DOT Physical Exams - Holder, FL

Locations, All Cities, All States

FMCSA | FAA | USCG | FTA | FRA | PHMSA

With 40 testing centers in the Holder area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

40 DOT Drug-Testing Centers in Holder

Minutes Away, Test Today

1907 Hwy 44 W

8.38 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1907 Hwy 44 W
Inverness, FL 34453

6140 W Corporate Oaks Dr,

9.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6140 W Corporate Oaks Dr,
Crystal River, FL 34429

312 S. Line Ave., Ste B

10.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
312 S. Line Ave., Ste B
Inverness, FL 34453

210 S. Apopka Ave,

10.79 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-2:00 PM • Sat: 6:00 AM-10:00 AM✓ Walk-in with Electronic Donor Pass
210 S. Apopka Ave,
Inverness, FL 34452

9401 Sw Hwy 200,b1000,s1002

10.87 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9401 Sw Hwy 200,b1000,s1002
Ocala, FL 34481

580 Se 7th Ave

11.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
580 Se 7th Ave
Crystal River, FL 34429

8602 Sw State Road 200, 103rd Street Plaza, Suite J

13.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8602 Sw State Road 200, 103rd Street Plaza, Suite J
Ocala, FL 34481

3956 S Suncoast Blvd

14.66 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
3956 S Suncoast Blvd
Homosassa, FL 34448

6160 Sw Highway 200

15.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6160 Sw Highway 200
Ocala, FL 34476

659 Ne Hwy 19 Ste 1

16.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
659 Ne Hwy 19 Ste 1
Crystal River, FL 34429

11371 N Williams St Unit 4

16.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11371 N Williams St Unit 4
Dunnellon, FL 34429

2415 Sw College Rd

19.88 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
2415 Sw College Rd
Ocala, FL 34471

2685 Sw 32nd Place,

20.10 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2685 Sw 32nd Place,
Ocala, FL 34471

1109 Sw 10th St

22.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1109 Sw 10th St
Ocala, FL 34471

1834 Sw 1st Ave Ste 201

22.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1834 Sw 1st Ave Ste 201
Ocala, FL 34471

10762 Se Us Highway 441

22.36 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10762 Se Us Highway 441
Belleview, FL 34420

31 Sw 12th St

22.47 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
31 Sw 12th St
Ocala, FL 34471

1015 Se 17th St Ste 200

22.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1015 Se 17th St Ste 200
Ocala, FL 34471

2100 Se 17th St Ste 902

23.47 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2100 Se 17th St Ste 902
Ocala, FL 34471

1389 Se Us Hwy 301

25.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1389 Se Us Hwy 301
Sumterville, FL 33585

11962 County Road 101 Ste 402 The Villa

26.31 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11962 County Road 101 Ste 402 The Villa
Lady Lake, FL 32162

910 Old Camp Rd Bldg 110 Ste 112

27.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
910 Old Camp Rd Bldg 110 Ste 112
The Villages, FL 32162

17820 Se 109 Avenue, Suite 106a

27.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
17820 Se 109 Avenue, Suite 106a
Summerfield, FL 34491

117 W Belt Ave Ste A

27.77 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
117 W Belt Ave Ste A
Bushnell, FL 33513

8600a County Road 466. Ste A

28.05 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8600a County Road 466. Ste A
The Villages, FL 32162

17240 Cortez Blvd

29.45 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
17240 Cortez Blvd
Brooksville, FL 34601

20205 Cortez Blvd

29.65 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
20205 Cortez Blvd
Brooksville, FL 34601

12114 Cortez Blvd #205

30.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12114 Cortez Blvd #205
Brooksville, FL 34613

12220 Cortez Blvd

30.76 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12220 Cortez Blvd
Brooksville, FL 34613

12120 Cortez Blvd

30.83 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12120 Cortez Blvd
Brooksville, FL 34613

7007 Nightwalker Rd,

31.33 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-3:00 PM • Sat: 6:30 AM-10:30 AM✓ Walk-in with Electronic Donor Pass
7007 Nightwalker Rd,
Brooksville, FL 34613

805 E County Rd 466

31.65 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
805 E County Rd 466
Lady Lake, FL 32159

31075 Cortez Blvd

32.76 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
31075 Cortez Blvd
Brooksville, FL 34602

2500 Citrus Blvd

32.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2500 Citrus Blvd
Leesburg, FL 34748

4112 Mariner Blvd

33.76 mi
🕑 Testing Hours: Mon–Sat: 9:00 AM-9:00 PM • Sun: 10:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
4112 Mariner Blvd
Spring Hill, FL 34609

3037 Landover Blvd

34.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3037 Landover Blvd
Spring Hill, FL 34608

3041 Landover Blvd.,

34.22 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-2:00 PM • Sat: 6:00 AM-10:00 AM✓ Walk-in with Electronic Donor Pass
3041 Landover Blvd.,
Spring Hill, FL 34608

101 S 11th St Ste 2,

34.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
101 S 11th St Ste 2,
Leesburg, FL 34748

34498 Cortez Blvd

35.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
34498 Cortez Blvd
Ridge Manor, FL 33523

601 E Dixie Ave Ste 804

35.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
601 E Dixie Ave Ste 804
Leesburg, FL 34748

Holder, FL DOT Physical - What You Need to Know

A DOT physical is an in-depth health check required by the Department of Transportation for all commercial vehicle operators. The purpose of this exam is to confirm that drivers are in good physical, mental, and emotional health to handle large vehicles safely on public thoroughfares. Certified medical examiners, registered on the National Registry of Certified Medical Examiners, conduct this examination. A successful exam grants drivers a DOT medical certificate, essential for holding a valid CDL license as per FMCSA standards. If your regulation falls under another DOT agency and you need guidance, call us at 800-221-4291.

During your exam, the medical examiner will complete a thorough health assessment to determine if you meet the Federal Motor Carrier Safety Administration (FMCSA) medical standards. The process typically includes:

What Does a DOT Physical Include?

Health History Review
Vision Test

Hearing Test
Urinalysis

Vital Signs
Physical Examination

DOT Testing | DOT Breath Alcohol | DOT Consortium Services | MVR Reports Also Available


What is checked in a DOT Physical?

  • Review of health history and medication use
  • Eyesight test (corrective lenses accepted, minimum levels required)
  • Hearing test (whisper test or audiometry)
  • Blood pressure and pulse check
  • Physical evaluation (systems assessment)
  • Urine test (specific gravity, protein, glucose)

Upon successful completion, your Medical Examiner's Certificate (MEC) may be issued for up to 24 months, with the potential for shorter duration requiring specialist validation in Holder, MS.

Who needs a DOT Physical?

In Holder, TN, to maintain your commercial operations, a valid DOT medical certificate is mandatory if you:

  • Hold a CDL - class A, B, or C for interstate trade
  • Operate vehicles exceeding 10,001 lbs. GVWR in interstate trade
  • Carry over 8 passengers (commercial) or beyond 15 (non-commercial)
  • Haul hazardous materials needing placards as per DOT rules
  • Are employed by DOT-regulated bodies like FMCSA or similar entities requiring DOT medical qualifications

What to bring with you to a DOT Physical?

  • Government-issued photo ID
  • Corrective eyewear & prescription
  • List of current medications & dosages
  • CPAP compliance data (if relevant)
  • Specialist approvals (cardiology, sleep apnea, diabetes, etc.)
  • Recent A1C or blood pressure records if under monitoring
Holder, ID.

What happens if I fail a DOT Physical?

  • No Medical card will be issued
  • Temporary disqualification may occur
  • Referral for treatment to your main physician
  • FMCSA exceptions for some medical conditions
  • Employer notification
  • Next steps include resolving medical concerns and reattempting the exam

If you're in Holder, CO, address these issues and pursue the necessary steps to regain eligibility and fulfill the requirements as indicated for successful qualification.

What Will Fail a DOT Physical?

Medical Conditions That Can Fail a DOT Physical

  • Vision & Hearing: Vision worse than 20/40 in both eyes with correction, inability to identify traffic signal colors, or hearing loss obstructing hearing a forced whisper at a 5-foot range.
  • Blood Pressure & Heart Disease: Severe hypertension (≥180/110 mmHg), recent heart attack or stroke, untreated angina, or an implanted defibrillator lacking medical clearance.
  • Diabetes: Poorly managed diabetes leading to frequent low blood sugar events or complications like neuropathy or impaired vision that hinder safe driving.
  • Sleep Apnea & Respiratory Issues: Untreated obstructive sleep apnea causing daytime sleepiness or serious lung condition affecting oxygen intake.
  • Neurological Disorders: Epilepsy or seizure conditions (except those exempted), disorders leading to sudden unconsciousness, dizziness, or uncontrollable shaking.
  • Substance Abuse: Active illegal drug usage, alcohol dependency, or misuse of prescribed medicines that impair driving capabilities.
  • Psychiatric & Cognitive Disorders: Severe unmanaged psychiatric disorders (like schizophrenia or bipolar disorder with recent mood episodes) or cognitive decline impacting decision-making and reflexes.

Some problems might only result in temporary disqualification until medical clearance or proof of control (e.g., regulated blood pressure, diabetes care) is supplied. Conversely, certain conditions such as untreated seizure disorders or the presence of an implanted defibrillator are generally viewed as permanently disqualifying based on FMCSA guidelines.

Holder Drug Testing

Quickly find trusted local drug testing centers in Holder, FL — fast, convenient, and reliable every time!

Holder DOT Drug Testing

Quickly find a local DOT drug testing center in Holder, FL — fast, reliable, convenient nationwide service!

Holder DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Holder, FL.

Holder Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Holder, FL.v

Holder Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Holder, FL locations—results you can trust, every time!

Holder Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Holder, FL.

Holder Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Holder, FL.

Holder 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Holder, FL employers and individuals nationwide.

Holder 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Holder, FL workplace and personal testing needs.

Holder On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Holder, FL workplace compliant.

Holder DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Holder, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT physical typically includes a review of the driver’s health history and medications, vision screening, hearing assessment, vital signs (blood pressure, pulse), urinalysis (checking specific gravity, protein, glucose), and a full physical examination by a certified medical examiner. Upon passing, a Medical Examiner’s Certificate (DOT medical card) may be issued for up to 24 months.
You must have a valid DOT medical certificate if you: hold a commercial driver’s license (CDL) Class A, B or C for interstate commerce; operate a vehicle over 10,001 lbs GVWR in interstate commerce; transport more than eight passengers (for hire) or over fifteen passengers (not for hire); or transport hazardous materials requiring placarding under DOT regulations.
DOT physicals are offered at test centres in all cities and all states, through Accredited Drug Testing’s nationwide network. You can schedule by calling 1-800-221-4291.
You should bring a government-issued photo ID; glasses or contact lenses and the prescription; a list of medications and dosages; CPAP usage data if applicable; and any specialist clearance letters or physician documentation (for conditions like heart disease, sleep apnea, diabetes) to your DOT physical.
If you fail the DOT physical, you will not receive the medical card; you may be temporarily or permanently disqualified depending on the condition; you will need to address any medical issues (e.g., uncontrolled hypertension, untreated sleep apnea, recent myocardial infarction) and then be re-examined.

Customer Reviews

test was quick and easy

Company New Hire Employee Process for DOT Drug test was quick and easy. No need to make appt. All employee had to do is show prepaid qr code I sent to employee show ID and was set. Would Recommend.

Maria Lopez - 11/19/2024

Pretty Straightforward

It was a pretty straightforward process getting an appointment and making payment. Getting to the testing site was quick. I was in and out in no time.

Sadiat Ogunyemi - 12/19/2024

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Everything was great

Everything was great, the staff was very polite. Thank you.

Olga Petrova - 9/19/2024

Friendly and Knowledgeable

I reached out Accredited Drug Testing/Secure Screenings to partner for my drug testing business (Discovery Diagnostics Columbia SC) and the process was extremely easy. I worked with Sicilia who was amazing. She was friendly & knowledgeable, and answer all of my questions with ease.

Kendra Harley - 3/26/2025

Excellant Performance

The visit here is always the best . The place is always really clean. The employees are super courteous, very polite, and professional. This is the only drug lab I like to go do my drug and alcohol test. I would like to tell them thank you so much for thier excellent performance and job

Eli Gonzalez - 1/4/2025

Fantastic Experience

Fantastic experience from the beginning. Jocelyn was very helpful to me as someone who doesn’t have much experience in needing a test. They were very helpful and walked me through the entire process start to finish. Thank you ADT!

Riley Wilson - 2/18/2025

Fast and efficient service

Fast and efficient service for employers wanting to do pre employment drug screening that meets DOT requirements!!

Mary Thomas - 4/5/2025

Very professional and very quick

Very professional and very quick service to schedule appointments! I am pleased with the services and would definitely recommend using them for any drug testing needs!

Carrie Feerick - 4/19/2025

Smooth and Seamless

I use their service for new hire and DOT employee's. Spoke with Taisha Walker this morning, and she was very helpful. She made the process smooth and seamless.

Christina Galdos - 3/9/2025


(800) 221-4291