DOT Physical Exams - Twilight, WV

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With 36 testing centers in the Twilight area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

(800) 221-4291

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36 DOT Drug-Testing Centers in Twilight

Minutes Away, Test Today

10 Elm Grove Crossing Mall

2.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10 Elm Grove Crossing Mall
Wheeling, WV 26003
Call (800) 221-4291 Get Donor Pass

1 Medical Park

5.82 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:00 PM (Lunch: 12:30 PM - 01:00 PM)✓ Walk-in with Electronic Donor Pass
1 Medical Park
Wheeling, WV 26003
Call (800) 221-4291 Get Donor Pass

24 Homestead Ave

6.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
24 Homestead Ave
Wheeling, WV 26003
Call (800) 221-4291 Get Donor Pass

620 National Rd Ste 300

6.47 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
620 National Rd Ste 300
Wheeling, WV 26003
Call (800) 221-4291 Get Donor Pass

2000 Eoff St

6.96 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2000 Eoff St
Wheeling, WV 26003
Call (800) 221-4291 Get Donor Pass

92 N 4th St Ste 3

8.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
92 N 4th St Ste 3
Martins Ferry, OH 43935
Call (800) 221-4291 Get Donor Pass

90 N 4th St

8.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
90 N 4th St
Martins Ferry, OH 43935
Call (800) 221-4291 Get Donor Pass

3948 Central Ave

9.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3948 Central Ave
Shadyside, OH 43947
Call (800) 221-4291 Get Donor Pass

800 Wheeling Ave Ste 1w

10.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
800 Wheeling Ave Ste 1w
Glen Dale, WV 26038
Call (800) 221-4291 Get Donor Pass

1585 Wheeling Avenue

10.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1585 Wheeling Avenue
Glendale, WV 26038
Call (800) 221-4291 Get Donor Pass

1307 Lafayette Ave

11.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1307 Lafayette Ave
Moundsville, WV 26041
Call (800) 221-4291 Get Donor Pass

51520 National Rd E

14.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
51520 National Rd E
Saint Clairsville, OH 43950
Call (800) 221-4291 Get Donor Pass

51339 National Rd E

14.46 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
51339 National Rd E
Saint Clairsville, OH 43950
Call (800) 221-4291 Get Donor Pass

National Road East

15.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
National Road East
Saint Clairsville, OH 43950
Call (800) 221-4291 Get Donor Pass

350 Highway 7 N

15.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
350 Highway 7 N
Powhatan Point, OH 43942
Call (800) 221-4291 Get Donor Pass

95 Leonard Ave Ste 401, Bldg1

19.71 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-12:30 PM & 2:00 PM-3:00 PM • Sat: 8:30 AM-11:30 AM✓ Walk-in with Electronic Donor Pass
95 Leonard Ave Ste 401, Bldg1
Washington, PA 15301
Call (800) 221-4291 Get Donor Pass

140 Church St Ste 102

20.24 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM (Lunch: 12:30 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 07:00 AM - 12:00 PM, 01:00 PM - 03:00 PM
140 Church St Ste 102
Rogersville, PA 15359
Call (800) 221-4291 Get Donor Pass

181 W Beau St

20.36 mi
🕑 Testing Hours: Mon–Fri: 2:00 PM-4:00 PM✓ Walk-in with Electronic Donor Pass
181 W Beau St
Washington, PA 15301
Call (800) 221-4291 Get Donor Pass

1417 Main St

20.65 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1417 Main St
Follansbee, WV 26037
Call (800) 221-4291 Get Donor Pass

90 West Chestnut Street, Millcraft Center

20.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
90 West Chestnut Street, Millcraft Center
Washington, PA 15301
Call (800) 221-4291 Get Donor Pass

155 Wilson Ave

20.90 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-11:30 AM & 1:00 PM-3:30 PM✓ Walk-in with Electronic Donor Pass
155 Wilson Ave
Washington, PA 15301
Call (800) 221-4291 Get Donor Pass

460 Washington Rd Ste 7

22.82 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
460 Washington Rd Ste 7
Washington, PA 15301
Call (800) 221-4291 Get Donor Pass

501 W High St

23.13 mi
🕑 Facility: Mon–Fri: 08:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 02:00 PM - 04:00 PM
501 W High St
Waynesburg, PA 15370
Call (800) 221-4291 Get Donor Pass

3203 Johnson Rd

23.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3203 Johnson Rd
Steubenville, OH 43952
Call (800) 221-4291 Get Donor Pass

539 Ward Dr

23.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
539 Ward Dr
Wintersville, OH 43953
Call (800) 221-4291 Get Donor Pass

4220 Sunset Blvd

23.88 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4220 Sunset Blvd
Steubenville, OH 43952
Call (800) 221-4291 Get Donor Pass

35 N Porter St

23.94 mi
🕑 Facility: Mon–Fri: 08:00 AM - 03:30 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 02:30 PM
35 N Porter St
Waynesburg, PA 15370
Call (800) 221-4291 Get Donor Pass

350 Bonar Ave

24.11 mi
🕑 Facility: Mon–Fri: 06:30 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 07:30 AM - 11:00 AM, 01:00 PM - 02:00 PM
350 Bonar Ave
Waynesburg, PA 15370
Call (800) 221-4291 Get Donor Pass

500 Luray Dr

24.82 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
500 Luray Dr
Wintersville, OH 43953
Call (800) 221-4291 Get Donor Pass

601 Colliers Way

24.83 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
601 Colliers Way
Weirton, WV 26062
Call (800) 221-4291 Get Donor Pass

218 Three Springs Dr

25.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
218 Three Springs Dr
Weirton, WV 26062
Call (800) 221-4291 Get Donor Pass

951 E Market St

25.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
951 E Market St
Cadiz, OH 43907
Call (800) 221-4291 Get Donor Pass

100 Medical Blvd

25.88 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
100 Medical Blvd
Canonsburg, PA 15317
Call (800) 221-4291 Get Donor Pass

2629 Mount Morris Rd

26.13 mi
🕑 Facility: Mon–Fri: 08:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 10:00 AM - 11:30 AM, 02:00 PM - 03:30 PM
2629 Mount Morris Rd
Waynesburg, PA 15370
Call (800) 221-4291 Get Donor Pass

113 Cavasina Dr Ste 7

27.07 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-12:00 PM & 1:00 PM-3:00 PM✓ Walk-in with Electronic Donor Pass
113 Cavasina Dr Ste 7
Canonsburg, PA 15317
Call (800) 221-4291 Get Donor Pass

135 Loves Hill Ext

27.87 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
135 Loves Hill Ext
Waynesburg, PA 15370
Call (800) 221-4291 Get Donor Pass

Twilight, WV DOT Physical - What You Need to Know

DOT Physical: A thorough health check mandated by the Department of Transportation (DOT) for commercial vehicle operators (CMV) ensures drivers are apt physically, mentally, and emotionally for operating massive vehicles safely on public routes. Conducted by a certified medical examiner (CME) on the National Registry of Certified Medical Examiners (NRCME), passing grants a DOT medical certificate, vital for holding a valid CDL per FMCSA rules. Need help determining which exam applies? Call 800-221-4291.

During your exam, the medical examiner will complete a thorough health assessment to determine if you meet the Federal Motor Carrier Safety Administration (FMCSA) medical standards. The process typically includes:

What Does a DOT Physical Include?

Health History Review
Vision Test

Hearing Test
Urinalysis

Vital Signs
Physical Examination

DOT Testing | DOT Breath Alcohol | DOT Consortium Services | MVR Reports Also Available


What is checked in a DOT Physical?

  • Review of health history and medications
  • Vision check (corrective lenses allowed; must meet minimum standards)
  • Hearing evaluation (such as forced whisper test)
  • Blood pressure and pulse measurement
  • Physical exam (including systems review)
  • Urinalysis (for specific gravity, proteins, glucose)

Upon successful completion, your Medical Examiner's Certificate (MEC) may be issued for up to 24 months. Some conditions may require shorter certification or specialist documentation.

Location: Twilight, OH

Who needs a DOT Physical?

A valid DOT medical card is required in Twilight, MD for:

  • Holders of a CDL (class A, B, or C) crossing state lines
  • Drivers of vehicles exceeding 10,001 lbs GVWR in interstate business
  • Operators transporting over 8 hireable passengers or more than 15 without hire
  • Hauling hazardous materials needing placard under DOT regulations
  • Employees of DOT agency-regulated businesses, like FMCSA, FAA, FRA, FTA, or USCG needing medical eligibility

DOT Physical Near Twilight, WV

Nearby Testing Centers in West Virginia

Charleston, WV

Huntington, WV

Morgantown, WV

Parkersburg, WV

Wheeling, WV

Weirton, WV

Fairmont, WV

Middletown District, WV

Martinsburg, WV

Beckley, WV

What to bring with you to a DOT Physical?

  • Government-issued ID
  • Eyewear and its prescription
  • Medication list with doses
  • CPAP machine data, if used
  • Specialist approvals (cardiac issues, sleep apnea, diabetes, etc.)
  • Recent A1C/blood pressure logs, if monitored

Ensure these items are prepared before your Twilight, MD DOT examination to prevent potential delays or issues in the process.

What happens if I fail a DOT Physical?

  • No Medical card issued
  • Temporary disqualification
  • Referral for treatment to your primary care doctor
  • FMCSA exemptions for certain medical conditions
  • Employer Notification
  • Next Steps (address medical issues & retake the exam)

These procedures apply for medical exams in Twilight, IA.

What Will Fail a DOT Physical?

Medical Conditions That Can Fail a DOT Physical

  • Vision & Hearing: Less than 20/40 vision in both eyes, inability to distinguish traffic light colors, or inability to hear a forced whisper from 5 feet away.
  • Blood Pressure & Heart Disease: Hypertension uncontrolled (≥180/110 mmHg), recent heart attack, uncontrolled angina, stroke, or an uncleared defibrillator.
  • Diabetes: Uncontrolled diabetes resulting in low blood sugar episodes frequently or complications like neuropathy or impaired vision that hinder safe driving.
  • Sleep Apnea & Respiratory Conditions: Untreated sleep apnea causing drowsiness during the day or severe lung conditions obstructing oxygenation.
  • Neurological Conditions: Epilepsy or seizure disorders (unless exempt), or conditions leading to unconsciousness, dizziness, or uncontrolled shaking.
  • Substance Misuse: Current illicit drug use, alcohol dependency, or abuse of prescription drugs impacting driving.
  • Mental & Cognitive Disorders: Serious uncontrolled psychiatric conditions or cognitive impairments affecting judgment or reaction time.

Some matters might cause a temporary disqualification until medical documentation is supplied (controlled blood pressure, diabetes management). Others, like untreated seizures or defibrillators, are seen as permanently disqualifying by FMCSA.

References:
- FMCSA Medical Examiner Physical Qualification Standards (§391.41)
- 49 CFR Part 391 - Qualification of Drivers
- Medical Examiner's Handbook
- FMCSA Driver Exemptions

Twilight Drug Testing

Quickly find trusted local drug testing centers in Twilight, WV — fast, convenient, and reliable every time!

Twilight DOT Drug Testing

Quickly find a local DOT drug testing center in Twilight, WV — fast, reliable, convenient nationwide service!

Twilight DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Twilight, WV.

Twilight Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Twilight, WV.v

Twilight Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Twilight, WV locations—results you can trust, every time!

Twilight Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Twilight, WV.

Twilight Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Twilight, WV.

Twilight 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Twilight, WV employers and individuals nationwide.

Twilight 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Twilight, WV workplace and personal testing needs.

Twilight On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Twilight, WV workplace compliant.

Twilight DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Twilight, WV drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT physical typically includes a review of the driver’s health history and medications, vision screening, hearing assessment, vital signs (blood pressure, pulse), urinalysis (checking specific gravity, protein, glucose), and a full physical examination by a certified medical examiner. Upon passing, a Medical Examiner’s Certificate (DOT medical card) may be issued for up to 24 months.
You must have a valid DOT medical certificate if you: hold a commercial driver’s license (CDL) Class A, B or C for interstate commerce; operate a vehicle over 10,001 lbs GVWR in interstate commerce; transport more than eight passengers (for hire) or over fifteen passengers (not for hire); or transport hazardous materials requiring placarding under DOT regulations.
DOT physicals are offered at test centres in all cities and all states, through Accredited Drug Testing’s nationwide network. You can schedule by calling 1-800-221-4291.
You should bring a government-issued photo ID; glasses or contact lenses and the prescription; a list of medications and dosages; CPAP usage data if applicable; and any specialist clearance letters or physician documentation (for conditions like heart disease, sleep apnea, diabetes) to your DOT physical.
If you fail the DOT physical, you will not receive the medical card; you may be temporarily or permanently disqualified depending on the condition; you will need to address any medical issues (e.g., uncontrolled hypertension, untreated sleep apnea, recent myocardial infarction) and then be re-examined.

Customer Reviews

Really Helpful

I am just really happy to see that the location near me still offers hair follicle tests!! They are really helpful when any false positives happen, waiting on my results. Hope they come fast!

Kali Collins - 1/16/2025

Friendly and Knowledgeable

I reached out Accredited Drug Testing/Secure Screenings to partner for my drug testing business (Discovery Diagnostics Columbia SC) and the process was extremely easy. I worked with Sicilia who was amazing. She was friendly & knowledgeable, and answer all of my questions with ease.

Kendra Harley - 3/26/2025

Sweet and helpful

Trish was amazing and got me through the sytem very fast and swift. I had a hard time hearing her a couple of times, but she was super sweet and helpful throughout the process. Highly recommend her!

Sophia Schutze - 6/19/2024

Quick, Polite, Informative

Everyone has been quick to respond, polite, informative. It's been easy to order tests. I would recommend them to anyone to use.

April Rogers - 2/15/2025

Thank You!!

I needed a quick drug test for visitation with my children. I called at 6am they answered and set me up with a place a couple miles from my house to go get the test done, that was on Wednesday, I had the results on Friday afternoon and was able to see my kids on Saturday. Thank you for the help!

Mark Broadhead - 2/12/2025

Fast,easy and efficient

Fast,easy and efficient. They made the whole process stress free and simple. Thanks 😊

Micheal Ankenbrandt - 2/19/2025

Excellent service!

Excellent service! Trish went above and beyond to find me a testing site as close to my home as possible. Results were faster than expected, as well. Thanks for your help, Trish and Accredited!

Es Hache - 4/13/2025

Clear and Competent

I was so surprised with the quality of customer service I received today when I called Accredited Drug Testing. The representative was so clear and competent. She was able to reschedule an appointment at my preferred location. The representative whose name is Justice, was so polite and competent. She expedited the whole process. It was a relief to me.

Rachel Padian - 9/6/2024

phone support has been excellent

I have used ADT several times. Their phone support has been excellent, both when putting in the orders and when asking for help to interpret results.

Tee Ess - 12/19/2024

Very easy to get scheduled

Very easy to get scheduled and easy process once checked in and the staff were all extremely friendly! I would recommend this company!

Ronickia Moore - 11/19/2024


(800) 221-4291