DOT Drug Testing - Gainesville, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 34 testing centers in the Gainesville area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

34 DOT Drug-Testing Centers in Gainesville

Minutes Away, Test Today

720 Sw 2nd Ave Ste 160a

0.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
720 Sw 2nd Ave Ste 160a
Gainesville, FL 32601

825 Northwest 23rd Ave., Unit 2

1.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
825 Northwest 23rd Ave., Unit 2
Gainesville, FL 32609

4881 Nw 8th Ave Ste 2a

4.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4881 Nw 8th Ave Ste 2a
Gainesville, FL 32605

4343 W Newberry Rd Ste 10

4.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4343 W Newberry Rd Ste 10
Gainesville, FL 32607

3925 Nw 43rd St

4.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3925 Nw 43rd St
Gainesville, FL 32606

812 Nw 57th St

4.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
812 Nw 57th St
Gainesville, FL 32605

6121 Nw 1st Pl

4.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6121 Nw 1st Pl
Gainesville, FL 32607

1131 Nw 64th Ter Ste A

5.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1131 Nw 64th Ter Ste A
Gainesville, FL 32605

3581 Sw Archer Rd Units 30 And 40

6.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3581 Sw Archer Rd Units 30 And 40
Gainesville, FL 32608

14819 Nw 140th St

13.96 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14819 Nw 140th St
Alachua, FL 32615

14417 Nw 152nd Ln

14.61 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14417 Nw 152nd Ln
Alachua, FL 32615

420 Ne Santa Fe Blvd

20.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
420 Ne Santa Fe Blvd
High Springs, FL 32643

1550 S Water St

22.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1550 S Water St
Starke, FL 32091

345 W Madison St

23.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
345 W Madison St
Starke, FL 32091

850 E Main St Attn Lab

26.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
850 E Main St Attn Lab
Lake Butler, FL 32054

128 Nw 137th Dr

30.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
128 Nw 137th Dr
Newberry, FL 32669

1113 Nw 23rd Ave

34.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1113 Nw 23rd Ave
Chiefland, FL 32626

1109 Sw 10th St

34.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1109 Sw 10th St
Ocala, FL 34471

3470 Nw 57th Trl

34.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3470 Nw 57th Trl
Bell, FL 32619

31 Sw 12th St

34.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
31 Sw 12th St
Ocala, FL 34471

1834 Sw 1st Ave Ste 201

35.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1834 Sw 1st Ave Ste 201
Ocala, FL 34471

2415 Sw College Rd

35.18 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
2415 Sw College Rd
Ocala, FL 34471

1015 Se 17th St Ste 200

35.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1015 Se 17th St Ste 200
Ocala, FL 34471

2685 Sw 32nd Place,

35.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2685 Sw 32nd Place,
Ocala, FL 34471

2100 Se 17th St Ste 902

35.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2100 Se 17th St Ste 902
Ocala, FL 34471

8602 Sw State Road 200, 103rd Street Plaza, Suite J

36.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8602 Sw State Road 200, 103rd Street Plaza, Suite J
Ocala, FL 34481

6160 Sw Highway 200

37.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6160 Sw Highway 200
Ocala, FL 34476

205 Zeagler Dr, Suite 201

37.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
205 Zeagler Dr, Suite 201
Palatka, FL 32177

6500 Crill Ave

38.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6500 Crill Ave
Palatka, FL 32177

6101 Crill Ave

38.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6101 Crill Ave
Palatka, FL 32177

6100 Saint Johns Ave Ste C

38.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6100 Saint Johns Ave Ste C
Palatka, FL 32177

3824 E Us Highway 90

40.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3824 E Us Highway 90
Lake City, FL 32055

3824 W Us Highway 90

40.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3824 W Us Highway 90
Lake City, FL 32055

265 Sw Malone Street,suite 105

40.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
265 Sw Malone Street,suite 105
Lake City, FL 32025

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 34 DOT drug testing locations to serve you in and around Gainesville, FL.

In Gainesville, FL, and areas nearby, our testing facilities specialize in DOT drug testing, alcohol tests, physicals, and additional services such as random pool enrollment and driver reports. We cater to businesses, individual operators, and all DOT modes under 49 CFR Part 40, including FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Conveniently located, our centers offer same-day services in most situations, making it hassle-free to meet DOT requirements.

Choose the desired DOT service or drug/alcohol test for yourself, employees, or others and locate a center near you. Our user-friendly process simplifies scheduling DOT drug testing in Gainesville, ensuring seamless compliance.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Gainesville, Florida DOT Services

DOT Drug Testing & Services Available in Gainesville, FL:

Accredited Drug Testing in Gainesville, FL, offers comprehensive DOT solutions including drug and alcohol screenings, ensuring companies meet all federal compliance standards with convenience.

DOT Services in Gainesville, FL

DOT regulations are stringent on drug and alcohol screenings to ensure safety in workplaces, particularly in Gainesville, FL. They mandate regular testing and maintain high safety standards across all occupations.

In Gainesville, DOT regulations necessitate routine drug tests to enhance workplace safety and ensure employees are sober and ready for duty at all times.

To comply with DOT's strict standards, businesses in Gainesville, FL, must conduct random alcohol screenings and drug tests for employees, fostering a safe working environment.

Gainesville's employment sectors adhere to DOT guidelines, emphasizing frequent drug testing for enhanced safety, which is paramount in ensuring employee health and job readiness.

FMCSA Information

In Gainesville, FL, FMCSA rules ensure commercial vehicle operators undergo drug and alcohol tests, safeguarding road safety by maintaining sobriety standards.

Gainesville, Florida prioritizes FMCSA testing, reinforcing operator compliance with regulations to ensure secure transportation networks.

USCG Information

USCG standards require drug and alcohol screenings in Gainesville, FL for maritime personnel, ensuring safe and compliant offshore operations.

Sailors in Gainesville, Florida are subject to USCG testing, maintaining safety protocols for maritime activities.

FAA Information

FAA guidelines in Gainesville, FL, demand drug and alcohol tests for aviation personnel to ensure high safety standards in air travel operations.

Airlines in Gainesville, Florida follow FAA regulations, conducting frequent tests to certify sober and efficient aviation practices.

FRA Information

Railway workers in Gainesville, FL, undergo FRA-mandated testing to ensure safe and effective railroad operations, adhering to strict DOT guidelines.

FRA rules in Gainesville foster a culture of safety, with regular testing protocols for rail industry employees.

FTA Information

FTA regulations in Gainesville, FL, enforce drug testing for transit workers, ensuring public transport safety and operator readiness.

Gainesville's transit agencies comply with FTA standards, conducting tests to uphold passenger safety and service quality.

PHMSA Information

PHMSA guidelines enforce strict testing in Gainesville, FL for employees handling hazardous materials, ensuring safety in transport activities.

Adhering to PHMSA directives in Gainesville, Florida, businesses uphold high safety measures via regular drug testing.

Scheduling in Gainesville, FL

For scheduling any DOT drug or alcohol test in Gainesville, FL, reach out to our local team at (800) 221-4291 or use our online express registration. Register first to visit the testing center.

Enter your zip code to locate the nearest testing center in Gainesville, Florida, and receive your donor pass via email. Bring this document along, although no appointment is often required. Complete the donor section and payment during registration.

Our labs uphold SAMHSA certifications, ensuring all test outcomes are reviewed by our licensed Medical Review Officers (MROs).

Besides Gainesville, FL, Accredited Drug Testing operates in multiple nearby cities, broadening our reach in the metropolitan region.

Wherever your travels take you, Accredited Drug Testing provides nationwide coverage, allowing test scheduling at any location.

Florida Dept. of Transportation Updates

DUI Statistics in Gainesville, FL

Gainesville, located in Alachua County, Florida, experiences its share of DUI (Driving Under the Influence) incidents each year. The state's stringent DUI laws aim to mitigate these occurrences by imposing severe penalties including fines, license suspensions, and potential jail time. Gainesville, with its youthful population largely attributed to the University of Florida, sees a notable number of DUI arrests, especially during festive seasons and holidays. The State of Florida reports that DUI-related accidents contribute significantly to traffic fatalities, urging local authorities and the Florida Department of Transportation (FDOT) to promote awareness campaigns and implement measures to reduce DUI incidences. Collaborative efforts between law enforcement and community organizations in Gainesville, FL, focus on education and prevention, aiming to decrease these statistics over time. DUI checkpoints and increased patrol efforts are regularly employed across the county to identify and deter impaired drivers, making road safety a high priority in Florida.

Drug Involved Accidents in Gainesville, FL

Gainesville, FL, within Alachua County, witnesses a substantial number of drug involved accidents annually. These incidents serve as a critical concern for the Florida Department of Transportation (FDOT) and local law enforcement, who work cohesively to address this persistent issue. The misuse of prescription medications and the prevalence of illegal substances contribute to impaired driving incidents, emphasizing the need for robust educational and logistical strategies in Florida. Statistics indicate a diverse demographic involved in such accidents, thus necessitating tailored interventions across the Gainesville community. Local initiatives, backed by the State of Florida, strive to improve public understanding and reduce these occurrences through seminars, school programs, and the promotion of access to resources for those struggling with substance abuse. Alachua County's proactive approach includes collaborations with healthcare providers to identify and support at-risk individuals, aiming for long-term reductions in drug-involved vehicular incidents in Gainesville.

Marijuana Related Accidents in Gainesville, FL

In Gainesville, Florida, the rise of marijuana use has subtly influenced traffic safety dynamics, with a measurable impact on marijuana-related accidents within Alachua County. As laws around marijuana evolve in Florida, monitoring its effect on driver impairment and traffic incidents becomes imperative. Gainesville, being an educational and cultural hub, reflects a complex interplay between legalization debates and public health. Studies in Florida underscore that while alcohol remains predominant in impaired driving statistics, cannabis-related accidents are increasingly reported, necessitating strategic responses from the FDOT and community stakeholders. Public awareness campaigns specific to Gainesville, FL, encourage responsible consumption and highlight the risks associated with impaired driving. The Gainesville Police Department collaborates with state agencies to employ driving simulations and outreach programs to educate residents on the legal repercussions and safety hazards of driving under the influence of marijuana, striving to adjust perceptions and reduce related incidents in Florida.

Gainesville Drug Testing

Quickly find trusted local drug testing centers in Gainesville, FL — fast, convenient, and reliable every time!

Gainesville DOT Drug Testing

Quickly find a local DOT drug testing center in Gainesville, FL — fast, reliable, convenient nationwide service!

Gainesville DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Gainesville, FL.

Gainesville Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Gainesville, FL.v

Gainesville Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Gainesville, FL locations—results you can trust, every time!

Gainesville Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Gainesville, FL.

Gainesville Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Gainesville, FL.

Gainesville 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Gainesville, FL employers and individuals nationwide.

Gainesville 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Gainesville, FL workplace and personal testing needs.

Gainesville On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Gainesville, FL workplace compliant.

Gainesville DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Gainesville, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

Super Helpful

Detricia was super helpful in making sure that the drug test is sent my worker for was the right one I needed for my company to be compliant with my customers drug test requirements.

Kevin Howell - 12/19/2024

Very professional and very quick

Very professional and very quick service to schedule appointments! I am pleased with the services and would definitely recommend using them for any drug testing needs!

Carrie Feerick - 4/19/2025

Quick, knowledgeable and courteous

Quick, knowledgeable and courteous Janelle worked diligently to support me. I am recommending their services to anyone looking.

Greensboro Joseph - 11/19/2024


(800) 221-4291