Urine| Hair Follicle | Oral Fluid | Blood | ETG
Hair testing is increasingly recognized for its efficacy in detecting substance and alcohol use. Hair captures a long-term history by embedding biomarkers in the growing fibers of the strand. Collected near the scalp, it can offer nearly a 3-month window for detecting alcohol and other substances. Hair samples are easy to collect, challenging to tamper with, and simple to transport.
A 1.5-inch sample consisting of approximately 200 hair strands (similar to the thickness of a #2 pencil) near the scalp will typically amount to 100mg—ideal for screening and confirmation. For EtG, supplemental tests, and analysis above 10 panels, a 150mg sample is suggested. It's advisable to measure the sample using a jeweler’s scale. If no scalp hair is present, an equivalent amount of body hair may be utilized. "Head hair" refers exclusively to scalp hair, while "body hair" refers to other types (facial, axillary, etc.).
Process Overview
The process of laboratory drug test result processing involves four principal steps: Accessioning, Screening, Extraction, and Confirmation.
Accessioning encompasses the initial step of processing a sample into the lab's system, ensuring proper sealing and shipment, allotting a random LAN (Laboratory Accessioning Number), and completing any extra data entry not available via an electronic chain of custody system.
Screening performs a prompt initial check for substance abuse. While cost-effective, a positive screen must be subsequently confirmed to be legally valid. Any presumptively positive samples in Screening demand a secondary confirmation.
If a sample indicates a presumptive positive at Screening, more hair is drawn from the initial sample and set for Extraction. Here, substances are extracted from hair at a significantly lower concentration than using other methods (like urine or oral fluid), which makes hair drug screening particularly complex.
For any positive screening outcomes, confirmation is done through GC/MS, GC/MS/MS, or LC/MS/MS. Before confirmation, all presumptive positives are washed as required. The comprehensive lab process from Accessioning to Confirmation is assessed under both the CAP (College of American Pathologists) Hair designation and ISO / IEC 17025 accreditation standards.
Advantages of hair drug testing:
Limitations:
Note: The test frequently called a "hair follicle test" actually examines the strand and not the follicle beneath the scalp.
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