Urine| Hair Follicle | Oral Fluid | Blood | ETG
Hair testing is recognized as an effective measure for identifying drug and alcohol consumption. Hair preserves a comprehensive history of substance intake by embedding biomarkers within the fabric of the elongating hair strand. When sampled near the scalp, hair can reveal a detection period of up to around three months for alcohol and other substances. Hair collection is straightforward, relatively challenging to tamper with, and convenient to transport.
A 1.5-inch section of approximately 200 hair strands (roughly the thickness of a #2 pencil) is needed near the scalp to obtain 100mg, which is the ideal quantity for testing and verification. For EtG, additional tests, and/or analyses beyond 10 panels, a 150mg sample amount is advisable. We suggest using a jeweler's scale for precise weighing of the specimen. Should scalp hair be absent, an equivalent quantity of body hair may be utilized. Here, head hair pertains solely to scalp hair. Body hair encompasses all other hair categories (facial, axillary, etc.).
Process Overview
The core process stages in the lab analysis of a drug test include Accessioning, Screening, Extraction, and Confirmation.
Accessioning pertains to the initial incorporation of a sample into a laboratory's infrastructure. This step involves confirmation of proper sample sealing and dispatch, allocation of a random LAN (Laboratory Accessioning Number), and completion of any outstanding data entry not captured by an electronic chain of custody framework.
Screening entails a preliminary rapid examination for drugs of abuse. While Screening is a cost-efficient method to exclude drug use for most samples, a positive outcome needs confirmation to hold in court. Samples that are presumptively positive in Screening require a secondary confirmation.
If a sample shows a presumptive positive result during Screening, additional hair is collected from the initial sample for the Extraction phase. During Extraction, drugs are isolated from hair at a significantly lower concentration than other methods (e.g., urine or oral fluid), making hair drug screening the most complex method to execute.
Confirmation of any positive Screen outcome is performed using GC/MS, GC/MS/MS, or LC/MS/MS. All presumptive positive samples are cleansed before confirmation if needed. The comprehensive laboratory workflow from Accessioning to Confirmation is evaluated under both the CAP (College of American Pathologists) Hair classification and ISO / IEC 17025 accreditation standards.
Advantages of hair drug testing:
Limitations:
Note: Though referred to as "hair follicle tests," the procedure scrutinizes the hair shaft and not the hair follicle beneath the scalp.
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