DOT Drug Testing - Winter Springs, FL

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 37 testing centers in the Winter Springs area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

37 DOT Drug-Testing Centers in Winter Springs

Minutes Away, Test Today

1239 Sr 436 Walmart #101

1.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1239 Sr 436 Walmart #101
Casselberry, FL 32707

855 S Us Highway 17 92

2.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
855 S Us Highway 17 92
Longwood, FL 32750

705 W State Road 434 Ste J & K

2.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
705 W State Road 434 Ste J & K
Longwood, FL 32750

1060 West Sr 434, Ste 116

3.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1060 West Sr 434, Ste 116
Longwood, FL 32750

238 Wilshire Blvd Ste 120

3.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
238 Wilshire Blvd Ste 120
Casselberry, FL 32707

136 Parliament Loop Ste 1020

4.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
136 Parliament Loop Ste 1020
Lake Mary, FL 32746

512 E Altamonte Dr Ste 1000

4.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
512 E Altamonte Dr Ste 1000
Altamonte Springs, FL 32701

499 E Central Pkwy Ste 245

4.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
499 E Central Pkwy Ste 245
Altamonte Springs, FL 32701

5355 Red Bug Lake Rd

4.82 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
5355 Red Bug Lake Rd
Winter Springs, FL 32708

691 Douglas Ave

5.13 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
691 Douglas Ave
Altamonte Springs, FL 32714

393 Center Pointe Cir Ste 1457

5.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
393 Center Pointe Cir Ste 1457
Altamonte Springs, FL 32701

270 Northlake Blvd Ste 1004

5.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
270 Northlake Blvd Ste 1004
Altamonte Springs, FL 32701

1410 W Broadway St Ste 108

5.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1410 W Broadway St Ste 108
Oviedo, FL 32765

1950 W Sr 426 Ste 108,

5.76 mi
🕑 Testing Hours: Mon–Fri: 11:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
1950 W Sr 426 Ste 108,
Oviedo, FL 32765

8010 Red Bug

5.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8010 Red Bug
Oviedo, FL 32765

440 W State Road 436

6.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
440 W State Road 436
Altamonte Springs, FL 32714

7457 Aloma Ave Ste 301

6.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7457 Aloma Ave Ste 301
Winter Park, FL 32792

5931 Brick Court Suite 100

6.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5931 Brick Court Suite 100
Winter Park, FL 32792

755 Rinehart Rd Ste 150

6.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
755 Rinehart Rd Ste 150
Lake Mary, FL 32746

7480 Aloma Ave

6.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7480 Aloma Ave
Winter Park, FL 32792

5931 Aloma Ave Ste 100

6.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5931 Aloma Ave Ste 100
Winter Park, FL 32792

3099 Aloma Ave

6.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3099 Aloma Ave
Winter Park, FL 32792

901 N Lake Destiny Rd Ste 400

6.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
901 N Lake Destiny Rd Ste 400
Maitland, FL 32751

1125 Townpark Ave Ste 1011

6.87 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1125 Townpark Ave Ste 1011
Lake Mary, FL 32746

4122 Metric Dr Ste 800

6.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4122 Metric Dr Ste 800
Winter Park, FL 32792

611 N Wymore Rd Ste 220

7.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
611 N Wymore Rd Ste 220
Winter Park, FL 32789

501 N Orlando Ave Ste 227

7.46 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
501 N Orlando Ave Ste 227
Winter Park, FL 32789

1850 Lee Rd Ste 216

7.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1850 Lee Rd Ste 216
Winter Park, FL 32789

1850 Lee Rd Ste 300

7.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1850 Lee Rd Ste 300
Winter Park, FL 32789

2540 Lee Rd

7.83 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2540 Lee Rd
Winter Park, FL 32789

4451 W State Road 46

7.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4451 W State Road 46
Sanford, FL 32771

1511 E State Road 434 Ste 2001

8.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1511 E State Road 434 Ste 2001
Winter Springs, FL 32708

910 Lexington Green Lane

8.04 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM (Lunch: 12:00 PM - 12:30 PM)⚡ Drug Screens: Mon–Fri: 06:30 AM - 02:30 PM
910 Lexington Green Lane
Sanford, FL 32771

3840 E Semoran Blvd Ste 1000

8.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3840 E Semoran Blvd Ste 1000
Apopka, FL 32703

1338 Pleasant Oak Ln

8.74 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1338 Pleasant Oak Ln
Orlando, FL 32804

5021 Eggleston Ave Ste A

8.79 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5021 Eggleston Ave Ste A
Orlando, FL 32804

324 E Par St.., Ste 100,

8.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
324 E Par St.., Ste 100,
Orlando, FL 32804

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 37 DOT drug testing locations to serve you in and around Winter Springs, FL.

In Winter Springs, FL, our DOT facilities cater to a wide array of services such as DOT Drug and Alcohol Testing, DOT Physicals, and more, all essential for businesses and individuals under 49 CFR Part 40, including FMCSA, FTA, FAA, USCG, FRA, and PHMSA regulations. Our testing centers are conveniently located near you, with same-day services often available.

For DOT-related needs, simply choose your required service or test and find a nearby center. Whether it's for you or others, our intuitive and seamless process ensures you can easily schedule DOT drug testing locally.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Winter Springs, Florida DOT Services

DOT Drug Testing & Services Available in Winter Springs, FL:

Accredited Drug Testing in Winter Springs, FL, offers comprehensive DOT services suitable for various needs, ensuring that businesses and individuals adhere to federal safety requirements efficiently and effectively.

DOT Services in Winter Springs, FL

DOT regulations mandate stringent drug and alcohol screenings to uphold safety in workplaces, especially in sensitive sectors. In Winter Springs, Florida, we help businesses align with federal standards to protect employees and passengers.

Companies operating in Winter Springs, FL, must adhere to DOT regulations which aid in creating a safe work environment. Employee testing for alcohol and drugs is pivotal to meet compliance and safety obligations.

The stringent screening processes mandated by DOT ensure that workers are fit for their jobs, reducing risks and fostering secure operations. Winter Springs, FL, is served well by providers ensuring these standards are met.

Protecting the workforce in Winter Springs, Florida, through DOT compliance involves not just testing but education about regulations. This helps in reducing workplace incidents and ensuring public safety.

FMCSA Information

FMCSA rules in Winter Springs, FL, are critical in maintaining road safety. Accredited Drug Testing ensures that all drivers comply with mandatory drug and alcohol testing.

Compliance with FMCSA drug testing requirements is essential to operate heavy vehicles safely in Winter Springs, Florida.

USCG Information

USCG's stringent drug and alcohol testing rules ensure maritime safety. Accredited Drug Testing in Winter Springs, FL, provides crucial services to meet these requirements, ensuring compliance.

Marine operations in Winter Springs, Florida thrive on adhering to USCG testing standards, safeguarding against potential maritime incidents.

FAA Information

FAA regulations require strict drug and alcohol testing to ensure aviation safety in Winter Springs, Florida. Our testing services support airlines and aircraft operators in complying with these standards.

Adhering to FAA guidelines ensures both crew and passenger safety, making consistent testing services in Winter Springs, FL critical for compliance.

FRA Information

The FRA rules for drug and alcohol testing contribute significantly to railroad safety. In Winter Springs, Florida, our services support compliance with these essential regulations.

Achieving safe rail operations in Winter Springs, FL involves strict adherence to FRA testing guidelines, ensuring workers are fit for duty.

FTA Information

FTA regulations require comprehensive testing services to secure public transit safety. In Winter Springs, Florida, Accredited Drug Testing is pivotal in delivering these services, ensuring compliance.

Public transportation entities in Winter Springs, FL rely on adhering to FTA guidelines to maintain passenger and worker safety.

PHMSA Information

PHMSA drug and alcohol testing rules in Winter Springs, FL, are designed to ensure the security of hazardous material transportation. Compliance is crucial for safety.

In Winter Springs, Florida, Accredited Drug Testing provides essential services to meet PHMSA's stringent screening processes.

Scheduling in Winter Springs, FL

To arrange any DOT drug or alcohol assessment in Winter Springs, FL, call our local office at (800) 221-4291. Alternatively, use our online platform to select your test and fill out the registration section before heading to the testing location.

Use the provided ZIP code to identify the nearest testing site. You'll receive a pass with our location details via email. Walk-ins are welcome but complete the registration and payment online beforehand.

Our facilities hold SAMHSA certification, and results are reviewed by our Medical Review Officers. This rigorous protocol ensures reliability and accuracy across our testing services.

In addition to Winter Springs, FL, Accredited Drug Testing operates in numerous surrounding communities, expanding our reach throughout the metropolitan area.

Wherever you are—on vacation or traveling—we ensure your testing needs are met with our nationwide centers, available at your convenience.

Florida Dept. of Transportation Updates

DUI Statistics in Winter Springs, FL

Winter Springs, Florida is one of the many cities in the Sunshine State where driving under the influence constitutes a significant concern. The Florida Department of Transportation (FDOT) actively works with local law enforcement in Winter Springs, as well as in the larger Seminole County, to address the issue of DUI offenses. Efforts include breathalyzer checkpoints and community awareness campaigns aimed at reducing the rate of incidents. The FDOT reports that DUI incidents contribute to a substantial percentage of traffic-related fatalities and injuries throughout the state, including in Winter Springs. Ongoing education initiatives are crucial in mitigating these occurrences and preserving public safety on Florida's roads.

Drug-Involved Accidents in Winter Springs, FL

In Winter Springs, FL, drug-involved traffic accidents are a recognized problem that the Department of Transportation Florida takes seriously. The FDOT collaborates with local agencies to collect and analyze data on incidents where drugs other than alcohol were a contributing factor. Within Seminole County, which encompasses Winter Springs, law enforcement and public health initiatives aim to track and reduce these occurrences. Educational programs focus on the dangers of driving under the influence of drugs, including prescription medications and illicit substances. The goal is to increase community awareness and encourage safer driving behaviors across Florida, ultimately reducing accident rates.

Marijuana-Related Accidents in Winter Springs, FL

The rise in marijuana-related accidents in Winter Springs, FL, aligns with trends seen throughout Florida. As medical marijuana becomes more accessible and debates around recreational use continue, the Florida Department of Transportation places emphasis on monitoring the impact of cannabis on driving. In Seminole County, officials collaborate with the FDOT to track accidents where marijuana is a factor. Public service campaigns target Winter Springs residents to raise awareness of the impaired driving risks associated with marijuana use. By prioritizing education and implementing targeted safety checks, the FDOT aims to minimize marijuana-related road incidents throughout Florida.

Winter Springs Drug Testing

Quickly find trusted local drug testing centers in Winter Springs, FL — fast, convenient, and reliable every time!

Winter Springs DOT Drug Testing

Quickly find a local DOT drug testing center in Winter Springs, FL — fast, reliable, convenient nationwide service!

Winter Springs DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Winter Springs, FL.

Winter Springs Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Winter Springs, FL.v

Winter Springs Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Winter Springs, FL locations—results you can trust, every time!

Winter Springs Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Winter Springs, FL.

Winter Springs Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Winter Springs, FL.

Winter Springs 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Winter Springs, FL employers and individuals nationwide.

Winter Springs 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Winter Springs, FL workplace and personal testing needs.

Winter Springs On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Winter Springs, FL workplace compliant.

Winter Springs DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Winter Springs, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291