DOT Drug Testing - Auburndale, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 38 testing centers in the Auburndale area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

38 DOT Drug-Testing Centers in Auburndale

Minutes Away, Test Today

125 Newbern Cir

0.25 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
125 Newbern Cir
Auburndale, FL 33823

430 E Derby Ave

1.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
430 E Derby Ave
Auburndale, FL 33823

1120 Havendale Blvd Nw

3.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1120 Havendale Blvd Nw
Winter Haven, FL 33881

62 Southampton Blvd

3.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
62 Southampton Blvd
Auburndale, FL 33823

550 Pope Ave Nw Ste 200

4.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
550 Pope Ave Nw Ste 200
Winter Haven, FL 33881

500 1st St N

4.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
500 1st St N
Winter Haven, FL 33881

400 1st St N

4.65 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
400 1st St N
Winter Haven, FL 33881

320 1st St S

4.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
320 1st St S
Winter Haven, FL 33880

575 E Central Ave

5.18 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-4:30 PM✓ Walk-in with Electronic Donor Pass
575 E Central Ave
Winter Haven, FL 33880

235 N Commonwealth Ave

7.76 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
235 N Commonwealth Ave
Polk City, FL 33868

2142 E Edgewood Dr

8.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2142 E Edgewood Dr
Lakeland, FL 33803

1801 Crystal Lake Dr

8.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1801 Crystal Lake Dr
Lakeland, FL 33801

1707 E Edgewood Dr

9.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1707 E Edgewood Dr
Lakeland, FL 33803

28055 Hwy 27

10.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
28055 Hwy 27
Dundee, FL 33838

1600 Lakeland Hills Blvd

10.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1600 Lakeland Hills Blvd
Lakeland, FL 33805

3037 Lakeland Hills Blvd Ste 1,

10.05 mi
🕑 Testing Hours: Mon–Sat: 6:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
3037 Lakeland Hills Blvd Ste 1,
Lakeland, FL 33805

3131 Lakeland Hills Blvd Ste 1

10.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3131 Lakeland Hills Blvd Ste 1
Lakeland, FL 33805

161 Webb Drive, Suite 300

10.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
161 Webb Drive, Suite 300
Davenport, FL 33837

2200 Osprey Blvd

11.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2200 Osprey Blvd
Bartow, FL 33830

3240 S Florida Ave Ste 101

11.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3240 S Florida Ave Ste 101
Lakeland, FL 33803

1750 N Broadway Ave

11.14 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1750 N Broadway Ave
Bartow, FL 33830

2209 North Blvd W Ste B

11.40 mi
🕑 Facility: Mon–Fri: 06:00 AM - 02:00 PM⚡ Drug Screens: Mon–Fri: 06:00 AM - 02:00 PM
2209 North Blvd W Ste B
Davenport, FL 33837

4226 Us Highway 98 N

11.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4226 Us Highway 98 N
Lakeland, FL 33809

5040 Us Highway 98 N

11.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5040 Us Highway 98 N
Lakeland, FL 33809

4710 S Florida Ave

11.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4710 S Florida Ave
Lakeland, FL 33813

1350 E Main St Ste C-1

11.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1350 E Main St Ste C-1
Bartow, FL 33830

3604 Harden Blvd

12.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3604 Harden Blvd
Lakeland, FL 33803

5676 S Florida Ave

13.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5676 S Florida Ave
Lakeland, FL 33813

5675 New Tampa Hwy Ste 1

15.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5675 New Tampa Hwy Ste 1
Lakeland, FL 33815

1485 Legends Blvd

17.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1485 Legends Blvd
Champions Gate, FL 33896

1424 State Road 60 E,

18.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1424 State Road 60 E,
Lake Wales, FL 33853

1324 State Road 60 E

18.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1324 State Road 60 E
Lake Wales, FL 33853

206 W Alexander St, Ste 2

21.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
206 W Alexander St, Ste 2
Plant City, FL 33563

1009 W Baker St

21.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1009 W Baker St
Plant City, FL 33563

413 N Alexander St

21.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
413 N Alexander St
Plant City, FL 33563

301 N Alexander St

21.58 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
301 N Alexander St
Plant City, FL 33563

802 W Dr Martin Luther King Jr Blvd Ste C

21.61 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 07:00 AM - 03:00 PM
802 W Dr Martin Luther King Jr Blvd Ste C
Plant City, FL 33563

607 S Alexander St Ste 107 110

21.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
607 S Alexander St Ste 107 110
Plant City, FL 33563

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 38 DOT drug testing locations to serve you in and around Auburndale, FL.

Located conveniently in Auburndale, FL, our DOT testing centers offer a range of essential services including drug and alcohol testing, physicals, and driver qualification reports, all aligning with 49 CFR Part 40 for FMCSA, FTA, FAA, USCG, FRA, and PHMSA regulations. Access to quick and efficient testing is just around the corner from your home or workplace with the availability of same-day service in many cases.

Choosing the DOT service or test you need and finding a nearby location is simple, whether it's for yourself, a staff member, or another person. Our efficient and approachable system makes arranging DOT testing near Auburndale seamless.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Auburndale, Florida DOT Services

DOT Drug Testing & Services Available in Auburndale, FL:

Our DOT testing services in Auburndale, FL, are tailored to meet diverse needs with accuracy and efficiency, ensuring compliance with federal regulations for both employers and individuals alike.

DOT Services in Auburndale, FL

Under DOT regulations, drug and alcohol testing is crucial to ensure safety in transportation. Employers in Auburndale, FL, must conduct regular screenings for compliance and to uphold a safe working environment.

Screening tests protect public safety and detect substance misuse among employees. Auburndale, Florida, employers must adhere to DOT protocols, ensuring all drivers are fit for duty.

Employers in Auburndale, FL, must be vigilant about DOT compliance, including random testing. This practice deters substance abuse and reinforces safety in transportation sectors.

For businesses in Auburndale, Florida, maintaining compliance with DOT regulations through systematic drug testing is essential to secure operations and keep the workforce healthy and efficient.

FMCSA Information

The FMCSA mandates drug and alcohol testing in Auburndale, FL, to enhance road safety by ensuring commercial vehicle operators are substance-free.

In Auburndale, Florida, FMCSA rules specify drug tests pre-employment and randomly for drivers to keep roads secure.

USCG Information

The USCG mandates drug tests in Auburndale, FL, for maritime workers to maintain safety and prevent substance-related mishaps.

In Auburndale, Florida, USCG rules enforce drug testing to ensure maritime operations run smoothly and securely.

FAA Information

FAA mandates in Auburndale, FL, require aviation employees to undergo drug tests to ensure the safety of flights.

Auburndale, Florida's FAA regulations demand random drug screenings for aviation staff to safeguard passenger and crew safety.

FRA Information

FRA rules in Auburndale, FL, obligate railroad employees to adhere to drug testing protocols for operational safety.

In Auburndale, Florida, the FRA enforces drug testing to confirm railroad workers are capable and substance-free.

FTA Information

FTA drug testing protocols in Auburndale, FL, ensure public transit workers operate in a drug-free and safe environment.

Auburndale, Florida’s FTA regulations require regular drug screenings to safeguard public transportation systems.

PHMSA Information

PHMSA regulations in Auburndale, FL, enforce drug testing for the safety and reliability of hazardous material transporters.

Drug testing under PHMSA rules in Auburndale, Florida, helps prevent incidents by confirming employees are substance-free.

Scheduling in Auburndale, FL

Need to book a DOT drug or alcohol test in Auburndale, FL? Dial us at (800) 221-4291 or use our express online scheduling. Complete the registration to secure your test appointment.

The provided zip code helps find the nearest testing site in Auburndale, Florida. You'll receive a donor pass containing the center's details and guidelines. Simply bring it with you; pre-registration and payment are required.

All our labs hold SAMHSA certification, and test results are validated by our trained Medical Review Officers.

Besides DOT testing in Auburndale, we have numerous centers throughout the area and adjacent cities.

Traveling? Accredited Drug Testing has centers all over the country, ensuring testing is available wherever you are at any time!

Florida Dept. of Transportation Updates

DUI Statistics in Auburndale, FL

In Auburndale, Florida, or within the encompassing Polk County, DUI-related incidents represent a significant concern for the Department of Transportation. Florida consistently ranks high for DUI arrests, and Auburndale is no exception. The Florida Department of Highway Safety and Motor Vehicles (FLHSMV) reports that in Polk County, a considerable number of traffic incidents each year involve alcohol impairment. Efforts to curb these rates include DUI checkpoints and public awareness campaigns across Florida. Despite these efforts, DUI remains a critical issue in Auburndale, with law enforcement continuing to emphasize the dangers of driving under the influence in Florida.

Drug-Involved Accidents in Auburndale, FL

Within Auburndale, FL, and broader Polk County, drug-related accidents are a growing concern for the Department of Transportation. The use of illegal substances and misuse of prescription drugs poses substantial risks on Florida's roadways. The FLHSMV highlights that in Polk County, drug impairment contributes significantly to traffic accidents annually. Auburndale law enforcement collaborates with state initiatives to reduce these incidents through education and enforcement. Florida's commitment to reducing drug-involved accidents involves multi-agency task forces aiming to protect the safety of Floridians on the roads.

Marijuana-Related Accidents in Auburndale, FL

In Auburndale, Florida, and its surrounding Polk County region, marijuana-related traffic accidents are a critical area of concern for the Department of Transportation. Following changes in marijuana legislation across the United States, Florida sees a notable number of incidents where marijuana use is a factor. The FLHSMV continues to monitor and report on these occurrences within Polk County. Law enforcement in Auburndale remains vigilant in identifying and addressing the challenges posed by marijuana impairment. Various campaigns and educational programs are underway in Florida to mitigate the impact of marijuana on driving safety.

Auburndale Drug Testing

Quickly find trusted local drug testing centers in Auburndale, FL — fast, convenient, and reliable every time!

Auburndale DOT Drug Testing

Quickly find a local DOT drug testing center in Auburndale, FL — fast, reliable, convenient nationwide service!

Auburndale DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Auburndale, FL.

Auburndale Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Auburndale, FL.v

Auburndale Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Auburndale, FL locations—results you can trust, every time!

Auburndale Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Auburndale, FL.

Auburndale Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Auburndale, FL.

Auburndale 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Auburndale, FL employers and individuals nationwide.

Auburndale 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Auburndale, FL workplace and personal testing needs.

Auburndale On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Auburndale, FL workplace compliant.

Auburndale DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Auburndale, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

Very easy to get scheduled

Very easy to get scheduled and easy process once checked in and the staff were all extremely friendly! I would recommend this company!

Ronickia Moore - 11/19/2024

phone support has been excellent

I have used ADT several times. Their phone support has been excellent, both when putting in the orders and when asking for help to interpret results.

Tee Ess - 12/19/2024

Super helpful

Customer service was surprisingly super helpful and so nice. She was able to get me in at a local location right away the same day. She helped me figure out exactly what I needed. Very impressed.

Brook - 9/19/2024

Had very fast and good service

Had very fast and good service, was able to get an appointment same day with the nearest facility. My questions were answered thoroughly but quick. Definitely recommend

Jacob Matzke - 11/19/2024

Very Fun and Easy

Trish last week and Tatiana this week, very fun and easy folks to deal with. Well be using them more and more in the future.

Tom O - 12/19/2024

Quick, Polite, Informative

Everyone has been quick to respond, polite, informative. It's been easy to order tests. I would recommend them to anyone to use.

April Rogers - 2/15/2025

Everything was great

Everything was great, the staff was very polite. Thank you.

Olga Petrova - 9/19/2024

I love the customer service

I love the customer service. The lady was patient enough to help me book my appointment with ease and ensuring I get the location that's best for me. Keep up the good work!

Elizabeth Chiaka - 9/19/2024

Great service

Great service easy use quick processing! Need a hair follicle for my DCFS case , quick and convenient!

Jesse Dupre - 4/12/2025

ADT was able to get me the best tests

Dealing with allegation’s of drug use that completely false is stressful, ADT was able to get me the best tests, going back the farthest in the same day with as little hassle as possible, I spoke with 1 person and wasn’t transferred once, and they took my payment, and got me my work order for a clinic as close as possible for the service I needed. 5*

Christopher Hansis - 12/19/2024


(800) 221-4291