If you are a US Department of Transportation regulated employer or if you hold a safety sensitive position covered by CFR 49 Part 40 Accredited Drug Testing provides DOT drug and alcohol testing, DOT Consortium enrollment, DOT physicals, FMCSA Clearinghouse registration and all other DOT required services at drug testing locations throughout the United States.
Our DOT specialists can schedule at a DOT drug testing location near you along with ensuring that you are in full compliance with all DOT requirements.We provide DOT drug and alcohol testing for pre-employment, Consortium/random selection, post accident, reasonable suspicion, return to duty and follow up testing.
Accredited Drug Testing has a certified DOT drug testing location near you. Call or schedule your DOT drug test online today.
All DOT drug tests are analyzed at SAMHSA - HHS certified laboratories for all DOT and federally regulated industries, in addition to review and verification by certified medical review officers (MRO's).
In accordance with 49 CFR Part 382
We help motor carriers and commercial drivers meet FMCSA drug and alcohol testing
regulations, ensuring compliance and promoting highway safety.
In accordance with 49 CFR Parts 4 & 16
Our services assist maritime employers in complying with USCG drug and alcohol testing
rules, protecting vessel safety and the health of crew members.
In accordance with 49 CFR Part 120
We provide drug and alcohol testing services that help aviation employers meet FAA
requirements, promoting safety in operations and maintenance.
In accordance with 49 CFR Part 219
We assist railroads and their employees in meeting FRA drug and alcohol testing rules,
supporting safety and reliability across the rail industry.
In accordance with 49 CFR Part 655
Our testing programs help transit employers comply with FTA drug and alcohol testing
regulations, safeguarding public transportation systems nationwide.
In accordance with 49 CFR Part 199
We support pipeline operators and hazardous material carriers with PHMSA-required drug
and alcohol testing services to ensure regulatory compliance.
Please select a state from the list below to find drug testing locations near you.
In accordance with CFR 49 Part 40, all DOT regulated employees including owner operators must be enrolled in a DOT random selection drug testing program at all times that they are performing a safety sensitive function. Accredited Drug Testing has mode specific DOT Consortiums and DOT stand alone pools which include certificates of enrollment compliance and C/TPA services when needed. Consortium enrollments are offered in one, two, three and five year terms and enrollment fees include all random tests if selected.
At Accredited Drug Testing, we provide comprehensive drug and alcohol testing services tailored to meet the needs of employers, individuals, legal professionals, and government agencies. With thousands of locations nationwide, we offer convenient, reliable, and certified testing solutions for pre-employment screenings, random drug tests, DOT-compliant tests, and court-ordered services.
Our testing options include DOT and non-DOT drug testing, alcohol testing, hair follicle testing, EtG alcohol screening, and lab-based urine analysis, all performed in accordance with federal and industry standards. We are highly experienced in fulfilling Department of Transportation (DOT) requirements for FMCSA, FAA, PHMSA, FTA, and FRA regulations. Additional services include consortium enrollment, random testing management, and DOT supervisor training programs to support full workplace compliance.
Accredited Drug Testing is committed to delivering fast, accurate results backed by outstanding customer service. All laboratory testing is processed by SAMHSA-certified labs, with Medical Review Officer (MRO) verification available when needed. Whether you're an employer striving for a drug-free workplace or an individual seeking testing for personal, professional, or legal reasons, our knowledgeable team is here to support you every step of the way.
Before starting any safety-sensitive position regulated by the Department of Transportation (DOT), employees must complete and pass a negative pre-employment drug test. While critical for public safety, this requirement can slow down the hiring process, create additional stress for candidates, and burden employers with administrative hurdles. Delays in testing can push back start dates, costing companies valuable time and resources — especially in industries already struggling with staffing shortages.
Additionally, candidates who fail to pass due to accidental exposure, confusion about substances like CBD, or inconsistencies between state and federal laws may face significant career setbacks. The financial and compliance costs for employers are also high, especially when trying to quickly fill open safety-sensitive roles without sacrificing quality or compliance.
That’s where we come in. Our streamlined DOT drug testing services help employers and candidates navigate the process with speed, clarity, and confidence. We offer same-day scheduling, thousands of nationwide testing locations, fast results, and full compliance support — making it easier for businesses to hire qualified, drug-free employees without unnecessary delays. Trust us to keep your operations running smoothly while maintaining the highest standards of DOT compliance.
Enter your zip code to find nearby test centers
We provide DOT Drug Testing, DOT Alcohol Testing, and other Services.
If you are a DOT regulated employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.
49 CFR Part 40, or Part 40, is the Department of Transportation (DOT) regulation that outlines procedures for workplace drug and alcohol testing in the transportation industry. It specifies how testing should be conducted, including collection and analysis of specimens, and how employees can return to safety-sensitive duties after a positive test or violation. Part 40 applies to all DOT-required testing, regardless of the mode of transportation.
Testing Procedures:
Outlines how to collect and analyze specimens, ensuring they are collected and analyzed in a manner consistent with DOT standards.
Confidentiality:
Specifies how test results and other information should be handled to protect the privacy of employees, including the roles and responsibilities of Medical Review Officers (MROs).
Return-to-Duty Process:
Describes the process for employees who have violated a DOT drug and alcohol regulation to return to safety-sensitive duties, often involving a Substance Abuse Professional (SAP) evaluation.
Laboratories:
Sets requirements for laboratories conducting DOT drug and alcohol tests, including certification requirements and reporting.
Service Agents:
Defines the roles and responsibilities of individuals or organizations that assist employers with drug and alcohol testing.
In essence, 49 CFR Part 40 is a comprehensive framework for ensuring that the transportation industry operates drug and alcohol free, promoting safety and protecting the public.
The DOT, along with FMCSA, adopted regulations requiring commercial motor vehicle (CMV) operators required to maintain a commercial driver's license (CDL) to be tested for alcohol and drugs. While you can hire various service agents to collect specimens, conduct laboratory analyses, medically review lab results and determine test outcomes; you cannot delegate your responsibility to comply with all applicable requirements and procedures of 49 CFR Part 40 and Part 382. This means that you are responsible for all actions of your employees, representatives, and agents.
DOT-regulated employers must enroll in a random drug and alcohol testing program to remain compliant with federal regulations. If your company operates in a safety-sensitive industry, participation in a compliant random testing program is *not optional* — it's mandatory. The Department of Transportation (DOT) requires that employees be randomly selected for testing throughout the year, ensuring that drug and alcohol use is deterred and public safety remains a top priority.
Each DOT agency has its own random testing rates. For example, the FMCSA currently requires random drug testing at a rate of 50% of covered employees annually and random alcohol testing at 10%. FAA employers must test 25% for drugs and 10% for alcohol, while FRA employees are tested at 50% for drugs and 25% for alcohol. FTA and PHMSA programs require 50% drug and 10% alcohol testing rates, and the USCG currently mandates 50% drug testing annually. (Please note: these rates are subject to official annual updates.)
At our company, we make compliance easy. Enrollment includes two random selections per year for each enrolled employee — helping you meet or exceed DOT minimum requirements. After the first two selections, additional selections are still handled for you, but a small fee per extra selection applies. This approach keeps your program affordable while ensuring you stay 100% compliant without any guesswork or gaps.
By partnering with us, you get more than just random selections. We handle all notifications, manage testing compliance paperwork, and ensure your drivers, pilots, or operators are covered — all with a simple, streamlined process. With access to thousands of testing sites nationwide and real-time support, our program saves you time, reduces your risk, and protects your business from costly DOT violations. Stay focused on running your operation — we’ll handle your random testing requirements with speed, accuracy, and compliance expertise.
