DOT Physical Exams - Port Charlotte, FL

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With 35 testing centers in the Port Charlotte area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

35 DOT Drug-Testing Centers in Port Charlotte

Minutes Away, Test Today

4161 Tamiami Trl,

0.77 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4161 Tamiami Trl,
Charlotte Harbor, FL 33952

21298 Olean Blvd

0.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
21298 Olean Blvd
Port Charlotte, FL 33952

3280 Tamiami Trl Ste 11

0.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3280 Tamiami Trl Ste 11
Port Charlotte, FL 33952

2525 Harbor Blvd

0.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2525 Harbor Blvd
Port Charlotte, FL 33952

2885 Tamiami Trl

1.35 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2885 Tamiami Trl
Port Charlotte, FL 33952

2811 Tamiami Trl Ste F

1.45 mi
🕑 Facility: Mon–Fri: 07:00 AM - 03:30 PM⚡ Drug Screens: Mon–Fri: 07:00 AM - 03:30 PM
2811 Tamiami Trl Ste F
Port Charlotte, FL 33952

809 E Marion Ave

3.91 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
809 E Marion Ave
Punta Gorda, FL 33950

530 Tamiami Trl

6.72 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
530 Tamiami Trl
Port Charlotte, FL 33953

700 Medical Blvd

14.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
700 Medical Blvd
Englewood, FL 34223

19503 W Villages Parkway

16.14 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
19503 W Villages Parkway
Venice, FL 34293

1500 Placida Rd, Roadmerchants Crossing Shoppin

16.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1500 Placida Rd, Roadmerchants Crossing Shoppin
Englewood, FL 34223

693 Old Englewood Rd Ste C

17.04 mi
🕑 Facility: Mon–Fri: 06:00 AM - 02:30 PM⚡ Drug Screens: Mon–Fri: 06:00 AM - 02:30 PM
693 Old Englewood Rd Ste C
Englewood, FL 34223

30 W Oak St

21.72 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
30 W Oak St
Arcadia, FL 34266

530 Us Highway 41 Byp S

22.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
530 Us Highway 41 Byp S
Venice, FL 34285

936 N Mills Ave

22.64 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
936 N Mills Ave
Arcadia, FL 34266

900 N Roberts Ave

22.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
900 N Roberts Ave
Arcadia, FL 34266

1148 E Gibson St

22.78 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1148 E Gibson St
Arcadia, FL 34266

3412 Sw 3rd Ter

23.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3412 Sw 3rd Ter
Cape Coral, FL 33991

1682 Ne Pine Island Rd

23.20 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-7:00 PM✓ Walk-in with Electronic Donor Pass
1682 Ne Pine Island Rd
Cape Coral, FL 33909

333 Tamiami Trl S Ste 173

23.22 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
333 Tamiami Trl S Ste 173
Venice, FL 34285

428 Del Prado Blvd N Ste 103

23.58 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
428 Del Prado Blvd N Ste 103
Cape Coral, FL 33909

1869 Tamiami Trl N

24.72 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1869 Tamiami Trl N
North Venice, FL 34275

1431 Se 10th St A-1

25.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1431 Se 10th St A-1
Cape Coral, FL 33990

2612 Santa Barbara Blvd #11

26.74 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2612 Santa Barbara Blvd #11
Cape Coral, FL 33914

2665 Cleveland Ave

27.54 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
2665 Cleveland Ave
Fort Myers, FL 33901

1708 Cape Coral Pkwy W Ste 2 # 2

28.99 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1708 Cape Coral Pkwy W Ste 2 # 2
Cape Coral, FL 33914

On-site Only

29.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
On-site Only
Cape Coral, FL 33904

1635 Medical Ln,

29.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1635 Medical Ln,
Fort Myers, FL 33907

4350 Fowler St Ste 2

29.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4350 Fowler St Ste 2
Fort Myers, FL 33901

4771 S Cleveland Ave

29.78 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-7:00 PM✓ Walk-in with Electronic Donor Pass
4771 S Cleveland Ave
Fort Myers, FL 33907

5172 Mason Corbin Ct Ste 2

29.87 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5172 Mason Corbin Ct Ste 2
Fort Myers, FL 33907

13401 Summerlin Rd Ste 9

30.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
13401 Summerlin Rd Ste 9
Fort Myers, FL 33919

8931 Conference Dr Ste 3

31.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
8931 Conference Dr Ste 3
Fort Myers, FL 33919

6202 Presidential Ct Ste A

31.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6202 Presidential Ct Ste A
Fort Myers, FL 33919

7147 Curtiss Ave

31.57 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7147 Curtiss Ave
Sarasota, FL 34231

Port Charlotte, FL DOT Physical - What You Need to Know

A DOT physical is a detailed health assessment mandated by the Department of Transportation for all drivers of commercial motor vehicles. This examination ensures drivers are capable of handling large vehicles on public roads safely. A certified medical examiner, listed on the National Registry, conducts the evaluation. Successfully passing the exam results in gaining a DOT medical certificate, essential for maintaining a valid CDL as per FMCSA rules. For assistance with DOT agency requirements, call 800-221-4291.

During your exam, the medical examiner will complete a thorough health assessment to determine if you meet the Federal Motor Carrier Safety Administration (FMCSA) medical standards. The process typically includes:

What Does a DOT Physical Include?

Health History Review
Vision Test

Hearing Test
Urinalysis

Vital Signs
Physical Examination

DOT Testing | DOT Breath Alcohol | DOT Consortium Services | MVR Reports Also Available


What is checked in a DOT Physical?

  • Review of health history and medication check
  • Vision test (corrective lenses allowed; must meet minimum standards)
  • Hearing check (forced whisper or audiometry)
  • Blood pressure and heart rate assessment
  • Comprehensive physical examination (systems review)
  • Urinalysis (specific gravity, protein, glucose)

In Port Charlotte, AL, successful completion can lead to a Medical Examiner's Certificate valid for up to 24 months. Some conditions might require a shorter certification period or additional specialist documentation.

Who needs a DOT Physical?

A valid DOT medical card is necessary if you:

  • Hold a CDL-class A, B, or C for interstate trade
  • Operate vehicles exceeding 10,001 lbs. GVWR across state lines
  • Carry more than 8 passengers for pay or over 15 passengers unpaid
  • Transport hazardous goods needing DOT permits
  • Are employed by entities overseen by DOT divisions like FMCSA, FAA, FRA, FTA, or USCG requiring DOT medical eligibility.
Port Charlotte, AK

What to bring with you to a DOT Physical?

  • Government-issued photo identification
  • Eyewear (glasses/contact lenses) & associated prescription
  • Detailed medication list & dosages
  • CPAP usage records (if it applies)
  • Specialist medical clearances (e.g., cardiology, sleep apnea, or diabetes)
  • Recent A1C or blood pressure records if they are being tracked

Port Charlotte, CT residents must prepare these for a thorough DOT physical examination.

What happens if I fail a DOT Physical?

  • No Medical card will be issued
  • Temporary disqualification notice
  • Directed to consult your primary care provider
  • Consider FMCSA exemptions for specific health issues
  • Notification given to your employer
  • Follow-up steps: address health concerns and schedule a retake of the exam

This process is in effect in Port Charlotte, ME.

What Will Fail a DOT Physical?

Medical Conditions That Can Fail a DOT Physical

  • Vision & Hearing: Vision weaker than 20/40 in each eye even with correction, inability to recognize traffic light colors, or hearing deficiency that affects detecting a forced whisper at 5 feet.
  • Blood Pressure & Heart Disease: Unmanaged high blood pressure (≥180/110 mmHg), recent myocardial infarction, stroke, unaddressed angina, or a defibrillator implant without medical sign-off.
  • Diabetes: Uncontrolled diabetes accompanied by frequent hypoglycemia or complications like neuropathy or sight impairment that interfere with driving safety.
  • Sleep Apnea & Respiratory Issues: Untreated obstructive sleep apnea causing drowsiness during the day, or severe pulmonary conditions affecting proper oxygenation.
  • Neurological Disorders: Epilepsy/seizure conditions (unless exempt), disorders causing unexpected unconsciousness, dizziness, or uncontrolled movements.
  • Substance Abuse: Current substance misuse, alcohol dependency, or use of prescriptions that reduce driving capability.
  • Psychiatric & Cognitive Disorders: Severe, unmanaged psychiatric disorders (e.g., active schizophrenia, bipolar disorder with recent episodes) or cognitive deficits that affect judgment and reflexes.

Some conditions might temporarily disqualify you until medical clearance or documentation is provided (e.g., regulated blood pressure, managed diabetes). Others, like untreated seizure disorders or implanted defibrillators, generally result in permanent disqualification as per FMCSA standards.

Port Charlotte Drug Testing

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Port Charlotte Hair Drug Testing

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Port Charlotte Alcohol Testing

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Port Charlotte On Site Drug Testing

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Port Charlotte DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Port Charlotte, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT physical typically includes a review of the driver’s health history and medications, vision screening, hearing assessment, vital signs (blood pressure, pulse), urinalysis (checking specific gravity, protein, glucose), and a full physical examination by a certified medical examiner. Upon passing, a Medical Examiner’s Certificate (DOT medical card) may be issued for up to 24 months.
You must have a valid DOT medical certificate if you: hold a commercial driver’s license (CDL) Class A, B or C for interstate commerce; operate a vehicle over 10,001 lbs GVWR in interstate commerce; transport more than eight passengers (for hire) or over fifteen passengers (not for hire); or transport hazardous materials requiring placarding under DOT regulations.
DOT physicals are offered at test centres in all cities and all states, through Accredited Drug Testing’s nationwide network. You can schedule by calling 1-800-221-4291.
You should bring a government-issued photo ID; glasses or contact lenses and the prescription; a list of medications and dosages; CPAP usage data if applicable; and any specialist clearance letters or physician documentation (for conditions like heart disease, sleep apnea, diabetes) to your DOT physical.
If you fail the DOT physical, you will not receive the medical card; you may be temporarily or permanently disqualified depending on the condition; you will need to address any medical issues (e.g., uncontrolled hypertension, untreated sleep apnea, recent myocardial infarction) and then be re-examined.

Customer Reviews

ADT is truly the best

Where do I start?!! ADT is truly the best!! I had the pleasure of speaking with Tori today! She was excellent, professional and truly went above and beyond in her kind, compassionate care and commitment to providing me with the best service possible. I was in such a time crunch, but I was able to call, order the test, pay for it over the phone and get the test I needed within the hour. Not only did this put my mind at ease, they saved my job!! I am forever grateful for this team of hard workers, that care, with such great work ethics! Thank you from the very bottom of my heart!!! If you need a drug, alcohol, etc… screening, this team will be your lifesavers!!

Sarah Patterson - 4/8/2025

phone support has been excellent

I have used ADT several times. Their phone support has been excellent, both when putting in the orders and when asking for help to interpret results.

Tee Ess - 12/19/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291