DOT Drug Testing - Middlesboro, KY

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 35 testing centers in the Middlesboro area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

35 DOT Drug-Testing Centers in Middlesboro

Minutes Away, Test Today

2317 Cumberland Ave

0.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2317 Cumberland Ave
Middlesboro, KY 40965

123 N 19th St

0.26 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM (Lunch: 12:00 PM - 12:30 PM)⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 01:30 PM - 03:00 PM
123 N 19th St
Middlesboro, KY 40965

Us Hwy 58

3.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Us Hwy 58
Ewing, VA 24248

170 Beech St Ste 1

3.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
170 Beech St Ste 1
Harrogate, TN 37752

169 Westmoreland St

3.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
169 Westmoreland St
Harrogate, TN 37752

121 W Virginia Ave

10.65 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
121 W Virginia Ave
Pineville, KY 40977

309 N Broad St

13.16 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-11:00 AM & 1:00 PM-3:00 PM✓ Walk-in with Electronic Donor Pass
309 N Broad St
New Tazewell, TN 37825

1850 Old Knoxville Rd

13.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1850 Old Knoxville Rd
Tazewell, TN 37879

1601 Tazewell Rd

13.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1601 Tazewell Rd
Tazewell, TN 37879

80 Hospital Dr

18.88 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
80 Hospital Dr
Barbourville, KY 40906

980 Hwy 3449

20.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
980 Hwy 3449
Coldiron, KY 40819

120 Professional Ln Ste 101

22.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
120 Professional Ln Ste 101
Harlan, KY 40831

589 W Highway 92

26.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
589 W Highway 92
Williamsburg, KY 40769

998 S Hwy 25 W

26.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
998 S Hwy 25 W
Williamsburg, KY 40769

919 E Central Ave Ste 101

26.77 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM (Lunch: 12:30 PM - 01:30 PM)⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:30 AM, 01:30 PM - 03:30 PM
919 E Central Ave Ste 101
La Follette, TN 37766

923 E Central Ave

26.77 mi
🕑 Facility: Mon–Fri: 08:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 12:00 PM, 02:00 PM - 04:00 PM
923 E Central Ave
La Follette, TN 37766

905 E Central Ave

26.77 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 03:00 PM
905 E Central Ave
La Follette, TN 37766

123 E Central St

27.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
123 E Central St
Harlan, KY 40831

1519 Main St

28.34 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM✓ Walk-in with Electronic Donor Pass
1519 Main St
Sneedville, TN 37869

2702 Jacksboro Pike

29.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2702 Jacksboro Pike
Jacksboro, TN 37757

2503 Jacksboro Pike

29.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2503 Jacksboro Pike
Jacksboro, TN 37757

2305 Jacksboro Pike

30.13 mi
🕑 Facility: Mon–Fri: 08:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 11:30 AM, 01:30 PM - 04:30 PM
2305 Jacksboro Pike
La Follette, TN 37766

1321 Cumberland Falls Highway,

30.97 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
1321 Cumberland Falls Highway,
Corbin, KY 40701

1 Trillium Way

31.07 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
1 Trillium Way
Corbin, KY 40701

121 Bishop St

31.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
121 Bishop St
Corbin, KY 40701

3170 Appalachian Hwy Ste 5

32.47 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3170 Appalachian Hwy Ste 5
Jacksboro, TN 37757

60 S Stewart Rd

33.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
60 S Stewart Rd
Corbin, KY 40701

South Hwy 25

33.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
South Hwy 25
Lily, KY 40740

502 W 7th North St Lower Level

33.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
502 W 7th North St Lower Level
Morristown, TN 37814

101 Chad St

34.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
101 Chad St
Evarts, KY 40828

2412 W Andrew Johnson Hwy

35.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2412 W Andrew Johnson Hwy
Morristown, TN 37814

850 W 3rd North St Ste A

35.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
850 W 3rd North St Ste A
Morristown, TN 37814

1171 Highway 11 E Ste 101

35.82 mi
🕑 Facility: Mon–Fri: 07:30 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 04:00 PM
1171 Highway 11 E Ste 101
Talbott, TN 37877

95 Bryan Blvd Ste 201

35.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
95 Bryan Blvd Ste 201
Corbin, KY 40701

1633 W Morris Blvd Ste A

35.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1633 W Morris Blvd Ste A
Morristown, TN 37813

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 35 DOT drug testing locations to serve you in and around Middlesboro, KY.

Our DOT drug and alcohol testing centers in Middlesboro, KY, and nearby areas offer a comprehensive range of services, including DOT Physicals, Random Pool enrollment, and reports for various DOT modes such as FMCSA, FTA, and others compliant with 49 CFR Part 40. Conveniently situated testing facilities ensure quick access and, typically, same-day service.

Choose the desired DOT service or test, and select a location close to you. Our efficient process allows seamless scheduling of DOT drug testing in Middlesboro, facilitating easy access for individuals or employees.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Middlesboro, Kentucky DOT Services

DOT Drug Testing & Services Available in Middlesboro, KY:

Accredited Drug Testing offers dependable DOT services in Middlesboro, KY, ensuring compliance with federal regulations through a range of testing options, all designed to maintain workplace safety and integrity.

DOT Services in Middlesboro, KY

Ensuring compliance with DOT regulations, Accredited Drug Testing provides reliable drug and alcohol screening to promote workplace safety. In Middlesboro, KY, adherence to these regulations is paramount for operational success.

DOT mandates ensure that employees in safety-sensitive positions undergo regular testing to maintain a drug-free workplace. Middlesboro, Kentucky businesses must comply with these standards to protect their workforce.

Employers in Middlesboro, KY can rely on Accredited Drug Testing to execute DOT screenings that conform to federal and state laws, preserving both safety and compliance.

By adhering to DOT guidelines, companies in Middlesboro, Kentucky can foster an environment prioritizing health and safety, thereby enhancing overall workplace integrity and productivity.

FMCSA Information

In Middlesboro, KY, FMCSA rules require mandatory drug and alcohol testing for commercial drivers to ensure safety on public roads.

Accredited Drug Testing assists Middlesboro, Kentucky companies in adhering to FMCSA guidelines for a safe driving environment.

USCG Information

The USCG's protocols in Middlesboro, KY involve regular testing to ensure maritime safety and operational trustworthiness.

Complying with USCG rules, workers in Middlesboro, Kentucky ensure vigilance and safety in marine operations.

FAA Information

The FAA mandates in Middlesboro, KY require aviation personnel to submit to regular testing, ensuring airport safety.

Employees in Middlesboro, Kentucky's aviation sector are held to stringent FAA standards to maintain operational security.

FRA Information

The FRA enforces strict testing for railway workers in Middlesboro, KY to ensure compliance and safe train operations.

In Middlesboro, Kentucky, compliance with FRA drug testing regulations is essential for maintaining safe rail services.

FTA Information

FTA mandates in Middlesboro, KY focus on testing transit employees to protect public safety effectively.

Public transportation workers in Middlesboro, Kentucky support FTA's ethical standards through regular testing.

PHMSA Information

PHMSA regulations in Middlesboro, KY, ensure pipeline operators undergo rigorous drug and alcohol testing to maintain safety.

Middlesboro, Kentucky personnel adhering to PHMSA rules help prevent incidents through stringent substance abuse reduction practices.

Scheduling in Middlesboro, KY

To schedule any DOT drug or alcohol test in Middlesboro, KY, contact us at (800) 221-4291 or use our online express registration by selecting your test and completing the Donor Information section. Registration is required before visiting the center.

Upon entering your zip code, the nearest center offering your selected test in Middlesboro, KY, will be identified. You'll receive a registration form via email including center details. Bring it with you—appointments are usually unnecessary, but registration payment is due at that time.

Our labs meet SAMHSA certification standards, and results are verified by our licensed Medical Review Officers (MRO) to ensure accuracy.

Accredited Drug Testing provides DOT drug and alcohol testing in Middlesboro, KY, as well as in various other locations across the metropolitan area.

With numerous testing centers nationwide, Accredited Drug Testing guarantees accessibility for your test needs, no matter where you are.

Kentucky Dept. of Transportation Updates

DUI Statistics in Middlesboro, KY

The city of Middlesboro, located in Bell County, Kentucky, has been closely monitoring DUI (Driving Under the Influence) incidents as they remain a significant safety concern. Kentucky law strictly prohibits driving with a blood alcohol concentration (BAC) of 0.08% or higher, and Middlesboro, KY, enforces this regulation rigorously. Recently, efforts by law enforcement and community programs have been directed at reducing these offenses through increased patrols, awareness campaigns, and collaboration with local organizations. Although Middlesboro represents just a small fraction of Kentucky's overall DUI statistics, its efforts contribute to a broader state initiative to enhance road safety and reduce impaired driving incidents across KY, impacting not only Middlesboro but also surrounding communities.

Drug Involved Accidents in Middlesboro, KY

Middlesboro, Kentucky, has observed a concerning trend in drug-involved accidents, reflecting a larger issue across Bell County and the state. Kentucky officials, including those in Middlesboro, KY, are working to identify and mitigate the risks posed by drivers impaired by prescription medications, illegal drugs, and other substances. Local authorities have increased educational efforts to inform citizens of the dangers of drug-impaired driving and have implemented stricter law enforcement protocols to address these incidents. This proactive stance in Middlesboro, KY, aligns with statewide initiatives to decrease the prevalence of such accidents, aiming to improve public safety and foster a culture of responsibility on Kentucky's roads.

Marijuana Related Accidents in Middlesboro, KY

In Middlesboro, Kentucky, there is an ongoing dialogue about the implications of marijuana legalization and its potential impact on road safety. Although marijuana is not currently legal for recreational use in Kentucky, law enforcement in Middlesboro, KY, remains vigilant about the possible influence of the drug on driving behavior. Studies and reports indicate a gradual increase in marijuana-related accidents across the state, prompting local initiatives to collect data and increase community awareness. Middlesboro is actively participating in statewide discussions to address these concerns, focusing on enhancing drug detection training for officers and promoting educational campaigns that highlight the dangers of driving under the influence of marijuana.

Middlesboro Drug Testing

Quickly find trusted local drug testing centers in Middlesboro, KY — fast, convenient, and reliable every time!

Middlesboro DOT Drug Testing

Quickly find a local DOT drug testing center in Middlesboro, KY — fast, reliable, convenient nationwide service!

Middlesboro DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Middlesboro, KY.

Middlesboro Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Middlesboro, KY.v

Middlesboro Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Middlesboro, KY locations—results you can trust, every time!

Middlesboro Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Middlesboro, KY.

Middlesboro Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Middlesboro, KY.

Middlesboro 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Middlesboro, KY employers and individuals nationwide.

Middlesboro 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Middlesboro, KY workplace and personal testing needs.

Middlesboro On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Middlesboro, KY workplace compliant.

Middlesboro DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Middlesboro, KY drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291