✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Alafaya, FL

With 35 testing centers in the Alafaya area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

35 Drug-Testing Centers in Alafaya

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

2504 S Alafaya Trl, Ste 160

0.59 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2504 S Alafaya Trl, Ste 160
Orlando, FL 32828

12301 Lake Underhill Rd Ste 118

1.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12301 Lake Underhill Rd Ste 118
Orlando, FL 32828

250 N Alafaya Trl Ste 135

2.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
250 N Alafaya Trl Ste 135
Orlando, FL 32828

12265 Lake Underhill Rd Ste 135

2.76 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12265 Lake Underhill Rd Ste 135
Orlando, FL 32825

11867 E Colonial Dr

3.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11867 E Colonial Dr
Orlando, FL 32826

11250 E Colonial Dr

3.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11250 E Colonial Dr
Orlando, FL 32817

2000 N Alafaya Trail, Suite 100

4.03 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-2:00 PM • Sat: 7:00 AM-11:00 AM✓ Walk-in with Electronic Donor Pass
2000 N Alafaya Trail, Suite 100
Orlando, FL 32826

2200 N Alafaya Trl Ste 900

4.10 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2200 N Alafaya Trl Ste 900
Orlando, FL 32826

509 S Semoran Blvd

8.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
509 S Semoran Blvd
Orlando, FL 32807

36 S Semoran Blvd Ste B

8.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
36 S Semoran Blvd Ste B
Orlando, FL 32807

483 N Semoran Blvd Suite 103

8.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
483 N Semoran Blvd Suite 103
Winter Park, FL 32792

5575 S Semoran Blvd, Suite 501

8.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5575 S Semoran Blvd, Suite 501
Orlando, FL 32822

5425 S Semoran Blvd

8.25 mi
🕑 Facility: Mon–Fri: 06:30 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 06:30 AM - 03:00 PM
5425 S Semoran Blvd
Orlando, FL 32822

5810 S Semoran Blvd

8.40 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
5810 S Semoran Blvd
Orlando, FL 32822

5931 Brick Court Suite 100

8.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5931 Brick Court Suite 100
Winter Park, FL 32792

4122 Metric Dr Ste 800

8.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4122 Metric Dr Ste 800
Winter Park, FL 32792

7480 Aloma Ave

8.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7480 Aloma Ave
Winter Park, FL 32792

7457 Aloma Ave Ste 301

8.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7457 Aloma Ave Ste 301
Winter Park, FL 32792

5931 Aloma Ave Ste 100

9.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5931 Aloma Ave Ste 100
Winter Park, FL 32792

8010 Red Bug

9.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8010 Red Bug
Oviedo, FL 32765

3099 Aloma Ave

9.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3099 Aloma Ave
Winter Park, FL 32792

1950 W Sr 426 Ste 108,

9.68 mi
🕑 Testing Hours: Mon–Fri: 11:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
1950 W Sr 426 Ste 108,
Oviedo, FL 32765

5355 Red Bug Lake Rd

9.90 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
5355 Red Bug Lake Rd
Winter Springs, FL 32708

1410 W Broadway St Ste 108

10.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1410 W Broadway St Ste 108
Oviedo, FL 32765

630 N Bumby Ave

10.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
630 N Bumby Ave
Orlando, FL 32803

813 E Michigan St

11.36 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM • Sat: 8:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
813 E Michigan St
Orlando, FL 32806

709 E Michigan St

11.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
709 E Michigan St
Orlando, FL 32806

701 E Michigan St

11.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
701 E Michigan St
Orlando, FL 32806

Bldg 49635 - Rm 1219

11.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Bldg 49635 - Rm 1219
Orlando, FL 32899

611 N Wymore Rd Ste 220

11.80 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
611 N Wymore Rd Ste 220
Winter Park, FL 32789

601 E Colonial Dr

11.80 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
601 E Colonial Dr
Orlando, FL 32803

4401 S Orange Ave Ste 110

11.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4401 S Orange Ave Ste 110
Orlando, FL 32806

2323 S Orange Ave Ste A

11.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2323 S Orange Ave Ste A
Orlando, FL 32806

3615 S Orange Ave

11.98 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3615 S Orange Ave
Orlando, FL 32806

324 E Par St.., Ste 100,

12.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
324 E Par St.., Ste 100,
Orlando, FL 32804

Employment, DOT, Court Ordered, Probation, Family

At our 35 testing centers in the Alafaya, Florida area, Accredited Drug Testing delivers thorough drug and alcohol testing services. Our offerings include both DOT and non-DOT urine drug tests, breath alcohol tests, EtG alcohol testing, as well as hair drug tests, catering to individuals, employers, and legal requirements. Experience rapid results testing in Alafaya, FL, with SAMSA certified lab analysis, where same-day service is an option and many centers are just a short distance from your home or office. We also provide Occupational Health Testing, Clinical Testing, and Background Checks.

Dial (800) 221-4291 or sign up online. Easily select your preferred test and find a nearby location—services are offered for self, employees, or others. The process of scheduling a test is swift and straightforward, whether you call our scheduling team or use our 24/7 online booking. Our efficient and intuitive process ensures effortless drug testing arrangements near Alafaya.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Alafaya, FL

1

Order Online or Call

Select your test & search nearby Alafaya collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Alafaya, FL Drug Testing

Drug Testing Services Available at Our Alafaya, FL Labs:

At our Alafaya drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Florida Drug Testing Laws & Compliance in Alafaya

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Alafaya, FL adhering strictly to Fla. Stat. § 440.102, federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
Fla. Stat. § 440.102
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Prohibited
Workplace Rights
Zero-Tolerance Protected

Florida Statute 440.102 establishes Drug-Free Workplace Program standards, providing premium discounts on worker compensation insurance for verified testing procedures.

Under Fla. Stat. § 440.102, employers in Alafaya may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Prohibited in Florida), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Alafaya, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Alafaya and Florida.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Alafaya, FL

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Alafaya to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Alafaya Neighborhoods Covered:

● Downtown Alafaya ● Central Business District ● North Alafaya Commercial Corridor ● South Alafaya Industrial Park ● East Alafaya Metro Area ● West Alafaya Trade Center ● Medical & Healthcare District ● Airport / Logistics Corridor

Major Employment & Commercial Hubs Served:

Alafaya Main Street Commercial District • Alafaya Regional Trade Center • County Industrial Parkway • Metro Healthcare & Occupational Medical Corridor

Located anywhere in Alafaya? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Alafaya

Rio Pinar • Bithlo • East Orange • Jay • Wedgefield • Azalea Park • Goldenrod • Chuluota

Alafaya, FL Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Alafaya.

Test Type & Local Panel Detection Window Common Use Cases in Alafaya Turnaround Time
5-Panel Urine Screen in Alafaya →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Alafaya →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Alafaya →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Alafaya →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Alafaya →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Alafaya →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Alafaya, FL

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Alafaya.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Alafaya facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Alafaya, FL

Need immediate walk-in service in Alafaya? View our Walk-In Drug Testing Near Me in Alafaya, FL facility guide or order an instant electronic donor pass online.

Alafaya Drug Testing

Quickly find trusted local drug testing centers in Alafaya, FL — fast, convenient, and reliable every time!

Alafaya DOT Drug Testing

Quickly find a local DOT drug testing center in Alafaya, FL — fast, reliable, convenient nationwide service!

Alafaya DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Alafaya, FL.

Alafaya Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Alafaya, FL.v

Alafaya Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Alafaya, FL locations—results you can trust, every time!

Alafaya Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Alafaya, FL.

Alafaya Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Alafaya, FL.

Alafaya 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Alafaya, FL employers and individuals nationwide.

Alafaya 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Alafaya, FL workplace and personal testing needs.

Alafaya On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Alafaya, FL workplace compliant.

Alafaya DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Alafaya, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Florida, workplace drug testing is governed by statutory guidelines under Fla. Stat. § 440.102. Private employers generally operate under a Drug-Free Workplace Program under Fla. Stat. § 440.102, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Florida who implement a certified Drug-Free Workplace Program in compliance with Fla. Stat. § 440.102 can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Florida state law compliance standards, state statutes does not require accommodation of medical marijuana in workplace. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 35 certified collection sites in the Alafaya area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Alafaya, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

I love the customer service

I love the customer service. The lady was patient enough to help me book my appointment with ease and ensuring I get the location that's best for me. Keep up the good work!

Elizabeth Chiaka - 9/19/2024

Excellant Performance

The visit here is always the best . The place is always really clean. The employees are super courteous, very polite, and professional. This is the only drug lab I like to go do my drug and alcohol test. I would like to tell them thank you so much for thier excellent performance and job

Eli Gonzalez - 1/4/2025

Accredited has been wonderful to work with!

Accredited has been wonderful to work with! My court ordered tests are on a very strange schedule but every time I call, they are quick to schedule me and are always able to find a location that works for where I’m at and when! Thank you for making this rather undesirable situation less stressful and efficient.

Madison Sanderford - 8/19/2024


(800) 221-4291