DOT Drug Testing - Oviedo, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 38 testing centers in the Oviedo area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

38 DOT Drug-Testing Centers in Oviedo

Minutes Away, Test Today

1410 W Broadway St Ste 108

1.05 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1410 W Broadway St Ste 108
Oviedo, FL 32765

1950 W Sr 426 Ste 108,

1.68 mi
🕑 Testing Hours: Mon–Fri: 11:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
1950 W Sr 426 Ste 108,
Oviedo, FL 32765

8010 Red Bug

1.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8010 Red Bug
Oviedo, FL 32765

5355 Red Bug Lake Rd

3.17 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
5355 Red Bug Lake Rd
Winter Springs, FL 32708

5931 Brick Court Suite 100

5.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5931 Brick Court Suite 100
Winter Park, FL 32792

7457 Aloma Ave Ste 301

6.31 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7457 Aloma Ave Ste 301
Winter Park, FL 32792

7480 Aloma Ave

6.35 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7480 Aloma Ave
Winter Park, FL 32792

2200 N Alafaya Trl Ste 900

6.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2200 N Alafaya Trl Ste 900
Orlando, FL 32826

2000 N Alafaya Trail, Suite 100

6.71 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-2:00 PM • Sat: 7:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
2000 N Alafaya Trail, Suite 100
Orlando, FL 32826

4122 Metric Dr Ste 800

7.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4122 Metric Dr Ste 800
Winter Park, FL 32792

11250 E Colonial Dr

7.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11250 E Colonial Dr
Orlando, FL 32817

5931 Aloma Ave Ste 100

7.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5931 Aloma Ave Ste 100
Winter Park, FL 32792

11867 E Colonial Dr

7.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11867 E Colonial Dr
Orlando, FL 32826

1239 Sr 436 Walmart #101

7.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1239 Sr 436 Walmart #101
Casselberry, FL 32707

3099 Aloma Ave

7.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3099 Aloma Ave
Winter Park, FL 32792

238 Wilshire Blvd Ste 120

7.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
238 Wilshire Blvd Ste 120
Casselberry, FL 32707

250 N Alafaya Trl Ste 135

8.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
250 N Alafaya Trl Ste 135
Orlando, FL 32828

12265 Lake Underhill Rd Ste 135

8.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12265 Lake Underhill Rd Ste 135
Orlando, FL 32825

12301 Lake Underhill Rd Ste 118

8.76 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12301 Lake Underhill Rd Ste 118
Orlando, FL 32828

705 W State Road 434 Ste J & K

8.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
705 W State Road 434 Ste J & K
Longwood, FL 32750

855 S Us Highway 17 92

9.05 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
855 S Us Highway 17 92
Longwood, FL 32750

136 Parliament Loop Ste 1020

9.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
136 Parliament Loop Ste 1020
Lake Mary, FL 32746

1060 West Sr 434, Ste 116

9.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1060 West Sr 434, Ste 116
Longwood, FL 32750

512 E Altamonte Dr Ste 1000

9.72 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
512 E Altamonte Dr Ste 1000
Altamonte Springs, FL 32701

611 N Wymore Rd Ste 220

10.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
611 N Wymore Rd Ste 220
Winter Park, FL 32789

499 E Central Pkwy Ste 245

10.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
499 E Central Pkwy Ste 245
Altamonte Springs, FL 32701

2504 S Alafaya Trl, Ste 160

10.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2504 S Alafaya Trl, Ste 160
Orlando, FL 32828

483 N Semoran Blvd Suite 103

10.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
483 N Semoran Blvd Suite 103
Winter Park, FL 32792

501 N Orlando Ave Ste 227

10.58 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
501 N Orlando Ave Ste 227
Winter Park, FL 32789

36 S Semoran Blvd Ste B

10.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
36 S Semoran Blvd Ste B
Orlando, FL 32807

393 Center Pointe Cir Ste 1457

10.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
393 Center Pointe Cir Ste 1457
Altamonte Springs, FL 32701

270 Northlake Blvd Ste 1004

10.83 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
270 Northlake Blvd Ste 1004
Altamonte Springs, FL 32701

910 Lexington Green Lane

10.85 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM (Lunch: 12:00 PM - 12:30 PM)⚡ Drug Screens: Mon–Fri: 06:30 AM - 02:30 PM
910 Lexington Green Lane
Sanford, FL 32771

1850 Lee Rd Ste 300

10.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1850 Lee Rd Ste 300
Winter Park, FL 32789

1850 Lee Rd Ste 216

10.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1850 Lee Rd Ste 216
Winter Park, FL 32789

691 Douglas Ave

11.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
691 Douglas Ave
Altamonte Springs, FL 32714

509 S Semoran Blvd

11.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
509 S Semoran Blvd
Orlando, FL 32807

901 N Lake Destiny Rd Ste 400

11.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
901 N Lake Destiny Rd Ste 400
Maitland, FL 32751

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 38 DOT drug testing locations to serve you in and around Oviedo, FL.

Our DOT drug testing centers in Oviedo, FL and nearby provide an array of DOT services including drug and alcohol tests, physicals, random pool and Consortium enrollments, MIS and MVR reports, driver qualification, and more, all compliant with 49 CFR Part 40 standards. We serve diverse DOT modes such as FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Conveniently located within minutes from your home or office, we offer same-day service for most cases.

Choose your required DOT service or drug/alcohol test at a nearby center—our process is designed to be seamless and efficient, accommodating individuals, employees, or others in need of testing. Effortlessly plan your DOT drug testing in Oviedo.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Oviedo, Florida DOT Services

DOT Drug Testing & Services Available in Oviedo, FL:

Accredited Drug Testing offers comprehensive DOT services in Oviedo, FL, addressing diverse needs from drug and alcohol screening to driver reports and MIS forms, ensuring regulatory compliance.

DOT Services in Oviedo, FL

DOT regulations ensure safety through mandatory drug and alcohol testing protocols. In Oviedo, FL, these standards safeguard transportation workers, maintaining operational security and public confidence.

Regulations require routine screenings for substances, ensuring all employees comply within Oviedo, Florida. This policy minimizes risks and promotes a secure work environment.

Employers in Oviedo, FL adhere to these mandates, implementing strict testing schedules. This vigilance ensures consistent safety standards across the board.

The DOT guidelines enhance workplace reliability by thoroughly monitoring workers in safety-sensitive positions within Oviedo, Florida, upholding public trust.

FMCSA Information

FMCSA mandates robust drug testing for transport workers in Oviedo, FL, promoting safety. This regulation is pivotal for commercial driving operations within the area.

Regular alcohol tests are administered under FMCSA directives in Oviedo, Florida, ensuring compliance and reducing accident risks in the transport sector.

USCG Information

USCG drug testing in Oviedo, FL safeguards maritime operations, ensuring compliance with federal safety protocols for crew members.

Oviedo, Florida-based USCG enforces alcohol testing, reinforcing discipline and safety on both commercial and recreational vessels.

FAA Information

FAA's drug testing guidelines in Oviedo, FL are critical, maintaining aviation safety standards, ensuring pilots and crew are substance-free.

Alcohol testing helps regulate compliance among aviation professionals in Oviedo, Florida, reinforcing trust and safety in air travel.

FRA Information

FRA enforces drug testing for rail workers in Oviedo, FL, protecting rail safety and ensuring operators work without impairments.

Alcohol screenings under FRA rules in Oviedo, Florida, help maintain public transit integrity, minimizing safety breaches.

FTA Information

FTA drug policies in Oviedo, FL keep transit environments secure, verifying bus and train operators are drug-free, enhancing commuter safety.

Alcohol testing under FTA jurisdiction ensures public transport systems in Oviedo, Florida, operate smoothly and safely.

PHMSA Information

PHMSA enforces stringent drug testing for its personnel in Oviedo, FL, ensuring hazardous material transportation safety and adherence to federal standards.

In Oviedo, Florida, PHMSA's alcohol testing rules sustain high safety levels in pipeline operation sectors, managing risk effectively.

Scheduling in Oviedo, FL

To arrange a DOT drug or alcohol test in Oviedo, FL, contact us at (800) 221-4291 or use our online system. Select your test, fill out the Donor Information, and register before heading to our centers.

Enter your zip code to find the nearest testing center in Oviedo, FL. After registration, a donor pass with center details will be emailed to you. Bring it with you, no appointment needed but ensure you are registered and have paid.

Our labs are SAMHSA-certified, and all results are validated by licensed Medical Review Officers (MROs) on staff.

Besides Oviedo, FL, Accredited Drug Testing offers services in various locations within the metro area.

Wherever you are, Accredited Drug Testing offers nationwide service, scheduling tests anytime, anywhere.

Florida Dept. of Transportation Updates

DUI Statistics in Oviedo, FL and Seminole County

In Oviedo, Florida, and the broader Seminole County, the issue of Driving Under the Influence (DUI) remains a significant concern for the Department of Transportation (DOT) and local authorities. Florida (FL) has strict laws and initiatives aimed at curbing DUI incidents, reflecting a statewide emphasis on road safety. Seminole County, where Oviedo is located, is no exception to this trend. Authorities have implemented various programs to raise awareness and reduce the incidence of impaired driving. Recent data indicates a continued effort by law enforcement to actively monitor and enforce DUI laws, ensuring the safety of residents and visitors. The continuity of campaigns like "Drive Sober or Get Pulled Over" signifies FL’s commitment to combatting DUI, fostering a safer driving environment. Efforts include increased patrols and sobriety checkpoints in areas with higher reported incidents.

Drug-Involved Accidents in Oviedo, FL and Seminole County

Drug-involved accidents are a growing concern in Oviedo, Florida, and its surrounding Seminole County, attracting the attention of the Department of Transportation (DOT). With the nationwide increase in prescription and illicit drug use, the impact on road safety in FL is evident. Seminole County authorities are vigilant in addressing this issue, implementing more stringent traffic monitoring and control measures. They collaborate closely with the Florida Highway Patrol to ensure resources are effectively deployed to combat this trend. Educational initiatives and community outreach programs have been expanded to address the dangers of drug-impaired driving. The FL DOT actively engages in collecting data to analyze patterns, responding with appropriate measures to minimize occurrences. Seminole County has seen enhanced cooperation between law enforcement and public health agencies to tackle the root causes contributing to drug-related crashes.

Marijuana-Related Accidents in Oviedo, FL and Seminole County

As states across the USA, including Florida (FL), navigate changing marijuana laws, the impact on road safety in Oviedo and Seminole County is closely monitored by the Department of Transportation (DOT). Seminole County has experienced an increase in marijuana-related accidents, correlating with a broader national trend following the relaxation of marijuana prohibitions. Local authorities, alongside the FL DOT, have intensified efforts to understand and mitigate these risks. Awareness campaigns about the legal limits and effects of marijuana on driving, such as THC impairment, are part of the county's strategy to address this issue. Law enforcement agencies in Seminole County have enhanced training programs to better identify and respond to marijuana-impaired drivers on the roads. Additionally, the integration of advanced detection technologies aims to improve traffic safety outcomes, ensuring drivers remain aware of the consequences of impaired driving.

Oviedo Drug Testing

Quickly find trusted local drug testing centers in Oviedo, FL — fast, convenient, and reliable every time!

Oviedo DOT Drug Testing

Quickly find a local DOT drug testing center in Oviedo, FL — fast, reliable, convenient nationwide service!

Oviedo DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Oviedo, FL.

Oviedo Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Oviedo, FL.v

Oviedo Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Oviedo, FL locations—results you can trust, every time!

Oviedo Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Oviedo, FL.

Oviedo Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Oviedo, FL.

Oviedo 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Oviedo, FL employers and individuals nationwide.

Oviedo 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Oviedo, FL workplace and personal testing needs.

Oviedo On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Oviedo, FL workplace compliant.

Oviedo DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Oviedo, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291