DOT Drug Testing - Gainesville, AL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 37 testing centers in the Gainesville area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

37 DOT Drug-Testing Centers in Gainesville

Minutes Away, Test Today

825 Northwest 23rd Ave., Unit 2

0.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
825 Northwest 23rd Ave., Unit 2
Gainesville, FL 32609

720 Sw 2nd Ave Ste 160a

1.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
720 Sw 2nd Ave Ste 160a
Gainesville, FL 32601

3925 Nw 43rd St

2.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3925 Nw 43rd St
Gainesville, FL 32606

4881 Nw 8th Ave Ste 2a

3.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4881 Nw 8th Ave Ste 2a
Gainesville, FL 32605

4343 W Newberry Rd Ste 10

3.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4343 W Newberry Rd Ste 10
Gainesville, FL 32607

812 Nw 57th St

3.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
812 Nw 57th St
Gainesville, FL 32605

6121 Nw 1st Pl

3.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6121 Nw 1st Pl
Gainesville, FL 32607

1131 Nw 64th Ter Ste A

3.84 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1131 Nw 64th Ter Ste A
Gainesville, FL 32605

3581 Sw Archer Rd Units 30 And 40

6.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3581 Sw Archer Rd Units 30 And 40
Gainesville, FL 32608

14819 Nw 140th St

12.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14819 Nw 140th St
Alachua, FL 32615

14417 Nw 152nd Ln

12.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14417 Nw 152nd Ln
Alachua, FL 32615

420 Ne Santa Fe Blvd

18.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
420 Ne Santa Fe Blvd
High Springs, FL 32643

1550 S Water St

22.36 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1550 S Water St
Starke, FL 32091

345 W Madison St

23.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
345 W Madison St
Starke, FL 32091

850 E Main St Attn Lab

25.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
850 E Main St Attn Lab
Lake Butler, FL 32054

128 Nw 137th Dr

29.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
128 Nw 137th Dr
Newberry, FL 32669

3470 Nw 57th Trl

32.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3470 Nw 57th Trl
Bell, FL 32619

1113 Nw 23rd Ave

33.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1113 Nw 23rd Ave
Chiefland, FL 32626

1109 Sw 10th St

35.84 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1109 Sw 10th St
Ocala, FL 34471

31 Sw 12th St

36.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
31 Sw 12th St
Ocala, FL 34471

2415 Sw College Rd

36.63 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
2415 Sw College Rd
Ocala, FL 34471

1834 Sw 1st Ave Ste 201

36.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1834 Sw 1st Ave Ste 201
Ocala, FL 34471

1015 Se 17th St Ste 200

36.77 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1015 Se 17th St Ste 200
Ocala, FL 34471

2685 Sw 32nd Place,

36.96 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2685 Sw 32nd Place,
Ocala, FL 34471

2100 Se 17th St Ste 902

37.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2100 Se 17th St Ste 902
Ocala, FL 34471

8602 Sw State Road 200, 103rd Street Plaza, Suite J

37.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8602 Sw State Road 200, 103rd Street Plaza, Suite J
Ocala, FL 34481

3824 W Us Highway 90

38.46 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3824 W Us Highway 90
Lake City, FL 32055

3824 E Us Highway 90

38.46 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3824 E Us Highway 90
Lake City, FL 32055

265 Sw Malone Street,suite 105

38.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
265 Sw Malone Street,suite 105
Lake City, FL 32025

4134 W Us Highway 90

38.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4134 W Us Highway 90
Lake City, FL 32055

260 S Marion Ave Bldg B Ste 125

39.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
260 S Marion Ave Bldg B Ste 125
Lake City, FL 32025

6160 Sw Highway 200

39.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6160 Sw Highway 200
Ocala, FL 34476

205 Zeagler Dr, Suite 201

39.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
205 Zeagler Dr, Suite 201
Palatka, FL 32177

325 E Baya Ave

39.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
325 E Baya Ave
Lake City, FL 32025

6500 Crill Ave

39.65 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6500 Crill Ave
Palatka, FL 32177

6100 Saint Johns Ave Ste C

39.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6100 Saint Johns Ave Ste C
Palatka, FL 32177

6101 Crill Ave

39.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6101 Crill Ave
Palatka, FL 32177

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 37 DOT drug testing locations to serve you in and around Gainesville, AL.

In Gainesville, AL, our DOT Drug Testing centers serve employers and single operators with necessary compliance services like DOT Drug Testing, DOT Alcohol Testing, and DOT Physicals. We ensure adherence to 49 CFR Part 40 across all DOT modes including FMCSA, FTA, FAA, USCG, FRA and PHMSA. Located conveniently near you, most services are available the same day to fit your schedule.

Choose your required DOT service or test at a nearby facility. Our process is efficient and straightforward, enabling easy arrangements for drug and alcohol testing locally, whether for personal or staff needs.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Gainesville, Alabama DOT Services

DOT Drug Testing & Services Available in Gainesville, AL:

In Gainesville, AL, our DOT services are designed to facilitate easy compliance with federal regulations. Accredited Drug Testing ensures a seamless process for employers and individuals in need of reliable testing services.

DOT Services in Gainesville, AL

Under DOT regulations, drug and alcohol screenings are mandatory to maintain employee safety and public trust. In Gainesville, Alabama, Accredited Drug Testing facilitates adherence through comprehensive testing services for all employees.

Employers in Gainesville are responsible for ensuring compliance with DOT standards to promote a drug-free work environment. Failure to comply can lead to severe penalties and safety risks.

Through efficient and accurate testing services, companies in Gainesville, AL, can ensure they meet federal requirements while promoting health and safety. Services are designed to streamline processes and ensure accuracy.

Accredited Drug Testing partners with businesses in Gainesville, Alabama, to uphold workplace integrity, safeguard employee wellbeing, and guarantee public safety through mandated DOT screenings.

FMCSA Information

FMCSA regulations in Gainesville, Alabama mandate regular drug and alcohol testing to ensure the safety of commercial drivers and the public.

Accredited Drug Testing provides FMCSA-compliant screening for drivers in Gainesville, AL, fostering a secure travel environment.

With stringent procedures, FMCSA testing in Gainesville aids companies in meeting federal requirements for driver safety and compliance.

USCG Information

At Accredited Drug Testing, USCG regulations are adhered to with precise drug and alcohol testing for maritime employees in Gainesville, AL.

Our USCG testing procedures in Gainesville ensure maritime safety, helping to maintain compliance with federal standards.

FAA Information

The FAA mandates regular testing for aviation employees in Gainesville, AL, ensuring operational safety. Accredited Drug Testing partners with local companies to meet these mandates.

Our FAA-compliant drug testing services in Gainesville safeguard air travel by ensuring personnel adherence to safety standards.

FRA Information

Accredited Drug Testing offers FRA-mandated testing in Gainesville, AL, assuring compliance for rail industry employees to promote safe railway operations.

FRA regulations are essential for public safety, with extensive testing services available across Gainesville, safeguarding operations.

FTA Information

The FTA enforces stringent testing in Gainesville, AL to maintain safety in public transit systems. Accredited Drug Testing provides necessary testing solutions.

Benefit from comprehensive FTA-compliant testing services in Gainesville, Alabama, safeguarding public transportation integrity.

PHMSA Information

PHMSA testing in Gainesville, AL targets compliance for pipeline workers, advocating for a drug-free environment to enhance safety.

Accredited Drug Testing delivers PHMSA drug screenings in Gainesville, Alabama, assisting companies in maintaining stringent safety standards.

Scheduling in Gainesville, AL

Scheduling a DOT drug or alcohol test in Gainesville, AL is simple. Contact us at (800) 221-4291 or use our online express registration, ensuring you complete the necessary information before heading to the center.

Upon entering your zip code, the system identifies the nearest testing center, delivering a registration form with address and instructions to your email. Just bring it with you—appointments are typically unnecessary if you register and pay upfront.

Our labs are SAMHSA-certified, assuring reliable results verified by licensed Medical Review Officers (MROs) within our team.

Besides Gainesville, AL, Accredited Drug Testing's facilities cover nearby metropolitan areas, extending your options for efficient service.

Whether traveling or at home, Accredited Drug Testing facilitates scheduling nationwide, ensuring testing accessibility anytime you need it.

Alabama Dept. of Transportation Updates

Gainesville, AL DUI Statistics

The Department of Transportation (DOT) closely monitors DUI incidents in Gainesville, AL and the surrounding area of Sumter County. Alabama has strict laws to combat DUI cases, reflecting in the enforcement measures in Gainesville. The town has implemented various initiatives to reduce DUI occurrences by focusing on public awareness campaigns and collaboration with local law enforcement agencies. Despite these efforts, the region still witnesses a notable number of DUI-related incidents annually. The DOT emphasizes the importance of continuous education to further reinforce safe driving practices and to decrease the rate of DUI cases across Alabama.

Drug-Involved Accidents in Sumter County, AL

Sumter County, AL, including areas such as Gainesville, has been part of statewide DOT efforts to address drug-involved accidents. Over recent years, Alabama has grappled with the challenge of reducing drug-related incidents on its roadways, implementing comprehensive testing and accident reporting mechanisms. Counselors and local law officials in Sumter County actively participate in state-sponsored educational programs aimed at reducing drug influence in driving. The synergy between the DOT and local organizations is crucial in mitigating the impact of driving under the influence of illegal substances in Alabama, striving for safer roads.

Marijuana Related Accidents in Gainesville, AL

Alabama, including Gainesville in Sumter County, is dealing with the growing issue of marijuana-related driving incidents. The Department of Transportation (DOT) in AL has been closely observing the uptick in accidents linked to marijuana consumption. With changing regulations around marijuana use in various parts of the U.S., Alabama remains committed to maintaining strict oversight to prevent impaired driving. Awareness campaigns target drivers across Sumter County, emphasizing drug-free operation of vehicles. Sumter County's local initiatives align with the DOT's state-wide strategies, aiming to curb marijuana-related accidents through education and rigorous law enforcement.

Gainesville Drug Testing

Quickly find trusted local drug testing centers in Gainesville, AL — fast, convenient, and reliable every time!

Gainesville DOT Drug Testing

Quickly find a local DOT drug testing center in Gainesville, AL — fast, reliable, convenient nationwide service!

Gainesville DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Gainesville, AL.

Gainesville Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Gainesville, AL.v

Gainesville Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Gainesville, AL locations—results you can trust, every time!

Gainesville Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Gainesville, AL.

Gainesville Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Gainesville, AL.

Gainesville 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Gainesville, AL employers and individuals nationwide.

Gainesville 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Gainesville, AL workplace and personal testing needs.

Gainesville On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Gainesville, AL workplace compliant.

Gainesville DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Gainesville, AL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291