DOT Drug Testing - Casselberry, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 32 testing centers in the Casselberry area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

32 DOT Drug-Testing Centers in Casselberry

Minutes Away, Test Today

1239 Sr 436 Walmart #101

0.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1239 Sr 436 Walmart #101
Casselberry, FL 32707

238 Wilshire Blvd Ste 120

1.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
238 Wilshire Blvd Ste 120
Casselberry, FL 32707

705 W State Road 434 Ste J & K

2.05 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
705 W State Road 434 Ste J & K
Longwood, FL 32750

855 S Us Highway 17 92

2.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
855 S Us Highway 17 92
Longwood, FL 32750

1060 West Sr 434, Ste 116

2.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1060 West Sr 434, Ste 116
Longwood, FL 32750

512 E Altamonte Dr Ste 1000

2.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
512 E Altamonte Dr Ste 1000
Altamonte Springs, FL 32701

499 E Central Pkwy Ste 245

2.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
499 E Central Pkwy Ste 245
Altamonte Springs, FL 32701

393 Center Pointe Cir Ste 1457

3.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
393 Center Pointe Cir Ste 1457
Altamonte Springs, FL 32701

691 Douglas Ave

3.79 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
691 Douglas Ave
Altamonte Springs, FL 32714

270 Northlake Blvd Ste 1004

3.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
270 Northlake Blvd Ste 1004
Altamonte Springs, FL 32701

440 W State Road 436

4.38 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
440 W State Road 436
Altamonte Springs, FL 32714

901 N Lake Destiny Rd Ste 400

4.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
901 N Lake Destiny Rd Ste 400
Maitland, FL 32751

5355 Red Bug Lake Rd

4.94 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
5355 Red Bug Lake Rd
Winter Springs, FL 32708

3099 Aloma Ave

5.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3099 Aloma Ave
Winter Park, FL 32792

7457 Aloma Ave Ste 301

5.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7457 Aloma Ave Ste 301
Winter Park, FL 32792

5931 Aloma Ave Ste 100

5.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5931 Aloma Ave Ste 100
Winter Park, FL 32792

136 Parliament Loop Ste 1020

5.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
136 Parliament Loop Ste 1020
Lake Mary, FL 32746

7480 Aloma Ave

5.35 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7480 Aloma Ave
Winter Park, FL 32792

5931 Brick Court Suite 100

5.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5931 Brick Court Suite 100
Winter Park, FL 32792

611 N Wymore Rd Ste 220

5.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
611 N Wymore Rd Ste 220
Winter Park, FL 32789

501 N Orlando Ave Ste 227

5.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
501 N Orlando Ave Ste 227
Winter Park, FL 32789

1850 Lee Rd Ste 216

5.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1850 Lee Rd Ste 216
Winter Park, FL 32789

1850 Lee Rd Ste 300

5.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1850 Lee Rd Ste 300
Winter Park, FL 32789

4122 Metric Dr Ste 800

5.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4122 Metric Dr Ste 800
Winter Park, FL 32792

2540 Lee Rd

5.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2540 Lee Rd
Winter Park, FL 32789

1950 W Sr 426 Ste 108,

6.25 mi
🕑 Testing Hours: Mon–Fri: 11:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
1950 W Sr 426 Ste 108,
Oviedo, FL 32765

8010 Red Bug

6.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8010 Red Bug
Oviedo, FL 32765

1410 W Broadway St Ste 108

6.35 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1410 W Broadway St Ste 108
Oviedo, FL 32765

1511 E State Road 434 Ste 2001

6.41 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1511 E State Road 434 Ste 2001
Winter Springs, FL 32708

1338 Pleasant Oak Ln

6.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1338 Pleasant Oak Ln
Orlando, FL 32804

5021 Eggleston Ave Ste A

6.90 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5021 Eggleston Ave Ste A
Orlando, FL 32804

324 E Par St.., Ste 100,

7.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
324 E Par St.., Ste 100,
Orlando, FL 32804

Serving All DOT modes
DOT Drug Testing, DOT Consortium,
FMCSA Clearinghouse, Physicals

We have 32 DOT drug testing locations to serve you in and around Casselberry, FL.

Our DOT facilities in Casselberry, FL and nearby offer a range of DOT services for compliance with 49 CFR Part 40. This includes drug and alcohol testing, physicals, and consortium enrollments for FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Conveniently located near your home or office, most services are available same-day.

Choose your DOT service or test, select a convenient location, and complete the process for yourself or your employees. Our streamlined service makes scheduling in Casselberry effortless.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Casselberry, Florida DOT Services

DOT Drug Testing & Services Available in Casselberry, FL:

In Casselberry, FL, Accredited Drug Testing provides comprehensive DOT services, ensuring compliance with federal regulations. Whether for individual or organizational needs, our streamlined process guarantees efficient and accurate testing.

DOT Services in Casselberry, FL

DOT regulations mandate comprehensive drug and alcohol screening to guarantee employee safety in Casselberry, FL. Compliance with these regulations minimizes risks in transportation sectors and promotes public safety.

In Casselberry, FL, DOT protocols require random drug testing, post-accident screening, and reasonable suspicion testing for safety-sensitive employees, ensuring adherence to federal safety standards.

Casselberry, Florida emphasizes pre-employment drug screenings for DOT-regulated employees to ensure the safety and reliability of those operating within transportation industries.

Adherence to DOT regulations in Casselberry, FL includes implementing educational programs to inform employees about the dangers and consequences of substance misuse in safety-sensitive positions.

FMCSA Information

FMCSA rules in Casselberry, FL require strict adherence to alcohol and drug testing protocols for commercial drivers and operators of motor vehicles to promote public safety and prevent accidents.

Drivers in Casselberry, Florida must undergo regular drug testing as per FMCSA regulations, ensuring that all safety-sensitive duties are performed by qualified personnel.

USCG Information

USCG requirements in Casselberry, FL mandate rigorous drug and alcohol testing for maritime employees to ensure navigation safety and mitigate risks on waterborne vessels.

Maritime operators in Casselberry, Florida must adhere to USCG testing guidelines to comply with federal safety regulations and protect the marine environment.

FAA Information

In Casselberry, FL, FAA regulations enforce drug and alcohol testing for aviation employees, ensuring safety in the skies through rigorous compliance programs.

Safety-sensitive aviation roles in Casselberry, Florida require regular FAA-compliant drug screenings to maintain the highest safety standards within the industry.

FRA Information

FRA mandates in Casselberry, FL enforce comprehensive drug and alcohol testing, crucial for rail safety and the prevention of accidents related to substance misuse.

Railroad employees in Casselberry, Florida must conform to FRA drug testing standards to ensure operational safety and compliance with federal regulations.

FTA Information

FTA mandates for Casselberry, FL require transit employees to partake in regular drug and alcohol testing, promoting a secure public transportation environment.

Compliance with FTA testing protocols in Casselberry, Florida ensures the safety and reliability of public transit operators and associated personnel.

PHMSA Information

PHMSA regulations in Casselberry, FL focus on maintaining the integrity of transportation-related infrastructure through regular drug and alcohol testing of employees in safety-sensitive roles.

Casselberry, Florida-based employees performing duties under PHMSA must comply with stringent drug and alcohol testing to prevent hazards and ensure public safety.

Scheduling in Casselberry, FL

For any DOT drug or alcohol test in Casselberry, FL, contact us at (800) 221-4291 or use our express online system by choosing your test and filling out the registration info. Register before heading to the center.

Your zip code helps find the nearest Casselberry, FL testing center. You'll receive a donor pass with details via email—bring it along or on your smartphone. Usually, no appointment is needed, but registration and payment are required first.

Our SAMHSA-certified labs ensure test results are validated by our licensed Medical Review Officers (MRO).

Accredited Drug Testing services extend beyond Casselberry, FL, covering the entire metropolitan area and nearby cities for DOT drug and alcohol tests.

Accredited Drug Testing has testing centers nationwide, ensuring you can schedule tests across all cities without hassle, any time, anywhere!

Florida Dept. of Transportation Updates

Casselberry, FL DUI Statistics

Casselberry, located in Seminole County, Florida, has seen significant efforts by state and local authorities to address the issue of driving under the influence (DUI). Florida law enforcement has been active in conducting DUI checkpoints and awareness campaigns to reduce such incidents. In recent years, the county has reported a gradual decrease in DUI-related incidents, reflecting the efficacy of these interventions. However, despite these efforts, Seminole County still reports a concerning number of DUI cases annually, thus highlighting the continued need for public education and law enforcement vigilance to combat impaired driving and ensure road safety in the Sunshine State.

Casselberry, FL Drug-Involved Accident Statistics

Drug-involved accidents are a significant concern in Casselberry, FL, and the greater Seminole County area. The Florida Department of Transportation has highlighted the role of various substances, including prescription drugs, in increasing accident risks. Efforts by Florida law enforcement, community health programs, and public awareness campaigns aim to decrease these incidents. The state of Florida acknowledges the complexity of drug-related traffic incidents and prioritizes cross-agency collaboration to address this public safety issue. Despite efforts, drug-involved accidents remain a notable challenge for authorities in the region, necessitating ongoing attention and resources.

Casselberry, FL Marijuana-Related Accident Statistics

In recent years, Casselberry, FL, part of Seminole County, has experienced a noticeable impact from the increased use of marijuana and its implications for traffic safety. With Florida’s evolving legal landscape concerning marijuana, local law enforcement and the Florida Department of Transportation are keenly observing its effects on road safety. There is a particular focus on ensuring that drivers understand the risks associated with driving under the influence of marijuana. While the state of Florida continues to refine its policies and preventative measures, the presence of marijuana-related traffic incidents remains a critical area of concern for the safety of Casselberry residents and the broader community.

Casselberry Drug Testing

Quickly find trusted local drug testing centers in Casselberry, FL — fast, convenient, and reliable every time!

Casselberry DOT Drug Testing

Quickly find a local DOT drug testing center in Casselberry, FL — fast, reliable, convenient nationwide service!

Casselberry DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Casselberry, FL.

Casselberry Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Casselberry, FL.v

Casselberry Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Casselberry, FL locations—results you can trust, every time!

Casselberry Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Casselberry, FL.

Casselberry Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Casselberry, FL.

Casselberry 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Casselberry, FL employers and individuals nationwide.

Casselberry 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Casselberry, FL workplace and personal testing needs.

Casselberry On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Casselberry, FL workplace compliant.

Casselberry DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Casselberry, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

Fantastic Experience

Fantastic experience from the beginning. Jocelyn was very helpful to me as someone who doesn’t have much experience in needing a test. They were very helpful and walked me through the entire process start to finish. Thank you ADT!

Riley Wilson - 2/18/2025

Clear and Competent

I was so surprised with the quality of customer service I received today when I called Accredited Drug Testing. The representative was so clear and competent. She was able to reschedule an appointment at my preferred location. The representative whose name is Justice, was so polite and competent. She expedited the whole process. It was a relief to me.

Rachel Padian - 9/6/2024

Everything was great

Everything was great, the staff was very polite. Thank you.

Olga Petrova - 9/19/2024

Smooth and Seamless

I use their service for new hire and DOT employee's. Spoke with Taisha Walker this morning, and she was very helpful. She made the process smooth and seamless.

Christina Galdos - 3/9/2025

This is by far the easiest

This is by far the easiest way to get my lab work ordered and paid for. The phone calls are short and to the point. They don’t try to push extra sales on you and when I walk in to the clinic I simply show my donor pass and with in a matter of minutes I’m done. I will continue to use ADT in the future.

Jason Jackson - 7/19/2025

A very nice company

A very nice company of expertise. The staff will go above to help you out of your situation in a timely matter 👏

Tam Jack - 2/19/2025

Explained everything so no questions

Torin was great. Explained everything so no questions

Tony Lombardo - 4/18/2025

ADT is truly the best

Where do I start?!! ADT is truly the best!! I had the pleasure of speaking with Tori today! She was excellent, professional and truly went above and beyond in her kind, compassionate care and commitment to providing me with the best service possible. I was in such a time crunch, but I was able to call, order the test, pay for it over the phone and get the test I needed within the hour. Not only did this put my mind at ease, they saved my job!! I am forever grateful for this team of hard workers, that care, with such great work ethics! Thank you from the very bottom of my heart!!! If you need a drug, alcohol, etc… screening, this team will be your lifesavers!!

Sarah Patterson - 4/8/2025

Super helpful

Customer service was surprisingly super helpful and so nice. She was able to get me in at a local location right away the same day. She helped me figure out exactly what I needed. Very impressed.

Brook - 9/19/2024

Reception is kind and helpful

Reception is kind and helpful , everything quick and simple , best urgent care

Yusuf Narmuradov - 11/19/2024


(800) 221-4291