DOT Physical Exams - Orlando, FL

Locations, All Cities, All States

FMCSA | FAA | USCG | FTA | FRA | PHMSA

With 29 testing centers in the Orlando area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

(800) 221-4291

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29 DOT Drug-Testing Centers in Orlando

Minutes Away, Test Today

601 E Colonial Dr

1.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
601 E Colonial Dr
Orlando, FL 32803
Call (800) 221-4291 Get Donor Pass

2323 S Orange Ave Ste A

1.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2323 S Orange Ave Ste A
Orlando, FL 32806
Call (800) 221-4291 Get Donor Pass

701 E Michigan St

1.88 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
701 E Michigan St
Orlando, FL 32806
Call (800) 221-4291 Get Donor Pass

709 E Michigan St

1.88 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
709 E Michigan St
Orlando, FL 32806
Call (800) 221-4291 Get Donor Pass

813 E Michigan St

1.89 mi
🕑 Testing Hours: Mon–Fri: 8:00 AM-4:00 PM • Sat: 8:00 AM-12:00 PM✓ Walk-in with Electronic Donor Pass
813 E Michigan St
Orlando, FL 32806
Call (800) 221-4291 Get Donor Pass

630 N Bumby Ave

1.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
630 N Bumby Ave
Orlando, FL 32803
Call (800) 221-4291 Get Donor Pass

3615 S Orange Ave

2.48 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3615 S Orange Ave
Orlando, FL 32806
Call (800) 221-4291 Get Donor Pass

Bldg 49635 - Rm 1219

2.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
Bldg 49635 - Rm 1219
Orlando, FL 32899
Call (800) 221-4291 Get Donor Pass

2314 N Orange Blossom Trl

2.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2314 N Orange Blossom Trl
Orlando, FL 32804
Call (800) 221-4291 Get Donor Pass

4401 S Orange Ave Ste 110

2.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4401 S Orange Ave Ste 110
Orlando, FL 32806
Call (800) 221-4291 Get Donor Pass

324 E Par St.., Ste 100,

3.04 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
324 E Par St.., Ste 100,
Orlando, FL 32804
Call (800) 221-4291 Get Donor Pass

509 S Semoran Blvd

4.17 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
509 S Semoran Blvd
Orlando, FL 32807
Call (800) 221-4291 Get Donor Pass

36 S Semoran Blvd Ste B

4.19 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
36 S Semoran Blvd Ste B
Orlando, FL 32807
Call (800) 221-4291 Get Donor Pass

483 N Semoran Blvd Suite 103

4.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
483 N Semoran Blvd Suite 103
Winter Park, FL 32792
Call (800) 221-4291 Get Donor Pass

1338 Pleasant Oak Ln

4.49 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1338 Pleasant Oak Ln
Orlando, FL 32804
Call (800) 221-4291 Get Donor Pass

611 N Wymore Rd Ste 220

4.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
611 N Wymore Rd Ste 220
Winter Park, FL 32789
Call (800) 221-4291 Get Donor Pass

501 N Orlando Ave Ste 227

4.56 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
501 N Orlando Ave Ste 227
Winter Park, FL 32789
Call (800) 221-4291 Get Donor Pass

2540 Lee Rd

4.66 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2540 Lee Rd
Winter Park, FL 32789
Call (800) 221-4291 Get Donor Pass

1850 Lee Rd Ste 216

4.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1850 Lee Rd Ste 216
Winter Park, FL 32789
Call (800) 221-4291 Get Donor Pass

1850 Lee Rd Ste 300

4.67 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1850 Lee Rd Ste 300
Winter Park, FL 32789
Call (800) 221-4291 Get Donor Pass

1462 W Oak Ridge Rd

4.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1462 W Oak Ridge Rd
Orlando, FL 32809
Call (800) 221-4291 Get Donor Pass

5021 Eggleston Ave Ste A

4.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5021 Eggleston Ave Ste A
Orlando, FL 32804
Call (800) 221-4291 Get Donor Pass

4780 N Orange Blossom Trl

4.87 mi
🕑 Testing Hours: Mon–Fri: 7:30 AM-5:00 PM✓ Walk-in with Electronic Donor Pass
4780 N Orange Blossom Trl
Orlando, FL 32810
Call (800) 221-4291 Get Donor Pass

6220 S Orange Blossom Trl Ste 518

5.05 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6220 S Orange Blossom Trl Ste 518
Orlando, FL 32809
Call (800) 221-4291 Get Donor Pass

2555 S Kirkman Rd

5.11 mi
🕑 Testing Hours: M-Sun: 8:00 AM-8:00 PM✓ Walk-in with Electronic Donor Pass
2555 S Kirkman Rd
Orlando, FL 32811
Call (800) 221-4291 Get Donor Pass

1718 Sand Lake Rd C-106

5.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1718 Sand Lake Rd C-106
Orlando, FL 32809
Call (800) 221-4291 Get Donor Pass

1603 S Hiawassee Rd, Suite 120

5.64 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-2:30 PM • Sat: 6:30 AM-12:30 PM✓ Walk-in with Electronic Donor Pass
1603 S Hiawassee Rd, Suite 120
Orlando, FL 32835
Call (800) 221-4291 Get Donor Pass

5425 S Semoran Blvd

5.68 mi
🕑 Facility: Mon–Fri: 06:30 AM - 03:00 PM⚡ Drug Screens: Mon–Fri: 06:30 AM - 03:00 PM
5425 S Semoran Blvd
Orlando, FL 32822
Call (800) 221-4291 Get Donor Pass

4554 S Kirkman Rd

5.69 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4554 S Kirkman Rd
Orlando, FL 32811
Call (800) 221-4291 Get Donor Pass

Orlando, FL DOT Physical - What You Need to Know

A DOT physical is an all-encompassing health assessment mandated for every commercial motor vehicle operator by the Department of Transportation. This evaluation verifies that drivers are in the right physical, mental, and emotional condition to manage large vehicles safely on roads. Conducted by a certified medical examiner from the National Registry of Certified Medical Examiners, a successful result grants a DOT medical certificate needed to keep a CDL valid under FMCSA rules. For assistance with other DOT agency regulations, call us at 800-221-4291.

During your exam, the medical examiner will complete a thorough health assessment to determine if you meet the Federal Motor Carrier Safety Administration (FMCSA) medical standards. The process typically includes:

What Does a DOT Physical Include?

Health History Review
Vision Test

Hearing Test
Urinalysis

Vital Signs
Physical Examination

DOT Testing | DOT Breath Alcohol | DOT Consortium Services | MVR Reports Also Available


What is checked in a DOT Physical?

  • Health review & medication evaluation in Orlando, AR
  • Vision test (correction allowed, minimum standard required)
  • Auditory evaluation (forced whisper or auditory test)
  • Blood pressure and pulse monitoring
  • Physical check-up (system review)
  • Urine test (check specific gravity, protein, sugar)

After success, your Medical Examiner's Certificate might be issued up to 24 months. Certain conditions call for shorter certification or more specialist documentation

Who needs a DOT Physical?

You must have a valid DOT medical certificate (card) if you:

  • Have a commercial driver's license (CDL)- class A,B, or C for interstate commerce
  • Drive a vehicle over 10,001 lbs. GVWR in interstate commerce
  • Transport more than 8 passengers (for hire) or more than 15 passengers (not for hire)
  • Transport hazardous materials that require placarding under DOT regulations
  • Work for employers regulated by DOT agencies such as FMCSA, FAA, FRA, FTA or USCG where DOT medical qualification is required

Orlando, NV specifications should be adhered to.

DOT Physical Near Orlando, FL

Orlovista, FL

Orange Park, FL

Azalea Park, FL

Pine Hills, FL

Pine Castle, FL

Winter Park, FL

Baldwin, FL

Conway, FL

Fairview Shores, FL

Edgewood, FL

Holden Heights, FL

What to bring with you to a DOT Physical?

  • Government-issued photo
  • Glasses/contacts & prescription
  • List of medications & dosages
  • CPAP usage data (if applicable)
  • Specialist clearances (cardiology, sleep apnea, diabetes, etc.)
  • Recent A1C/blood pressure logs if monitored

Preparing these items in advance will facilitate the examination process. Orlando, RI residents and others should ensure they have these documents ready before attending their medical examination.

What happens if I fail a DOT Physical?

  • No Medical card will be issued
  • Temporary disqualification
  • Referral for treatment to your primary care doctor
  • FMCSA exemptions for certain medical conditions
  • Employer Notification
  • Next Steps (address the medical issues and retake the exam)

If you're in Orlando, VT, make sure to address these issues promptly before attempting the exam again.

What Will Fail a DOT Physical?

Medical Conditions That Can Fail a DOT Physical

  • Vision & Hearing: Vision less than 20/40 (with aid), trouble identifying signal colors, or hearing deficiency for a whisper at 5 feet hinder driving.
  • Blood Pressure & Heart Disease: High blood pressure (≥180/110 mmHg), recent cardiovascular events, or implanted devices may disqualify.
  • Diabetes: Unmanaged diabetes with frequent hypo events or complications like neuropathy may affect driving ability.
  • Sleep Apnea & Respiratory Issues: Untreated conditions causing drowsiness or severe lung impairments could disqualify.
  • Neurological Disorders: Seizure disorders or conditions causing unconsciousness affect eligibility unless exempted.
  • Substance Abuse: Current illegal substances, alcohol dependency, or misuse of meds compromise safe driving.
  • Psychiatric & Cognitive Disorders: Severe mental illness or cognitive decline that affects decision-making may result in disqualification.

Temporary vs. Permanent Disqualification: Some medical issues may result in a temporary ban until cleared by a physician. Others, like untreated seizure disorders, usually lead to permanent disqualification as per FMCSA standards.

References:
- FMCSA Medical Examiner Physical Qualification Standards (§391.41)
- 49 CFR Part 391 - Qualification of Drivers
- Medical Examiner's Handbook
- FMCSA Driver Exemptions

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Orlando DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Orlando, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT physical typically includes a review of the driver’s health history and medications, vision screening, hearing assessment, vital signs (blood pressure, pulse), urinalysis (checking specific gravity, protein, glucose), and a full physical examination by a certified medical examiner. Upon passing, a Medical Examiner’s Certificate (DOT medical card) may be issued for up to 24 months.
You must have a valid DOT medical certificate if you: hold a commercial driver’s license (CDL) Class A, B or C for interstate commerce; operate a vehicle over 10,001 lbs GVWR in interstate commerce; transport more than eight passengers (for hire) or over fifteen passengers (not for hire); or transport hazardous materials requiring placarding under DOT regulations.
DOT physicals are offered at test centres in all cities and all states, through Accredited Drug Testing’s nationwide network. You can schedule by calling 1-800-221-4291.
You should bring a government-issued photo ID; glasses or contact lenses and the prescription; a list of medications and dosages; CPAP usage data if applicable; and any specialist clearance letters or physician documentation (for conditions like heart disease, sleep apnea, diabetes) to your DOT physical.
If you fail the DOT physical, you will not receive the medical card; you may be temporarily or permanently disqualified depending on the condition; you will need to address any medical issues (e.g., uncontrolled hypertension, untreated sleep apnea, recent myocardial infarction) and then be re-examined.

Customer Reviews

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Where do I start?!! ADT is truly the best!! I had the pleasure of speaking with Tori today! She was excellent, professional and truly went above and beyond in her kind, compassionate care and commitment to providing me with the best service possible. I was in such a time crunch, but I was able to call, order the test, pay for it over the phone and get the test I needed within the hour. Not only did this put my mind at ease, they saved my job!! I am forever grateful for this team of hard workers, that care, with such great work ethics! Thank you from the very bottom of my heart!!! If you need a drug, alcohol, etc… screening, this team will be your lifesavers!!

Sarah Patterson - 4/8/2025

phone support has been excellent

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(800) 221-4291