DOT Physical Exams - Mayo, FL

Locations, All Cities, All States

FMCSA | FAA | USCG | FTA | FRA | PHMSA

With 24 testing centers in the Mayo area, we offer drug and alcohol testing for employment, probation and DOT requirements. Additional services include DOT consortium enrollment, DNA Testing and background checks. Order your tests and services online or call (800) 221-4291.

24 DOT Drug-Testing Centers in Mayo

Minutes Away, Test Today

1506 Ohio Ave S Ste 2

19.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1506 Ohio Ave S Ste 2
Live Oak, FL 32064

215 Howard St W

20.27 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
215 Howard St W
Live Oak, FL 32064

3498 Hwy 19 South #2

23.20 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3498 Hwy 19 South #2
Perry, FL 32348

3470 Nw 57th Trl

24.40 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3470 Nw 57th Trl
Bell, FL 32619

315 E Ash St

24.87 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
315 E Ash St
Perry, FL 32347

333 N Byron Butler Pkwy

25.26 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
333 N Byron Butler Pkwy
Perry, FL 32347

221 Sw Stonegate Terrace, Ste 107

29.33 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
221 Sw Stonegate Terrace, Ste 107
Lake City, FL 32024

256 Sw Washington Ave

32.01 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
256 Sw Washington Ave
Madison, FL 32340

224 Nw Crane Ave Attn Lab

32.11 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
224 Nw Crane Ave Attn Lab
Madison, FL 32340

228 Ne Hancock Ave

32.23 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
228 Ne Hancock Ave
Madison, FL 32340

265 Sw Malone Street,suite 105

32.81 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
265 Sw Malone Street,suite 105
Lake City, FL 32025

260 S Marion Ave Bldg B Ste 125

33.21 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
260 S Marion Ave Bldg B Ste 125
Lake City, FL 32025

325 E Baya Ave

33.53 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
325 E Baya Ave
Lake City, FL 32025

413 5th Ave Nw Ste 3

34.97 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
413 5th Ave Nw Ste 3
Jasper, FL 32052

4134 W Us Highway 90

35.32 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4134 W Us Highway 90
Lake City, FL 32055

4367 Nw American Ln

36.16 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
4367 Nw American Ln
Lake City, FL 32055

3824 W Us Highway 90

37.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3824 W Us Highway 90
Lake City, FL 32055

3824 E Us Highway 90

37.03 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
3824 E Us Highway 90
Lake City, FL 32055

420 Ne Santa Fe Blvd

38.30 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
420 Ne Santa Fe Blvd
High Springs, FL 32643

1113 Nw 23rd Ave

42.24 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
1113 Nw 23rd Ave
Chiefland, FL 32626

14417 Nw 152nd Ln

44.02 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14417 Nw 152nd Ln
Alachua, FL 32615

128 Nw 137th Dr

44.25 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
128 Nw 137th Dr
Newberry, FL 32669

14819 Nw 140th St

44.62 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
14819 Nw 140th St
Alachua, FL 32615

850 E Main St Attn Lab

46.70 mi
🕑 Mon–Fri: 8:00 AM – 4:00 PM✓ Walk-in with Electronic Donor Pass
850 E Main St Attn Lab
Lake Butler, FL 32054

Mayo, FL DOT Physical - What You Need to Know

A DOT physical is a detailed health evaluation mandated by the Department of Transportation for all commercial vehicle operators. This examination verifies that drivers are healthy enough, both physically and mentally, to operate large vehicles safely on public pathways. A certified medical examiner listed on the National Registry of Certified Medical Examiners conducts the DOT physical. Passing it enables drivers to obtain a DOT medical card, essential for maintaining a valid CDL under FMCSA guidelines. If uncertain about which DOT physical applies due to regulation by another DOT entity, call us at 800-221-4291.

During your exam, the medical examiner will complete a thorough health assessment to determine if you meet the Federal Motor Carrier Safety Administration (FMCSA) medical standards. The process typically includes:

What Does a DOT Physical Include?

Health History Review
Vision Test

Hearing Test
Urinalysis

Vital Signs
Physical Examination

DOT Testing | DOT Breath Alcohol | DOT Consortium Services | MVR Reports Also Available


What is checked in a DOT Physical?

  • Review of medical history and medication list
  • Eye test with optional corrective lenses to meet minimum vision requirements
  • Hearing test either through audiometry or forced whisper check
  • Pulse rate and blood pressure assessment
  • Physical evaluation of bodily systems
  • Urine test for specific gravity, protein, and sugar

After passing, a Medical Examiner's Certificate (MEC) might be granted for a period of up to 24 months in Mayo, SD. Certain conditions could necessitate a shorter certification period or further specialist evaluation.

Who needs a DOT Physical?

In Mayo, CO, a valid DOT medical certificate (card) is necessary if you:

  • Hold a commercial driver's license (CDL) - class A, B, or C for interstate commerce
  • Operate a vehicle over 10,001 lbs. GVWR in interstate commerce
  • Transport over 8 passengers (for hire) or over 15 passengers (not for hire)
  • Transport hazardous materials requiring placarding under DOT rules
  • Are employed by entities under DOT agencies like FMCSA, FAA, FRA, FTA, or USCG needing DOT medical qualification

What to bring with you to a DOT Physical?

  • Official photo ID issued by the government
  • Prescription glasses/contact lenses with prescription details
  • List of all medications and dosages
  • Data on CPAP usage (if applicable)
  • Clearances from specialists (e.g., cardiology, sleep apnea, diabetes, etc.)
  • Recent A1C/blood pressure logs if applicable

Ensure to bring these documents to your DOT physical in Mayo, AL to facilitate the process.

What happens if I fail a DOT Physical?

  • No Medical card awarded
  • Temporary suspension
  • Refer to your primary doctor for treatment
  • FMCSA offers exceptions for specific medical cases
  • Employer will be notified
  • Next steps involve addressing medical concerns and reattempting the exam. Ensure complete adherence to Mayo, NM's operational standards for effective results.

What Will Fail a DOT Physical?

Medical Conditions That Can Fail a DOT Physical

  • Vision & Hearing: Visual acuity worse than 20/40 in either eye (even with glasses), difficulty identifying traffic lights, or inability to hear a whispered voice within 5 feet.
  • Blood Pressure & Heart Disease: Severe hypertension (≥180/110 mmHg), recent heart attack or stroke, unstable angina, or an unapproved defibrillator.
  • Diabetes: Frequent hypoglycemic events or complications like neuropathy impacting driving safety from uncontrolled diabetes.
  • Sleep Apnea & Respiratory Issues: Untreated sleep apnea resulting in daytime sleepiness or severe lung conditions affecting breathing.
  • Neurological Disorders: Epilepsy or seizures (unless exempt), conditions causing sudden blackouts or involuntary tremors.
  • Substance Abuse: Current illegal drug use, alcohol abuse, or misuse of prescription drugs affecting motor skills.
  • Psychiatric & Cognitive Disorders: Severe mental health issues (e.g., uncontrolled schizophrenia) or cognitive deficits harming decision-making skills.

Some conditions may temporarily prevent certification until resolved with documentation (e.g., controlled blood pressure). Other issues, like unmanaged seizures, usually lead to permanent ineligibility under FMCSA norms.

Mayo Drug Testing

Quickly find trusted local drug testing centers in Mayo, FL — fast, convenient, and reliable every time!

Mayo DOT Drug Testing

Quickly find a local DOT drug testing center in Mayo, FL — fast, reliable, convenient nationwide service!

Mayo DNA Testing

DNA testing for legal and non-legal purposes including child support, and child custody around Mayo, FL.

Mayo Industry Training

Including specimen collector training, DER training, reasonable suspicion training and much more in Mayo, FL.v

Mayo Hair Drug Testing

Get fast, accurate hair drug testing at our convenient Mayo, FL locations—results you can trust, every time!

Mayo Alcohol Testing

Fast, reliable alcohol testing to keep your workplace safe and compliant every single day in Mayo, FL.

Mayo Drug Testing Services

Reliable nationwide drug testing services ensuring accuracy, compliance, and quick results for businesses and individuals in Mayo, FL.

Mayo 5 Panel Drug Test

Accurate 5 panel drug testing with fast, confidential results—trusted by Mayo, FL employers and individuals nationwide.

Mayo 10 Panel Drug Test

Comprehensive 10 panel drug testing with fast, reliable results—trusted for Mayo, FL workplace and personal testing needs.

Mayo On Site Drug Testing

Convenient on-site mobile drug testing with fast, reliable results—saving time while keeping your Mayo, FL workplace compliant.

Mayo DOT Physicals

Certified DOT physicals with quick, reliable exams to keep Mayo, FL drivers compliant and on the road with confidence.

DOT Consortium

Join our DOT consortium for hassle-free compliance, random testing management, and reliable driver safety solutions.


Frequently Asked Questions

A DOT physical typically includes a review of the driver’s health history and medications, vision screening, hearing assessment, vital signs (blood pressure, pulse), urinalysis (checking specific gravity, protein, glucose), and a full physical examination by a certified medical examiner. Upon passing, a Medical Examiner’s Certificate (DOT medical card) may be issued for up to 24 months.
You must have a valid DOT medical certificate if you: hold a commercial driver’s license (CDL) Class A, B or C for interstate commerce; operate a vehicle over 10,001 lbs GVWR in interstate commerce; transport more than eight passengers (for hire) or over fifteen passengers (not for hire); or transport hazardous materials requiring placarding under DOT regulations.
DOT physicals are offered at test centres in all cities and all states, through Accredited Drug Testing’s nationwide network. You can schedule by calling 1-800-221-4291.
You should bring a government-issued photo ID; glasses or contact lenses and the prescription; a list of medications and dosages; CPAP usage data if applicable; and any specialist clearance letters or physician documentation (for conditions like heart disease, sleep apnea, diabetes) to your DOT physical.
If you fail the DOT physical, you will not receive the medical card; you may be temporarily or permanently disqualified depending on the condition; you will need to address any medical issues (e.g., uncontrolled hypertension, untreated sleep apnea, recent myocardial infarction) and then be re-examined.

Customer Reviews

ADT is truly the best

Where do I start?!! ADT is truly the best!! I had the pleasure of speaking with Tori today! She was excellent, professional and truly went above and beyond in her kind, compassionate care and commitment to providing me with the best service possible. I was in such a time crunch, but I was able to call, order the test, pay for it over the phone and get the test I needed within the hour. Not only did this put my mind at ease, they saved my job!! I am forever grateful for this team of hard workers, that care, with such great work ethics! Thank you from the very bottom of my heart!!! If you need a drug, alcohol, etc… screening, this team will be your lifesavers!!

Sarah Patterson - 4/8/2025

phone support has been excellent

I have used ADT several times. Their phone support has been excellent, both when putting in the orders and when asking for help to interpret results.

Tee Ess - 12/19/2024

quick to assist

They are very quick to assist with your orders and they helped when I needed to find a new testing facility.

Gary Matkin - 2/19/2025


(800) 221-4291