DOT Physical Exams - Ontario, WI

Locations, All Cities, All States

FMCSA | FAA | USCG | FTA | FRA | PHMSA

With 33 testing centers in the Ontario area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

33 DOT Drug-Testing Centers in Ontario

Minutes Away, Test Today

500 Water Ave

13.52 mi
🕑 Facility: Mon–Fri: 08:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 11:30 AM, 01:30 PM - 04:30 PM
500 Water Ave
Hillsboro, WI 54634

325 Butts Ave

18.26 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 01:00 PM - 03:00 PM
325 Butts Ave
Tomah, WI 54660

321 Butts Ave

18.27 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM
321 Butts Ave
Tomah, WI 54660

110 W Wisconsin St

18.36 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 10:00 AM - 03:30 PM
110 W Wisconsin St
Sparta, WI 54656

315 W Oak St

18.67 mi
🕑 Facility: Mon–Fri: 08:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 01:00 PM - 04:00 PM
315 W Oak St
Sparta, WI 54656

310 W Main St

18.74 mi
🕑 Facility: Mon–Fri: 08:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 01:00 PM - 04:00 PM
310 W Main St
Sparta, WI 54656

407 S Main St

19.07 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-5:00 PM • Sat: 9:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
407 S Main St
Viroqua, WI 54665

407 S Main St Ste 400

19.07 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM • Sat: 9:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
407 S Main St Ste 400
Viroqua, WI 54665

507 S Main St Ste Rt

19.11 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
507 S Main St Ste Rt
Viroqua, WI 54665

1330 N Superior Ave

19.88 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 03:00 PM
1330 N Superior Ave
Tomah, WI 54660

1050 Division St

26.00 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
1050 Division St
Mauston, WI 53948

220 E State St Rm 104

26.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
220 E State St Rm 104
Mauston, WI 53948

1415 Us Hwy 16

27.95 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-3:30 PM✓ Walk-in with Electronic Donor Pass
1415 Us Hwy 16
La Crosse, WI 54601

301 E 2nd St

28.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
301 E 2nd St
Richland Center, WI 53581

2045 32nd St S

31.15 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM • Sat: 8:00 AM-3:00 PM • Sun: 1:00 PM-3:00 PM✓ Walk-in with Electronic Donor Pass
2045 32nd St S
La Crosse, WI 54601

3111 Gundersen Dr

31.33 mi
🕑 Facility: Mon–Fri: 08:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 08:45 AM - 11:45 AM, 01:45 PM - 04:15 PM
3111 Gundersen Dr
Onalaska, WI 54650

223 E Main St

32.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
223 E Main St
Reedsburg, WI 53959

2000 N Dewey Ave

32.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2000 N Dewey Ave
Reedsburg, WI 53959

1900 N Dewey Ave

32.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1900 N Dewey Ave
Reedsburg, WI 53959

1408 Wheelihan Ave

32.38 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 12:30 PM, 01:30 PM - 04:30 PM
1408 Wheelihan Ave
Necedah, WI 54646

1626 Oak St

32.89 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat: 8:00 AM-6:00 PM • Sun: 1:00 PM-6:00 PM✓ Walk-in with Electronic Donor Pass
1626 Oak St
La Crosse, WI 54603

630 10th St S

32.95 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-12:00 PM & 1:00 PM-4:30 PM✓ Walk-in with Electronic Donor Pass
630 10th St S
La Crosse, WI 54601

1910 South Ave

33.03 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1910 South Ave
La Crosse, WI 54601

1900 South Ave 3rd Fl

33.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1900 South Ave 3rd Fl
La Crosse, WI 54601

1303 Main St S

33.09 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 12:00 PM, 02:00 PM - 04:00 PM
1303 Main St S
Holmen, WI 54636

419 Sand Lake Rd

33.28 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:00 PM✓ Walk-in with Electronic Donor Pass
419 Sand Lake Rd
Onalaska, WI 54650

1526 Rose St Ste 100

33.77 mi
🕑 Testing Hours: M-Th 7:30 AM-5:00 PM • F 7:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
1526 Rose St Ste 100
La Crosse, WI 54603

226 N 2nd St

36.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
226 N 2nd St
La Crescent, MN 55947

1075 N Wisconsin Ave

37.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1075 N Wisconsin Ave
Muscoda, WI 53573

260 26th St

39.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
260 26th St
Prairie Du Sac, WI 53578

205 Park St

40.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
205 Park St
Boscobel, WI 53805

530 Wisconsin Dells Pkwy S

41.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
530 Wisconsin Dells Pkwy S
Wisconsin Dells, WI 53965

3 E Main St

41.26 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:30 AM, 01:30 PM - 03:00 PM
3 E Main St
Black River Falls, WI 54615

Ontario, WI DOT Physical - What You Need to Know

The Department of Transportation mandates a thorough health check-up for all commercial motor vehicle operators. The DOT physical is a key assessment to confirm that these drivers are safely equipped, both physically and mentally, to handle large vehicles on busy streets. Conducted by a certified medical professional listed in the National Registry, passing this test enables the issuance of a DOT medical certificate, necessary for maintaining a CDL license under FMCSA norms. If other DOT agencies regulate you, call 800-221-4291 for guidance.

During your exam, the medical examiner will complete a thorough health assessment to determine if you meet the Federal Motor Carrier Safety Administration (FMCSA) medical standards. The process typically includes:

What Does a DOT Physical Include?

Health History Review
Vision Test

Hearing Test
Urinalysis

Vital Signs
Physical Examination

DOT Testing | DOT Breath Alcohol | DOT Consortium Services | MVR Reports Also Available


What is checked in a DOT Physical?

  • Health history review and medication assessment
  • Vision screening (corrective lenses allowed; minimum standards apply)
  • Hearing assessment (forced whisper or audiometry)
  • Blood pressure and pulse rate
  • Physical examination (systems review)
  • Urinalysis (specific gravity, protein, glucose)

Upon successful completion, your Medical Examiner's Certificate (MEC) may be issued for up to 24 months. Some conditions may require shorter certification or specialist documentation in Ontario, KS.

Who needs a DOT Physical?

You must prove an active DOT medical certificate (card) in cases like these:

  • Ownership of a commercial driver's license (CDL)- class A, B, or C for crossing state lines
  • Piloting any vehicle beyond 10,001 pounds GVWR during interstate duties
  • Conveying more than 8 passengers (paid) or upward of 15 (non-paid)
  • Transporting hazardous substances needing DOT sign off
  • Employment under DOT agency rules like FMCSA, FAA, FRA, FTA, or USCG in Ontario, NH requesting DOT health competency

What to bring with you to a DOT Physical?

  • Government-issued photo 
  • Glasses/contacts & prescription
  • List of medications & dosages
  • CPAP usage data (if applicable)
  • Specialist clearances (cardiology, sleep apnea, diabetes, etc.)
  • Recent A1C/blood pressure logs if monitored

Ensure all required documents are up-to-date, especially when operating outside your home state, like Ontario, HI.

What happens if I fail a DOT Physical?

  • Issuance of Medical card denied
  • Imposed temporary disqualification
  • Directed to primary care doctor for treatment
  • Seek FMCSA exemptions for specific health issues
  • Notification to employer required
  • Plan next actions (tackle medical concerns and retake the test)

Ensure compliance with Ontario, MO regulations and guidelines, and prepare for potential reevaluation as necessitated.

What Will Fail a DOT Physical?

Medical Conditions That Can Fail a DOT Physical

  • Vision & Hearing: Vision below 20/40 in each eye (even with correction), inability to recognize traffic colors, or hearing loss that makes it hard to discern a whispered sound at five feet.
  • Blood Pressure & Heart Disease: Unmanaged high blood pressure (≥180/110 mmHg), recent heart events, unregulated angina, or a non-cleared implanted defibrillator.
  • Diabetes: Unregulated diabetes causing frequent low sugar levels or complications (e.g., neuropathy) impacting safe driving.
  • Sleep Apnea & Respiratory Issues: Unmanaged sleep apnea causing drowsiness, or severe respiratory diseases hindering proper oxygenation.
  • Neurological Disorders: Epileptic conditions (unless exempted) or illnesses causing loss of consciousness, dizziness, or intense tremors.
  • Substance Abuse: Current use of illicit drugs, habitual alcohol misuse, or prescription medication misuse impairing one's driving capabilities.
  • Psychiatric & Cognitive Disorders: Significant unmanaged psychiatric conditions (e.g., schizophrenia) or cognitive dysfunction affecting decision-making or reaction speeds.

Some circumstances may temporarily disqualify you until you show the required medical documentation (e.g., managed diabetes). Issues like untreated seizure disorders or non-cleared defibrillators are typically seen as permanently disqualifying by FMCSA norms.

Ontario Drug Testing

Quickly find trusted local drug testing centers in Ontario, WI — fast, convenient, and reliable every time!

Ontario DOT Drug Testing

Quickly find a local DOT drug testing center in Ontario, WI — fast, reliable, convenient nationwide service!

Ontario DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Ontario, WI.

Ontario Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Ontario, WI.v

Ontario Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Ontario, WI locations—results you can trust, every time!

Ontario Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Ontario, WI.

Ontario Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Ontario, WI.

Ontario 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Ontario, WI employers and individuals nationwide.

Ontario 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Ontario, WI workplace and personal testing needs.

Ontario On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Ontario, WI workplace compliant.

Ontario DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Ontario, WI drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT physical typically includes a review of the driver’s health history and medications, vision screening, hearing assessment, vital signs (blood pressure, pulse), urinalysis (checking specific gravity, protein, glucose), and a full physical examination by a certified medical examiner. Upon passing, a Medical Examiner’s Certificate (DOT medical card) may be issued for up to 24 months.
You must have a valid DOT medical certificate if you: hold a commercial driver’s license (CDL) Class A, B or C for interstate commerce; operate a vehicle over 10,001 lbs GVWR in interstate commerce; transport more than eight passengers (for hire) or over fifteen passengers (not for hire); or transport hazardous materials requiring placarding under DOT regulations.
DOT physicals are offered at test centres in all cities and all states, through Accredited Drug Testing’s nationwide network. You can schedule by calling 1-800-221-4291.
You should bring a government-issued photo ID; glasses or contact lenses and the prescription; a list of medications and dosages; CPAP usage data if applicable; and any specialist clearance letters or physician documentation (for conditions like heart disease, sleep apnea, diabetes) to your DOT physical.
If you fail the DOT physical, you will not receive the medical card; you may be temporarily or permanently disqualified depending on the condition; you will need to address any medical issues (e.g., uncontrolled hypertension, untreated sleep apnea, recent myocardial infarction) and then be re-examined.

Customer Reviews

test was quick and easy

Company New Hire Employee Process for DOT Drug test was quick and easy. No need to make appt. All employee had to do is show prepaid qr code I sent to employee show ID and was set. Would Recommend.

Maria Lopez - 11/19/2024

I love the customer service

I love the customer service. The lady was patient enough to help me book my appointment with ease and ensuring I get the location that's best for me. Keep up the good work!

Elizabeth Chiaka - 9/19/2024

Great experience

Great experience and great lab. Customer service agent was very helpful.

Jenna Bee - 2/19/2025

Pretty Straightforward

It was a pretty straightforward process getting an appointment and making payment. Getting to the testing site was quick. I was in and out in no time.

Sadiat Ogunyemi - 12/19/2024

Friendly and Knowledgeable

I reached out Accredited Drug Testing/Secure Screenings to partner for my drug testing business (Discovery Diagnostics Columbia SC) and the process was extremely easy. I worked with Sicilia who was amazing. She was friendly & knowledgeable, and answer all of my questions with ease.

Kendra Harley - 3/26/2025

Thank You!!

I needed a quick drug test for visitation with my children. I called at 6am they answered and set me up with a place a couple miles from my house to go get the test done, that was on Wednesday, I had the results on Friday afternoon and was able to see my kids on Saturday. Thank you for the help!

Mark Broadhead - 2/12/2025

Saved My CDL!

Time was running out before my Cdl got downgraded because of a violation I had on clearinghouse. I couldn't find an employer to send me for my return to duty test, but these guys had my test scheduled and done in the same day! They saved my cdl. Thank you again!

Michael Williams - 12/2/2024

This is by far the easiest

This is by far the easiest way to get my lab work ordered and paid for. The phone calls are short and to the point. They don’t try to push extra sales on you and when I walk in to the clinic I simply show my donor pass and with in a matter of minutes I’m done. I will continue to use ADT in the future.

Jason Jackson - 7/19/2025

Fast and easy

It was fast and easy a pleasure to talk to the customer service people great service

Peter Villegas - 1/19/2025

Extremely polite

They were very fast extremely polite walked you through every detail of the process if you had any questions they had no problems answering

Billy Williams - 2/19/2025


(800) 221-4291