✓ Same-Day Walk-Ins Welcome ✓ Digital Donor Pass in Minutes ✓ No Doctor Order Required ✓ SAMHSA-Certified Labs

Drug Testing Locations in Twilight, PA

With 37 testing centers in the Twilight area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

Urine (5, 10, 12, 14-Panel) Hair Follicle (90-Day) DOT Drug & Alcohol Court & Probation Oral Fluid / Saliva EtG Alcohol
🕑 Collection Site Hours: Monday – Friday: 8:00 AM – 4:00 PM • ✓ Walk-in with Electronic Donor Pass • Saturday – Sunday: Closed

37 Drug-Testing Centers in Twilight

Same-Day Electronic Donor Pass Accepted at All Locations Below

Minutes Away, Test Today

625 Lincoln Ave Ste 203

1.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
625 Lincoln Ave Ste 203
North Charleroi, PA 15022

1200 Mckean Ave Ste 107

2.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1200 Mckean Ave Ste 107
Charleroi, PA 15022

760 Tri County Lane, Suite 104,

3.03 mi
🕑 Testing Hours: Mon–Fri: 9:30 AM-1:30 PM • Sat: 9:30 AM-11:30 AM✓ Walk-in with Electronic Donor Pass
760 Tri County Lane, Suite 104,
Belle Vernon, PA 15012

860 Rostraver Rd

3.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
860 Rostraver Rd
Belle Vernon, PA 15012

1163 Country Club Rd

5.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1163 Country Club Rd
Monongahela, PA 15063

1000 Mckean Ave

5.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1000 Mckean Ave
Donora, PA 15033

800 Plaza Dr Ste 210

6.45 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
800 Plaza Dr Ste 210
Belle Vernon, PA 15012

447 W Main Street,

6.61 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
447 W Main Street,
Monongahela, PA 15063

4918 State Route 51 S

6.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4918 State Route 51 S
Belle Vernon, PA 15012

220 Greene Plz

7.50 mi
🕑 Facility: Mon–Fri: 07:30 AM - 03:30 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 09:00 AM - 11:00 AM, 01:00 PM - 02:30 PM
220 Greene Plz
Waynesburg, PA 15370

220 Greene Plz Ste 103

7.50 mi
🕑 Facility: Mon–Fri: 07:00 AM - 02:30 PM⚡ Drug Screens: Mon–Fri: 08:00 AM - 01:30 PM
220 Greene Plz Ste 103
Waynesburg, PA 15370

129 Simpson Rd, Ste 105

7.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
129 Simpson Rd, Ste 105
Brownsville, PA 15417

125 Simpson Rd

8.23 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:30 PM⚡ Drug Screens: Mon–Fri: 07:30 AM - 11:30 AM, 01:30 PM - 03:00 PM
125 Simpson Rd
Brownsville, PA 15417

146 Motor Drome Rd

9.32 mi
🕑 Facility: Mon–Fri: 06:30 AM - 02:30 PM⚡ Drug Screens: Mon–Fri: 06:30 AM - 12:00 PM, 12:30 PM - 02:30 PM
146 Motor Drome Rd
Smithton, PA 15479

565 Coal Valley Rd South Hills Medical Building Suite 502

12.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
565 Coal Valley Rd South Hills Medical Building Suite 502
Clairton, PA 15025

1200 Brooks Ln Ste 100

14.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1200 Brooks Ln Ste 100
Clairton, PA 15025

1895 Jefferson Rd

14.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1895 Jefferson Rd
Rices Landing, PA 15357

575 Coal Valley Road Suite 502

14.50 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
575 Coal Valley Road Suite 502
Pittsburgh, PA 15236

695 Clairton Blvd

14.68 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-7:00 PM✓ Walk-in with Electronic Donor Pass
695 Clairton Blvd
Pleasant Hills, PA 15236

275 Curry Hollow Rd, Suite 160

16.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
275 Curry Hollow Rd, Suite 160
Pittsburgh, PA 15236

325 Mcclellandtown Rd

16.05 mi
🕑 Facility: Mon–Fri: 06:30 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 07:30 AM - 02:00 PM
325 Mcclellandtown Rd
Uniontown, PA 15401

2001 Lincoln Way, Suite 16, Oak Park Mall

16.08 mi
🕑 Testing Hours: Mon–Fri: 10:00 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
2001 Lincoln Way, Suite 16, Oak Park Mall
White Oak, PA 15131

1829 University Rd

16.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1829 University Rd
Dunbar, PA 15431

659 Cherry Tree Ln

16.27 mi
🕑 Facility: Mon–Fri: 08:00 AM - 04:30 PM (Lunch: 12:00 PM - 12:30 PM)⚡ Drug Screens: Mon–Fri: 08:00 AM - 11:00 AM, 12:30 PM - 03:30 PM
659 Cherry Tree Ln
Uniontown, PA 15401

811 S Center Ave

16.32 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM • Sat: 8:00 AM-1:00 PM • Sun: 9:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
811 S Center Ave
New Stanton, PA 15674

1829 University Dr

16.33 mi
🕑 Facility: Mon–Fri: 07:00 AM - 04:30 PM (Lunch: 12:00 PM - 01:00 PM)⚡ Drug Screens: Mon–Fri: 07:30 AM - 11:00 AM, 01:30 PM - 03:30 PM
1829 University Dr
Dunbar, PA 15431

675a Cherry Tree Ln

16.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
675a Cherry Tree Ln
Uniontown, PA 15401

404 West Main Street,

16.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
404 West Main Street,
Uniontown, PA 15401

161 Waterdam Rd Apt 160

16.43 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
161 Waterdam Rd Apt 160
Mcmurray, PA 15317

211 Easy St Ste 224

16.52 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
211 Easy St Ste 224
Uniontown, PA 15401

500 W Berkeley St

16.59 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:00 PM (Lunch: 12:00 PM - 12:30 PM)⚡ Drug Screens: Mon–Fri: 07:00 AM - 11:00 AM, 12:30 PM - 02:00 PM
500 W Berkeley St
Uniontown, PA 15401

1907 Lebanon Church Road, Suite 104

16.60 mi
🕑 Testing Hours: Mon–Fri: 9:30 AM-3:30 PM✓ Walk-in with Electronic Donor Pass
1907 Lebanon Church Road, Suite 104
West Mifflin, PA 15122

1015 Waterdam Plaza Drive,

16.63 mi
🕑 Testing Hours: Mon–Fri: 9:30 AM-2:30 PM✓ Walk-in with Electronic Donor Pass
1015 Waterdam Plaza Drive,
Mc Murray, PA 15317

11347 Route 30 Ste 3

16.65 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
11347 Route 30 Ste 3
Irwin, PA 15642

12116 State Route 30

16.65 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-8:00 PM • Sat: 8:00 AM-7:00 PM • Sun: 8:00 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
12116 State Route 30
North Huntingdon, PA 15642

4000 Washington Rd Ste 203

16.66 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
4000 Washington Rd Ste 203
Mcmurray, PA 15317

751 Pittsburgh Mckeesport Blvd

16.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
751 Pittsburgh Mckeesport Blvd
Dravosburg, PA 15034

Employment, DOT, Court Ordered, Probation, Family

At our 37 locations in the Twilight, Pennsylvania region, Accredited Drug Testing provides a full range of drug and alcohol testing services. We conduct both DOT and non-DOT urine drug tests, breath alcohol tests, EtG alcohol screenings, and hair drug testing for personal, professional, and legal purposes. In Twilight, PA, we offer quick-result tests and SAMSA endorsed laboratory analysis, with same-day service options; most centers are conveniently located close to homes or workplaces. We're also equipped to handle Occupational Health Testing, Clinical Testing, and Background Verification.

Dial (800) 221-4291 or register on our website. Choose your test type and a convenient location—services cater to individuals, employees, or others. Arranging a test is swift and straightforward; contact our scheduling team or book online at any time. Our efficient process ensures effortless drug testing arrangements near Twilight.

* You must register by phone or online to receive your donor pass/registration prior to proceeding to the testing center. You must bring a valid government issued ID along with the registration/barcode number which was sent to you by email.

When you're searching for drug testing near me or drug testing locations, we provide a simple and convenient process to find a drug and alcohol testing location near you that is certified to provide all of your drug and alcohol testing needs.

How the Drug Testing Process Works in Twilight, PA

1

Order Online or Call

Select your test & search nearby Twilight collection sites.

2

Get Donor Pass

Receive your donor pass by email or SMS immediately.

3

Visit Collection Center

Walk in with donor pass & photo ID. No appointment needed.

4

Fast Results

Secure results delivered confidentially in 24-48 hours.

Twilight, PA Drug Testing

Drug Testing Services Available at Our Twilight, PA Labs:

At our Twilight drug testing collection sites, Accredited Drug Testing provides one of the widest selections of drug and alcohol testing services available. Whether you're an employer, attorney, court, or private individual, we offer both DOT and non-DOT testing options—ranging from rapid tests to comprehensive lab-based screenings—capable of detecting nearly any substance.

Mobile/On-Site Drug Testing

If you're an employer needing to test 25 or more employees and looking to save time and money, we offer mobile on-site drug testing where we come to you. Call us today for more information.

Official Legal & Regulatory Framework

Pennsylvania Drug Testing Laws & Compliance in Twilight

SAMHSA Certified • MRO Verified

Accredited Drug Testing provides employer and individual screening in Twilight, PA adhering strictly to 43 P.S. § 951 et seq., federal DOT 49 CFR Part 40 guidelines, and certified SAMHSA laboratory chain-of-custody standards.

Statutory Citation
43 P.S. § 951 et seq.
Medical Marijuana
Legal (Medical)
Recreational Cannabis
Prohibited
Workplace Rights
Zero-Tolerance Protected

Pennsylvania law supports employer zero-tolerance workplace drug policies, allowing pre-employment, safety-sensitive, and reasonable suspicion testing programs.

Under 43 P.S. § 951 et seq., employers in Twilight may institute comprehensive drug-free workplace programs including:

  • Pre-Employment Screening: Conditional job offer testing for all applicants.
  • Reasonable Suspicion Testing: Objective, documented behavioral signs of impairment.
  • Post-Accident Testing: Mandatory screening following workplace injuries or property damage.
  • Random Testing: Unannounced selection for safety-sensitive roles and machinery operators.
  • Workers' Comp Incentives: Verified testing programs provide premium discounts on state workers' compensation insurance policies.

While cannabis statutes continue evolving across the United States (Medical: Legal (Medical); Recreational: Prohibited in Pennsylvania), employers retain the legal prerogative to prohibit substance impairment on job premises, during operational shifts, and while operating company vehicles.

Federal regulations, Department of Transportation (DOT) mandates, and safety-sensitive positions strictly prohibit marijuana use regardless of state cannabis status. ADT provides both standard THC screening and non-THC customized drug panels for private employers wishing to adapt to state-specific hiring preferences.

For commercial drivers, maritime operators, aviation crews, and transit personnel operating in Twilight, all testing follows rigorous Department of Transportation (DOT) standards governed by 49 CFR Part 40:

  • Federal 5-Panel Regulated Urine Screen: Tested for Marijuana, Cocaine, Amphetamines/Methamphetamines, Opioids (Codeine, Morphine, Heroin, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone), and PCP.
  • SAMHSA Accredited Laboratories: Zero compromise on testing accuracy with gas chromatography/mass spectrometry (GC/MS) confirmation.
  • Medical Review Officer (MRO): Every non-negative specimen is reviewed by a licensed physician MRO before reporting.
  • FMCSA Clearinghouse Reporting: Real-time reporting of verified positive tests and refusals to test into the federal driver clearinghouse database.

Accredited Drug Testing provides legally admissible drug and alcohol screening for county court dockets, child custody hearings, probation requirements, and pretrial diversion programs throughout Twilight and Pennsylvania.

All specimens undergo observed or witnessed collection procedures when ordered by the court, accompanied by rigorous Federal Chain of Custody Forms (CCF). Turnaround time is rapid, and official certificates of analysis can be forwarded directly to your attorney, court case officer, or probation supervisor upon release.

Hyper-Local Proximity Coverage

Neighborhoods & Service Districts in Twilight, PA

Minutes Away, Same-Day Donor Pass

Accredited Drug Testing operates collection centers strategically positioned throughout Twilight to ensure fast donor access with minimal drive times. Walk-ins are welcomed with an immediate electronic donor pass across all major neighborhoods and transit corridors:

Key Twilight Neighborhoods Covered:

● Downtown Twilight ● Central Business District ● North Twilight Commercial Corridor ● South Twilight Industrial Park ● East Twilight Metro Area ● West Twilight Trade Center ● Medical & Healthcare District ● Airport / Logistics Corridor

Major Employment & Commercial Hubs Served:

Twilight Main Street Commercial District • Twilight Regional Trade Center • County Industrial Parkway • Metro Healthcare & Occupational Medical Corridor

Located anywhere in Twilight? Receive your electronic pass in under 5 minutes.
Order Test Online →

Surrounding Areas Served Near Twilight

Speers • Belle Vernon • North Charleroi • Charleroi • Dunlevy • North Belle Vernon • Naomi • Allenport

Twilight, PA Drug & Alcohol Test Panels & Detection Windows

Select a test panel below to review specific drug compounds screened, detection timeframes, and instant electronic pass registration in Twilight.

Test Type & Local Panel Detection Window Common Use Cases in Twilight Turnaround Time
5-Panel Urine Screen in Twilight →
THC, Cocaine, Basic Opiates, Amphetamines, PCP
1 to 5 Days Pre-Employment, Workplace Safety & Non-DOT Randoms 24 to 48 Hours
10-Panel Urine Screen in Twilight →
Adds Benzodiazepines, Barbiturates, Methadone, Propoxyphene
1 to 7 Days Healthcare Staffing, Corporate Policies & Court Orders 24 to 48 Hours
Hair Follicle Drug Test in Twilight →
Long-term historical pattern analysis (90-day window)
Up to 90 Days Child Custody, Legal Defense & Executive Screening 3 to 5 Business Days
DOT Drug & Alcohol Test in Twilight →
49 CFR Part 40 compliant with CCF & MRO verification
1 to 5 Days FMCSA, FAA, FRA, FTA, USCG Regulated Drivers & Crew 24 to 48 Hours
ETG & Breath Alcohol in Twilight →
Evidential breath testing or 80-hour urine EtG metabolite
Breath: 0–12 Hrs
EtG: Up to 80 Hrs
Zero-Tolerance Workplace, Post-Accident & Probation Immediate to 48 Hours
DOT Physical Exam in Twilight →
FMCSA NRCME certified medical examiner card (Form MCSA-5876)
1 to 2 Year Card Commercial Driver License (CDL) Medical Certification Same-Day Medical Card
Full-Service Testing Solutions

Occupational Health & Specialized Screening in Twilight, PA

Beyond standard urine screening, Accredited Drug Testing delivers end-to-end occupational medical solutions, DOT credentialing, court-admissible forensic testing, and employer background compliance.

NRCME Certified

DOT Physicals & Medical Cards

FMCSA-compliant examinations conducted by certified medical examiners. Instant driver medical certificate renewal across Twilight.

AABB Accredited

Court Legal DNA & Paternity

100% court-admissible legal paternity, maternity, sibling, and grandparentage testing with strict chain of custody for family court.

FCRA Compliant

Background Checks & MVR

Comprehensive pre-employment screening, nationwide criminal history searches, and Motor Vehicle Records (MVR) for driver compliance.

Long Detection Window

90-Day Hair Follicle Drug Test

Tamper-resistant 90-day historical substance screening. Preferred by family courts, custody attorneys, and zero-tolerance employers.

Instant & Lab Confirmed

Breath Alcohol & 80-Hr EtG

Evidential breath alcohol testing (BAT) and 80-hour EtG urine/hair metabolite analysis for probation monitoring and workplace safety.

We Come to You

Mobile On-Site Group Testing

Certified mobile collection technicians dispatched directly to your Twilight facility or job site for groups of 25+ employees.

Local Testing Resource Network

More Drug & Alcohol Testing Options in Twilight, PA

Need immediate walk-in service in Twilight? View our Walk-In Drug Testing Near Me in Twilight, PA facility guide or order an instant electronic donor pass online.

Twilight Drug Testing

Quickly find trusted local drug testing centers in Twilight, PA — fast, convenient, and reliable every time!

Twilight DOT Drug Testing

Quickly find a local DOT drug testing center in Twilight, PA — fast, reliable, convenient nationwide service!

Twilight DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Twilight, PA.

Twilight Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Twilight, PA.v

Twilight Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Twilight, PA locations—results you can trust, every time!

Twilight Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Twilight, PA.

Twilight Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Twilight, PA.

Twilight 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Twilight, PA employers and individuals nationwide.

Twilight 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Twilight, PA workplace and personal testing needs.

Twilight On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Twilight, PA workplace compliant.

Twilight DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Twilight, PA drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

In Pennsylvania, workplace drug testing is governed by statutory guidelines under 43 P.S. § 951 et seq.. Private employers generally operate under a Pennsylvania Medical Marijuana Act (35 P.S. § 10231.2103), permitting pre-employment, random, post-accident, and reasonable suspicion testing.
Yes. Employers in Pennsylvania who implement a certified Drug-Free Workplace Program in compliance with 43 P.S. § 951 et seq. can qualify for insurance premium discounts and enhanced legal protections during workers' compensation claims.
Under Pennsylvania state law compliance standards, state statutes prohibits discrimination against certified medical marijuana cardholders. Safety-sensitive positions, DOT-regulated roles, and federal contract compliance remain strictly zero-tolerance across all jurisdictions.
Accredited Drug Testing provides access to 37 certified collection sites in the Twilight area, offering urine, hair follicle, breath alcohol, and DOT testing.
While walk-ins are welcome with an electronic donor pass at collection sites across Twilight, registering your test online or calling (800) 221-4291 prior to visiting guarantees rapid priority specimen collection with an instant barcode pass.
Negative drug and alcohol test results are typically released within 24 to 48 hours of specimen processing. Any non-negative screenings undergo confirmatory GC/MS or LC/MS testing and licensed Medical Review Officer (MRO) verification within 3 to 5 business days.

Customer Reviews

Sweet and helpful

Trish was amazing and got me through the sytem very fast and swift. I had a hard time hearing her a couple of times, but she was super sweet and helpful throughout the process. Highly recommend her!

Sophia Schutze - 6/19/2024

Saved My CDL!

Time was running out before my Cdl got downgraded because of a violation I had on clearinghouse. I couldn't find an employer to send me for my return to duty test, but these guys had my test scheduled and done in the same day! They saved my cdl. Thank you again!

Michael Williams - 12/2/2024

Thank You!!

I needed a quick drug test for visitation with my children. I called at 6am they answered and set me up with a place a couple miles from my house to go get the test done, that was on Wednesday, I had the results on Friday afternoon and was able to see my kids on Saturday. Thank you for the help!

Mark Broadhead - 2/12/2025

Super Helpful

Detricia was super helpful in making sure that the drug test is sent my worker for was the right one I needed for my company to be compliant with my customers drug test requirements.

Kevin Howell - 12/19/2024

A very nice company

A very nice company of expertise. The staff will go above to help you out of your situation in a timely matter 👏

Tam Jack - 2/19/2025

Clear and Competent

I was so surprised with the quality of customer service I received today when I called Accredited Drug Testing. The representative was so clear and competent. She was able to reschedule an appointment at my preferred location. The representative whose name is Justice, was so polite and competent. She expedited the whole process. It was a relief to me.

Rachel Padian - 9/6/2024

Smooth and Seamless

I use their service for new hire and DOT employee's. Spoke with Taisha Walker this morning, and she was very helpful. She made the process smooth and seamless.

Christina Galdos - 3/9/2025

Friendly and Knowledgeable

I reached out Accredited Drug Testing/Secure Screenings to partner for my drug testing business (Discovery Diagnostics Columbia SC) and the process was extremely easy. I worked with Sicilia who was amazing. She was friendly & knowledgeable, and answer all of my questions with ease.

Kendra Harley - 3/26/2025

Had very fast and good service

Had very fast and good service, was able to get an appointment same day with the nearest facility. My questions were answered thoroughly but quick. Definitely recommend

Jacob Matzke - 11/19/2024

phone support has been excellent

I have used ADT several times. Their phone support has been excellent, both when putting in the orders and when asking for help to interpret results.

Tee Ess - 12/19/2024


(800) 221-4291