd Ste 2005
Exton, PA 19341

School House Rd & Route 1, 132 Onix Drive

8.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
School House Rd & Route 1, 132 Onix Drive
Kennett Square, PA 19348

15 Industrial Blvd Ste, Suite A-101

8.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
15 Industrial Blvd Ste, Suite A-101
Paoli, PA 19301

3301 E Lincoln Hwy,

8.33 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-2:00 PM • Sat: 8:30 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
3301 E Lincoln Hwy,
Thorndale, PA 19372

11 Industrial Blvd

8.38 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11 Industrial Blvd
Paoli, PA 19301

1167a W Baltimore Pike,

9.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1167a W Baltimore Pike,
Media, PA 19063

1235 Horseshoe Pike,

9.96 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-1:30 PM✓ Walk-in with Electronic Donor Pass
1235 Horseshoe Pike,
Downingtown, PA 19335

201 Reeceville Rd

10.19 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-5:30 PM✓ Walk-in with Electronic Donor Pass
201 Reeceville Rd
Coatesville, PA 19320

830 W Cypress St

10.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
830 W Cypress St
Kennett Square, PA 19348

3600 Silverside Rd Ste B

11.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3600 Silverside Rd Ste B
Wilmington, DE 19810

3574 West Chester Pike,

11.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3574 West Chester Pike,
Newtown Square, PA 19073

1001 Chesterbrook Blvd

11.46 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1001 Chesterbrook Blvd
Berwyn, PA 19312

2722 Concord Pike

11.50 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
2722 Concord Pike
Wilmington, DE 19803

244 W Lincoln Hwy

11.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
244 W Lincoln Hwy
Coatesville, PA 19320

1403 Foulk Rd Ste 103,

11.88 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1403 Foulk Rd Ste 103,
Wilmington, DE 19803

2123 Concord Pike

12.23 mi
🕑 Facility: Mon–Fri: 06:00 AM - 02:00 PM⚡ Drug Screens: Mon–Fri: 06:00 AM - 12:30 PM
2123 Concord Pike
Wilmington, DE 19803

Phoenixville Plaza, 700 Nutt Road Unit 735

12.54 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Phoenixville Plaza, 700 Nutt Road Unit 735
Phoenixville, PA 19460

450 Cresson Blvd, Suite 3, Oaks Corporate Center

12.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
450 Cresson Blvd, Suite 3, Oaks Corporate Center
Phoenixville, PA 19460

2000 Sproul Rd Ste 102

13.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2000 Sproul Rd Ste 102
Broomall, PA 19008

920 W Sproul Rd Ste 110

13.70 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-1:00 PM • Sat: 9:30 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
920 W Sproul Rd Ste 110
Springfield, PA 19064

202 Lantana Dr

13.93 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
202 Lantana Dr
Hockessin, DE 19707

131 S State Rd

13.95 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-11:00 AM • Sat: 7:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
131 S State Rd
Springfield, PA 19064

Employment, DOT, Court Ordered, Probation, Family

Accredited Drug Testing in Chester, PA facilitates a thorough array of drug and alcohol tests at 38 centers. Our offerings include DOT and non-DOT urine drug screenings, breath alcohol checks, EtG tests, and hair follicle drug tests tailored for personal, workplace, and judicial requirements. With rapid result options and SAMSA certified lab evaluations, we provide prompt same-day services, and most Chester centers are conveniently located near you. We also specialize in Occupational Health, Clinical Testing, and conducting Background Verifications.

Dial (800) 221-4291 or visit us online to register. Choose the test and the closest center—whether it is for you, your employees, or someone else, the process is straightforward. Arranging a test is quick and hassle-free; contact our team or set up online testing anytime, 24/7. Our intuitive system makes securing drug tests near Chester incredibly easy.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Chester, PA

1

Order Online or Call

Select your test & search nearby Chester collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Chester, PA Drug Testing

Drug Testing Services Available at Our Chester, PA Labs:

At our Chester drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Pennsylvania Drug Testing Laws & Compliance in Chester

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Chester, PA adhering strictly to 43 P.S. § 951 et seq., federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
43 P.S. § 951 et seq.
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Prohibited
Workplace Rights
Zero-Tolerance Protected

Pennsylvania law supports employer zero-tolerance workplace drug policies, allowing pre-employment, safety-sensitive, and reasonable suspicion testing programs.

Under 43 P.S. § 951 et seq., employers in Chester may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Prohibited in Pennsylvania), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Chester, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Chester and Pennsylvania.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Chester, PA

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Chester to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Chester Neighborhoods Covered:

● Downtown Chester ● Central Business District ● North Chester Commercial Corridor ● South Chester Industrial Park ● East Chester Metro Area ● West Chester Trade Center ● Medical & Healthcare District ● Airport / Logistics Corridor

Major Employment & Commercial Hubs Served:

Chester Main Street Commercial District • Chester Regional Trade Center • County Industrial Parkway • Metro Healthcare & Occupational Medical Corridor

Located anywhere in Chester? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Chester

West Chester • Chatwood • West Goshen • Bradford Hills • Westtown • Milltown • Lenape • Goshenville

Chester, PA Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Chester.

Test Type & Local Panel Detection Window Common Use Cases in Chester Turnaround Time
5-Panel Urine Screen in Chester →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Chester →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Chester →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Chester →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Chester →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Chester →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Chester, PA

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Chester.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Chester facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Chester, PA

Need immediate walk-in service in Chester? View our Walk-In Drug Testing Near Me in Chester, PA facility guide or order an instant electronic donor pass online.

Chester Drug Testing

Quickly find trusted local drug testing centers in Chester, PA — fast, convenient, and reliable every time!

Chester DOT Drug Testing

Quickly find a local DOT drug testing center in Chester, PA — fast, reliable, convenient nationwide service!

Chester DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Chester, PA.

Chester Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Chester, PA.v

Chester Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Chester, PA locations—results you can trust, every time!

Chester Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Chester, PA.

Chester Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Chester, PA.

Chester 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Chester, PA employers and individuals nationwide.

Chester 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Chester, PA workplace and personal testing needs.

Chester On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Chester, PA workplace compliant.

Chester DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Chester, PA drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Pennsylvania, workplace drug testing is governed by statutory guidelines under 43 P.S. § 951 et seq.. Private employers generally operate under a Pennsylvania Medical Marijuana Act (35 P.S. § 10231.2103), permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Pennsylvania who implement a certified Drug-Free Workplace Program in compliance with 43 P.S. § 951 et seq. can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Pennsylvania state law compliance standards, state statutes prohibits discrimination against certified medical marijuana cardholders. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 38 certified collection sites in the Chester area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Chester, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

Excellent service!

Excellent service! Trish went above and beyond to find me a testing site as close to my home as possible. Results were faster than expected, as well. Thanks for your help, Trish and Accredited!

Es Hache - 4/13/2025

Reception is kind and helpful

Reception is kind and helpful , everything quick and simple , best urgent care

Yusuf Narmuradov - 11/19/2024

Fast and efficient service

Fast and efficient service for employers wanting to do pre employment drug screening that meets DOT requirements!!

Mary Thomas - 4/5/2025


(800) 221-4291