✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Middletown, VA

With 37 testing centers in the Middletown area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

37 Drug-Testing Centers in Middletown

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

842 N Shenandoah Ave

8.24 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
842 N Shenandoah Ave
Front Royal, VA 22630

120 N Commerce Ave Ste 102

9.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
120 N Commerce Ave Ste 102
Front Royal, VA 22630

2505 Valley Ave

10.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2505 Valley Ave
Winchester, VA 22601

110 Featherbed Ln Ste 6

11.05 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
110 Featherbed Ln Ste 6
Winchester, VA 22601

607 E Jubal Early Dr

11.20 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
607 E Jubal Early Dr
Winchester, VA 22601

1840 Amherst St

12.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1840 Amherst St
Winchester, VA 22601

1867 Amherst St

12.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1867 Amherst St
Winchester, VA 22601

1724 Amherst St

12.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1724 Amherst St
Winchester, VA 22601

207 Gateway Dr

13.42 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
207 Gateway Dr
Winchester, VA 22603

759 S Main St

16.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
759 S Main St
Woodstock, VA 22664

200 Memorial Dr

27.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
200 Memorial Dr
Luray, VA 22835

300 Preston St

29.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
300 Preston St
Charles Town, WV 25414

300 S Preston St

29.13 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
300 S Preston St
Ranson, WV 25438

84 Somerset Blvd

30.33 mi
🕑 Testing Hours: Mon–Fri: 8:30 AM-4:15 PM✓ Walk-in with Electronic Donor Pass
84 Somerset Blvd
Charles Town, WV 25414

363 Sunrise Blvd

30.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
363 Sunrise Blvd
Romney, WV 26757

75 W Lee Hwy

33.18 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
75 W Lee Hwy
Warrenton, VA 20186

500 Hospital Dr

33.58 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
500 Hospital Dr
Warrenton, VA 20186

493 Blackwell Rd Ste 202

33.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
493 Blackwell Rd Ste 202
Warrenton, VA 20186

170 W Shirley Ave

33.76 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:00 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 03:00 PM
170 W Shirley Ave
Warrenton, VA 20186

651 Foxcroft Ave

33.77 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
651 Foxcroft Ave
Martinsburg, WV 25401

83 Retail Commons Pkwy

33.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
83 Retail Commons Pkwy
Martinsburg, WV 25403

315 Rock Cliff Dr,

34.82 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
315 Rock Cliff Dr,
Martinsburg, WV 25401

1355 Edwin Miller Blvd Ste A

35.58 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1355 Edwin Miller Blvd Ste A
Martinsburg, WV 25404

97 Administrative Dr

35.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
97 Administrative Dr
Martinsburg, WV 25404

261 Berkmore Pl Ste 1a

35.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
261 Berkmore Pl Ste 1a
Berkeley Springs, WV 25411

112 Kuykendall Ln

37.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
112 Kuykendall Ln
Moorefield, WV 26836

319 B Lutz Ave

37.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
319 B Lutz Ave
Martinsburg, WV 25404

6950 Piedmont Center Plz

38.35 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6950 Piedmont Center Plz
Gainesville, VA 20155

211 S King St Suite C,

38.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
211 S King St Suite C,
Leesburg, VA 20175

14370 Lee Highway, Suite 101

39.00 mi
🕑 Testing Hours: Mon–Fri: 10:00 AM-2:00 PM • Sat: 8:30 AM-9:30 AM✓ Walk-in with Electronic Donor Pass
14370 Lee Highway, Suite 101
Gainesville, VA 20155

7588 Gardner Park Dr

39.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7588 Gardner Park Dr
Gainesville, VA 20155

222 Catoctin Cir,se, Ste 100

39.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
222 Catoctin Cir,se, Ste 100
Leesburg, VA 20175

51 Catoctin Cir Ne

39.34 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
51 Catoctin Cir Ne
Leesburg, VA 20176

952 Edwards Ferry Rd Ne

40.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
952 Edwards Ferry Rd Ne
Leesburg, VA 20176

Onsite Only

40.83 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Onsite Only
Ashburn, VA 20148

302 E Davis St

41.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
302 E Davis St
Culpeper, VA 22701

1420 S Main St

41.83 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM • Sat:,Sun: 8:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
1420 S Main St
Culpeper, VA 22701

Employment, DOT, Court Ordered, Probation, Family

Accredited Drug Testing provides an extensive array of drug and alcohol testing options at our 37 testing facilities in the Middletown, Virginia area. Whether you require DOT or non-DOT urine analysis, breath alcohol evaluations, EtG alcohol tests, or hair follicle drug tests, we cater to individuals, businesses, and legal purposes. Our rapid testing and SAMSA certified lab evaluations demand only a short drive or walk from most Middletown residences or workplaces. We also deliver Occupational Health Assessments, Clinical Testing, and Background Verification Services.

To initiate your test, dial (800) 221-4291 or register online. Pick your desired test and find a center nearby, whether it's for you, your staff, or a third party. Scheduling is swift and straightforward—contact our scheduling team or book your test online at any time. Our efficient and intuitive system makes drug testing arrangements in Middletown hassle-free and convenient.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Middletown, VA

1

Order Online or Call

Select your test & search nearby Middletown collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Middletown, VA Drug Testing

Drug Testing Services Available at Our Middletown, VA Labs:

At our Middletown drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Virginia Drug Testing Laws & Compliance in Middletown

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Middletown, VA adhering strictly to Va. Code § 65.2-306, federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
Va. Code § 65.2-306
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Legal
Workplace Rights
Zero-Tolerance Protected

Virginia Code § 65.2-306 supports workplace drug safety, denying workers compensation claims when post-accident testing confirms illegal drug or alcohol influence.

Under Va. Code § 65.2-306, employers in Middletown may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Legal in Virginia), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Middletown, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Middletown and Virginia.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Middletown, VA

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Middletown to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Middletown Neighborhoods Covered:

● Downtown Middletown ● Central Business District ● North Middletown Commercial Corridor ● South Middletown Industrial Park ● East Middletown Metro Area ● West Middletown Trade Center ● Medical & Healthcare District ● Airport / Logistics Corridor

Major Employment & Commercial Hubs Served:

Middletown Main Street Commercial District • Middletown Regional Trade Center • County Industrial Parkway • Metro Healthcare & Occupational Medical Corridor

Located anywhere in Middletown? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Middletown

Stephens City • Lebanon Church • Strasburg • Mccoy • Riverton • Shenandoah Shores • Front Royal • Toms Brook

Middletown, VA Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Middletown.

Test Type & Local Panel Detection Window Common Use Cases in Middletown Turnaround Time
5-Panel Urine Screen in Middletown →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Middletown →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Middletown →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Middletown →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Middletown →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Middletown →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Middletown, VA

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Middletown.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Middletown facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Middletown, VA

Need immediate walk-in service in Middletown? View our Middletown, VA Drug Testing Facility Guide or order an instant electronic donor pass online.

Middletown Drug Testing

Quickly find trusted local drug testing centers in Middletown, VA — fast, convenient, and reliable every time!

Middletown DOT Drug Testing

Quickly find a local DOT drug testing center in Middletown, VA — fast, reliable, convenient nationwide service!

Middletown DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Middletown, VA.

Middletown Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Middletown, VA.v

Middletown Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Middletown, VA locations—results you can trust, every time!

Middletown Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Middletown, VA.

Middletown Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Middletown, VA.

Middletown 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Middletown, VA employers and individuals nationwide.

Middletown 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Middletown, VA workplace and personal testing needs.

Middletown On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Middletown, VA workplace compliant.

Middletown DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Middletown, VA drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Virginia, workplace drug testing is governed by statutory guidelines under Va. Code § 65.2-306. Private employers generally operate under a Virginia Code § 65.2-306 and labor rules, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Virginia who implement a certified Drug-Free Workplace Program in compliance with Va. Code § 65.2-306 can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Virginia state law compliance standards, state statutes protects medical cannabis oil cardholders for non-safety roles. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 37 certified collection sites in the Middletown area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Middletown, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

Very professional and very quick

Very professional and very quick service to schedule appointments! I am pleased with the services and would definitely recommend using them for any drug testing needs!

Carrie Feerick - 4/19/2025

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Explained everything so no questions

Torin was great. Explained everything so no questions

Tony Lombardo - 4/18/2025


(800) 221-4291