Drug Test Services - Middlesboro, KY

Urine | Hair | DOT | Oral Fluid | Alcohol/ETG | Occupational Health

With 35 testing centers in the Middlesboro area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

35 Drug Test Service Centers in Middlesboro

Minutes Away, Test Today

2317 Cumberland Ave

0.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2317 Cumberland Ave
Middlesboro, KY 40965

123 N 19th St

0.26 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM (Lunch: 12:00 PM - 12:30 PM)⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 01:30 PM - 03:00 PM
123 N 19th St
Middlesboro, KY 40965

Us Hwy 58

3.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Us Hwy 58
Ewing, VA 24248

170 Beech St Ste 1

3.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
170 Beech St Ste 1
Harrogate, TN 37752

169 Westmoreland St

3.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
169 Westmoreland St
Harrogate, TN 37752

121 W Virginia Ave

10.65 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
121 W Virginia Ave
Pineville, KY 40977

309 N Broad St

13.16 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-11:00 AM & 1:00 PM-3:00 PM✓ Walk-in with Electronic Donor Pass
309 N Broad St
New Tazewell, TN 37825

1850 Old Knoxville Rd

13.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1850 Old Knoxville Rd
Tazewell, TN 37879

1601 Tazewell Rd

13.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1601 Tazewell Rd
Tazewell, TN 37879

80 Hospital Dr

18.88 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
80 Hospital Dr
Barbourville, KY 40906

980 Hwy 3449

20.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
980 Hwy 3449
Coldiron, KY 40819

120 Professional Ln Ste 101

22.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
120 Professional Ln Ste 101
Harlan, KY 40831

589 W Highway 92

26.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
589 W Highway 92
Williamsburg, KY 40769

998 S Hwy 25 W

26.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
998 S Hwy 25 W
Williamsburg, KY 40769

919 E Central Ave Ste 101

26.77 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM (Lunch: 12:30 PM - 01:30 PM)⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:30 AM, 01:30 PM - 03:30 PM
919 E Central Ave Ste 101
La Follette, TN 37766

923 E Central Ave

26.77 mi
🕑 Facility: Mon–Fri: 08:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 12:00 PM, 02:00 PM - 04:00 PM
923 E Central Ave
La Follette, TN 37766

905 E Central Ave

26.77 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 03:00 PM
905 E Central Ave
La Follette, TN 37766

123 E Central St

27.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
123 E Central St
Harlan, KY 40831

1519 Main St

28.34 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM✓ Walk-in with Electronic Donor Pass
1519 Main St
Sneedville, TN 37869

2702 Jacksboro Pike

29.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2702 Jacksboro Pike
Jacksboro, TN 37757

2503 Jacksboro Pike

29.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2503 Jacksboro Pike
Jacksboro, TN 37757

2305 Jacksboro Pike

30.13 mi
🕑 Facility: Mon–Fri: 08:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 11:30 AM, 01:30 PM - 04:30 PM
2305 Jacksboro Pike
La Follette, TN 37766

1321 Cumberland Falls Highway,

30.97 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
1321 Cumberland Falls Highway,
Corbin, KY 40701

1 Trillium Way

31.07 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
1 Trillium Way
Corbin, KY 40701

121 Bishop St

31.55 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
121 Bishop St
Corbin, KY 40701

3170 Appalachian Hwy Ste 5

32.47 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3170 Appalachian Hwy Ste 5
Jacksboro, TN 37757

60 S Stewart Rd

33.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
60 S Stewart Rd
Corbin, KY 40701

South Hwy 25

33.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
South Hwy 25
Lily, KY 40740

502 W 7th North St Lower Level

33.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
502 W 7th North St Lower Level
Morristown, TN 37814

101 Chad St

34.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
101 Chad St
Evarts, KY 40828

2412 W Andrew Johnson Hwy

35.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2412 W Andrew Johnson Hwy
Morristown, TN 37814

850 W 3rd North St Ste A

35.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
850 W 3rd North St Ste A
Morristown, TN 37814

1171 Highway 11 E Ste 101

35.82 mi
🕑 Facility: Mon–Fri: 07:30 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 04:00 PM
1171 Highway 11 E Ste 101
Talbott, TN 37877

95 Bryan Blvd Ste 201

35.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
95 Bryan Blvd Ste 201
Corbin, KY 40701

1633 W Morris Blvd Ste A

35.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1633 W Morris Blvd Ste A
Morristown, TN 37813

Employers | Individuals | Families

Accredited Drug Testing provides comprehensive drug and alcohol testing services to promote workplace safety, meet compliance requirements, and support legal or personal needs for testing. Drug Testing helps prevent accidents, ensures a drug-free workplace,and meets Federal mandates such as those outlined by the Department of Transportation (DOT)

Drug Testing Services

Customized Testing Options Available | Discounts available for Bulk Purchases | Onsite Drug Testing Services

Urine Drug Testing Services

Urine drug testing is a common method used to detect the presence of drugs or their metabolites in an individual's system. This non-invasive testing procedure is widely employed in various settings due to its accuracy and reliability.

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Purpose of Urine Drug Testing

  • Pre-employment screening
  • Compliance with legal requirements
  • Monitoring treatment programs
  • Ensuring workplace safety
  • Sports regulations adherence
  • Parental supervision tool

What Drugs Can Be Detected

  • Marijuana
  • Cocaine
  • Opiates
  • Amphetamines
  • Benzodiazepines
  • Phencyclidine (PCP)

In Middlesboro, Kentucky, urine drug testing is essential in maintaining a substance-free environment, providing peace of mind for employers and families alike.

Order Your Test Online

Alcohol Test Services

Alcohol testing in Middlesboro, Kentucky is designed to measure the level of alcohol in a person’s system and determine any impairment. Various industries rely on alcohol testing to ensure safety and compliance with regulations.

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Alcohol Testing is used in many settings

  • Workplace safety
  • Transportation industry
  • Refinery operations
  • Law enforcement
  • Court orders
  • Rehabilitation programs

Common Methods of Alcohol Testing

  • Breathalyzer
  • Blood tests
  • Saliva tests
  • Urine tests
  • Hair tests

Breathalyzer - Commonly used by law enforcement for roadside testing.

Blood tests - Provide accurate BAC levels in legal settings.

Saliva tests - Quick and non-invasive; used in several industries.

Urine tests - Detects alcohol consumption within the past few days.

Hair tests - Used for long-term detection of alcohol consumption.

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Hair Follicle Drug Testing Services

Hair follicle drug testing in Middlesboro, Kentucky offers a nuanced approach for detecting illicit substances by analyzing hair samples, providing an extended detection window.

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Why Use Hair Drug Testing?

  • Extended detection period
  • Non-invasive collection
  • Difficult to adulterate samples
  • Reflects chronic drug use
  • Suitable for pre-employment checks
  • Reliable legal evidence

What Drugs Can Be Detected

  • Cocaine
  • Marijuana
  • Opiates
  • Methamphetamines
  • Ecstasy
  • Phencyclidine (PCP)

In Middlesboro, Kentucky, hair follicle testing is a preferred method for its accuracy, providing a comprehensive overview of an individual's substance use history.

Order Your Test Online

DOT Drug Testing Services

DOT drug testing in Middlesboro, Kentucky is a federally mandated program that ensures public safety by testing transportation employees for drug use. The process involves random screenings, pre-employment tests, and other evaluations to maintain compliance with safety regulations. DOT testing aims to prevent substance-related incidents on public transport.

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Who is Required to Take DOT Drug Tests?

  • Commercial drivers
  • Aviation personnel
  • Pipeline workers
  • Railroad employees
  • Transit operators
  • Maritime crew members

What Drugs Are Tested For

  • Marijuana
  • Cocaine
  • Amphetamines
  • Opiates
  • PCP

Conducting DOT drug tests in Middlesboro, Kentucky plays a crucial role in safeguarding our transit systems by ensuring employees are sober and qualified, significantly reducing the risk of accidents and enhancing overall safety.

Order Your Test Online

Probation Drug Testing Services

Probation drug testing in , CO involves assessing probationers for illegal substance use as part of compliance monitoring. This testing is typically mandated by the court and involves various biological samples to ensure individuals remain substance-free throughout their probation.

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Why Are Probation Drug Test Services Available?

  • Ensure compliance: Verifies adherence to probation terms.
  • Rehabilitation support: Encourages sobriety.
  • Public safety: Reduces substance abuse consequences.
  • Prevent recidivism: Discourages repeat offenses.
  • Accountability: Holds individuals responsible.
  • Monitoring progression: Tracks rehabilitative progress.

Testing Methods

  • Urine tests: Most common for general screening.
  • Hair follicle tests: Extended detection window.
  • Saliva tests: Immediate detection for recent use.
  • Blood tests: Highly accurate for specific scenarios.

Probation drug testing in Middlesboro, Kentucky ensures that individuals adhere to legal requirements and contribute positively to their communities.

Order Your Test Online

Court Ordered Drug Testing Services

Court-ordered drug testing in , CT is mandated by judges to ensure individuals maintain a drug-free lifestyle. The process involves testing an individual’s biological samples in laboratories to detect illegal substance use.

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Why are Court Ordered Drug Test Services Available?

  • Ensuring compliance with legal rulings
  • Monitoring parolees
  • Supporting rehabilitation programs
  • Protecting public safety
  • Providing evidence in legal proceedings

Court Ordered Test Methods

  • Urine tests: Standard for immediate detection.
  • Hair follicle tests: Detects long-term drug use.
  • Saliva tests: For recent drug intake analysis.
  • Blood tests: Provides detailed substance information.
  • Nail tests: Captures extended usage history.

Court ordered drug testing in Middlesboro, Kentucky assists the judiciary in managing drug-related cases effectively, supporting both compliance and rehabilitation goals while maintaining public safety.

Order Your Test Online

Fingernail Drug Testing Services

Fingernail drug testing provides a forensic measure of substance use by analyzing samples from both fingernails and toenails. This non-invasive test offers insight into long-term drug exposure, as nails capture metabolites over a significant time.

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What is the Detection Window for Fingernail Drug Tests

  • Fingernails: Can reveal drug exposure for up to 6 months
  • Toenails: Often used for a longer detection period of up to 12 months

What Drugs Can Be Detected

  • Marijuana
  • Cocaine
  • Opiates
  • Methamphetamine
  • Benzodiazepines

Advantages over other methods

  • Long detection period
  • Non-invasive and easy to collect
  • Difficult to tamper with
  • Applicable for various substances
  • More cost-effective over the long term

In Middlesboro, Kentucky, fingernail testing offers a valuable option for those needing comprehensive drug testing results. Its reliability and extensive detection window make it a preferred choice for organizations and individuals looking for accurate substance use histories.

Order Your Test Online

Occupational Health Test Services

Occupational health testing in Middlesboro, Kentucky supports workplaces by ensuring that employees are fit for duty and healthy enough to perform job functions safely. It involves a series of evaluations that protect workers from health risks and ensure compliance with industry regulations, enhancing overall workplace safety.

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Purpose of Occupational Health Testing

  • Ensures employee fitness
  • Reduces workplace accidents
  • Identifies health impairments
  • Supports legal compliance
  • Increases productivity

Common Types of Occupational Health Tests

  • Fitness for duty exams: Evaluates physical capability.
  • Respirator Fit Testing: Ensures proper respirator use.
  • Pulmonary Function Tests (PFT's): Assesses lung function.
  • Tuberculosis (TB): Screens for TB infection.
  • Vision: Checks vision health.
  • Audiometric: Monitors hearing capability.

Occupational health testing plays a vital role in ensuring safe, efficient, and compliant operations within Middlesboro, Kentucky workplaces, fostering a productive environment for both employees and employers.

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Drug Statistics for Middlesboro, Kentucky

  • Substance Abuse Rate: The prevalence of substance abuse in Middlesboro aligns with broader state trends in Kentucky, where opioid misuse remains a concern.
  • Overdose Incidents: Similar to national patterns, Middlesboro has seen fluctuations in overdose incidents, reflective of ongoing efforts to combat the opioid crisis.
  • Treatment Access: Middlesboro offers several drug treatment centers and recovery programs aimed at reducing addiction rates and supporting community health.

Drug Testing Regulations in Kentucky

Employer drug testing in Middlesboro must comply with state guidelines under KRS § 336.010 et seq.. Kentucky law permits employers to maintain zero-tolerance drug policies, offering voluntary drug-free workplace program certification and worker compensation safety benefits.

Medical Marijuana Status: Legal (Medical)  |  Recreational Marijuana Status: Prohibited

Surrounding Areas Served Near Middlesboro

Middlesborough • Meldrum • Colmar • Fonde • Pruden-fonde • Pineville • Wheeler • Pearl

Drug & Alcohol Test Selection & Panel Comparison

Test Type / Panel Detection Window Common Applications Turnaround Time
5-Panel Urine Screen 1 to 5 Days Standard Pre-Employment, Random & Workplace Testing 24 to 48 Hours
10-Panel Urine Screen 1 to 7 Days Expanded Employment, Healthcare & Probation 24 to 48 Hours
Hair Follicle Drug Test Up to 90 Days Long-Term Monitoring, Court-Ordered & Custody 3 to 5 Business Days
Oral Fluid / Saliva Screen 1 to 48 Hours Immediate Recent-Use Screening & On-Site Testing 24 to 48 Hours
ETG Alcohol Testing Up to 80 Hours Court-Ordered Alcohol Abstinence & Probation Monitoring 24 to 48 Hours

Middlesboro Drug Testing

Quickly find trusted local drug testing centers in Middlesboro, KY — fast, convenient, and reliable every time!

Middlesboro DOT Drug Testing

Quickly find a local DOT drug testing center in Middlesboro, KY — fast, reliable, convenient nationwide service!

Middlesboro DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Middlesboro, KY.

Middlesboro Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Middlesboro, KY.v

Middlesboro Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Middlesboro, KY locations—results you can trust, every time!

Middlesboro Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Middlesboro, KY.

Middlesboro Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Middlesboro, KY.

Middlesboro 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Middlesboro, KY employers and individuals nationwide.

Middlesboro 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Middlesboro, KY workplace and personal testing needs.

Middlesboro On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Middlesboro, KY workplace compliant.

Middlesboro DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Middlesboro, KY drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Kentucky, workplace drug testing is governed by statutory guidelines under KRS § 336.010 et seq.. Private employers generally operate under a Kentucky voluntary drug-free workplace standards, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Kentucky who implement a certified Drug-Free Workplace Program in compliance with KRS § 336.010 et seq. can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Kentucky state law compliance standards, state statutes allows employers to enforce strict zero-tolerance policies. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 0 certified collection sites in the Middlesboro area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Middlesboro, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

Very Fun and Easy

Trish last week and Tatiana this week, very fun and easy folks to deal with. Well be using them more and more in the future.

Tom O - 12/19/2024

Everything was great

Everything was great, the staff was very polite. Thank you.

Olga Petrova - 9/19/2024

Extremely polite

They were very fast extremely polite walked you through every detail of the process if you had any questions they had no problems answering

Billy Williams - 2/19/2025


(800) 221-4291