DOT Drug Testing - Hernando, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 34 testing centers in the Hernando area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

34 DOT Drug-Testing Centers in Hernando

Minutes Away, Test Today

3041 Landover Blvd.,

1.30 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-2:00 PM • Sat: 6:00 AM-10:00 AM✓ Walk-in with Electronic Donor Pass
3041 Landover Blvd.,
Spring Hill, FL 34608

4112 Mariner Blvd

1.39 mi
🕑 Testing Hours: Mon–Sat: 9:00 AM-9:00 PM • Sun: 10:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
4112 Mariner Blvd
Spring Hill, FL 34609

3037 Landover Blvd

1.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3037 Landover Blvd
Spring Hill, FL 34608

40 Seven Hills Dr,

2.97 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
40 Seven Hills Dr,
Spring Hill, FL 34609

10441 Quality Dr Ste 105

3.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10441 Quality Dr Ste 105
Spring Hill, FL 34609

11123 County Line Rd

3.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11123 County Line Rd
Spring Hill, FL 34609

170 Mariner Blvd., Unit 7-a

3.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
170 Mariner Blvd., Unit 7-a
Spring Hill, FL 34609

12114 Cortez Blvd #205

3.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12114 Cortez Blvd #205
Brooksville, FL 34613

12120 Cortez Blvd

3.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12120 Cortez Blvd
Brooksville, FL 34613

12220 Cortez Blvd

3.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12220 Cortez Blvd
Brooksville, FL 34613

7007 Nightwalker Rd,

3.93 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-3:00 PM • Sat: 6:30 AM-10:30 AM✓ Walk-in with Electronic Donor Pass
7007 Nightwalker Rd,
Brooksville, FL 34613

17240 Cortez Blvd

6.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
17240 Cortez Blvd
Brooksville, FL 34601

20205 Cortez Blvd

8.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
20205 Cortez Blvd
Brooksville, FL 34601

14000 Fivay Rd Injury Care Only

10.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14000 Fivay Rd Injury Care Only
Hudson, FL 34667

14100 Fivay Rd Ste 140

12.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14100 Fivay Rd Ste 140
Hudson, FL 34667

13944 Lakeshore Blvd Ste D

12.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
13944 Lakeshore Blvd Ste D
Hudson, FL 34667

7459 State Road 52,

14.16 mi
🕑 Testing Hours: Mon–Fri: 5:30 AM-2:00 PM • Sat: 6:00 AM-10:30 AM✓ Walk-in with Electronic Donor Pass
7459 State Road 52,
Hudson, FL 34667

11528 Us Highway 19

14.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11528 Us Highway 19
Port Richey, FL 34668

9328 Us Highway 19

16.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9328 Us Highway 19
Port Richey, FL 34668

31075 Cortez Blvd

18.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
31075 Cortez Blvd
Brooksville, FL 34602

5504 Gateway Blvd

19.80 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5504 Gateway Blvd
Wesley Chapel, FL 33544

27421 State Road 54

20.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
27421 State Road 54
Wesley Chapel, FL 33544

5400 School Rd

20.38 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5400 School Rd
New Port Richey, FL 34652

10730 State Road 54

20.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10730 State Road 54
Trinity, FL 34655

8745 State Road 54

20.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8745 State Road 54
New Port Richey, FL 34655

5124 Us Highway 19

20.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5124 Us Highway 19
New Port Richey, FL 34652

22945 State Road 54

20.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
22945 State Road 54
Lutz, FL 33549

4741 Us Highway 19,

21.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4741 Us Highway 19,
New Port Richey, FL 34652

24420 State Road 54

21.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
24420 State Road 54
Lutz, FL 33559

13933 17th St Ste 101

21.35 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
13933 17th St Ste 101
Dade City, FL 33525

34498 Cortez Blvd

21.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
34498 Cortez Blvd
Ridge Manor, FL 33523

26827 Foggy Creek Rd, Bldg. 6 Suite 102

21.94 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-2:00 PM • Sat: 7:30 AM-11:30 AM✓ Walk-in with Electronic Donor Pass
26827 Foggy Creek Rd, Bldg. 6 Suite 102
Wesley Chapel, FL 33544

3956 S Suncoast Blvd

22.84 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
3956 S Suncoast Blvd
Homosassa, FL 34448

1724 Bruce B Downs Blvd Ste Q2

22.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1724 Bruce B Downs Blvd Ste Q2
Wesley Chapel, FL 33544

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 34 DOT drug testing locations to serve you in and around Hernando, FL.

Our local Hernando, FL DOT drug testing centers offer comprehensive DOT services including drug and alcohol testing, physicals, and compliance for FMCSA, FTA, FAA, USCG, FRA, and PHMSA under 49 CFR Part 40. Conveniently located, we facilitate same-day appointments.

Select your DOT service or test, then choose a nearby center. Our efficient process enables easy arrangement of testing for individuals or employees near Hernando.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Hernando, Florida DOT Services

DOT Drug Testing & Services Available in Hernando, FL:

In Hernando, FL, Accredited Drug Testing offers a wide range of DOT services to facilitate compliance and safety for employers and employees, ensuring a streamlined testing process.

DOT Services in Hernando, FL

DOT testing regulations are crucial for maintaining safety standards across workplaces in Hernando, Florida. Employers must adhere to these stringent procedures, ensuring a safe work environment for all.

Compliance with DOT drug and alcohol testing is essential in Hernando, FL, with procedures designed to detect substance abuse promptly, promoting overall occupational safety.

In Hernando, DOT regulations require routine drug examinations for transportation workers, maintaining high safety levels and ensuring safe transit operations.

Regular training in Hernando on DOT protocols ensures personnel understand and comply with the necessary safety and testing requirements to maintain an addiction-free workplace.

FMCSA Information

In Hernando, FL, FMCSA mandates strict drug and alcohol testing for drivers, promoting public safety on highways. Complying with these rules ensures accident reduction.

The FMCSA testing regulations in Hernando, Florida, emphasize safety in commercial driving by enforcing strict substance testing protocols.

USCG Information

USCG applies strict testing rules in Hernando, FL, ensuring maritime safety by regulating substance use among crew members on commercial vessels.

Substance controls under USCG in Hernando, Florida, ensure maritime operators maintain focused and reliable operation in the water bodies.

FAA Information

The FAA in Hernando, FL, enforces drug and alcohol testing for aviation personnel, ensuring safety in air travel and preventing substance-related hazards.

FAA regulations in Hernando, Florida, ensure aviation workers are substance-free to uphold the safety and reliability of air travel processes.

FRA Information

Under FRA guidelines in Hernando, FL, railway workers undergo regular testing to prevent substance influence on operations, ensuring rail safety.

FRA imposes drug and alcohol tests in Hernando, Florida, for rail safety, crucial for safe train operation and passenger security.

FTA Information

FTA promotes safety in Hernando, FL, through required testing for transit employees, helping in maintaining safe public transportation systems free of drug influence.

FTA regulations in Hernando, Florida, are vital for transit system safety, mandating drug tests to prevent impairment-related incidents.

PHMSA Information

PHMSA regulations in Hernando, FL, require mandatory drug testing for pipeline operators, ensuring safety in operations handling hazardous materials.

In Hernando, Florida, PHMSA testing plays a critical role in maintaining operational safety standards for public health and environment protection.

Scheduling in Hernando, FL

For DOT drug or alcohol testing in Hernando, FL, call us at (800) 221-4291 or register online using our express system. Complete the registration before visiting the testing center.

Enter your zip code to locate the nearest testing facility in Hernando, Florida. A registration form with all details will be emailed to you. No appointment necessary, but complete the donor info and payment upfront.

Our labs bear SAMHSA certification, with results verified by our Medical Review Officers (MRO) for reliability.

Accredited Drug Testing operates additional centers in Hernando's surrounding cities, enhancing service availability.

Nationwide, whether traveling or on vacation, our expansive testing network caters to your needs anytime, anywhere.

Florida Dept. of Transportation Updates

DUI Statistics in Hernando County, FL

Hernando County, located in central Florida, has been experiencing an increase in DUI-related incidents over recent years. These DUI statistics are a crucial focus for the Florida Department of Transportation (FDOT) as the state's road safety measures continue to adapt to the rising number of incidents involving impaired driving. In Hernando County specifically, DUI arrests have shown variability but generally trend upward, reflecting broader state-wide patterns. Florida's law enforcement agencies, including those in Hernando County, actively engage in DUI checkpoints and initiatives to combat impaired driving. Programs aimed at raising awareness about the dangers of driving under the influence are also prevalent, aligning with Florida's overarching goal of reducing DUI-related accidents and fatalities to improve road safety.

Drug-Involved Accidents in Hernando County, Florida

Hernando County, FL, mirrors trends seen across the state concerning drug-involved accidents. The Florida Department of Transportation (FDOT) has noted that accidents involving drugs other than alcohol, including both prescription and illicit substances, contribute significantly to traffic incidents in the county. Educational programs and law enforcement operations addressing the impact of drugs on driving ability are part of Florida's efforts to mitigate these types of accidents. Statistics indicate that the use of substances like opioids, benzodiazepines, and illegal drugs contributes to impaired driving conditions, and Hernando County is no exception. This focus aims to enhance public safety and reduce the number of drug-related incidents on roads in Hernando County, a key concern for the FDOT and local law enforcement.

Marijuana-Related Accidents in Hernando County, FL

In Hernando County, Florida, marijuana-related traffic accidents have been a growing area of concern, reflective of statewide trends where the legalization and decriminalization of marijuana have influenced driving behaviors. Florida's Department of Transportation (FDOT) is closely monitoring the effect of marijuana on driving safety. Hernando County's law enforcement agencies are adapting strategies to address the challenges posed by marijuana impairment, which can include slower reaction times and impaired judgment critical for driving safely. Florida has increased efforts in both education and enforcement, aiming to address marijuana's impact on road safety. These initiatives help ensure that motorists in Hernando County, FL, are aware of the risks associated with using marijuana before driving, thus contributing to reducing road accidents related to drug impairment.

Hernando Drug Testing

Quickly find trusted local drug testing centers in Hernando, FL — fast, convenient, and reliable every time!

Hernando DOT Drug Testing

Quickly find a local DOT drug testing center in Hernando, FL — fast, reliable, convenient nationwide service!

Hernando DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Hernando, FL.

Hernando Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Hernando, FL.v

Hernando Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Hernando, FL locations—results you can trust, every time!

Hernando Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Hernando, FL.

Hernando Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Hernando, FL.

Hernando 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Hernando, FL employers and individuals nationwide.

Hernando 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Hernando, FL workplace and personal testing needs.

Hernando On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Hernando, FL workplace compliant.

Hernando DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Hernando, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

Great service

Great service easy use quick processing! Need a hair follicle for my DCFS case , quick and convenient!

Jesse Dupre - 4/12/2025

A very nice company

A very nice company of expertise. The staff will go above to help you out of your situation in a timely matter 👏

Tam Jack - 2/19/2025

Extremely pleasant

Initially Torrie helped me but I needed to call back. When I called back Kayla stepped in and finished my order. Both were extremely pleasant over the phone and provided wonderful customer service. Kudos to them!

Brynne Beverly - 4/19/2024

I love the customer service

I love the customer service. The lady was patient enough to help me book my appointment with ease and ensuring I get the location that's best for me. Keep up the good work!

Elizabeth Chiaka - 9/19/2024

This is by far the easiest

This is by far the easiest way to get my lab work ordered and paid for. The phone calls are short and to the point. They don’t try to push extra sales on you and when I walk in to the clinic I simply show my donor pass and with in a matter of minutes I’m done. I will continue to use ADT in the future.

Jason Jackson - 7/19/2025

Saved My CDL!

Time was running out before my Cdl got downgraded because of a violation I had on clearinghouse. I couldn't find an employer to send me for my return to duty test, but these guys had my test scheduled and done in the same day! They saved my cdl. Thank you again!

Michael Williams - 12/2/2024

Sweet and helpful

Trish was amazing and got me through the sytem very fast and swift. I had a hard time hearing her a couple of times, but she was super sweet and helpful throughout the process. Highly recommend her!

Sophia Schutze - 6/19/2024

Smooth and Seamless

I use their service for new hire and DOT employee's. Spoke with Taisha Walker this morning, and she was very helpful. She made the process smooth and seamless.

Christina Galdos - 3/9/2025

Clear and Competent

I was so surprised with the quality of customer service I received today when I called Accredited Drug Testing. The representative was so clear and competent. She was able to reschedule an appointment at my preferred location. The representative whose name is Justice, was so polite and competent. She expedited the whole process. It was a relief to me.

Rachel Padian - 9/6/2024

Thank You!!

I needed a quick drug test for visitation with my children. I called at 6am they answered and set me up with a place a couple miles from my house to go get the test done, that was on Wednesday, I had the results on Friday afternoon and was able to see my kids on Saturday. Thank you for the help!

Mark Broadhead - 2/12/2025


(800) 221-4291