Urine| Hair Follicle | Oral Fluid | Blood | ETG
The method of hair testing has gained recognition as an effective means for identifying drug and alcohol usage. Hair captures biomarkers in its growing fibers, offering an extended timeline for alcohol and drug detection. When taken near the scalp, a hair sample can reveal usage over a period of roughly three months. It is straightforward to collect, challenging to tamper with, and convenient for transportation.
A sample of 1.5 inches long with around 200 hair strands (similar in size to a #2 pencil) closest to the scalp will yield 100mg, which is the optimal sample size for both screening and confirmation. For the purposes of EtG, additional components, and/or tests exceeding 10 panels, a quantity of 150mg of the specimen is suggested. It is advisable to measure the sample on a jeweler’s scale. Should scalp hair not be available, the same amount of body hair may be taken. References to head hair pertain solely to scalp hair. Body hair encompasses all other types (such as facial, axillary, etc.).
Overview of the Process
The laboratory analysis of a drug test result involves four primary steps: Accessioning, Screening, Extraction, and Confirmation.
Accessioning is the initial step where a sample is processed into the laboratory's system. This step includes checking that the sample was sealed and transported correctly, assigning a unique LAN (Laboratory Accessioning Number), and performing any data entry not already covered by an electronic chain of custody system.
The Screening stage includes a preliminary check for drugs of abuse. While cost-efficient to rule out drug use in most samples, a positive screen needs confirmation to be legally admissible. Any samples that test as presumptively positive in Screening will require secondary confirmation.
In cases where a sample shows a presumptive positive during the Screening phase, additional hair is extracted from the initial sample and prepared for Extraction. In this phase, drugs are extracted from the hair at much lower concentrations compared to other methods (e.g., urine or oral fluid), making hair drug screening the most challenging methodology.
To confirm any positive result in Screening, GC/MS, GC/MS/MS, or LC/MS/MS methods are used. All samples that are presumptively positive are washed prior to confirmation if necessary. The process, from Accessioning to Confirmation, is reviewed under regulations set by both the CAP (College of American Pathologists) Hair designation and the ISO/IEC 17025 accreditation standards.
Benefits of hair drug testing:
Weaknesses:
Keep in mind: Often labeled as "hair follicle tests," these tests actually analyze the hair strand itself, not the follicle beneath the scalp.
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