DOT Drug Testing - Buffalo, NY

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 39 testing centers in the Buffalo area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

39 DOT Drug-Testing Centers in Buffalo

Minutes Away, Test Today

700 Michigan Ave

0.72 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
700 Michigan Ave
Buffalo, NY 14203

455 Delaware Ave,

0.72 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-1:30 PM • Sat: 6:30 AM-10:00 AM✓ Walk-in with Electronic Donor Pass
455 Delaware Ave,
Buffalo, NY 14202

85 High St

1.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
85 High St
Buffalo, NY 14203

1317 Jefferson Ave,

2.10 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
1317 Jefferson Ave,
Buffalo, NY 14208

699 Hertel Ave Ste 355

4.24 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
699 Hertel Ave Ste 355
Buffalo, NY 14207

155 Lawn Ave,

4.44 mi
🕑 Testing Hours: Mon–Fri: 9:00 AM-2:30 PM✓ Walk-in with Electronic Donor Pass
155 Lawn Ave,
Buffalo, NY 14207

2497 Delaware Ave Inside The Wny Immed. Care

4.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2497 Delaware Ave Inside The Wny Immed. Care
Buffalo, NY 14216

1779 Walden Avenue, Suite 300

5.64 mi
🕑 Testing Hours: Mon–Fri: 5:30 AM-12:30 PM✓ Walk-in with Electronic Donor Pass
1779 Walden Avenue, Suite 300
Cheektowaga, NY 14225

2871 Genesee St

5.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2871 Genesee St
Cheektowaga, NY 14225

1660 Kensington Ave

5.85 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
1660 Kensington Ave
Cheektowaga, NY 14215

3121 Clinton St Ste 6

6.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3121 Clinton St Ste 6
West Seneca, NY 14224

264 Center Road,

6.24 mi
🕑 Testing Hours: Mon–Fri: 5:30 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
264 Center Road,
West Seneca, NY 14224

1900 Ridge Rd

6.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1900 Ridge Rd
West Seneca, NY 14224

3842 Harlem Road,

6.38 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
3842 Harlem Road,
Cheektowaga, NY 14215

3500 Main Street, University Plaza

6.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3500 Main Street, University Plaza
Amherst, NY 14226

2075 Sheridan Dr

6.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2075 Sheridan Dr
Kenmore, NY 14223

1173 Sheridan Dr

6.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1173 Sheridan Dr
Tonawanda, NY 14150

2173 Sheridan Drive,

6.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2173 Sheridan Drive,
Tonawanda, NY 14223

3980 Sheridan Drive - Suite 3, Dent Tower

6.90 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3980 Sheridan Drive - Suite 3, Dent Tower
Amherst, NY 14226

4500 Main St

7.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4500 Main St
Amherst, NY 14226

1106 Union Road, Southgate Plaza

7.23 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM • Sat: 6:00 AM-10:00 AM✓ Walk-in with Electronic Donor Pass
1106 Union Road, Southgate Plaza
West Seneca, NY 14224

3050 Orchard Park Rd Bldg B Located Inside Immediate Care

7.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3050 Orchard Park Rd Bldg B Located Inside Immediate Care
Buffalo, NY 14224

4041 Delaware Avenue, Suite 110

7.46 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-3:00 PM • Sat: 6:00 AM-10:00 AM✓ Walk-in with Electronic Donor Pass
4041 Delaware Avenue, Suite 110
Tonawanda, NY 14150

3620 Sheridan Dr - Suite 100,

7.58 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3620 Sheridan Dr - Suite 100,
Amherst, NY 14226

3050 Orchard Park Road,

8.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3050 Orchard Park Road,
West Seneca, NY 14224

4154 Mckinley Pkwy,

8.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4154 Mckinley Pkwy,
Blasdell, NY 14219

4700 Genesee St Ste 100

8.80 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4700 Genesee St Ste 100
Cheektowaga, NY 14225

4181 Transit Road, Transit Town Plaza

8.90 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-3:00 PM • Sat: 6:00 AM-10:00 AM✓ Walk-in with Electronic Donor Pass
4181 Transit Road, Transit Town Plaza
Williamsville, NY 14221

5014 Transit Rd Located Inside Immediate Care

8.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5014 Transit Rd Located Inside Immediate Care
Depew, NY 14043

3045 Southwestern Boulevard,

9.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3045 Southwestern Boulevard,
Orchard Park, NY 14127

5340 Transit Road,

9.19 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-3:00 PM • Sat: 6:00 AM-10:00 AM✓ Walk-in with Electronic Donor Pass
5340 Transit Road,
Depew, NY 14043

4845 Transit Road,

9.26 mi
🕑 Testing Hours: Mon–Fri: 5:00 AM-12:30 PM✓ Walk-in with Electronic Donor Pass
4845 Transit Road,
Lancaster, NY 14043

4729 Transit Rd

9.31 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4729 Transit Rd
Depew, NY 14043

1360 North Forest Road,

9.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1360 North Forest Road,
Amherst, NY 14221

295 Essjay Rd

9.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
295 Essjay Rd
Williamsville, NY 14221

51 Webster St

9.44 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
51 Webster St
North Tonawanda, NY 14120

6199 Transit Rd

9.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6199 Transit Rd
Depew, NY 14043

43 Niagara St

9.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
43 Niagara St
North Tonawanda, NY 14120

2390 N Forest Rd Ste 2

9.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2390 N Forest Rd Ste 2
Getzville, NY 14068

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 39 DOT drug testing locations to serve you in and around Buffalo, NY.

In and around Buffalo, NY, our drug testing locations offer DOT Drug Tests, Alcohol Tests, Physicals, Random Pool services, Consortium enrollment, and more for Employers, Owner-Operators, and all DOT modes needing 49 CFR Part 40 compliance including FMCSA, FTA, FAA, USCG, FRA, and PHMSA, complete with same-day service options. Convenient testing centers are close to home or work.

Choose the required DOT service or drug/alcohol test and a convenient testing site nearby. Whether for personal use, employees, or others, our streamlined process makes scheduling DOT drug testing near Buffalo quick and hassle-free.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Buffalo, New York DOT Services

DOT Drug Testing & Services Available in Buffalo, NY:

Accredited Drug Testing, located in Buffalo, NY, specializes in providing DOT services for employers and operators requiring compliance with federal standards, ensuring efficient, thorough testing processes.

DOT Services in Buffalo, NY

DOT regulations require stringent drug and alcohol testing, ensuring employee safety nationwide. In Buffalo, NY, Accredited Drug Testing supports adherence to these regulations, offering comprehensive testing and reporting services.

At Accredited Drug Testing in Buffalo, NY, we prioritize safety through adherence to DOT regulations, offering drug and alcohol tests required by the DOT for all transportation modes, ensuring compliance and safety.

Employers in Buffalo, New York, can rely on us for DOT-compliant services that include drug/alcohol testing, ensuring their workforce meets all regulatory standards for safe, reliable operations.

In Buffalo, NY, Accredited Drug Testing helps security and compliance with DOT regulations through streamlined testing processes designed to meet every criterion essential for transportation industry standards.

FMCSA Information

Accredited Drug Testing in Buffalo, NY, ensures FMCSA compliance with mandatory drug and alcohol testing, crucial for maintaining safety and legal standards in commercial transportation operations.

FMCSA-regulated employers in Buffalo, NY, can trust Accredited Drug Testing for precise, compliant drug screening services, safeguarding driver qualification and public safety.

USCG Information

Accredited Drug Testing in Buffalo, NY offers USCG-compliant testing, ensuring maritime employee standards are met for safety and operational reliability.

Through strategic testing, Accredited Drug Testing ensures Buffalo, NY marine personnel meet USCG drug testing requirements, maintaining essential safety standards.

FAA Information

For Buffalo, NY, Accredited Drug Testing provides FAA-compliant drug screening, essential for ensuring pilot and crew safety and upholding regulatory standards in the aviation sector.

With a focus on aviation safety, Accredited Drug Testing in Buffalo, NY, offers precise FAA drug testing services for compliance and safety in flight operations.

FRA Information

Accredited Drug Testing offers FRA-compliant drug screening in Buffalo, NY, ensuring railway workers meet strict federal safety standards, crucial for the integrity of rail operations.

In Buffalo, NY, FRA-regulated employers rely on Accredited Drug Testing for comprehensive drug testing services essential for railway operational safety and compliance.

FTA Information

Accredited Drug Testing in Buffalo, NY ensures FTA compliance, providing necessary drug and alcohol testing for public transit workers to maintain safety standards and regulatory adherence.

Public transportation in Buffalo, NY, benefits from FTA-compliant testing services from Accredited Drug Testing, ensuring workforce reliability and public safety.

PHMSA Information

Testing for PHMSA compliance is critical, and Accredited Drug Testing in Buffalo, NY, ensures your personnel adhere to federal safety standards through rigorous substance testing protocols.

In Buffalo, NY, Accredited Drug Testing addresses PHMSA requirements, providing specialized drug and alcohol testing services to ensure operational safety and compliance.

Scheduling in Buffalo, NY

To arrange any DOT drug or alcohol test in Buffalo, NY, contact our local team at (800) 221-4291 or utilize our online express system. Select your test and fill out the Donor Information/Registration Section before visiting the testing site.

Use your zip code to find a nearby Buffalo DOT testing center that offers your needed test. Submit and print the donor pass sent to your email or display it on your smartphone at the facility. Registration and payment must be completed in advance, though no appointment is usually needed.

Our SAMHSA-accredited labs ensure all results are confirmed by our certified Medical Review Officers (MROs).

Beyond DOT drug and alcohol testing in Buffalo, Accredited Drug Testing offers locations throughout nearby metropolitan areas and towns.

Whether traveling or at home, rest easy with Accredited Drug Testing. With test centers nationwide, scheduling is simple anytime, anywhere!

New York Dept. of Transportation Updates

Buffalo, NY DUI Statistics

In Buffalo, New York, part of Erie County, DUI (Driving Under the Influence) incidents remain a significant concern for the New York State Department of Transportation (NYSDOT). The city has seen efforts to mitigate DUI cases through stricter law enforcement and community education. DUI statistics show a persistent pattern, primarily involving alcohol. In New York State, stringent penalties are in place, including license suspension, fines, and mandatory education programs. Public awareness campaigns have played a crucial role in reducing DUI incidents. However, Buffalo, NY still faces challenges in completely eliminating driving under the influence, which contributes to road safety issues.

Drug-Involved Accidents in Buffalo, NY

Drug-involved accidents in Buffalo, NY, particularly within Erie County, present a serious concern for traffic safety. The NY State Department of Transportation (NYSDOT) emphasizes the need for proactive measures to address this issue. In New York, drug-related accidents often involve substances like prescription medications, stimulants, and narcotics. The incident rate suggests a need for increased law enforcement presence and comprehensive drug education. New York State has implemented programs to support individuals battling addiction, aiming to reduce the occurrence of drug-involved accidents. Despite these efforts, Buffalo continues to grapple with the impact of drugs on road safety, urging continuous policy evaluation and amendment.

Marijuana-Related Accidents in Buffalo, NY

Since the legalization of recreational marijuana in New York in 2021, the city of Buffalo, within Erie County, has witnessed a change in marijuana-related accident patterns. The NY State Department of Transportation (NYSDOT), alongside local agencies, tracks these incidents to understand their impact on road safety. Educating drivers on the risks of driving under the influence of marijuana is a priority, with campaigns highlighting its impairing effects. While initial fears of a surge in incidents were quantified, a slight rise was recorded but not as significant as anticipated. Continued research and adjustments in law enforcement strategies are underway to address marijuana's role in accident causation.

Buffalo Drug Testing

Quickly find trusted local drug testing centers in Buffalo, NY — fast, convenient, and reliable every time!

Buffalo DOT Drug Testing

Quickly find a local DOT drug testing center in Buffalo, NY — fast, reliable, convenient nationwide service!

Buffalo DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Buffalo, NY.

Buffalo Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Buffalo, NY.v

Buffalo Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Buffalo, NY locations—results you can trust, every time!

Buffalo Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Buffalo, NY.

Buffalo Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Buffalo, NY.

Buffalo 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Buffalo, NY employers and individuals nationwide.

Buffalo 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Buffalo, NY workplace and personal testing needs.

Buffalo On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Buffalo, NY workplace compliant.

Buffalo DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Buffalo, NY drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291