DOT Physical Exams - Lexington, IN

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With 36 testing centers in the Lexington area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

36 DOT Drug-Testing Centers in Lexington

Minutes Away, Test Today

705 Fairground Rd

8.09 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM (Lunch: 12:00 PM - 12:30 PM)⚡ Drug Screens: Mon–Fri: 06:30 AM - 02:30 PM
705 Fairground Rd
Scottsburg, IN 47170

40 E Cherry St

8.10 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM (Lunch: 12:00 PM - 12:30 PM)⚡ Drug Screens: Mon–Fri: 07:00 AM - 03:00 PM
40 E Cherry St
Scottsburg, IN 47170

1366 N. Gardner Street Hwy. 31 Ste. 129

9.29 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 03:30 PM
1366 N. Gardner Street Hwy. 31 Ste. 129
Salem, IN 47167

1451 N Gardner St

9.38 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 03:30 PM
1451 N Gardner St
Scottsburg, IN 47170

1801 Clifty Dr

13.46 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:30 PM✓ Walk-in with Electronic Donor Pass
1801 Clifty Dr
Madison, IN 47250

445 Clifty Dr

14.96 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM✓ Walk-in with Electronic Donor Pass
445 Clifty Dr
Madison, IN 47250

311 E Clifty Dr

15.73 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM (Lunch: 12:00 PM - 12:30 PM)✓ Walk-in with Electronic Donor Pass
311 E Clifty Dr
Madison, IN 47250

1373 E Sr 62

18.58 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM⚡ Drug Screens: Mon–Fri: 06:30 AM - 02:30 PM
1373 E Sr 62
Madison, IN 47250

130 Hunter Station Way Ste 101

20.25 mi
🕑 Facility: Mon–Fri: 06:30 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 03:00 PM
130 Hunter Station Way Ste 101
Sellersburg, IN 47172

1015 New Moody Ln

22.13 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1015 New Moody Ln
Lagrange, KY 40031

3118 E 10th St

23.07 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 11:30 AM, 12:30 PM - 02:30 PM
3118 E 10th St
Jeffersonville, IN 47130

3697 Charlestown Rd

23.26 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 02:00 PM - 04:00 PM
3697 Charlestown Rd
New Albany, IN 47150

2201 Greentree N

23.46 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:00 AM, 12:30 PM - 04:00 PM
2201 Greentree N
Clarksville, IN 47129

2051 Clevidence Blvd Ste A

23.55 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:00 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:30 AM, 01:00 PM - 03:30 PM
2051 Clevidence Blvd Ste A
Clarksville, IN 47129

800 Highlander Point Dr Ste 103

23.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
800 Highlander Point Dr Ste 103
Floyds Knobs, IN 47119

2145 N State Highway 3

24.23 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:30 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 07:00 AM - 03:30 PM
2145 N State Highway 3
North Vernon, IN 47265

1615 Blackiston View Dr

24.25 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 03:30 PM
1615 Blackiston View Dr
Clarksville, IN 47129

3605 Northgate Ct Ste 110

24.45 mi
🕑 Facility: Mon–Fri: 08:00 AM - 08:00 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 05:00 PM
3605 Northgate Ct Ste 110
New Albany, IN 47150

9880 Angies Way Ste 160

24.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9880 Angies Way Ste 160
Louisville, KY 40241

825 University Woods Dr Ste 12

24.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
825 University Woods Dr Ste 12
New Albany, IN 47150

2478 Us Highway 227 Ste 1

24.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2478 Us Highway 227 Ste 1
Carrollton, KY 41008

309 11th St

24.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
309 11th St
Carrollton, KY 41008

301 Henry St Bldg B

24.83 mi
🕑 Facility: Mon–Fri: 06:00 AM - 02:00 PM⚡ Drug Screens: Mon–Fri: 06:00 AM - 02:00 PM
301 Henry St Bldg B
North Vernon, IN 47265

1130 Medical Pl

24.93 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM✓ Walk-in with Electronic Donor Pass
1130 Medical Pl
Seymour, IN 47274

1124 Medical Pl

24.93 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM✓ Walk-in with Electronic Donor Pass
1124 Medical Pl
Seymour, IN 47274

3215 Westport Green Pl

25.18 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3215 Westport Green Pl
Louisville, KY 40241

1602 E 10th St

25.43 mi
🕑 Facility: Mon–Fri: 07:00 AM - 08:00 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 05:00 PM
1602 E 10th St
Jeffersonville, IN 47130

411 W Tipton St

25.45 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 02:30 PM
411 W Tipton St
Seymour, IN 47274

2232 Holiday Manor Ctr

25.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2232 Holiday Manor Ctr
Louisville, KY 40222

911 N Shelby St

26.18 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 02:00 PM - 04:00 PM
911 N Shelby St
Salem, IN 47167

7400 New Lagrange Rd Ste 303

26.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7400 New Lagrange Rd Ste 303
Louisville, KY 40222

1220 Missouri Ave

26.31 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 02:00 PM - 04:00 PM
1220 Missouri Ave
Jeffersonville, IN 47130

1321 Herr Ln Ste 195

26.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1321 Herr Ln Ste 195
Louisville, KY 40222

2400 Eastpoint Pkwy Ste 100

26.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2400 Eastpoint Pkwy Ste 100
Louisville, KY 40223

3920 Dutchmans Pkwy Emergency Dept

26.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3920 Dutchmans Pkwy Emergency Dept
Louisville, KY 40207

228 Breckenridge Ln 5

26.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
228 Breckenridge Ln 5
Louisville, KY 40207

Lexington, IN DOT Physical - What You Need to Know

A DOT physical is a thorough health evaluation mandated by the Department of Transportation (DOT) for all commercial motor vehicle (CMV) operators. This assessment ensures that drivers are in a condition suitable for handling large vehicles on public roadways. Conducted by a certified medical examiner (CME) listed on the National Registry of Certified Medical Examiners (NRCME), passing this exam grants drivers a DOT medical certificate (Medical Examiners Certificate or DOT medical card), essential for keeping a valid CDL per FMCSA rules. For assistance with DOT physical requirements, contact 800-221-4291.

During your exam, the medical examiner will complete a thorough health assessment to determine if you meet the Federal Motor Carrier Safety Administration (FMCSA) medical standards. The process typically includes:

What Does a DOT Physical Include?

Health History Review
Vision Test

Hearing Test
Urinalysis

Vital Signs
Physical Examination

DOT Testing | DOT Breath Alcohol | DOT Consortium Services | MVR Reports Also Available


What is checked in a DOT Physical?

  • Health history review and medication assessment
  • Vision screening (corrective lenses allowed; minimum standards apply)
  • Hearing assessment (forced whisper or audiometry)
  • Blood pressure and pulse rate
  • Physical examination (systems review)
  • Urinalysis (specific gravity, protein, glucose)

Upon successful completion, your Medical Examiner's Certificate (MEC) may be issued for up to 24 months. Some conditions may require shorter certification or specialist documentation

This applies specifically to Lexington, WI.

Who needs a DOT Physical?

You must possess a current DOT medical certificate if:

  • Holding a CDL - class A, B, C for interstate activities
  • Operating vehicles over 10,001 lbs. GVWR for interstate trade
  • Transporting more than 8 paid or over 15 unpaid passengers
  • Conveying hazardous materials needing placards
  • Working for DOT-regulated employers like FMCSA, FAA, FRA, FTA, or USCG that require medical qualification

Lexington, NE laws apply.

What to bring with you to a DOT Physical?

  • Bring an official photo ID
  • Remember your glasses/contacts and their prescriptions
  • Medication details with dosages listed
  • CPAP usage records (if applicable)
  • Specialist approvals (re: cardiology, sleep apnea, diabetes, etc.)
  • Current A1C/blood pressure records if tracked

Essential items to bring for your medical check-up in Lexington, MI include the above for ensuring a precise examination and necessary documentation to aid the certification process.

What happens if I fail a DOT Physical?

  • No Medical card will be issued
  • Temporary disqualification
  • Referral for treatment to your primary care doctor
  • FMCSA exemptions for certain medical conditions
  • Employer Notification
  • Next Steps (address the medical issues and retake the exam)

Location: Lexington, OH

What Will Fail a DOT Physical?

Medical Conditions That Can Fail a DOT Physical

  • Vision & Hearing: Vision below 20/40 in each eye (with correction), inability to differentiate signal colors, or hearing loss inhibiting recognition of a whispered message at 5 feet.
  • Blood Pressure & Heart Disease: Uncontrolled high blood pressure (≥180/110 mmHg), recent myocardial infarction, stroke, uncontrolled angina, or cardiac devices requiring special permission.
  • Diabetes: Poorly managed diabetes causing hypoglycemic episodes or complications (like neuropathy) impairing safe vehicle operation.
  • Sleep Apnea & Respiratory Issues: Untreated sleep apnea causing sleepiness, or serious lung conditions affecting oxygen levels.
  • Neurological Disorders: Conditions like epilepsy or seizure disorders (excluding exemptions) that may lead to loss of consciousness.
  • Substance Abuse: Active illegal drug use, alcohol addiction, or misuse of medications impacting driving.
  • Psychiatric & Cognitive Disorders: Severe mental health issues unmanaged by medication or cognitive defects impacting safety decisions.

Lexington Drug Testing

Quickly find trusted local drug testing centers in Lexington, IN — fast, convenient, and reliable every time!

Lexington DOT Drug Testing

Quickly find a local DOT drug testing center in Lexington, IN — fast, reliable, convenient nationwide service!

Lexington DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Lexington, IN.

Lexington Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Lexington, IN.

Lexington Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Lexington, IN locations—results you can trust, every time!

Lexington Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Lexington, IN.

Lexington Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Lexington, IN.

Lexington 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Lexington, IN employers and individuals nationwide.

Lexington 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Lexington, IN workplace and personal testing needs.

Lexington On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Lexington, IN workplace compliant.

Lexington DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Lexington, IN drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT physical typically includes a review of the driver’s health history and medications, vision screening, hearing assessment, vital signs (blood pressure, pulse), urinalysis (checking specific gravity, protein, glucose), and a full physical examination by a certified medical examiner. Upon passing, a Medical Examiner’s Certificate (DOT medical card) may be issued for up to 24 months.
You must have a valid DOT medical certificate if you: hold a commercial driver’s license (CDL) Class A, B or C for interstate commerce; operate a vehicle over 10,001 lbs GVWR in interstate commerce; transport more than eight passengers (for hire) or over fifteen passengers (not for hire); or transport hazardous materials requiring placarding under DOT regulations.
DOT physicals are offered at test centres in all cities and all states, through Accredited Drug Testing’s nationwide network. You can schedule by calling 1-800-221-4291.
You should bring a government-issued photo ID; glasses or contact lenses and the prescription; a list of medications and dosages; CPAP usage data if applicable; and any specialist clearance letters or physician documentation (for conditions like heart disease, sleep apnea, diabetes) to your DOT physical.
If you fail the DOT physical, you will not receive the medical card; you may be temporarily or permanently disqualified depending on the condition; you will need to address any medical issues (e.g., uncontrolled hypertension, untreated sleep apnea, recent myocardial infarction) and then be re-examined.

Customer Reviews

Explained everything so no questions

Torin was great. Explained everything so no questions

Tony Lombardo - 4/18/2025

Great experience

Great experience and great lab. Customer service agent was very helpful.

Jenna Bee - 2/19/2025

I love the customer service

I love the customer service. The lady was patient enough to help me book my appointment with ease and ensuring I get the location that's best for me. Keep up the good work!

Elizabeth Chiaka - 9/19/2024

Extremely pleasant

Initially Torrie helped me but I needed to call back. When I called back Kayla stepped in and finished my order. Both were extremely pleasant over the phone and provided wonderful customer service. Kudos to them!

Brynne Beverly - 4/19/2024

Super helpful

Customer service was surprisingly super helpful and so nice. She was able to get me in at a local location right away the same day. She helped me figure out exactly what I needed. Very impressed.

Brook - 9/19/2024

Fantastic Experience

Fantastic experience from the beginning. Jocelyn was very helpful to me as someone who doesn’t have much experience in needing a test. They were very helpful and walked me through the entire process start to finish. Thank you ADT!

Riley Wilson - 2/18/2025

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025

Kind and very helpful

Called around for hours and posted from company to person and got nowhere and had to fight to get a refund die to there miss information, well save time and sanity Acredited is the ONLY place the person was kind and very helpful, said what I needed and Faith the lady I dealt with made it simple and she did above and beyond everything I needed, as I'm going through a devorse this was a great help and made my day 100 stars from me.

Steven Harrison - 12/19/2024

Very easy to get scheduled

Very easy to get scheduled and easy process once checked in and the staff were all extremely friendly! I would recommend this company!

Ronickia Moore - 11/19/2024

Very Fun and Easy

Trish last week and Tatiana this week, very fun and easy folks to deal with. Well be using them more and more in the future.

Tom O - 12/19/2024


(800) 221-4291