DOT Drug Testing - Fleming Island, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 34 testing centers in the Fleming Island area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

34 DOT Drug-Testing Centers in Fleming Island

Minutes Away, Test Today

Onsite Only

0.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Onsite Only
Fleming Island, FL 32003

1680 Eagle Harbor Parkway, Suite B

0.52 mi
🕑 Testing Hours: Mon–Fri: 12:00 PM-2:00 PM • Sat: 8:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
1680 Eagle Harbor Parkway, Suite B
Orange Park, FL 32003

1465 Kingsley Ave Ste 1401,

4.70 mi
🕑 Testing Hours: Mon–Fri: 12:00 PM-2:00 PM✓ Walk-in with Electronic Donor Pass
1465 Kingsley Ave Ste 1401,
Orange Park, FL 32073

1563 Kingsley Ave Ste 102

4.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1563 Kingsley Ave Ste 102
Orange Park, FL 32073

2140 Kingsley Ave

4.85 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
2140 Kingsley Ave
Orange Park, FL 32073

2020 Kingsley Ave Ste A

4.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2020 Kingsley Ave Ste A
Orange Park, FL 32073

1895 Kingsley Ave Ste 803

4.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1895 Kingsley Ave Ste 803
Orange Park, FL 32073

12303 San Jose Blvd

5.66 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
12303 San Jose Blvd
Jacksonville, FL 32223

3185 Highway 17

6.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3185 Highway 17
Green Cove Springs, FL 32043

1708 Blanding Blvd

7.09 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
1708 Blanding Blvd
Middleburg, FL 32068

705 Ferris Street

7.72 mi
🕑 Testing Hours: M-Th 8:00 AM-5:00 PM • F 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
705 Ferris Street
Green Cove Springs, FL 32043

9826 San Jose Blvd

8.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9826 San Jose Blvd
Jacksonville, FL 32257

9857 Old Saint Augustine Rd Ste 4

8.90 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9857 Old Saint Augustine Rd Ste 4
Jacksonville, FL 32257

4221 Baymeadows Rd Ste 6

10.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4221 Baymeadows Rd Ste 6
Jacksonville, FL 32217

5501 Roosevelt Blvd

10.24 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5501 Roosevelt Blvd
Jacksonville, FL 32244

231 Airline Way

10.38 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
231 Airline Way
Jacksonville, FL 32217

9143 Philips Hwy Ste 495

10.80 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9143 Philips Hwy Ste 495
Jacksonville, FL 32256

7933 Baymeadows Way Ste 3

11.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7933 Baymeadows Way Ste 3
Jacksonville, FL 32256

9770 Old Baymeadows Rd Ste 137

12.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9770 Old Baymeadows Rd Ste 137
Jacksonville, FL 32256

4498 Hendricks Ave

12.58 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
4498 Hendricks Ave
Jacksonville, FL 32207

4237 Salisbury Rd Ste 304

12.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4237 Salisbury Rd Ste 304
Jacksonville, FL 32216

4616 San Juan Ave

12.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4616 San Juan Ave
Jacksonville, FL 32210

1912 Hamilton St Ste 103

12.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1912 Hamilton St Ste 103
Jacksonville, FL 32210

4063 Salisbury Rd Ste 205

12.72 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4063 Salisbury Rd Ste 205
Jacksonville, FL 32216

6639 Southpoint Pkwy Ste 106

12.77 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6639 Southpoint Pkwy Ste 106
Jacksonville, FL 32216

4933-1 University Blvd W

12.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4933-1 University Blvd W
Jacksonville, FL 32216

6671 Hyde Grove Ave

13.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6671 Hyde Grove Ave
Jacksonville, FL 32210

1610 Blanding Blvd

13.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1610 Blanding Blvd
Jacksonville, FL 32210

1584 Normandy Village Parkway Ste 32

13.80 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1584 Normandy Village Parkway Ste 32
Jacksonville, FL 32221

7963 Normandy Blvd

13.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7963 Normandy Blvd
Jacksonville, FL 32221

8705 Perimeter Park Blvd Ste 2

14.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8705 Perimeter Park Blvd Ste 2
Jacksonville, FL 32216

3901 University Blvd S Ste 203

14.31 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3901 University Blvd S Ste 203
Jacksonville, FL 32216

5964 Normandy Blvd

14.48 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
5964 Normandy Blvd
Jacksonville, FL 32205

4372 Southside Blvd, Ste 304

14.61 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4372 Southside Blvd, Ste 304
Jacksonville, FL 32216

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 34 DOT drug testing locations to serve you in and around Fleming Island, FL.

Our Fleming Island, FL testing sites and surrounding areas offer DOT Drug and Alcohol Testing, Physicals, Random pool enrollment, MIS, MVR, and driver qualification reports. We serve Employers, Single operators, and all DOT modes adhering to 49 CFR Part 40 including FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Most locations are minutes away, providing same-day service.

Choose your DOT service or test(s) and find a nearby center with ease—whether for yourself, employees, or others. Our simple process ensures easy scheduling of DOT drug testing near Fleming Island.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Fleming Island, Florida DOT Services

DOT Drug Testing & Services Available in Fleming Island, FL:

Accredited Drug Testing offers comprehensive DOT services in Fleming Island, FL, including drug and alcohol screenings and compliance support, ensuring businesses in Fleming Island, Florida meet federal safety standards.

DOT Services in Fleming Island, FL

DOT regulations ensure safe transportation operations across industries such as FMCSA, FAA, and PHMSA. In Fleming Island, FL, employees must undergo regular drug and alcohol screenings, promoting workplace safety.

At Accredited Drug Testing in Fleming Island, Florida, compliance with DOT regulations is key. Employers must enroll operators in testing programs, offering random, post-accident, and reasonable suspicion testing.

The goal of DOT screening is to maintain a safe and healthy working environment. By adhering to DOT regulations, companies across Fleming Island, Florida enhance their safety protocols and reduce accident risks.

Accredited Drug Testing aids employers in Fleming Island, FL, with DOT-compliant drug and alcohol testing services, helping businesses maintain federal regulation standards.

FMCSA Information

In Fleming Island, FL, FMCSA rules mandate regular drug and alcohol testing for all commercial drivers to ensure roadway safety, with Accredited Drug Testing simplifying compliance processes.

FMCSA regulations in Fleming Island, Florida, require pre-employment, random, and post-accident testing to maintain a workforce free from drug and alcohol abuse.

USCG Information

Accredited Drug Testing supports USCG compliance in Fleming Island, FL, through comprehensive drug and alcohol testing for maritime workers.

USCG regulations in Fleming Island, Florida require rigorous testing to maintain safety and security in maritime operations and employee conduct.

FAA Information

FAA regulations in Fleming Island, FL require stringent drug and alcohol testing for aviation employees, ensuring optimal safety and performance in the skies.

In Fleming Island, Florida, FAA compliance means that aviation employers must conduct regular tests to prevent substance abuse within safety-sensitive functions.

FRA Information

Accredited Drug Testing in Fleming Island, FL facilitates FRA compliance by offering reliable testing protocols for railway workers, maintaining safety in the rail industry.

FRA regulations in Fleming Island, Florida dictate drug and alcohol testing frequency to safeguard rail operations and ensure employee safety.

FTA Information

In Fleming Island, FL, FTA rules enforce regular testing for public transit employees, promoting a secure and efficient transportation network.

FTA compliance in Fleming Island, Florida involves strict adherence to drug and alcohol testing to ensure passenger safety within public transit systems.

PHMSA Information

Accredited Drug Testing helps ensure PHMSA compliance in Fleming Island, FL, through strict testing protocols to safeguard the transportation of hazardous materials.

In Fleming Island, Florida, PHMSA regulations involve routine testing for employees handling hazardous substances, fostering safety and regulatory adherence.

Scheduling in Fleming Island, FL

To arrange any DOT drug or alcohol test in Fleming Island, FL, contact our scheduling team at (800) 221-4291 or utilize our online system by selecting your test and completing the Donor Information section.

Entering your zip code helps find the nearest DOT center in Fleming Island, FL. You'll receive a donor pass with local center details. Walk-ins are welcome, but registration and payment must be completed beforehand.

Our laboratories are SAMHSA-certified, and test results are verified by in-house licensed physicians serving as Medical Review Officers (MRO).

Beyond the Fleming Island location, Accredited Drug Testing offers multiple centers in nearby cities and the metro area.

With over centers nationwide, Accredited Drug Testing facilitates testing on the road or while vacationing, anytime and anywhere!

Florida Dept. of Transportation Updates

Clay County, FL DUI Statistics

Driving under the influence (DUI) continues to be a significant concern in Clay County, where Fleming Island is located. According to the Florida Department of Transportation, Clay County reflects a concerning trend in DUI-related incidents compared to other counties across the state of Florida. These statistics highlight the need for increased awareness and preventive measures to tackle drunk driving. Law enforcement agencies are doubling down on their efforts to reduce DUI cases, emphasizing the impact of intoxication on public safety. Community initiatives aim to educate residents about the legal and personal consequences of driving while impaired in Florida (FL). Efforts to reduce these numbers include public education campaigns, roadside checkpoints, and collaboration with local businesses to promote safer alternatives to driving under the influence. Furthermore, FL lawmakers are considering stricter penalties for repeat offenders as part of a comprehensive approach to combat DUI incidences.

Drug-Involved Accidents in Clay County, FL

Clay County, home to Fleming Island, FL, has seen a worrying trend in drug-involved accidents, aligning with statewide increases observed by the Florida Department of Transportation (FDOT). These incidents include a range of substances, underscoring an urgent need for community and policy interventions. Analysis of recent crash data indicates an uptick in accidents where impairment by substances beyond alcohol contributed to accidents. The county is focused on reducing these statistics by prioritizing drug education programs, with schools and local organizations actively participating in disseminating critical information about the dangers of driving under the influence of drugs. Enhanced law enforcement efforts and advanced drug detection techniques in roadside checks are being employed to mitigate this growing issue. Moreover, public health initiatives are raising awareness about the risks associated with drug-impaired driving as part of a broader Florida state effort to enhance roadway safety.

Marijuana-Related Accidents in Clay County, FL

In the context of Clay County, where Fleming Island, FL is situated, marijuana-related accidents are an emerging concern for the Florida Department of Transportation. With the evolving legal landscape around cannabis, there's been a noticeable rise in accidents where marijuana use was a factor. Although the legalization of medical marijuana in Florida has provided therapeutic benefits to many, it has also posed public safety challenges. Clay County is responding by heightening the emphasis on driver education and awareness regarding the effects of cannabis on driving abilities. Law enforcement and public safety officials are actively developing strategies to address marijuana impairment, given its distinct chemical effects compared to alcohol. The state of FL is investing in research and training for law enforcement officers to better detect and manage marijuana-impaired drivers, ensuring that the roads remain safe for all. Through comprehensive public policy initiatives and community outreach, the county aims to reverse this concerning trend.

Fleming Island Drug Testing

Quickly find trusted local drug testing centers in Fleming Island, FL — fast, convenient, and reliable every time!

Fleming Island DOT Drug Testing

Quickly find a local DOT drug testing center in Fleming Island, FL — fast, reliable, convenient nationwide service!

Fleming Island DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Fleming Island, FL.

Fleming Island Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Fleming Island, FL.v

Fleming Island Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Fleming Island, FL locations—results you can trust, every time!

Fleming Island Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Fleming Island, FL.

Fleming Island Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Fleming Island, FL.

Fleming Island 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Fleming Island, FL employers and individuals nationwide.

Fleming Island 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Fleming Island, FL workplace and personal testing needs.

Fleming Island On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Fleming Island, FL workplace compliant.

Fleming Island DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Fleming Island, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

A very nice company

A very nice company of expertise. The staff will go above to help you out of your situation in a timely matter 👏

Tam Jack - 2/19/2025

Fast and easy

It was fast and easy a pleasure to talk to the customer service people great service

Peter Villegas - 1/19/2025

Pretty Straightforward

It was a pretty straightforward process getting an appointment and making payment. Getting to the testing site was quick. I was in and out in no time.

Sadiat Ogunyemi - 12/19/2024

Kind and very helpful

Called around for hours and posted from company to person and got nowhere and had to fight to get a refund die to there miss information, well save time and sanity Acredited is the ONLY place the person was kind and very helpful, said what I needed and Faith the lady I dealt with made it simple and she did above and beyond everything I needed, as I'm going through a devorse this was a great help and made my day 100 stars from me.

Steven Harrison - 12/19/2024

Excellant Performance

The visit here is always the best . The place is always really clean. The employees are super courteous, very polite, and professional. This is the only drug lab I like to go do my drug and alcohol test. I would like to tell them thank you so much for thier excellent performance and job

Eli Gonzalez - 1/4/2025

Great service

Great service easy use quick processing! Need a hair follicle for my DCFS case , quick and convenient!

Jesse Dupre - 4/12/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

Super helpful and courteous

I've had to use this service twice for out of state physicians we've hired and both times it was super easy. Both customer service reps I spoke with were super helpful and courteous. I won't hesitate to use their service again if needed.

Alicia Rau - 6/19/2024

Very professional and very quick

Very professional and very quick service to schedule appointments! I am pleased with the services and would definitely recommend using them for any drug testing needs!

Carrie Feerick - 4/19/2025

ADT is truly the best

Where do I start?!! ADT is truly the best!! I had the pleasure of speaking with Tori today! She was excellent, professional and truly went above and beyond in her kind, compassionate care and commitment to providing me with the best service possible. I was in such a time crunch, but I was able to call, order the test, pay for it over the phone and get the test I needed within the hour. Not only did this put my mind at ease, they saved my job!! I am forever grateful for this team of hard workers, that care, with such great work ethics! Thank you from the very bottom of my heart!!! If you need a drug, alcohol, etc… screening, this team will be your lifesavers!!

Sarah Patterson - 4/8/2025


(800) 221-4291