Hair Follicle Drug Testing - Springfield, KY

Employment, DOT, Court Ordered, Personal
Urine| Hair Follicle | Oral Fluid | Blood | ETG

With 38 testing centers in the Springfield area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

38 Hair Drug Testing Centers in Springfield

Minutes Away, Test Today

420 Loretto Rd Ste 300

8.18 mi
🕑 Testing Hours: M-Sun: 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
420 Loretto Rd Ste 300
Lebanon, KY 40033

320 Loretto Rd

8.20 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
320 Loretto Rd
Lebanon, KY 40033

727 W Main St

8.93 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:30 PM✓ Walk-in with Electronic Donor Pass
727 W Main St
Lebanon, KY 40033

700 Portland Ave Ste B

16.62 mi
🕑 Testing Hours: M-Sun: 24 hrs✓ Walk-in with Electronic Donor Pass
700 Portland Ave Ste B
Bardstown, KY 40004

700 Portland Ave

16.62 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-7:00 PM✓ Walk-in with Electronic Donor Pass
700 Portland Ave
Bardstown, KY 40004

919 E. Chambers Blvd

16.84 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:00 AM, 01:00 PM - 03:00 PM
919 E. Chambers Blvd
Bardstown, KY 40004

809 Morton Ave Ste 200

16.90 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-2:30 PM✓ Walk-in with Electronic Donor Pass
809 Morton Ave Ste 200
Bardstown, KY 40004

118 Patriot Dr Ste 102

20.17 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
118 Patriot Dr Ste 102
Bardstown, KY 40004

470 Linden Ave Ste 3

21.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
470 Linden Ave Ste 3
Harrodsburg, KY 40330

464 Linden Ave

21.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
464 Linden Ave
Harrodsburg, KY 40330

1509 Louisville Rd

21.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1509 Louisville Rd
Harrodsburg, KY 40330

150 W Bear Track Rd

22.93 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
150 W Bear Track Rd
Campbellsville, KY 42718

1700 Old Lebanon Rd

23.00 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
1700 Old Lebanon Rd
Campbellsville, KY 42718

91 Kingswood Dr

23.06 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
91 Kingswood Dr
Campbellsville, KY 42718

380 Whirlaway Dr

23.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
380 Whirlaway Dr
Danville, KY 40422

211 S 3rd St

24.76 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
211 S 3rd St
Danville, KY 40422

104 Nancy Cox Dr Ste A

24.92 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-2:30 PM✓ Walk-in with Electronic Donor Pass
104 Nancy Cox Dr Ste A
Campbellsville, KY 42718

100 Walton Ave

25.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
100 Walton Ave
Danville, KY 40422

101 Daniel Dr

25.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
101 Daniel Dr
Danville, KY 40422

187 Wolford Ave

29.97 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM (Lunch: 12:00 PM - 12:30 PM)✓ Walk-in with Electronic Donor Pass
187 Wolford Ave
Liberty, KY 42539

1903 W Hebron Ln Emergency Dept

31.59 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 9:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
1903 W Hebron Ln Emergency Dept
Shepherdsville, KY 40165

181 Highway 44 E Ste 1

32.16 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 9:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
181 Highway 44 E Ste 1
Shepherdsville, KY 40165

110 Metker Trl

32.54 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
110 Metker Trl
Stanford, KY 40484

202-206 Milby St

34.12 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
202-206 Milby St
Greensburg, KY 42743

189 Adam Shepherd Pkwy Ste 13

34.26 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 9:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
189 Adam Shepherd Pkwy Ste 13
Shepherdsvlle, KY 40165

438 Adam Shepherd Pkwy Ste 2

34.26 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 9:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
438 Adam Shepherd Pkwy Ste 2
Shepherdsville, KY 40165

2412 Ring Rd Ste 100

34.36 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 9:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
2412 Ring Rd Ste 100
Elizabethtown, KY 42701

2412 Ring Rd Ste 200

34.36 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 9:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
2412 Ring Rd Ste 200
Elizabethtown, KY 42701

3925 Frankfort Ford Rd

34.45 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3925 Frankfort Ford Rd
Wilmore, KY 40390

110 Pleasant Retreat Dr

35.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
110 Pleasant Retreat Dr
Lancaster, KY 40444

134 Heartland Dr

35.76 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
134 Heartland Dr
Elizabethtown, KY 42701

620 Westport Rd Ste A

36.07 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat:,Sun: 9:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
620 Westport Rd Ste A
Elizabethtown, KY 42701

727 Hospital Dr

36.28 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-12:30 PM & 1:00 PM-2:00 PM✓ Walk-in with Electronic Donor Pass
727 Hospital Dr
Shelbyville, KY 40065

1111 Ring Rd

36.28 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-3:00 PM • Sat: 9:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
1111 Ring Rd
Elizabethtown, KY 42701

101 Stonecrest Rd Ste 101

36.46 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
101 Stonecrest Rd Ste 101
Shelbyville, KY 40065

117 Quinn Dr

36.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
117 Quinn Dr
Nicholasville, KY 40356

60 Mack Walters Rd Rear Entrance

36.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
60 Mack Walters Rd Rear Entrance
Shelbyville, KY 40065

101 Medical Heights Dr Ste G

37.04 mi
🕑 Facility: Mon–Fri: 06:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 01:00 PM - 03:30 PM
101 Medical Heights Dr Ste G
Frankfort, KY 40601

Employment, Court Ordered, Probation, Family

Accredited Drug Testing offers comprehensive hair follicle drug analysis right in Springfield, Kentucky. If you're in need of either a hair drug or alcohol assessment, Accredited Drug Testing is your go-to expert with centers available across the nation, enabling hair sample collection for testing. Most scenarios require 1.5 to 2 inches of hair, whether from the scalp or body. This evaluation method checks for drugs, alcohol/ETG, and can be customized for other testing frameworks.

Recently, hair follicle testing has been gaining favor as the preferred drug testing method. Employers, legal authorities, and substance abuse professionals often mandate hair assessments over traditional urine tests. These tests are essential for employers with strict no-drug policies, court mandates, and probation checks. The major advantage lies in the extended detection timeline of up to 90 days, secured by supervised sample collection, making it nearly immune to test manipulation. However, for detecting recent drug use within a five-day period, urine tests remain unparalleled.

What it is, How it works

Hair analysis is increasingly recognized as an effective method for identifying drug and alcohol consumption. By encapsulating biomarkers within the fibers of a hair strand as it grows, hair offers a prolonged history of substance use. When collected close to the scalp, it can indicate the presence of alcohol and drugs up to about a 3-month period. Hair collection is straightforward, relatively hard to tamper with, and simple to transport.

A hair sample measuring 1.5 inches and consisting of around 200 strands (approximately the thickness of a #2 pencil) near the scalp provides 100mg of hair, which is the perfect amount for both screening and confirmation. For EtG, supplementary tests, and/or more than 10-panel tests, 150mg of the sample is advised. It is best to weigh the sample using a jeweler’s scale. If scalp hair is not available, a similar amount of body hair can be used. When we talk about head hair, we mean only scalp hair. Body hair refers to all other types, such as facial or axillary hair.

Process Overview

The laboratory process for evaluating a drug test outcome comprises four critical steps: Accessioning, Screening, Extraction, and Confirmation.

During Accessioning, a sample is initially processed into the laboratory’s system. This step ensures the sample was securely sealed and dispatched correctly, assigns a random LAN (Laboratory Accessioning Number), and completes any necessary data entry not covered by an electronic chain of custody.

The Screening step involves a preliminary assessment for drugs of abuse. Although Screening provides an economical way to eliminate drug use in the majority of samples, any positive results require confirmation to be admissible in court. All samples that are presumptive positives in Screening must undergo secondary confirmation.

If a sample tests presumptively positive during the Screening phase, additional hair is extracted from the initial sample and prepped for Extraction. Here, drugs are isolated from hair at substantially lower concentrations compared to other methods (e.g., urine or oral fluid), explaining why hair drug screening presents significant challenges.

Confirmation of positive screening results is performed with GC/MS, GC/MS/MS, or LC/MS/MS. All presumptive positive samples undergo washing before confirmation, as necessary. The complete lab procedure from Accessioning to Confirmation is evaluated under the CAP (College of American Pathologists) Hair designation and complies with ISO / IEC 17025 standards.

Advantages of hair drug testing:

  • Extended detection period: Hair drug tests can identify drug use for up to 90 days as opposed to urine tests, which offer a shorter detection period.
  • Hard to manipulate: Cheating a hair drug test is exceptionally challenging, enhancing the accuracy of the results.
  • Offers usage history: It can reveal a pattern of drug use over time, rather than just recent consumption.

Limitations:

  • Inability to detect recent consumption: Drugs typically become detectable in hair after about 5-7 days.
  • Expense: Hair drug tests tend to cost more than alternative drug testing approaches.
  • Result variability: Factors such as hair color and individual hair growth variations can influence drug metabolite concentrations within the hair.

Note: Although often termed "hair follicle tests", the actual analysis is performed on the hair strand, not the follicle beneath the scalp.

Why Hair Follicle Testing Beats Urine Testing

Hair follicle testing surpasses traditional urine tests due to its enhanced accuracy and reliability. In Springfield, KY, this method provides superior detection capabilities, making it an ideal choice for employers.

Compared with urine testing, hair follicle tests provide a broader detection window and minimize the chance of evasion, plus they offer more consistent results over time.

Choosing hair follicle testing over urine tests means benefiting from greater detection reliability and comprehensive assessment capabilities.

Extended Detection Window

The extended detection window of hair follicle tests catches drug use over a span of up to 90 days. In Springfield, KY, this method grants employers a broad insight into substance use history.

Allowing a more thorough overview, hair follicle testing extends its analysis capability beyond other methods, significantly benefiting long-term evaluations.

Employers receive a comprehensive background of an individual's drug history, ensuring informed decision-making based on extended detection.

Tamper-Resistant Testing Method

In Springfield, KY, hair follicle tests offer a tamper-resistant solution, minimizing the potential for sample adulteration.

Providing unquestionable results, hair follicle testing stands apart due to its inherent resistance to manipulation, ensuring trustworthy outcomes.

This method reduces doubts about sample validity, offering reliable assessments and maintaining high levels of confidence in test results.

Convenient Nationwide Locations

Accredited Drug Testing's network offers convenient nationwide and local facilities in Springfield, KY, making accessing testing services swift and hassle-free.

These strategically placed locations ensure that individuals can easily obtain necessary tests without the inconvenience of lengthy travel or waits.

Our widespread testing centers support quick service access, streamlining the testing process for immediate needs.

Simple, Confidential Process

In Springfield, KY, our hair follicle testing services promise a straightforward and confidential process, ensuring comfort and privacy at every step.

The entire process, from ordering to results, is designed for ease of use, ensuring that privacy concerns are seamlessly addressed.

Guaranteeing a confidential and user-friendly experience, this testing option appeals to those valuing simplicity and discretion.

Affordable, Fast Turnaround

With competitive pricing structures and swift result delivery, our hair follicle tests provide excellent value for businesses throughout Springfield, KY.

Affordable and efficient, this service offers high value for money and a promise of rapid testing results, ideal for companies of differing sizes and needs.

The quick turnaround and reasonable pricing of hair follicle testing maintain an attractive proposition for those seeking economic and rapid testing solutions.

Fast, Accurate, and Reliable Results

In Springfield, KY, hair follicle testing stands out for its speed and precision. Offering fast, accurate results, this testing method is highly efficient at detecting a range of substances, ensuring timely and dependable outcomes.

The capacity to provide rapid and precise results makes hair follicle testing a preferred choice for those seeking reliable drug use detection.

By focusing on quick and precise outcomes, hair follicle tests guarantee high levels of accuracy, useful in various contexts.

Compliance and Peace of Mind for Employers

Employers in Springfield, KY benefit from using hair follicle testing to maintain compliance with workplace drug-free policies.

Ensuring adherence to legal standards, this testing method helps in reducing legal vulnerabilities while enhancing workplace safety and employee welfare.

With a focus on compliance, hair follicle testing helps employers attain peace of mind, supporting their drug-free workplace requirements effectively.

What's New In Hair Follicle Drug Testing according to the Hair Drug Testing Experts

With its multidisciplinary nature, hair testing brings together researchers, scientists, and experts from various domains to develop innovative techniques, explore new methodologies, and address emerging concerns, responding to the ever-evolving challenges of drug abuse, therapeutic drug monitoring, and environmental exposures to pollutants.

The joint meeting of the Society of Hair Testing (SoHT) and the Italian Group of Forensic Toxicologists (GTFI) was organized in Verona, June 8–10, 2022, and hosted by the Legal Medicine team of the Verona University Hospital, directed by Franco Tagliaro together with Federica Bortolotti and Rossella Gottardo.

It served as a prominent platform for knowledge exchange and collaboration, facilitating groundbreaking advancements in hair testing and analysis. In this special issue of Drug Testing and Analysis, we aim to showcase the remarkable manuscripts presented at this significant event, shedding light on the latest research and highlighting the future directions in this field.

Novel Analytical Techniques: The Verona meeting witnessed the unveiling of cutting-edge analytical techniques, offering enhanced sensitivity, selectivity, and accuracy for drug testing. Remarkable advancements in sample preparation, detection, and identification of drug compounds have been presented. The manuscripts in this section presented novel approaches, such as multianalyte methods and on-line preparation which can enable rapid analysis and improve thorough forensic investigations.

Biomarkers and Pharmacokinetics: The identification and validation of reliable biomarkers and pharmacokinetic studies are fundamental aspects of drug testing and analysis. The impact of hair treatments on the oxidation/decomposition of drugs in hair was also explored, as the long-term fate of drugs incorporated in the keratin matrix. Furthermore, the SoHT meeting in Verona featured research on the identification and quantification of alcohol abuse biomarkers, their stability, and their correlation with exposure and other biomarkers.

Forensic Applications and Interpretation: One of the vital objectives of hair testing is its application in forensic investigations and legal proceedings. The SoHT meeting in Verona emphasized the significance of rigorous interpretation of analytical results and the need for standardized protocols. Manuscripts in this section present advancements in forensic toxicology, and have explored the challenges posed by emerging designer drugs and strategies to combat their proliferation. The pitfalls encountered when the exogenous/endogenous nature of a substance is questioned were presented and discussed. The special issue presents also manuscripts that delve into the ethical implications of drug testing in drug facilitated sexual assaults.

Clinical Applications of hair testing are witnessed by the insight on environmental tobacco smoke exposure, assessed by hair analysis in a population of students and the study of in utero-exposure to drugs of abuse through neonatal hair analysis. Exposure to drugs was also important in cases of child abuse/neglect, when the clinical purpose of hair testing merge with its forensic applications.

The legal framework surrounding hair testing and the achievement of guidelines to ensure fairness, accuracy, and confidentiality in drug testing practices have also been discussed,and the SoHT consensus on general hair testing and testing for drugs of abuse was presented at the meeting and published as a Letter to the Editor.

In conclusion, the special issue of Drug Testing and Analysis, featuring manuscripts from the SoHT–GTFI meeting in Verona 2022, reflects the remarkable advancements achieved in this field. The manuscripts provide valuable insights into novel analytical techniques, biomarkers, pharmacokinetics, forensic applications, and ethical considerations. As we embrace these advancements, we pave the way for a safer, healthier society, where drug testing and analysis serve as powerful tools in protecting public health and maintaining integrity in various sectors. The research presented in this special issue will undoubtedly shape the future of drug testing and analysis, fostering collaboration and inspiring further innovation in this crucial area of scientific inquiry.


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Accurate 5 panel drug testing with fast, confidential results—trusted by Springfield, KY employers and individuals nationwide.

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Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Springfield, KY workplace compliant.

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Certified DOT physicals with quick, reliable exams to keep Springfield, KY drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A hair follicle drug test analyzes a small sample of hair to detect a history of drug use over time. Because drug metabolites are incorporated into hair as it grows, this method can show patterns of use instead of just recent exposure. It is frequently used for legal and court requirements, long-term monitoring, and high-trust workforce screening.
Standard hair drug testing typically reflects drug use over approximately a 90-day period, based on the length of hair collected at the time of testing. Longer hair can sometimes reflect a longer time frame, and extremely short hair can reduce that window.
Hair drug testing commonly screens for marijuana/THC, cocaine, opiates/opioids, amphetamines/methamphetamines, and PCP. In many cases, additional expanded panels may be requested to include other substances.
Yes. If the donor's head hair is too short or unavailable, body hair may be collected as an alternative. The collector will document the source of the sample. This allows testing even for individuals with recently cut or shaved head hair.
Yes. Hair drug testing is commonly used in court, probation, custody, and compliance matters because the collection process follows a documented chain of custody and the analysis is performed by a certified laboratory. Results are provided in a form that can be reviewed by legal and administrative authorities.
Yes. Individuals, parents, attorneys, employers, and probation programs can all request hair drug testing. A doctor's order is not required. Results are confidential and released only to the authorized requesting party.
Hair testing provides a longer look-back period than urine testing, making it useful when you need to demonstrate ongoing abstinence or identify repeated/recent substance use patterns over time rather than just recent exposure. It is frequently selected for legal, compliance, and custody-related documentation.
A small section of hair is cut by a trained collector in a discreet, controlled manner. The sample is sealed, documented, and sent to the laboratory under established chain-of-custody procedures so that the results can be defended if they are ever challenged.

Customer Reviews

test was quick and easy

Company New Hire Employee Process for DOT Drug test was quick and easy. No need to make appt. All employee had to do is show prepaid qr code I sent to employee show ID and was set. Would Recommend.

Maria Lopez - 11/19/2024

This is by far the easiest

This is by far the easiest way to get my lab work ordered and paid for. The phone calls are short and to the point. They don’t try to push extra sales on you and when I walk in to the clinic I simply show my donor pass and with in a matter of minutes I’m done. I will continue to use ADT in the future.

Jason Jackson - 7/19/2025

ADT is truly the best

Where do I start?!! ADT is truly the best!! I had the pleasure of speaking with Tori today! She was excellent, professional and truly went above and beyond in her kind, compassionate care and commitment to providing me with the best service possible. I was in such a time crunch, but I was able to call, order the test, pay for it over the phone and get the test I needed within the hour. Not only did this put my mind at ease, they saved my job!! I am forever grateful for this team of hard workers, that care, with such great work ethics! Thank you from the very bottom of my heart!!! If you need a drug, alcohol, etc… screening, this team will be your lifesavers!!

Sarah Patterson - 4/8/2025

ADT was able to get me the best tests

Dealing with allegation’s of drug use that completely false is stressful, ADT was able to get me the best tests, going back the farthest in the same day with as little hassle as possible, I spoke with 1 person and wasn’t transferred once, and they took my payment, and got me my work order for a clinic as close as possible for the service I needed. 5*

Christopher Hansis - 12/19/2024

Had very fast and good service

Had very fast and good service, was able to get an appointment same day with the nearest facility. My questions were answered thoroughly but quick. Definitely recommend

Jacob Matzke - 11/19/2024

Fast and easy

It was fast and easy a pleasure to talk to the customer service people great service

Peter Villegas - 1/19/2025

Pretty Straightforward

It was a pretty straightforward process getting an appointment and making payment. Getting to the testing site was quick. I was in and out in no time.

Sadiat Ogunyemi - 12/19/2024

Fast and efficient service

Fast and efficient service for employers wanting to do pre employment drug screening that meets DOT requirements!!

Mary Thomas - 4/5/2025

phone support has been excellent

I have used ADT several times. Their phone support has been excellent, both when putting in the orders and when asking for help to interpret results.

Tee Ess - 12/19/2024

Extremely polite

They were very fast extremely polite walked you through every detail of the process if you had any questions they had no problems answering

Billy Williams - 2/19/2025


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