DOT Drug Testing - Ames, IA

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 31 testing centers in the Ames area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

31 DOT Drug-Testing Centers in Ames

Minutes Away, Test Today

1215 Duff Ave

0.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1215 Duff Ave
Ames, IA 50010

1111 Duff Ave

0.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1111 Duff Ave
Ames, IA 50010

1015 Duff Ave

0.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1015 Duff Ave
Ames, IA 50010

809 Wheeler St Ste 104

1.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
809 Wheeler St Ste 104
Ames, IA 50010

207 Stanton Ave

1.74 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
207 Stanton Ave
Ames, IA 50014

630 6th St

8.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
630 6th St
Nevada, IA 50201

640 S 19th St

9.74 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
640 S 19th St
Nevada, IA 50201

1115 S Marshall St

13.27 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
1115 S Marshall St
Boone, IA 50036

1015 Union St

13.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1015 Union St
Boone, IA 50036

1824 Sw White Birch Cir

23.06 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
1824 Sw White Birch Cir
Ankeny, IA 50023

1810 Sw White Birch Circle Ste 111

23.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1810 Sw White Birch Circle Ste 111
Ankeny, IA 50023

5731 Greendale Rd Ste 100

26.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5731 Greendale Rd Ste 100
Johnston, IA 50131

5911 Meredith Dr Ste D

27.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5911 Meredith Dr Ste D
Des Moines, IA 50322

1650 E Madison Ave

27.77 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1650 E Madison Ave
Des Moines, IA 50313

3770 8th St Sw

27.80 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
3770 8th St Sw
Altoona, IA 50009

610 10th St

28.09 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
610 10th St
Perry, IA 50220

2340 Euclid Ave

28.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2340 Euclid Ave
Des Moines, IA 50310

11144 Aurora Ave

28.52 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
11144 Aurora Ave
Urbandale, IA 50322

11319 Aurora Ave,

28.53 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
11319 Aurora Ave,
Urbandale, IA 50322

11208 Aurora Ave Bldg 12

28.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11208 Aurora Ave Bldg 12
Urbandale, IA 50322

2100 Dixon St Ste E

29.17 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
2100 Dixon St Ste E
Des Moines, IA 50316

1301 Pennsylvania Ave Ste 416

29.90 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1301 Pennsylvania Ave Ste 416
Des Moines, IA 50316

507 14th St

29.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
507 14th St
Dallas Center, IA 50063

2417 University Ave

30.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2417 University Ave
Des Moines, IA 50311

2350 Hospital Dr

30.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2350 Hospital Dr
Webster City, IA 50595

1200 Pleasant St

30.74 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1200 Pleasant St
Des Moines, IA 50309

2700 Westown Pkwy Ste 270

30.94 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2700 Westown Pkwy Ste 270
West Des Moines, IA 50266

800 Ohio St

31.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
800 Ohio St
Webster City, IA 50595

1233 8th St

31.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1233 8th St
West Des Moines, IA 50265

914 Willson Ave

31.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
914 Willson Ave
Webster City, IA 50595

12871 University Ave Ste 130

31.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12871 University Ave Ste 130
Clive, IA 50325

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 31 DOT drug testing locations to serve you in and around Ames, IA.

Located in Ames, IA, our DOT Drug Testing facilities ensure compliance with 49 CFR Part 40, offering tests and services like DOT Physicals, Random pool, and Consortium services covering FMCSA, FAA, and others. We have convenient and swift testing centers, many providing same-day availability.

Choose your required DOT or drug/alcohol tests and a location nearby. Testing is accessible for individuals, employees, or others, made easier with our streamlined process.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Ames, Iowa DOT Services

DOT Drug Testing & Services Available in Ames, IA:

Accredited Drug Testing provides comprehensive DOT services in Ames, IA, including all necessary drug and alcohol testing to ensure compliance and safety for your organization and its employees.

DOT Services in Ames, IA

DOT regulations ensure that employers in Ames, Iowa conduct regular drug and alcohol screenings to promote a safer workplace. Compliance with these guidelines helps maintain overall safety and efficiency.

Drug and alcohol screenings must be carried out in accordance with DOT regulations, ensuring safety for all employees, particularly those in safety-sensitive positions within Ames, Iowa.

manual-labor or safety-critical roles, employees must submit to periodic testing as instructed by DOT mandates in Ames, Iowa, ensuring workplace integrity and personnel safety.

Employers in Ames, Iowa must keep consistent records of all testing procedures under DOT guidelines, helping to verify compliance and promoting transparency and safety.

FMCSA Information

In Ames, Iowa, the FMCSA imposes strict drug and alcohol testing rules for drivers, ensuring safer roads and work environments. Compliance with these regulations is mandatory.

Employers in Ames, IA, must adhere to FMCSA testing regulations, conducting pre-employment, random, and post-accident testing to ensure safety and compliance.

USCG Information

USCG emphasizes alcohol and drug testing for maritime workers in Ames, IA to ensure vessel safety and environmental protection.

Mariners in Ames, Iowa engage in USCG-compliant testing to uphold safety regulations and prevent alcohol and drug-related incidents.

FAA Information

FAA regulations necessitate drug and alcohol testing for aviation employees in Ames, IA to maintain safety and operational integrity across airports and air carriers.

Pilots and aviation crew in Ames, Iowa comply with FAA testing regulations, involving random and periodic checks to ensure strict adherence to safety standards.

FRA Information

FRA emphasizes the importance of drug and alcohol compliance for railway employees in Ames, IA, mandating testing to maintain rail safety and service reliability.

Employees under FRA jurisdiction in Ames, Iowa, undergo mandatory testing to align with established drug and alcohol regulations for safe railway operations.

FTA Information

FTA rules require strict drug and alcohol testing for transit employees in Ames, Iowa, ensuring the safety and reliability of public transportation services.

In Ames, IA, transit staff are subject to rigorous testing as per FTA guidelines to promote commuter safety and operational excellence.

PHMSA Information

PHMSA regulations in Ames, IA focus on maintaining safety by implementing rigorous drug and alcohol testing policies for pipeline operators and other related sectors.

In Ames, Iowa, PHMSA mandates random drug testing to ensure that operators handling hazardous materials remain alert, sober, and compliant.

Scheduling in Ames, IA

For DOT drug or alcohol tests in Ames, IA, schedule through our local department at (800) 221-4291 or our online registration system. Ensure you register before visiting the testing center.

Enter your zip code to find the nearest testing center in Ames, Iowa. A donor pass with details will be emailed to you. Typically, appointments are not required, but registration and payment must be completed beforehand.

Our labs, accredited by SAMHSA, provide test results verified by licensed MROs.

Beyond Ames, IA, Accredited Drug Testing has centers throughout the metro and nearby areas.

Whether in town or away, Accredited Drug Testing offers nationwide testing centers available at your need: anywhere, anytime!

Iowa Dept. of Transportation Updates

DUI Statistics in Ames, IA

Driving Under the Influence (DUI) remains a significant concern for the Department of Transportation (DOT) in both Ames, IA and the broader Story County region. According to Iowa state data, alcohol-related incidents are a major contributor to vehicular accidents, often exacerbated by factors like age, driving habits, and time of day. In Ames, IA, local law enforcement and DOT initiatives focus heavily on reducing DUI occurrences through increased patrols and public awareness campaigns. Iowa State University, located in Ames, plays a pivotal role in supporting these efforts by educating students on safe driving practices. Collaborative programs emphasize the importance of designated drivers and the availability of alternative transportation options to curb impaired driving. These initiatives are crucial in maintaining public safety and reducing accident rates caused by impaired drivers in Ames, IA.

Drug-Involved Accidents in Ames, IA

Accidents involving drug influence pose a significant challenge for transportation safety in Ames, IA, and throughout Story County. The Iowa Department of Transportation (DOT) recognizes that drug-related crashes often result in severe outcomes, putting lives at risk. In Ames, comprehensive studies have shown that various controlled substances can impair drivers' abilities to operate vehicles safely. Collaborative efforts with Ames law enforcement aim to crack down on drug-impaired driving through checkpoints and specialized training for officers to recognize signs of drug use. Moreover, state and local authorities work together to raise public awareness of the risks associated with drug-impaired driving. These joint efforts aim to create a safer transportation environment in Ames, IA, aligning with Iowa's broader goals to reduce drug-influenced road incidents.

Marijuana-Related Accidents in Ames, IA

The legalization and increased societal acceptance of marijuana have led to growing concerns about its impact on road safety in Ames, IA, and Story County. The Iowa Department of Transportation (DOT) has been actively monitoring and addressing the challenges posed by marijuana-influenced driving. Data from Ames illustrates a nuanced relationship between marijuana legalization and traffic incidents, prompting local initiatives to better understand and mitigate its effects. Partnerships with local government and health organizations in Ames aim to educate the public about the dangers of driving under the influence of marijuana. Detection and deterrence, such as roadside testing and awareness campaigns, play a crucial role in Ames, IA's strategy to reduce marijuana-related accidents. These efforts are essential to ensuring road safety and align with Iowa's broader commitment to reducing substance-influenced accidents.

Ames Drug Testing

Quickly find trusted local drug testing centers in Ames, IA — fast, convenient, and reliable every time!

Ames DOT Drug Testing

Quickly find a local DOT drug testing center in Ames, IA — fast, reliable, convenient nationwide service!

Ames DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Ames, IA.

Ames Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Ames, IA.v

Ames Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Ames, IA locations—results you can trust, every time!

Ames Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Ames, IA.

Ames Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Ames, IA.

Ames 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Ames, IA employers and individuals nationwide.

Ames 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Ames, IA workplace and personal testing needs.

Ames On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Ames, IA workplace compliant.

Ames DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Ames, IA drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

Super Helpful

Detricia was super helpful in making sure that the drug test is sent my worker for was the right one I needed for my company to be compliant with my customers drug test requirements.

Kevin Howell - 12/19/2024

Very professional and very quick

Very professional and very quick service to schedule appointments! I am pleased with the services and would definitely recommend using them for any drug testing needs!

Carrie Feerick - 4/19/2025

Quick, knowledgeable and courteous

Quick, knowledgeable and courteous Janelle worked diligently to support me. I am recommending their services to anyone looking.

Greensboro Joseph - 11/19/2024


(800) 221-4291