DOT Physical Exams - Brooksville, FL

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With 32 testing centers in the Brooksville area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

(800) 221-4291

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32 DOT Drug-Testing Centers in Brooksville

Minutes Away, Test Today

20205 Cortez Blvd

1.28 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
20205 Cortez Blvd
Brooksville, FL 34601
Call (800) 221-4291 Get Donor Pass

17240 Cortez Blvd

3.29 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
17240 Cortez Blvd
Brooksville, FL 34601
Call (800) 221-4291 Get Donor Pass

12114 Cortez Blvd #205

8.37 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12114 Cortez Blvd #205
Brooksville, FL 34613
Call (800) 221-4291 Get Donor Pass

12220 Cortez Blvd

8.92 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12220 Cortez Blvd
Brooksville, FL 34613
Call (800) 221-4291 Get Donor Pass

12120 Cortez Blvd

9.10 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
12120 Cortez Blvd
Brooksville, FL 34613
Call (800) 221-4291 Get Donor Pass

31075 Cortez Blvd

9.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
31075 Cortez Blvd
Brooksville, FL 34602
Call (800) 221-4291 Get Donor Pass

7007 Nightwalker Rd,

10.06 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-3:00 PM • Sat: 6:30 AM-10:30 AM✓ Walk-in with Electronic Donor Pass
7007 Nightwalker Rd,
Brooksville, FL 34613
Call (800) 221-4291 Get Donor Pass

4112 Mariner Blvd

10.32 mi
🕑 Testing Hours: Mon–Sat: 9:00 AM-9:00 PM • Sun: 10:00 AM-4:00 PM✓ Walk-in with Electronic Donor Pass
4112 Mariner Blvd
Spring Hill, FL 34609
Call (800) 221-4291 Get Donor Pass

3041 Landover Blvd.,

10.65 mi
🕑 Testing Hours: Mon–Fri: 6:00 AM-2:00 PM • Sat: 6:00 AM-10:00 AM✓ Walk-in with Electronic Donor Pass
3041 Landover Blvd.,
Spring Hill, FL 34608
Call (800) 221-4291 Get Donor Pass

3037 Landover Blvd

10.71 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3037 Landover Blvd
Spring Hill, FL 34608
Call (800) 221-4291 Get Donor Pass

40 Seven Hills Dr,

12.16 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-3:00 PM✓ Walk-in with Electronic Donor Pass
40 Seven Hills Dr,
Spring Hill, FL 34609
Call (800) 221-4291 Get Donor Pass

11123 County Line Rd

12.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
11123 County Line Rd
Spring Hill, FL 34609
Call (800) 221-4291 Get Donor Pass

10441 Quality Dr Ste 105

12.42 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
10441 Quality Dr Ste 105
Spring Hill, FL 34609
Call (800) 221-4291 Get Donor Pass

170 Mariner Blvd., Unit 7-a

12.68 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
170 Mariner Blvd., Unit 7-a
Spring Hill, FL 34609
Call (800) 221-4291 Get Donor Pass

34498 Cortez Blvd

13.63 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
34498 Cortez Blvd
Ridge Manor, FL 33523
Call (800) 221-4291 Get Donor Pass

13933 17th St Ste 101

17.73 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
13933 17th St Ste 101
Dade City, FL 33525
Call (800) 221-4291 Get Donor Pass

117 W Belt Ave Ste A

18.51 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
117 W Belt Ave Ste A
Bushnell, FL 33513
Call (800) 221-4291 Get Donor Pass

312 S. Line Ave., Ste B

19.31 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
312 S. Line Ave., Ste B
Inverness, FL 34453
Call (800) 221-4291 Get Donor Pass

210 S. Apopka Ave,

19.46 mi
🕑 Testing Hours: Mon–Fri: 6:30 AM-2:00 PM • Sat: 6:00 AM-10:00 AM✓ Walk-in with Electronic Donor Pass
210 S. Apopka Ave,
Inverness, FL 34452
Call (800) 221-4291 Get Donor Pass

14000 Fivay Rd Injury Care Only

20.08 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14000 Fivay Rd Injury Care Only
Hudson, FL 34667
Call (800) 221-4291 Get Donor Pass

3956 S Suncoast Blvd

20.58 mi
🕑 Testing Hours: ✓ Walk-in with Electronic Donor Pass
3956 S Suncoast Blvd
Homosassa, FL 34448
Call (800) 221-4291 Get Donor Pass

1907 Hwy 44 W

21.00 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1907 Hwy 44 W
Inverness, FL 34453
Call (800) 221-4291 Get Donor Pass

27421 State Road 54

22.12 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
27421 State Road 54
Wesley Chapel, FL 33544
Call (800) 221-4291 Get Donor Pass

14100 Fivay Rd Ste 140

22.65 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14100 Fivay Rd Ste 140
Hudson, FL 34667
Call (800) 221-4291 Get Donor Pass

13944 Lakeshore Blvd Ste D

22.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
13944 Lakeshore Blvd Ste D
Hudson, FL 34667
Call (800) 221-4291 Get Donor Pass

5504 Gateway Blvd

22.75 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
5504 Gateway Blvd
Wesley Chapel, FL 33544
Call (800) 221-4291 Get Donor Pass

37802 Medical Arts Ct

23.50 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
37802 Medical Arts Ct
Zephyrhills, FL 33541
Call (800) 221-4291 Get Donor Pass

7050 Gall Blvd

23.84 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
7050 Gall Blvd
Zephyrhills, FL 33541
Call (800) 221-4291 Get Donor Pass

7459 State Road 52,

23.91 mi
🕑 Testing Hours: Mon–Fri: 5:30 AM-2:00 PM • Sat: 6:00 AM-10:30 AM✓ Walk-in with Electronic Donor Pass
7459 State Road 52,
Hudson, FL 34667
Call (800) 221-4291 Get Donor Pass

6140 W Corporate Oaks Dr,

24.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6140 W Corporate Oaks Dr,
Crystal River, FL 34429
Call (800) 221-4291 Get Donor Pass

6719 Gall Blvd., Suite 101

24.07 mi
🕑 Testing Hours: Mon–Fri: 7:00 AM-2:00 PM • Sat: 7:30 AM-11:30 AM✓ Walk-in with Electronic Donor Pass
6719 Gall Blvd., Suite 101
Zephyrhills, FL 33542
Call (800) 221-4291 Get Donor Pass

6755 Gall Blvd

24.07 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
6755 Gall Blvd
Zephyrhills, FL 33542
Call (800) 221-4291 Get Donor Pass

Brooksville, FL DOT Physical - What You Need to Know

A DOT physical is an extensive health assessment mandated by the Department of Transportation (DOT) for all operators of commercial motor vehicles (CMVs). This evaluation ensures drivers possess the necessary physical, mental, and emotional stability to safely handle large vehicles on the roads. Conducted by a certified medical examiner (CME) registered with the National Registry of Certified Medical Examiners (NRCME), clearing this exam grants drivers a DOT medical certificate (also known as a DOT medical card), essential for retaining a valid CDL license as per FMCSA guidelines. For guidance on the required DOT physical if you fall under another DOT agency's jurisdiction, call us at 800-221-4291.

During your exam, the medical examiner will complete a thorough health assessment to determine if you meet the Federal Motor Carrier Safety Administration (FMCSA) medical standards. The process typically includes:

What Does a DOT Physical Include?

Health History Review
Vision Test

Hearing Test
Urinalysis

Vital Signs
Physical Examination

DOT Testing | DOT Breath Alcohol | DOT Consortium Services | MVR Reports Also Available


What is checked in a DOT Physical?

  • Review of health history and evaluation of medication
  • Eye test (corrective eyewear permitted; minimal criteria must be met)
  • Ear examination (forced whisper or hearing test)
  • Measurement of blood pressure and pulse
  • Physical inspection of body systems
  • Urine analysis (checking specific gravity, protein, and sugar)

In Brooksville, IL, after passing, the Medical Examiner's Certificate (MEC) can be issued for up to 24 months. Certain conditions may warrant a shorter certification or necessitate specialist documentation.

Who needs a DOT Physical?

You must have a valid DOT medical certificate (card) if you:

  • Have a commercial driver's license (CDL)- class A,B, or C for interstate commerce
  • Drive a vehicle over 10,001 lbs. GVWR in interstate commerce
  • Transport more than 8 passengers (for hire) or more than 15 passengers (not for hire)
  • Transport hazardous materials that require placarding under DOT regulations
  • Work for employers regulated by DOT agencies such as FMCSA, FAA, FRA, FTA or USCG where DOT medical qualification is required

This applies specifically to Brooksville, WI.

DOT Physical Near Brooksville, FL

High Point, FL

Hill 'n Dale, FL

Brookridge, FL

Garden Grove, FL

Lake Lindsey, FL

Powell, FL

Wiscon, FL

South Brooksville, FL

North Brooksville, FL

What to bring with you to a DOT Physical?

  • Government-issued photo ID
  • Glasses/contact lenses & prescription
  • List of medications & dosages
  • CPAP usage data (if applicable)
  • Specialist clearances (cardiology, sleep apnea, diabetes, etc.)
  • Recent A1C/blood pressure logs if monitored

Make sure you bring these important documents to your exam in Brooksville, VT.

What happens if I fail a DOT Physical?

  • No Medical certification will be provided
  • Possible temporary disqualification
  • Recommendation for treatment by your primary care physician
  • FMCSA exemptions applicable for specific medical conditions
  • Employer will be informed
  • Subsequent steps involve addressing the medical conditions and retaking the examination in Brooksville, CT

What Will Fail a DOT Physical?

Medical Conditions That Can Fail a DOT Physical

  • Vision & Hearing: Vision worse than 20/40 per eye (with glasses), inability to see traffic light colors, or hearing loss preventing detection of a whisper at 5 feet.
  • Blood Pressure & Heart Disease: Uncontrolled high blood pressure (≥180/110 mmHg), recent heart attack, stroke, uncontrolled chest pain, or an unapproved defibrillator.
  • Diabetes: Poorly managed diabetes with frequent hypoglycemia or complications (nerve damage, vision issues) disrupting safe driving.
  • Sleep Apnea & Respiratory Issues: Untreated sleep apnea causing daytime sleepiness, or severe lung disease impairing breathing.
  • Neurological Disorders: Epilepsy/seizures (unless exempted), conditions causing sudden unconsciousness, dizziness, or uncontrollable shakes.
  • Substance Abuse: Current illicit drug use, alcohol dependency, or misuse of prescription drugs impairing driving.
  • Psychiatric & Cognitive Disorders: Severe unchecked mental illness (e.g., schizophrenia, bipolar disorder with recent episodes) or cognitive decline affecting decision-making and response time.

Issues may temporarily disqualify you until you provide clearance or documentation (e.g., controlled blood pressure, diabetes management), while others like untreated seizures or unauthorized defibrillators are typically considered permanent disqualifiers by FMCSA.

References:
- FMCSA Medical Examiner Physical Qualification Standards (§391.41)
- 49 CFR Part 391 - Qualification of Drivers
- Medical Examiner's Handbook
- FMCSA Driver Exemptions

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Brooksville 5 Panel Drug Test

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Brooksville DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Brooksville, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT physical typically includes a review of the driver’s health history and medications, vision screening, hearing assessment, vital signs (blood pressure, pulse), urinalysis (checking specific gravity, protein, glucose), and a full physical examination by a certified medical examiner. Upon passing, a Medical Examiner’s Certificate (DOT medical card) may be issued for up to 24 months.
You must have a valid DOT medical certificate if you: hold a commercial driver’s license (CDL) Class A, B or C for interstate commerce; operate a vehicle over 10,001 lbs GVWR in interstate commerce; transport more than eight passengers (for hire) or over fifteen passengers (not for hire); or transport hazardous materials requiring placarding under DOT regulations.
DOT physicals are offered at test centres in all cities and all states, through Accredited Drug Testing’s nationwide network. You can schedule by calling 1-800-221-4291.
You should bring a government-issued photo ID; glasses or contact lenses and the prescription; a list of medications and dosages; CPAP usage data if applicable; and any specialist clearance letters or physician documentation (for conditions like heart disease, sleep apnea, diabetes) to your DOT physical.
If you fail the DOT physical, you will not receive the medical card; you may be temporarily or permanently disqualified depending on the condition; you will need to address any medical issues (e.g., uncontrolled hypertension, untreated sleep apnea, recent myocardial infarction) and then be re-examined.

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(800) 221-4291