DOT Drug Testing - Berkeley, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 36 testing centers in the Berkeley area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

36 DOT Drug-Testing Centers in Berkeley

Minutes Away, Test Today

3037 Landover Blvd

2.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3037 Landover Blvd
Spring Hill, FL 34608

7007 Nightwalker Rd,

2.82 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-3:00 PM • Sat: 6:30 AM-10:30 AM✓ Walk-in with Electronic Donor Pass
7007 Nightwalker Rd,
Brooksville, FL 34613

4112 Mariner Blvd

2.92 mi
🕑 Testing Hours: Mon–Sat: 9:00 AM-9:00 PM • Sun: 10:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
4112 Mariner Blvd
Spring Hill, FL 34609

3041 Landover Blvd.,

2.94 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-2:00 PM • Sat: 6:00 AM-10:00 AM✓ Walk-in with Electronic Donor Pass
3041 Landover Blvd.,
Spring Hill, FL 34608

12120 Cortez Blvd

3.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12120 Cortez Blvd
Brooksville, FL 34613

12220 Cortez Blvd

3.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12220 Cortez Blvd
Brooksville, FL 34613

12114 Cortez Blvd #205

4.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12114 Cortez Blvd #205
Brooksville, FL 34613

170 Mariner Blvd., Unit 7-a

5.24 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
170 Mariner Blvd., Unit 7-a
Spring Hill, FL 34609

10441 Quality Dr Ste 105

5.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10441 Quality Dr Ste 105
Spring Hill, FL 34609

40 Seven Hills Dr,

5.70 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
40 Seven Hills Dr,
Spring Hill, FL 34609

11123 County Line Rd

5.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11123 County Line Rd
Spring Hill, FL 34609

14000 Fivay Rd Injury Care Only

8.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14000 Fivay Rd Injury Care Only
Hudson, FL 34667

17240 Cortez Blvd

9.31 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
17240 Cortez Blvd
Brooksville, FL 34601

14100 Fivay Rd Ste 140

11.54 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14100 Fivay Rd Ste 140
Hudson, FL 34667

13944 Lakeshore Blvd Ste D

11.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
13944 Lakeshore Blvd Ste D
Hudson, FL 34667

20205 Cortez Blvd

11.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
20205 Cortez Blvd
Brooksville, FL 34601

7459 State Road 52,

13.30 mi
🕑 Testing Hours: Mon–Fri: 5:30 AM-2:00 PM • Sat: 6:00 AM-10:30 AM✓ Walk-in with Electronic Donor Pass
7459 State Road 52,
Hudson, FL 34667

11528 Us Highway 19

14.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11528 Us Highway 19
Port Richey, FL 34668

9328 Us Highway 19

16.24 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9328 Us Highway 19
Port Richey, FL 34668

5400 School Rd

20.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5400 School Rd
New Port Richey, FL 34652

5124 Us Highway 19

20.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5124 Us Highway 19
New Port Richey, FL 34652

10730 State Road 54

20.61 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10730 State Road 54
Trinity, FL 34655

3956 S Suncoast Blvd

20.86 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
3956 S Suncoast Blvd
Homosassa, FL 34448

4741 Us Highway 19,

20.87 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4741 Us Highway 19,
New Port Richey, FL 34652

8745 State Road 54

20.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8745 State Road 54
New Port Richey, FL 34655

31075 Cortez Blvd

21.83 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
31075 Cortez Blvd
Brooksville, FL 34602

2404 Us Highway 19

23.24 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2404 Us Highway 19
Holiday, FL 34691

5504 Gateway Blvd

23.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5504 Gateway Blvd
Wesley Chapel, FL 33544

22945 State Road 54

23.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
22945 State Road 54
Lutz, FL 33549

27421 State Road 54

24.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
27421 State Road 54
Wesley Chapel, FL 33544

24420 State Road 54

24.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
24420 State Road 54
Lutz, FL 33559

659 Ne Hwy 19 Ste 1

24.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
659 Ne Hwy 19 Ste 1
Crystal River, FL 34429

11371 N Williams St Unit 4

24.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11371 N Williams St Unit 4
Dunnellon, FL 34429

1907 Hwy 44 W

25.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1907 Hwy 44 W
Inverness, FL 34453

34498 Cortez Blvd

25.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
34498 Cortez Blvd
Ridge Manor, FL 33523

26827 Foggy Creek Rd, Bldg. 6 Suite 102

25.34 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-2:00 PM • Sat: 7:30 AM-11:30 AM✓ Walk-in with Electronic Donor Pass
26827 Foggy Creek Rd, Bldg. 6 Suite 102
Wesley Chapel, FL 33544

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 36 DOT drug testing locations to serve you in and around Berkeley, FL.

Our Berkeley, FL facilities offer comprehensive DOT services including Drug and Alcohol Testing, Physicals, and Random pool enrollments for employers and individuals to meet 49 CFR Part 40 compliance for FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Conveniently located for quick access, most tests offer same-day service.

Choose your required DOT service or test, and find a nearby facility for yourself, employees, or others. Our simple process assists you in scheduling DOT drug testing near Berkeley with ease.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Berkeley, Florida DOT Services

DOT Drug Testing & Services Available in Berkeley, FL:

Accredited Drug Testing offers comprehensive DOT services in Berkeley, FL, ensuring compliance with all regulations. We provide efficient and dependable testing options to meet employer and operator needs.

DOT Services in Berkeley, FL

DOT regulations ensure employee safety and prevent substance abuse in transportation roles. Compliance with these rules in Berkeley, FL is crucial for public safety and operator accountability.

Regular drug and alcohol screenings are mandatory under DOT regulations, promoting a secure work environment. Employers in Berkeley, Florida uphold these standards diligently to enhance productivity and public safety.

Adherence to 49 CFR Part 40 by Berkeley, FL businesses ensures consistent and fair drug testing, contributing to a safer transit system by promoting responsible behavior among operators.

Berkeley, Florida's commitment to DOT standards fosters a culture of safety in transportation industries, minimizing risks associated with drug abuse and ensuring robust safety protocols.

FMCSA Information

In Berkeley, FL, FMCSA mandates rigorous drug and alcohol testing to maintain safety for commercial drivers, ensuring workplace security and public trust.

FMCSA regulations enforce random screenings, reducing the risk of substance abuse among truck and bus operators in Berkeley, FL.

USCG Information

USCG enforces drug testing protocols for maritime workers in Berkeley, FL, maintaining competency and public safety on waterways.

Berkeley, Florida's maritime sectors comply with USCG testing rules, ensuring safety and efficient operations in marine environments.

FAA Information

FAA regulations in Berkeley, FL demand drug testing for aviation personnel, safeguarding passenger safety and upholding high performance standards.

Berkeley, Florida aviation employees adhere to FAA rules for routine screenings, ensuring competence and security within the industry.

FRA Information

By following FRA guidelines, Berkeley, FL rail industry workers are subject to drug testing, enhancing safety and preventing accidents linked to substance abuse.

FRA mandates in Berkeley, Florida are vital in maintaining clear operational standards and safety in rail services.

FTA Information

FTA drug testing policies in Berkeley, FL ensure transit workers serve the public safely, promoting trust and reliability in transportation services.

Comprehensive FTA screenings in Berkeley, Florida's transit systems prevent impairments that could endanger passengers.

PHMSA Information

PHMSA ensures hazardous materials transportation in Berkeley, FL adheres to strict drug testing, promoting safe handling and minimizing risk factors.

Berkeley, FL employees involved in hazardous materials transport comply with PHMSA guidelines, ensuring sober operation at all times.

Scheduling in Berkeley, FL

To arrange any DOT drug or alcohol test in Berkeley, FL, contact our scheduling at (800) 221-4291 or use our online portal by registering and selecting your test. Complete registration before visiting a center.

The zip code you provide helps find the nearest Berkeley, FL test center for your chosen test. A donor pass with center details will be emailed. No prior appointment is generally needed but register and pay at the time of registration.

Our labs are SAMHSA-certified with results validated by licensed Medical Review Officers in-house, ensuring dependable testing results.

Apart from Berkeley, FL, Accredited Drug Testing offers numerous centers throughout the metropolitan area and its neighboring cities.

With multiple centers nationally, Accredited Drug Testing accommodates tests at any location or time, ensuring reliability even on the go.

Florida Dept. of Transportation Updates

City of Berkeley, FL DUI Statistics

Currently, there are no specific DUI statistics available exclusively for the City of Berkeley, FL, or the county it resides in. However, the state of Florida experiences a significant number of DUI incidents annually. In 2020, the Florida Department of Highway Safety and Motor Vehicles reported over 32,000 driving under the influence (DUI) arrests statewide. This highlights a substantial issue of impaired driving across Florida, which likely impacts cities like Berkeley, FL. The Florida Department of Transportation continues to invest in education and enforcement initiatives to combat DUI incidents, focusing on safeguarding citizens and promoting road safety.

City of Berkeley, FL Drug Involved Accidents

While specific data regarding drug-involved accidents in the City of Berkeley, FL, is unavailable, Florida state statistics provide some context. The Florida Department of Highway Safety and Motor Vehicles reports that in 2020, there were approximately 5,000 crashes statewide involving drugs, contributing to both injuries and fatalities. These incidents often involve substances that impair cognitive and motor functions, impacting driver judgment and reaction times. The state recognizes the challenge posed by drug-related road incidents and supports initiatives aimed at prevention and education, striving to reduce such occurrences in all cities, including Berkeley, FL.

City of Berkeley, FL Marijuana Related Accidents

There is no direct data for marijuana-related accidents specifically in the City of Berkeley, FL. Nonetheless, marijuana has been increasingly involved in traffic incidents as its use becomes more widespread. In Florida, the rise in marijuana usage presents a growing concern for traffic safety authorities. According to state-level data, marijuana is often detected in drivers involved in accidents. This has prompted the Florida Department of Transportation to implement strategies focusing on public awareness and legal measures targeting impaired driving, including marijuana consumption. Such efforts are crucial for protecting road safety in the City of Berkeley, FL, and beyond.

Berkeley Drug Testing

Quickly find trusted local drug testing centers in Berkeley, FL — fast, convenient, and reliable every time!

Berkeley DOT Drug Testing

Quickly find a local DOT drug testing center in Berkeley, FL — fast, reliable, convenient nationwide service!

Berkeley DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Berkeley, FL.

Berkeley Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Berkeley, FL.v

Berkeley Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Berkeley, FL locations—results you can trust, every time!

Berkeley Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Berkeley, FL.

Berkeley Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Berkeley, FL.

Berkeley 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Berkeley, FL employers and individuals nationwide.

Berkeley 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Berkeley, FL workplace and personal testing needs.

Berkeley On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Berkeley, FL workplace compliant.

Berkeley DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Berkeley, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

Everything was great

Everything was great, the staff was very polite. Thank you.

Olga Petrova - 9/19/2024

Very Fun and Easy

Trish last week and Tatiana this week, very fun and easy folks to deal with. Well be using them more and more in the future.

Tom O - 12/19/2024

Fast service and friendly staff!

Fast service and friendly staff! Could not recommend enough for drug testing services.

Habib Malik - 4/12/2025

Friendly and Knowledgeable

I reached out Accredited Drug Testing/Secure Screenings to partner for my drug testing business (Discovery Diagnostics Columbia SC) and the process was extremely easy. I worked with Sicilia who was amazing. She was friendly & knowledgeable, and answer all of my questions with ease.

Kendra Harley - 3/26/2025

Pretty Straightforward

It was a pretty straightforward process getting an appointment and making payment. Getting to the testing site was quick. I was in and out in no time.

Sadiat Ogunyemi - 12/19/2024

Reception is kind and helpful

Reception is kind and helpful , everything quick and simple , best urgent care

Yusuf Narmuradov - 11/19/2024

Great experience

Great experience and great lab. Customer service agent was very helpful.

Jenna Bee - 2/19/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

Super Easy!

Super easy to set up an appointment through the phone. They walked me through all the steps and found me a location right near my house. Would definitely recommend!

Aldita Lumaj - 1/17/2025

Quick, knowledgeable and courteous

Quick, knowledgeable and courteous Janelle worked diligently to support me. I am recommending their services to anyone looking.

Greensboro Joseph - 11/19/2024


(800) 221-4291