✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Boone, KY

With 31 testing centers in the Boone area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

31 Drug-Testing Centers in Boone

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

120 Jill Dr

3.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
120 Jill Dr
Berea, KY 40403

305 Estill St

4.38 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
305 Estill St
Berea, KY 40403

145 Newcomb Ave

11.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
145 Newcomb Ave
Mt. Vernon, KY 40456

103 Alycia Dr Ste 2

14.77 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
103 Alycia Dr Ste 2
Richmond, KY 40475

1036 Center Dr, Suite B

14.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1036 Center Dr, Suite B
Richmond, KY 40475

801 Eastern Byp

15.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
801 Eastern Byp
Richmond, KY 40475

110 Pleasant Retreat Dr

16.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
110 Pleasant Retreat Dr
Lancaster, KY 40444

646 University Shopping Ctr

16.86 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
646 University Shopping Ctr
Richmond, KY 40475

110 Metker Trl

18.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
110 Metker Trl
Stanford, KY 40484

60 Mercy Ct

22.64 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
60 Mercy Ct
Irvine, KY 40336

417 River Dr

22.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
417 River Dr
Irvine, KY 40336

101 Daniel Dr

25.47 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
101 Daniel Dr
Danville, KY 40422

100 Walton Ave

25.87 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
100 Walton Ave
Danville, KY 40422

2645 N Laurel Rd

25.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2645 N Laurel Rd
London, KY 40741

211 S 3rd St

26.44 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
211 S 3rd St
Danville, KY 40422

380 Whirlaway Dr

28.15 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
380 Whirlaway Dr
Danville, KY 40422

1752 Highway 192 Suite 1

28.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1752 Highway 192 Suite 1
London, KY 40741

117 Quinn Dr

28.95 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
117 Quinn Dr
Nicholasville, KY 40356

3925 Frankfort Ford Rd

29.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3925 Frankfort Ford Rd
Wilmore, KY 40390

1024 N Main St

29.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1024 N Main St
Nicholasville, KY 40356

140 E 5th St

29.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
140 E 5th St
London, KY 40741

1250 Keene Rd Ste 101

29.93 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1250 Keene Rd Ste 101
Nicholasville, KY 40356

360 Spring St Ste 1

29.96 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
360 Spring St Ste 1
London, KY 40741

803 Meyers Baker Rd Ste 120

30.24 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
803 Meyers Baker Rd Ste 120
London, KY 40741

204 Bellaire Dr

30.36 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
204 Bellaire Dr
Nicholasville, KY 40356

85 S Laurel Rd

31.47 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-7:00 PM • Sat: 10:00 AM-2:00 PM✓ Walk-in with Electronic Donor Pass
85 S Laurel Rd
London, KY 40744

1099 Duval St Ste 150

32.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1099 Duval St Ste 150
Lexington, KY 40515

173 E Brannon Rd

32.60 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
173 E Brannon Rd
Nicholasville, KY 40356

3439 Buckhorn Dr

32.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3439 Buckhorn Dr
Lexington, KY 40515

3560 S Highway 27 Ste 2

32.73 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 02:00 PM - 04:00 PM
3560 S Highway 27 Ste 2
Somerset, KY 42501

4071 Tates Creek Centre Drive Ste 202

32.87 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4071 Tates Creek Centre Drive Ste 202
Lexington, KY 40517

Employment, DOT, Court Ordered, Probation, Family

Accredited Drug Testing provides a wide range of drug and alcohol screening options at our 31 testing centers located in Boone, Kentucky. Catering to both DOT and non-DOT requirements, we conduct urine drug tests, breathalyzer exams, EtG alcohol screenings, and hair drug analysis for individuals, businesses, and legal procedures. In Boone, KY, we prioritize quick results with SAMSA accredited lab analysis, offering same-day solutions. Most testing sites are conveniently reachable from your home or office. We also offer Occupational Health Testing, Clinical Testing, and Background Checks.

To schedule a test, call (800) 221-4291 or register online. Choose your exam and the closest center. Tests are available for personal, employee, or third-party needs. Scheduling is straightforward and efficient; reach out to our department or book online anytime. Our process is designed for convenience, allowing you to set up drug testing near Boone with ease.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Boone, KY

1

Order Online or Call

Select your test & search nearby Boone collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Boone, KY Drug Testing

Drug Testing Services Available at Our Boone, KY Labs:

At our Boone drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Kentucky Drug Testing Laws & Compliance in Boone

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Boone, KY adhering strictly to KRS § 336.010 et seq., federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
KRS § 336.010 et seq.
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Prohibited
Workplace Rights
Zero-Tolerance Protected

Kentucky law permits employers to maintain zero-tolerance drug policies, offering voluntary drug-free workplace program certification and worker compensation safety benefits.

Under KRS § 336.010 et seq., employers in Boone may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Prohibited in Kentucky), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Boone, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Boone and Kentucky.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Boone, KY

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Boone to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Boone Neighborhoods Covered:

● Downtown Boone ● Central Business District ● North Boone Commercial Corridor ● South Boone Industrial Park ● East Boone Metro Area ● West Boone Trade Center ● Medical & Healthcare District ● Airport / Logistics Corridor

Major Employment & Commercial Hubs Served:

Boone Main Street Commercial District • Boone Regional Trade Center • County Industrial Parkway • Metro Healthcare & Occupational Medical Corridor

Located anywhere in Boone? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Boone

Conway • Berea • Morrill • Farristown • Threelinks • Paint Lick • Brodhead • Johnetta

Boone, KY Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Boone.

Test Type & Local Panel Detection Window Common Use Cases in Boone Turnaround Time
5-Panel Urine Screen in Boone →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Boone →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Boone →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Boone →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Boone →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Boone →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Boone, KY

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Boone.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Boone facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Boone, KY

Need immediate walk-in service in Boone? View our Walk-In Drug Testing Near Me in Boone, KY facility guide or order an instant electronic donor pass online.

Boone Drug Testing

Quickly find trusted local drug testing centers in Boone, KY — fast, convenient, and reliable every time!

Boone DOT Drug Testing

Quickly find a local DOT drug testing center in Boone, KY — fast, reliable, convenient nationwide service!

Boone DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Boone, KY.

Boone Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Boone, KY.v

Boone Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Boone, KY locations—results you can trust, every time!

Boone Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Boone, KY.

Boone Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Boone, KY.

Boone 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Boone, KY employers and individuals nationwide.

Boone 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Boone, KY workplace and personal testing needs.

Boone On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Boone, KY workplace compliant.

Boone DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Boone, KY drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Kentucky, workplace drug testing is governed by statutory guidelines under KRS § 336.010 et seq.. Private employers generally operate under a Kentucky voluntary drug-free workplace standards, permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Kentucky who implement a certified Drug-Free Workplace Program in compliance with KRS § 336.010 et seq. can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Kentucky state law compliance standards, state statutes allows employers to enforce strict zero-tolerance policies. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 31 certified collection sites in the Boone area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Boone, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

ADT is truly the best

Where do I start?!! ADT is truly the best!! I had the pleasure of speaking with Tori today! She was excellent, professional and truly went above and beyond in her kind, compassionate care and commitment to providing me with the best service possible. I was in such a time crunch, but I was able to call, order the test, pay for it over the phone and get the test I needed within the hour. Not only did this put my mind at ease, they saved my job!! I am forever grateful for this team of hard workers, that care, with such great work ethics! Thank you from the very bottom of my heart!!! If you need a drug, alcohol, etc… screening, this team will be your lifesavers!!

Sarah Patterson - 4/8/2025

test was quick and easy

Company New Hire Employee Process for DOT Drug test was quick and easy. No need to make appt. All employee had to do is show prepaid qr code I sent to employee show ID and was set. Would Recommend.

Maria Lopez - 11/19/2024

Very helpful

They were very helpful in finding a place to get tested on the route back home! They made a difficult time seem easy with no judgement!

Anne Creden - 11/19/2024

Smooth and Seamless

I use their service for new hire and DOT employee's. Spoke with Taisha Walker this morning, and she was very helpful. She made the process smooth and seamless.

Christina Galdos - 3/9/2025

I love the customer service

I love the customer service. The lady was patient enough to help me book my appointment with ease and ensuring I get the location that's best for me. Keep up the good work!

Elizabeth Chiaka - 9/19/2024

Thank You!!

I needed a quick drug test for visitation with my children. I called at 6am they answered and set me up with a place a couple miles from my house to go get the test done, that was on Wednesday, I had the results on Friday afternoon and was able to see my kids on Saturday. Thank you for the help!

Mark Broadhead - 2/12/2025

Really Helpful

I am just really happy to see that the location near me still offers hair follicle tests!! They are really helpful when any false positives happen, waiting on my results. Hope they come fast!

Kali Collins - 1/16/2025

Had very fast and good service

Had very fast and good service, was able to get an appointment same day with the nearest facility. My questions were answered thoroughly but quick. Definitely recommend

Jacob Matzke - 11/19/2024

Fast service and friendly staff!

Fast service and friendly staff! Could not recommend enough for drug testing services.

Habib Malik - 4/12/2025

Fast,easy and efficient

Fast,easy and efficient. They made the whole process stress free and simple. Thanks 😊

Micheal Ankenbrandt - 2/19/2025


(800) 221-4291