✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Chicago, IL

With 33 testing centers in the Chicago area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

33 Drug-Testing Centers in Chicago

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

2361 Reliable Parkway

0.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2361 Reliable Parkway
Chicago, IL 60686

2393 Reliable Parkway

0.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2393 Reliable Parkway
Chicago, IL 60686

3145 S Ashland Ave Ste 110

1.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3145 S Ashland Ave Ste 110
Chicago, IL 60608

2907 S Wabash Ave Ste 100

1.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2907 S Wabash Ave Ste 100
Chicago, IL 60616

3201 S Wallace St

1.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3201 S Wallace St
Chicago, IL 60616

3316 S Ashland Ave

1.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3316 S Ashland Ave
Chicago, IL 60608

2600 S Michigan Ave

1.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2600 S Michigan Ave
Chicago, IL 60616

811 S State St Ste B

1.89 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
811 S State St Ste B
Chicago, IL 60605

2929 S Ellis Ave

1.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2929 S Ellis Ave
Chicago, IL 60616

600 W Adams St

2.06 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
600 W Adams St
Chicago, IL 60661

75 Remittance Dr Ste 1660

2.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
75 Remittance Dr Ste 1660
Chicago, IL 60675

Remit Only: Dept 77-6478

2.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Remit Only: Dept 77-6478
Chicago, IL 60678

614 W Monroe St

2.14 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
614 W Monroe St
Chicago, IL 60661

300 W Adams St Ste 835

2.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
300 W Adams St Ste 835
Chicago, IL 60606

1230 W Lake St

2.49 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM • Sat: 7:30 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
1230 W Lake St
Chicago, IL 60607

36 W Randolph St Ste 705

2.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
36 W Randolph St Ste 705
Chicago, IL 60601

55 E Washington St Ste 2500

2.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
55 E Washington St Ste 2500
Chicago, IL 60602

311 N Aberdeen St Ste 100b

2.61 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
311 N Aberdeen St Ste 100b
Chicago, IL 60607

111 N Wabash Ave Ste 1514,

2.63 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
111 N Wabash Ave Ste 1514,
Chicago, IL 60602

300 E Randolph St Cl404

2.83 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
300 E Randolph St Cl404
Chicago, IL 60601

835 S Wolcott Ave Ste E144

2.85 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
835 S Wolcott Ave Ste E144
Chicago, IL 60612

676 N Saint Clair St Ste 900

3.10 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
676 N Saint Clair St Ste 900
Chicago, IL 60611

1030 W Chicago Ave

3.22 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-7:00 PM • Sat: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
1030 W Chicago Ave
Chicago, IL 60642

219 W Chicago Ave

3.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
219 W Chicago Ave
Chicago, IL 60654

650 W 51st St

3.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
650 W 51st St
Chicago, IL 60609

233 E Erie St Ste 602

3.38 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
233 E Erie St Ste 602
Chicago, IL 60611

1460 N Halsted St Ste 201,

4.00 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:30 PM • Sat: 8:00 AM-1:00 PM✓ Walk-in with Electronic Donor Pass
1460 N Halsted St Ste 201,
Chicago, IL 60642

5709 S. Loomis Blvd

4.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5709 S. Loomis Blvd
Chicago, IL 60636

1044 N Francisco Ave

4.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1044 N Francisco Ave
Chicago, IL 60622

1520 N Damen Ave

4.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1520 N Damen Ave
Chicago, IL 60622

1431 N Western Ave Ste 137,

4.39 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1431 N Western Ave Ste 137,
Chicago, IL 60622

5841 S Maryland Ave

4.83 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5841 S Maryland Ave
Chicago, IL 60637

2400 N. Ashland Ave Suite 100

5.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2400 N. Ashland Ave Suite 100
Chicago, IL 60614

Employment, DOT, Court Ordered, Probation, Family

Accredited Drug Testing presents a full range of drug and alcohol assessments at 33 convenient testing facilities in Chicago, Illinois. We cater to DOT and non-DOT requirements with tests like urine drug screenings, breath and EtG alcohol tests, and hair follicle examinations, serving personal, corporate, and legal purposes. In Chicago, IL, we deliver rapid outcomes and SAMSA-approved lab evaluations with options for same-day service. Most of our Chicago locations are easily accessible, ensuring quick access from your home or workplace. We also provide services like Occupational Health Testing, Clinical Assessments, and Background Verification.

Dial (800) 221-4291 or sign up online. Just pick your test and choose a local facility—screening is offered for yourself, staff, or others. Setting up a test is swift and straightforward; contact our scheduling team or arrange your test online anytime. Our efficient and easy-to-navigate system lets you manage drug testing near Chicago with ease.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Chicago, IL

1

Order Online or Call

Select your test & search nearby Chicago collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Chicago, IL Drug Testing

Drug Testing Services Available at Our Chicago, IL Labs:

At our Chicago drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Illinois Drug Testing Laws & Compliance in Chicago

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Chicago, IL adhering strictly to 820 ILCS 55/1 et seq., federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
820 ILCS 55/1 et seq.
Medical Marijuana
Legal
Recreational Cannabis
Legal
Workplace Rights
Zero-Tolerance Protected

Illinois Cannabis Regulation and Tax Act upholds employer authority to enforce reasonable drug-free workplace policies, zero-tolerance rules, and safety testing.

Under 820 ILCS 55/1 et seq., employers in Chicago may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal; Recreational: Legal in Illinois), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Chicago, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Chicago and Illinois.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Chicago, IL

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Chicago to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Chicago Neighborhoods Covered:

● The Loop ● River North ● West Loop ● Lincoln Park ● Wicker Park ● Lakeview ● Logan Square ● Hyde Park ● Fulton Market ● Streeterville ● Bridgeport ● Bronzeville

Major Employment & Commercial Hubs Served:

The Loop Central Business District • Fulton Market Tech & Corporate Corridor • O'Hare Air Cargo Logistics Hub • Illinois Medical District

Primary Chicago ZIP Codes Served:

60601 60602 60603 60607 60611 60614 60654 60661
Located anywhere in Chicago? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Chicago

North Chicago • Grant Park • Cornell • Burnham • Washington Park • Cicero • Oak Park • Berwyn

Chicago, IL Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Chicago.

Test Type & Local Panel Detection Window Common Use Cases in Chicago Turnaround Time
5-Panel Urine Screen in Chicago →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Chicago →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Chicago →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Chicago →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Chicago →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Chicago →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Chicago, IL

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Chicago.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Chicago facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Chicago, IL

Need immediate walk-in service in Chicago? View our Chicago, IL Drug Testing Facility Guide or order an instant electronic donor pass online.

Chicago Drug Testing

Quickly find trusted local drug testing centers in Chicago, IL — fast, convenient, and reliable every time!

Chicago DOT Drug Testing

Quickly find a local DOT drug testing center in Chicago, IL — fast, reliable, convenient nationwide service!

Chicago DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Chicago, IL.

Chicago Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Chicago, IL.v

Chicago Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Chicago, IL locations—results you can trust, every time!

Chicago Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Chicago, IL.

Chicago Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Chicago, IL.

Chicago 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Chicago, IL employers and individuals nationwide.

Chicago 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Chicago, IL workplace and personal testing needs.

Chicago On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Chicago, IL workplace compliant.

Chicago DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Chicago, IL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Illinois, workplace drug testing is governed by statutory guidelines under 820 ILCS 55/1 et seq.. Private employers generally operate under a Right to Privacy in the Workplace Act & Cannabis Regulation Act, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Illinois who implement a certified Drug-Free Workplace Program in compliance with 820 ILCS 55/1 et seq. can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Illinois state law compliance standards, state statutes protects registered patients while preserving workplace safety testing. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 33 certified collection sites in the Chicago area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Chicago, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

Very professional and very quick

Very professional and very quick service to schedule appointments! I am pleased with the services and would definitely recommend using them for any drug testing needs!

Carrie Feerick - 4/19/2025

they have made my job much easier

I always have a good experience setting up company driver drug screens through ADT. I'm really happy I found them while searching online, they have made my job much easier.

Exodus Heath - 2/13/2025

Explained everything so no questions

Torin was great. Explained everything so no questions

Tony Lombardo - 4/18/2025


(800) 221-4291