DOT Drug Testing - Middletown, VA

FMCSA-USCG-FTA-FRA-FAA-PHMSA

With 37 testing centers in the Middletown area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

37 DOT Drug-Testing Centers in Middletown

Minutes Away, Test Today

842 N Shenandoah Ave

8.24 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
842 N Shenandoah Ave
Front Royal, VA 22630

120 N Commerce Ave Ste 102

9.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
120 N Commerce Ave Ste 102
Front Royal, VA 22630

2505 Valley Ave

10.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2505 Valley Ave
Winchester, VA 22601

110 Featherbed Ln Ste 6

11.05 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
110 Featherbed Ln Ste 6
Winchester, VA 22601

607 E Jubal Early Dr

11.20 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
607 E Jubal Early Dr
Winchester, VA 22601

1840 Amherst St

12.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1840 Amherst St
Winchester, VA 22601

1867 Amherst St

12.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1867 Amherst St
Winchester, VA 22601

1724 Amherst St

12.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1724 Amherst St
Winchester, VA 22601

207 Gateway Dr

13.42 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
207 Gateway Dr
Winchester, VA 22603

759 S Main St

16.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
759 S Main St
Woodstock, VA 22664

200 Memorial Dr

27.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
200 Memorial Dr
Luray, VA 22835

300 Preston St

29.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
300 Preston St
Charles Town, WV 25414

300 S Preston St

29.13 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
300 S Preston St
Ranson, WV 25438

84 Somerset Blvd

30.33 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-4:15 PM✓ Walk-in with Electronic Donor Pass
84 Somerset Blvd
Charles Town, WV 25414

363 Sunrise Blvd

30.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
363 Sunrise Blvd
Romney, WV 26757

75 W Lee Hwy

33.18 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
75 W Lee Hwy
Warrenton, VA 20186

500 Hospital Dr

33.58 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
500 Hospital Dr
Warrenton, VA 20186

493 Blackwell Rd Ste 202

33.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
493 Blackwell Rd Ste 202
Warrenton, VA 20186

170 W Shirley Ave

33.76 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 03:00 PM
170 W Shirley Ave
Warrenton, VA 20186

651 Foxcroft Ave

33.77 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
651 Foxcroft Ave
Martinsburg, WV 25401

83 Retail Commons Pkwy

33.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
83 Retail Commons Pkwy
Martinsburg, WV 25403

315 Rock Cliff Dr,

34.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
315 Rock Cliff Dr,
Martinsburg, WV 25401

1355 Edwin Miller Blvd Ste A

35.58 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1355 Edwin Miller Blvd Ste A
Martinsburg, WV 25404

97 Administrative Dr

35.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
97 Administrative Dr
Martinsburg, WV 25404

261 Berkmore Pl Ste 1a

35.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
261 Berkmore Pl Ste 1a
Berkeley Springs, WV 25411

112 Kuykendall Ln

37.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
112 Kuykendall Ln
Moorefield, WV 26836

319 B Lutz Ave

37.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
319 B Lutz Ave
Martinsburg, WV 25404

6950 Piedmont Center Plz

38.35 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6950 Piedmont Center Plz
Gainesville, VA 20155

211 S King St Suite C,

38.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
211 S King St Suite C,
Leesburg, VA 20175

14370 Lee Highway, Suite 101

39.00 mi
🕑 Testing Hours: Mon–Fri: 10:00 AM-2:00 PM • Sat: 8:30 AM-9:30 AM✓ Walk-in with Electronic Donor Pass
14370 Lee Highway, Suite 101
Gainesville, VA 20155

7588 Gardner Park Dr

39.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7588 Gardner Park Dr
Gainesville, VA 20155

222 Catoctin Cir,se, Ste 100

39.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
222 Catoctin Cir,se, Ste 100
Leesburg, VA 20175

51 Catoctin Cir Ne

39.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
51 Catoctin Cir Ne
Leesburg, VA 20176

952 Edwards Ferry Rd Ne

40.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
952 Edwards Ferry Rd Ne
Leesburg, VA 20176

Onsite Only

40.83 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Onsite Only
Ashburn, VA 20148

302 E Davis St

41.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
302 E Davis St
Culpeper, VA 22701

1420 S Main St

41.83 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM • Sat:,Sun: 8:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
1420 S Main St
Culpeper, VA 22701

DOT Drug Testing, DOT Consortium, DOT Physicals, FMCSA Clearinghouse, Serving All DOT modes

We have 37 DOT drug testing locations to serve you in and around Middletown, VA.

In and around Middletown, VA, our local DOT Drug Testing centers offer comprehensive DOT services including Drug and Alcohol Testing, Physicals, Random pools, Consortium enrollment, and various compliance reports. We cater to employers, single operators, and all DOT modes that must adhere to 49 CFR Part 40, covering FMCSA, FTA, FAA, USCG, FRA, and PHMSA. Our centers are conveniently located, providing swift, same-day service in most situations.

Select the DOT service or drug/alcohol test you need, choose a nearby location, and get tested easily whether it's for you, your employees, or another person. Our process is streamlined to allow effortless scheduling for DOT drug testing in Middletown.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

Middletown, Virginia DOT Services

DOT Drug Testing & Services Available in Middletown, VA:

Accredited Drug Testing's DOT services in Middletown, VA offer comprehensive screening processes for diverse needs. Our facilities provide the necessary tests to comply with DOT regulations, ensuring safety and compliance for individuals and companies.

DOT Services in Middletown, VA

DOT regulations mandate stringent drug and alcohol screening to bolster employee safety. Employers in industries governed by DOT in Middletown, Virginia must ensure compliance with rules for a safer work environment.

The regulations require pre-employment, random, post-accident, reasonable suspicion, and return-to-duty testing. In Middletown, VA, this helps in maintaining a drug-free workplace, protecting both workers and the community.

Employee safety is a top priority under DOT's standards. In Middletown, Virginia, regulations ensure safety-sensitive employees receive adequate training and testing to prevent substance-related incidents.

Employers in Middletown must adhere to DOT screening policies, focusing on key sectors like transportation. Complying with these rules ensures safety and operational integrity within the industry.

FMCSA Information

In Middletown, VA, FMCSA's rules mandate drug and alcohol testing for safety-sensitive positions, strengthening the safety of the roadways.

FMCSA regulations ensure that drivers in Middletown, Virginia undergo regular testing to uphold safe driving standards.

USCG Information

In Middletown, VA, USCG regulations enforce periodic testing for maritime personnel, ensuring smooth and secure waterways.

Middletown, Virginia complies with USCG rules, enhancing maritime safety with rigorous employee testing programs.

FAA Information

FAA rules in Middletown, VA involve stringent periodic testing to ensure aviation safety and personnel compliance with drug and alcohol guidelines.

Routine testing as per FAA standards in Middletown, VA ensures high aviation safety and compliance levels for air personnel.

FRA Information

FRA mandates in Middletown, VA ensure secure train operations remain on course through regular employee testing.

Regular test compliance under FRA guidelines maintains consistent train safety in Middletown, Virginia.

FTA Information

FTA's testing requirements in Middletown, VA secure public transit systems by enforcing regular employee screenings.

Keeping Middletown, Virginia transit safe involves adhering to FTA's comprehensive testing protocols.

PHMSA Information

PHMSA’s drug testing rules in Middletown, VA are critical for maintaining safety in the handling of hazardous materials.

Employees in Middletown, Virginia dealing with hazardous materials are tested regularly as per PHMSA guidelines.

Scheduling in Middletown, VA

To arrange a DOT drug or alcohol test in Middletown, VA, contact us at (800) 221-4291 or schedule online through our express system. Select your test, fill in the Donor Information, and ensure registration before heading to the center.

Your entered zip code identifies the nearest DOT testing facility. After selecting, you'll receive a donor pass via email with location details. Bring this pass or display it on your phone. Appointments aren't necessary generally, but complete donor information and payment are needed at registration.

Our labs, certified by SAMHSA, ensure verified results through our Medical Review Officers (MRO), providing trusted services.

Alongside DOT testing in Middletown, VA, we offer additional centers throughout the metropolitan and neighboring areas, ensuring accessibility.

Whether traveling or on holiday, Accredited Drug Testing provides nationwide testing facility access, facilitating test scheduling at any time, anywhere.

Virginia Dept. of Transportation Updates

DUI Statistics in Middletown, VA

Middletown, VA, located in the scenic region of Frederick County, has seen a notable number of DUI incidents over recent years. In Virginia, DUI incidents are taken seriously as they directly impact public safety and have significant legal consequences. In Frederick County, which Middletown is a part of, law enforcement agencies are particularly vigilant. In the past year, the area reported a considerable number of DUI arrests, reflecting both the challenges faced by the community and the aggressive enforcement of DUI laws. Virginia's approach to DUI is stringent, with penalties including license suspension, fines, and possible jail time. The focus on prevention through community programs aims to reduce these incidents in future, emphasizing the importance of educating drivers about the risks and legal repercussions of driving under the influence.

Drug-Involved Accidents in Frederick County, VA

Frederick County, encompassing the town of Middletown, VA, has experienced challenges related to drug-involved accidents. The intersection of highways and local roads in this part of Virginia sees regular traffic, making the vigilance against drug-influenced driving crucial. Recent statistics highlight that a percentage of traffic accidents in this area involved drivers under the influence of illegal substances. The Virginia Department of Transportation (VDOT) and local enforcement agencies stress the importance of drug education and stringent enforcement of laws to curb this trend. Efforts include regular road checkpoints and community outreach programs aimed at educating drivers about the perils of operating vehicles under the influence of drugs, contributing to overall safety within the state.

Marijuana-Related Accidents in Middletown, VA

In the town of Middletown, VA, and the broader Frederick County area, the discussion around marijuana-related accidents has become increasingly relevant. With changes in marijuana legislation in Virginia, law enforcement and community safety programs have adapted to new challenges. Although marijuana usage is regulated, there has been an observable impact on driving safety in Middletown due to impaired driving incidents involving marijuana. The Virginia Department of Motor Vehicles (DMV) and local police have initiated campaigns to raise awareness about the impairing effects of marijuana on driving. These efforts are critical in addressing roadway safety and have included increased patrols and public service announcements to reduce accidents associated with marijuana usage while driving.

Middletown Drug Testing

Quickly find trusted local drug testing centers in Middletown, VA — fast, convenient, and reliable every time!

Middletown DOT Drug Testing

Quickly find a local DOT drug testing center in Middletown, VA — fast, reliable, convenient nationwide service!

Middletown DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Middletown, VA.

Middletown Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Middletown, VA.v

Middletown Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Middletown, VA locations—results you can trust, every time!

Middletown Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Middletown, VA.

Middletown Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Middletown, VA.

Middletown 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Middletown, VA employers and individuals nationwide.

Middletown 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Middletown, VA workplace and personal testing needs.

Middletown On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Middletown, VA workplace compliant.

Middletown DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Middletown, VA drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT drug test is a federally regulated drug test required for safety-sensitive transportation employees, such as CDL drivers. It is collected and processed under specific DOT procedures (49 CFR Part 40), including strict chain of custody, certified lab analysis, and documented results appropriate for DOT compliance."
DOT testing applies to employees in safety-sensitive roles, including commercial drivers and other regulated transportation workers. Employers must ensure pre-employment testing, random testing, post-accident testing, reasonable suspicion testing, return-to-duty, and follow-up testing are performed as required."
Yes. Accredited Drug Testing maintains access to DOT-compliant collection sites nationwide. Drivers and employers can schedule testing at a nearby location for required DOT drug and alcohol tests, including urgent post-accident or reasonable suspicion testing."
DOT-required testing includes pre-employment, random, post-accident, reasonable suspicion/reasonable cause, return-to-duty, and follow-up testing. For alcohol, evidential breath testing devices are used to document alcohol concentration following DOT protocol."
Yes. Solo CDL drivers and owner-operators are still subject to DOT testing requirements. They must participate in a compliant random testing program and complete required testing events using DOT-approved processes. Accredited Drug Testing assists in meeting these obligations."
If a DOT drug test screen is not negative, the specimen is sent for confirmatory testing using more specific laboratory methods. Final results are documented and reported according to DOT rules. An employee who has a violation must complete the DOT return-to-duty and follow-up process before resuming safety-sensitive work."
Yes. DOT drug and alcohol test results are released only to authorized employer representatives and are maintained in compliance files for DOT purposes. Results are handled in a controlled and confidential manner."
Yes. Accredited Drug Testing supports urgent post-accident and reasonable suspicion testing because DOT rules require timely documentation of possible impairment following an incident or observed behavior in a safety-sensitive environment."

Customer Reviews

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Super Easy to Schedule

Super easy to schedule and get what you need, weather for a job, personal, or court ordered! I believe I spoke with Justine who was helpful in answering all my questions and stayed on the phone with me until she was sure I was all set and got the emails I needed.

Mandy Ryan - 12/14/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291