DOT Physical Exams - Lake City, FL

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With 34 testing centers in the Lake City area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

(800) 221-4291

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34 DOT Drug-Testing Centers in Lake City

Minutes Away, Test Today

325 E Baya Ave

0.54 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
325 E Baya Ave
Lake City, FL 32025
Call (800) 221-4291 Get Donor Pass

260 S Marion Ave Bldg B Ste 125

1.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
260 S Marion Ave Bldg B Ste 125
Lake City, FL 32025
Call (800) 221-4291 Get Donor Pass

265 Sw Malone Street,suite 105

1.77 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
265 Sw Malone Street,suite 105
Lake City, FL 32025
Call (800) 221-4291 Get Donor Pass

4134 W Us Highway 90

2.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4134 W Us Highway 90
Lake City, FL 32055
Call (800) 221-4291 Get Donor Pass

3824 W Us Highway 90

3.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3824 W Us Highway 90
Lake City, FL 32055
Call (800) 221-4291 Get Donor Pass

3824 E Us Highway 90

3.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3824 E Us Highway 90
Lake City, FL 32055
Call (800) 221-4291 Get Donor Pass

221 Sw Stonegate Terrace, Ste 107

4.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
221 Sw Stonegate Terrace, Ste 107
Lake City, FL 32024
Call (800) 221-4291 Get Donor Pass

4367 Nw American Ln

5.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4367 Nw American Ln
Lake City, FL 32055
Call (800) 221-4291 Get Donor Pass

128 Nw 137th Dr

14.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
128 Nw 137th Dr
Newberry, FL 32669
Call (800) 221-4291 Get Donor Pass

850 E Main St Attn Lab

18.18 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
850 E Main St Attn Lab
Lake Butler, FL 32054
Call (800) 221-4291 Get Donor Pass

1506 Ohio Ave S Ste 2

21.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1506 Ohio Ave S Ste 2
Live Oak, FL 32064
Call (800) 221-4291 Get Donor Pass

215 Howard St W

21.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
215 Howard St W
Live Oak, FL 32064
Call (800) 221-4291 Get Donor Pass

420 Ne Santa Fe Blvd

25.31 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
420 Ne Santa Fe Blvd
High Springs, FL 32643
Call (800) 221-4291 Get Donor Pass

14417 Nw 152nd Ln

28.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14417 Nw 152nd Ln
Alachua, FL 32615
Call (800) 221-4291 Get Donor Pass

3470 Nw 57th Trl

28.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3470 Nw 57th Trl
Bell, FL 32619
Call (800) 221-4291 Get Donor Pass

14819 Nw 140th St

28.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14819 Nw 140th St
Alachua, FL 32615
Call (800) 221-4291 Get Donor Pass

413 5th Ave Nw Ste 3

29.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
413 5th Ave Nw Ste 3
Jasper, FL 32052
Call (800) 221-4291 Get Donor Pass

12 W Macclenny Ave

31.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12 W Macclenny Ave
Macclenny, FL 32063
Call (800) 221-4291 Get Donor Pass

1437 S 6th St

31.46 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-6:00 PM✓ Walk-in with Electronic Donor Pass
1437 S 6th St
Macclenny, FL 32063
Call (800) 221-4291 Get Donor Pass

159 N 3rd St

31.72 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
159 N 3rd St
Macclenny, FL 32063
Call (800) 221-4291 Get Donor Pass

1550 S Water St

35.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1550 S Water St
Starke, FL 32091
Call (800) 221-4291 Get Donor Pass

345 W Madison St

35.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
345 W Madison St
Starke, FL 32091
Call (800) 221-4291 Get Donor Pass

3925 Nw 43rd St

37.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3925 Nw 43rd St
Gainesville, FL 32606
Call (800) 221-4291 Get Donor Pass

1131 Nw 64th Ter Ste A

38.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1131 Nw 64th Ter Ste A
Gainesville, FL 32605
Call (800) 221-4291 Get Donor Pass

812 Nw 57th St

39.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
812 Nw 57th St
Gainesville, FL 32605
Call (800) 221-4291 Get Donor Pass

4343 W Newberry Rd Ste 10

39.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4343 W Newberry Rd Ste 10
Gainesville, FL 32607
Call (800) 221-4291 Get Donor Pass

4881 Nw 8th Ave Ste 2a

39.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4881 Nw 8th Ave Ste 2a
Gainesville, FL 32605
Call (800) 221-4291 Get Donor Pass

6121 Nw 1st Pl

39.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6121 Nw 1st Pl
Gainesville, FL 32607
Call (800) 221-4291 Get Donor Pass

825 Northwest 23rd Ave., Unit 2

40.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
825 Northwest 23rd Ave., Unit 2
Gainesville, FL 32609
Call (800) 221-4291 Get Donor Pass

720 Sw 2nd Ave Ste 160a

41.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
720 Sw 2nd Ave Ste 160a
Gainesville, FL 32601
Call (800) 221-4291 Get Donor Pass

3581 Sw Archer Rd Units 30 And 40

43.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3581 Sw Archer Rd Units 30 And 40
Gainesville, FL 32608
Call (800) 221-4291 Get Donor Pass

1708 Blanding Blvd

48.80 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
1708 Blanding Blvd
Middleburg, FL 32068
Call (800) 221-4291 Get Donor Pass

1113 Nw 23rd Ave

49.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1113 Nw 23rd Ave
Chiefland, FL 32626
Call (800) 221-4291 Get Donor Pass

228 Ne Hancock Ave

49.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
228 Ne Hancock Ave
Madison, FL 32340
Call (800) 221-4291 Get Donor Pass

Lake City, FL DOT Physical - What You Need to Know

A comprehensive medical evaluation known as a DOT physical is necessary for all commercial motor vehicle drivers, mandated by the Department of Transportation (DOT). This examination checks if professional drivers are physically, mentally, and emotionally capable of safely operating large vehicles on public roads.

Conducted by a certified medical examiner (CME) listed on the National Registry of Certified Medical Examiners (NRCME), passing this examination allows drivers to obtain a DOT medical certificate, which is needed to keep a valid CDL license in accordance with FMCSA regulations. For guidance on the appropriate DOT physical, if regulated by another DOT agency, reach out to us at 800-221-4291.

During your exam, the medical examiner will complete a thorough health assessment to determine if you meet the Federal Motor Carrier Safety Administration (FMCSA) medical standards. The process typically includes:

What Does a DOT Physical Include?

Health History Review
Vision Test

Hearing Test
Urinalysis

Vital Signs
Physical Examination

DOT Testing | DOT Breath Alcohol | DOT Consortium Services | MVR Reports Also Available


What is checked in a DOT Physical?

  • Health history review and medication assessment
  • Vision screening (corrective lenses allowed; minimum standards apply)
  • Hearing assessment (forced whisper or audiometry)
  • Blood pressure and pulse rate
  • Physical examination (systems review)
  • Urinalysis (specific gravity, protein, glucose)

Upon successful completion, your Medical Examiner's Certificate (MEC) may be issued for up to 24 months. Some conditions may require shorter certification or specialist documentation

For more details, explore local guidelines in Lake City, HI.

Who needs a DOT Physical?

Having a valid DOT medical card is crucial if:

  • You possess a commercial driver's license (CDL) of class A, B, or C suitable for interstate commerce.
  • Your vehicle's GVWR exceeds 10,001 lbs and you conduct interstate business.
  • You transport more than 8 fare-paying passengers or above 15 if not taking any fare.
  • You move hazardous materials needing DOT-required placarding.
  • You are employed under any DOT-agency jurisdiction like FMCSA, FAA, FRA, FTA, or USCG requiring medical qualifications, operational in Lake City, OK.

DOT Physical Near Lake City, FL

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What to bring with you to a DOT Physical?

  • Government ID with photo
  • Eyeglasses/contact lenses and prescription
  • A list of all medications and their dosages
  • Data from CPAP usage (if applicable)
  • Documentation from specialists (cardiology, sleep apnea, diabetes)
  • Recent A1C or blood pressure logs if needed

Ensure you have everything necessary for a complete examination, especially in Lake City, FL.

What happens if I fail a DOT Physical?

  • No Medical card will be issued
  • Temporary disqualification
  • Referral for treatment to your primary care doctor
  • FMCSA exemptions for certain medical conditions
  • Employer Notification
  • Next Steps (address the medical issues and retake the exam)

Drivers in Lake City, WY should take note of these steps in the event of a failed exam.

What Will Fail a DOT Physical?

Medical Conditions That Can Fail a DOT Physical

  • Vision & Hearing: Vision must be no worse than 20/40 per eye with correction, and must distinguish traffic signal colors. Hearing must detect a forced whisper at 5 feet.
  • Blood Pressure & Heart Issues: Hypertension uncontrolled at above 180/110 mmHg, or recent cardiac events, including heart attack, stroke, or angina, may disqualify without clearance.
  • Diabetes: Unmanaged diabetes leading to frequent hypoglycemic events or nerve and vision complications can bar safe driving.
  • Sleep & Breathing Disorders: Untreated sleep apnea causing daytime sleepiness, or severe respiratory illnesses affecting oxygenation, are disqualifying.
  • Neurological Conditions: Epilepsy or conditions provoking sudden unconsciousness or uncontrolled tremors generally disqualify unless exempted.
  • Substance Concerns: Illegal drug use or alcohol dependence that prejudices safe driving are disqualifying.
  • Mental & Cognitive Health: Severe uncontrolled mental health issues like untreated schizophrenia or bipolar disorder can affect driver eligibility under FMCSA standards.

Temporary disqualifications may occur until medical documentation or treatment proof is provided, as in manageably high blood pressure cases, whereas untreated seizures or defibrillator without exception may mean permanent disqualification.

References:
- FMCSA Medical Examiner Physical Qualification Standards (§391.41)
- 49 CFR Part 391 - Qualification of Drivers
- Medical Examiner's Handbook
- FMCSA Driver Exemptions

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Lake City DNA Testing

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Lake City Industry Training

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Lake City Hair Drug Testing

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Lake City Alcohol Testing

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Lake City Drug Testing Services

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Lake City 5 Panel Drug Test

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Lake City DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Lake City, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT physical typically includes a review of the driver’s health history and medications, vision screening, hearing assessment, vital signs (blood pressure, pulse), urinalysis (checking specific gravity, protein, glucose), and a full physical examination by a certified medical examiner. Upon passing, a Medical Examiner’s Certificate (DOT medical card) may be issued for up to 24 months.
You must have a valid DOT medical certificate if you: hold a commercial driver’s license (CDL) Class A, B or C for interstate commerce; operate a vehicle over 10,001 lbs GVWR in interstate commerce; transport more than eight passengers (for hire) or over fifteen passengers (not for hire); or transport hazardous materials requiring placarding under DOT regulations.
DOT physicals are offered at test centres in all cities and all states, through Accredited Drug Testing’s nationwide network. You can schedule by calling 1-800-221-4291.
You should bring a government-issued photo ID; glasses or contact lenses and the prescription; a list of medications and dosages; CPAP usage data if applicable; and any specialist clearance letters or physician documentation (for conditions like heart disease, sleep apnea, diabetes) to your DOT physical.
If you fail the DOT physical, you will not receive the medical card; you may be temporarily or permanently disqualified depending on the condition; you will need to address any medical issues (e.g., uncontrolled hypertension, untreated sleep apnea, recent myocardial infarction) and then be re-examined.

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(800) 221-4291