DOT Drug Testing - Middletown, OH

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 40 testing centers in the Middletown area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

40 DOT Drug-Testing Centers in Middletown

Minutes Away, Test Today

1801 Crawford St

0.91 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 07:30 AM - 11:00 AM, 01:00 PM - 03:30 PM
1801 Crawford St
Middletown, OH 45044

4220 Grand Ave

1.28 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:30 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:30 AM, 01:00 PM - 03:00 PM
4220 Grand Ave
Middletown, OH 45044

62 N Breiel Blvd

1.35 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:00 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 07:00 AM - 11:00 AM, 01:00 PM - 03:00 PM
62 N Breiel Blvd
Middletown, OH 45042

3290 Village Dr

2.96 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 07:30 AM - 04:00 PM
3290 Village Dr
Franklin, OH 45005

333 Conover Dr

6.11 mi
🕑 Facility: Mon–Fri: 07:30 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 12:00 PM, 02:00 PM - 04:00 PM
333 Conover Dr
Franklin, OH 45005

540 W Central Ave

7.98 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:00 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 07:30 AM - 11:00 AM, 01:00 PM - 03:00 PM
540 W Central Ave
Springboro, OH 45066

4125 Hamilton Middletown Rd

8.63 mi
🕑 Facility: Mon–Fri: 08:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 10:00 AM - 12:30 PM, 02:00 PM - 04:30 PM
4125 Hamilton Middletown Rd
Hamilton, OH 45011

7324 Yankee Rd Ste B

9.22 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM (Lunch: 12:00 PM - 12:30 PM)⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:30 AM, 12:30 PM - 03:30 PM
7324 Yankee Rd Ste B
Liberty Township, OH 45044

986 Belvedere Dr B

9.95 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:00 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 07:30 AM - 11:00 AM, 01:00 PM - 03:00 PM
986 Belvedere Dr B
Lebanon, OH 45036

6158 Glennsbury Ct

10.18 mi
🕑 Facility: Mon–Fri: 07:30 AM - 03:30 PM (Lunch: 12:00 PM - 12:30 PM)⚡ Drug Screens: Mon–Fri: 07:30 AM - 11:00 AM, 12:30 PM - 02:30 PM
6158 Glennsbury Ct
West Chester, OH 45069

7345 Kingsgate Way

10.87 mi
🕑 Facility: Mon–Fri: 07:30 AM - 03:30 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 02:30 PM
7345 Kingsgate Way
Westchester, OH 45069

1248 Columbus Ave Ste 10

11.31 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Tuesday: 08:00 am - 04:00 pm; Wednesday: 08:00 am - 04:00 pm; Thursday: 08:00 am - 04:00 pm; Friday: 08:00 am - 04:00 pm
1248 Columbus Ave Ste 10
Lebanon, OH 45036

721 Reading Rd

11.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
721 Reading Rd
Mason, OH 45040

8216 Princeton Glendale Rd 144

11.42 mi
🕑 Facility: Mon–Fri: 06:30 AM - 05:00 PM⚡ Drug Screens: Saturday: 07:30 am - 10:00 am
8216 Princeton Glendale Rd 144
West Chester, OH 45069

415 Byers Rd Ste 200

11.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
415 Byers Rd Ste 200
Miamisburg, OH 45342

8210 Springboro Pike

11.69 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 12:00 PM, 01:30 PM - 04:00 PM
8210 Springboro Pike
Miamisburg, OH 45342

1010 Cereal Ave Ste 100

12.20 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 07:30 AM - 04:00 PM
1010 Cereal Ave Ste 100
Hamilton, OH 45013

630 Eaton Ave

12.23 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 10:00 AM - 12:30 PM, 02:00 PM - 03:30 PM
630 Eaton Ave
Hamilton, OH 45013

100 Arrow Springs Blvd Ste 1200

12.31 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 12:00 PM, 01:30 PM - 04:00 PM
100 Arrow Springs Blvd Ste 1200
Lebanon, OH 45036

903 Nw Washington Blvd, Ste C

12.54 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
903 Nw Washington Blvd, Ste C
Hamilton, OH 45013

1 Elizabeth Pl Ste 100

12.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1 Elizabeth Pl Ste 100
Dayton, OH 45417

4201 Aero Dr

12.65 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM (Lunch: 12:00 PM - 12:30 PM)⚡ Drug Screens: Mon–Fri: 08:00 AM - 02:30 PM
4201 Aero Dr
Mason, OH 45040

7450 Mason Montgomery Rd

12.76 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM (Lunch: 12:00 PM - 12:30 PM)⚡ Drug Screens: Mon–Fri: 08:00 AM - 03:30 PM
7450 Mason Montgomery Rd
Mason, OH 45040

7450 Mason - Montgomery Road

12.76 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 08:00 AM - 04:30 PM, 01:30 PM - 04:30 PM
7450 Mason - Montgomery Road
Mason, OH 45040

7901 Schatz Pointe Dr

12.87 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
7901 Schatz Pointe Dr
Centerville, OH 45459

8500 Bilstein Blvd

13.57 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 07:30 AM - 12:30 PM, 02:00 PM - 03:30 PM
8500 Bilstein Blvd
Hamilton, OH 45015

6625 Centerville Business Park

14.13 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6625 Centerville Business Park
Centerville, OH 45459

4362 Mulhauser Rd

14.38 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 08:30 AM - 11:00 AM, 02:00 PM - 03:30 PM
4362 Mulhauser Rd
Fairfield, OH 45014

6592 Sosna Dr

14.50 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 07:30 AM - 04:00 PM
6592 Sosna Dr
Fairfield, OH 45014

9313 S Mason Montgomery Rd, Suite 130

14.61 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
9313 S Mason Montgomery Rd, Suite 130
Mason, OH 45040

780 Nilles Rd B

15.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
780 Nilles Rd B
Fairfield, OH 45014

3801 Hauck Rd

15.23 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 07:30 AM - 11:30 AM, 01:30 PM - 03:30 PM
3801 Hauck Rd
Cincinnati, OH 45241

4015 Executive Park Dr Ste 115

15.41 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 01:30 PM - 04:00 PM
4015 Executive Park Dr Ste 115
Cincinnati, OH 45241

12029 Sheraton Ln

15.60 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM (Lunch: 12:30 PM - 01:30 PM)⚡ Drug Screens: Mon–Fri: 07:00 AM - 02:30 PM
12029 Sheraton Ln
Cincinnati, OH 45246

Fairfield Executive Center

15.71 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 12:00 PM, 02:00 PM - 03:00 PM
Fairfield Executive Center
Fairfield, OH 45014

2884 E Kemper Rd

15.80 mi
🕑 Facility: Mon–Fri: 07:30 AM - 04:30 PM (Lunch: 12:30 PM - 01:30 PM)⚡ Drug Screens: Mon–Fri: 08:00 AM - 12:00 PM, 01:30 PM - 04:00 PM
2884 E Kemper Rd
Cincinnati, OH 45241

11818 Springfield Pike,

16.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11818 Springfield Pike,
Springdale, OH 45246

2023 Springboro W

16.05 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
2023 Springboro W
Moraine, OH 45439

360 Glensprings Dr

16.09 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:30 PM (Lunch: 11:00 AM - 12:00 PM)⚡ Drug Screens: Mon–Fri: 07:00 AM - 03:30 PM
360 Glensprings Dr
Springdale, OH 45246

1104 Kemper Meadow Dr

16.94 mi
🕑 Facility: Mon–Fri: 07:00 AM - 05:00 PM⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:30 AM, 01:30 PM - 03:30 PM
1104 Kemper Meadow Dr
Cincinnati, OH 45240

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 40 DOT drug testing locations to serve you in and around Middletown, OH.

Our Middletown, OH DOT testing centers offer an array of services including Drug and Alcohol Testing, DOT Physicals, Random Pools, Consortium enrollment, MIS reports, MVR reports, and driver qualification reports. These services comply with 49 CFR Part 40 regulations across FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Conveniently located near your home or workplace, our centers offer prompt, same-day services.

Choose from our variety of DOT services or drug/alcohol tests at a nearby Middletown location. Whether for personal, employee, or third-party testing, our intuitive setup makes arranging DOT drug testing in Middletown simple and efficient.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Middletown, Ohio DOT Services

DOT Drug Testing & Services Available in Middletown, OH:

Accredited Drug Testing proudly organizes DOT services in Middletown, Ohio, offering comprehensive drug and alcohol testing aligned with federal regulations, ensuring industry-wide safety and reliability.

DOT Services in Middletown, OH

Strict DOT drug and alcohol screening regulations are in place to ensure a safe work environment. In Middletown, OH, these rules require thorough compliance checks to maintain public safety.

Employers in Middletown, Ohio, must adhere to DOT protocols involving pre-employment, post-accident, and random testing processes, which are vital for highway safety.

Ensuring safety, DOT standards in Middletown, OH, cover all transport modes, where continuous monitoring and regular assessments of employees are employed.

In Middletown, Ohio, DOT regulation compliance guarantees the well-being of both employees and the wider public, discouraging substance misuse among transportation workers.

FMCSA Information

FMCSA regulations in Middletown, OH, enforce stringent drug and alcohol protocols to ensure drivers operate safely without impairment.

Trucking companies in Middletown, OH, must adhere to FMCSA random testing mandates, enhancing road safety measures.

USCG Information

For USCG regulations in Middletown, Ohio, marine operators are frequently tested to prevent substance-related maritime incidents.

In Middletown, OH, USCG policies enforce rigorous drug and alcohol checks across boating personnel to uphold waterway safety.

FAA Information

FAA mandates in Middletown, OH, stipulate stringent drug and alcohol testing for aviation personnel to uphold sky safety.

Airlines in Middletown, Ohio, enforce FAA regulations through random and routine testing, safeguarding against flight risks.

FRA Information

FRA rules encompass comprehensive testing series in Middletown, OH, prioritizing safety within rail yards and operations.

Middletown, Ohio's rail operators undergo mandatory FRA drug checks, combating risk factors inherent to rail transport.

FTA Information

FTA testing protocols in Middletown, OH, help maintain passenger and crew safety via systematic drug screening.

Middletown, Ohio transit workers are subjected to FTA-assigned random testing to promote commuter trust.

PHMSA Information

PHMSA guidelines in Middletown, OH, target drug and alcohol misuse prevention in the pipeline sector, promoting a safety-centric approach.

Workers in Middletown, OH, engage in mandatory PHMSA testing, ensuring pipelines operate under substance-free conditions.

Scheduling in Middletown, OH

To initiate a DOT drug or alcohol test in Middletown, OH, contact our scheduling team at (800) 221-4291 or register online through our express system. Be sure to complete the Donor Information/Registration section ahead of visiting the testing center.

The zip code entered defines the nearest precinct for DOT drug or alcohol testing in Middletown, Ohio. You'll receive a donor pass with location details and instructions via email. Most tests don't require appointments, but payment and completion of donor details are needed upon registration.

Our SAMHSA-accredited labs ensure all results pass through our in-house licensed Medical Review Officers (MRO) for authoritative oversight.

Beyond Middletown, OH, Accredited Drug Testing operates across the broader metro area and neighboring locales, offering a comprehensive network for testing.

Whether traveling or on holiday, relax—Accredited Drug Testing operates extensive testing locations nationwide, promising ease in scheduling tests anywhere, any time!

Ohio Dept. of Transportation Updates

DUI Statistics in Middletown, OH

Middletown, Ohio, located in Butler County, has seen a notable number of DUI incidents over recent years. Ohio's Department of Transportation (ODOT) provides data indicating that DUI offenses significantly contribute to road safety concerns in this region. Increased law enforcement efforts have been aimed at reducing these numbers, with initiatives such as sobriety checkpoints and public education campaigns about the dangers of drunk driving. Despite these efforts, Middletown, OH continues to face challenges with alcohol-impaired driving, which has led to numerous accidents, posing safety risks to drivers and pedestrians. The community continues to work collaboratively with state and local agencies to implement prevention strategies and support programs to address and mitigate DUI incidents effectively.

Drug-Involved Accidents in Middletown, OH

Drug-involved accidents have become a growing concern in Middletown, Ohio, and Butler County. According to the Ohio Department of Transportation, the number of accidents involving drivers impaired by drugs has shown an upward trend. Various substances, including prescription medications and illegal drugs, have been contributing factors. This rise in drug-related incidents has prompted local authorities in Middletown, OH to increase their focus on identifying and apprehending drug-impaired drivers through enhanced training for officers and collaboration with health organizations. Community awareness programs are also being implemented to educate the public on the risks associated with drug-impaired driving in efforts to reduce the incidence of such accidents and improve road safety across the region.

Marijuana-Related Accidents in Middletown, OH

The legalization of marijuana in several states has prompted discussions in Middletown, Ohio, regarding its impact on road safety. While Ohio itself has strict regulations concerning marijuana use and driving, Butler County has observed a number of accidents where marijuana use was a contributing factor. Data from the Ohio Department of Transportation suggests that marijuana, while perhaps less impactful than alcohol when used alone, still poses a risk when drivers operate vehicles under its influence. In Middletown, OH, local law enforcement agencies have begun to implement specialized training programs to better equip officers to detect marijuana impairment. Community efforts continue to focus on educating residents about the dangers of driving under the influence of marijuana to prevent accidents and ensure safer roads.

Middletown Drug Testing

Quickly find trusted local drug testing centers in Middletown, OH — fast, convenient, and reliable every time!

Middletown DOT Drug Testing

Quickly find a local DOT drug testing center in Middletown, OH — fast, reliable, convenient nationwide service!

Middletown DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Middletown, OH.

Middletown Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Middletown, OH.v

Middletown Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Middletown, OH locations—results you can trust, every time!

Middletown Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Middletown, OH.

Middletown Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Middletown, OH.

Middletown 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Middletown, OH employers and individuals nationwide.

Middletown 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Middletown, OH workplace and personal testing needs.

Middletown On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Middletown, OH workplace compliant.

Middletown DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Middletown, OH drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291